FastQCFastQC Report
Wed 25 May 2016
SRR491027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18508055
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA127450.040.741841
TCTCGAG146100.035.389312
CTCGAGG191200.027.0949213
GATCCGG188450.026.3049321
TCGAGGG228550.022.6875954
ATCCGGC219650.022.4264072
TATCTCG68750.021.5011621
TCCGGCG248400.019.8715573
CGAGGGC293750.017.5238885
GCGCCGT287900.017.0775019
GGATCCG127200.016.8872412
ATATCTC92450.016.4984471
CGGCGCG319200.015.316685
AGGGCGC337800.015.2916257
CGTCGAC269450.015.11473912-13
GAGGGCG353100.014.6026846
CCGGCGC340500.014.5794454
GCCGTCG283650.014.46571710-11
TCGACTT288850.014.15816714-15
TATGGAT115450.014.1087041
Produced by FastQC (version 0.11.5)