##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491027_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18508055
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.698277263602254	33.0	31.0	34.0	29.0	34.0
2	31.94115308172577	34.0	31.0	34.0	30.0	34.0
3	32.002149064285796	34.0	31.0	34.0	30.0	34.0
4	35.364797597586566	37.0	35.0	37.0	33.0	37.0
5	35.14564950233831	37.0	35.0	37.0	32.0	37.0
6	35.081973983760044	37.0	35.0	37.0	32.0	37.0
7	35.004175695393165	37.0	35.0	37.0	32.0	37.0
8	34.92246527255295	37.0	35.0	37.0	32.0	37.0
9	36.447775576634065	39.0	37.0	39.0	32.0	39.0
10-11	36.277524812845	39.0	37.0	39.0	32.0	39.0
12-13	36.12833142110287	39.0	37.0	39.0	32.0	39.0
14-15	37.42012999204941	40.0	38.0	41.0	32.0	41.0
16-17	37.29390973281633	40.0	38.0	41.0	31.5	41.0
18-19	37.13970592804052	40.0	38.0	41.0	31.0	41.0
20-21	36.98285624826596	40.0	38.0	41.0	30.5	41.0
22-23	36.77609540818849	40.0	37.5	41.0	30.0	41.0
24-25	36.55985853186626	40.0	37.0	41.0	29.5	41.0
26-27	36.195735937676865	40.0	36.5	41.0	28.0	41.0
28-29	35.86561048689341	40.0	36.0	41.0	27.0	41.0
30-31	35.555908954236415	39.5	35.5	41.0	26.5	41.0
32-33	35.319707878542616	39.0	35.0	41.0	25.0	41.0
34-35	35.092229248292156	39.0	35.0	41.0	24.0	41.0
36-37	34.77055849466625	39.0	35.0	40.5	23.0	41.0
38-39	34.41952795688148	39.0	35.0	40.0	19.5	41.0
40-41	33.938786517546006	38.5	34.0	40.0	14.5	41.0
42-43	33.51525276426939	38.0	33.0	40.0	8.5	41.0
44-45	33.02571904503201	38.0	33.0	40.0	2.0	41.0
46-47	32.45677441524785	38.0	32.5	40.0	2.0	41.0
48-49	31.92272270100775	38.0	31.0	40.0	2.0	41.0
50-51	31.45541814091216	37.5	30.5	40.0	2.0	41.0
52-53	30.893799429491644	37.0	29.5	40.0	2.0	41.0
54-55	30.363769477667965	36.5	28.5	39.5	2.0	41.0
56-57	29.849923965538245	36.0	28.0	39.0	2.0	40.0
58-59	29.307010136937674	35.5	26.5	39.0	2.0	40.0
60-61	28.7350446602844	35.0	26.0	38.5	2.0	40.0
62-63	28.105562253840287	34.5	24.0	38.0	2.0	40.0
64-65	27.462657691475414	34.0	22.0	38.0	2.0	40.0
66-67	26.842943194193012	34.0	19.5	37.5	2.0	39.5
68-69	26.11813634657991	33.0	15.5	37.0	2.0	39.0
70-71	25.349693984592115	32.0	7.5	36.0	2.0	39.0
72-73	24.60649835976822	31.5	4.0	35.5	2.0	38.5
74-75	23.807754353442327	31.0	2.0	35.0	2.0	38.0
76-77	22.82080526559922	29.5	2.0	34.0	2.0	36.0
78-79	22.804068390762833	30.0	2.0	34.0	2.0	36.0
80-81	22.360621307857578	30.0	2.0	34.0	2.0	36.0
82-83	21.789159125580724	29.0	2.0	34.0	2.0	35.5
84-85	20.994251178743525	29.0	2.0	34.0	2.0	35.0
86-87	20.275739455064297	27.5	2.0	34.0	2.0	35.0
88-89	19.631100998997464	26.5	2.0	33.0	2.0	35.0
90-91	18.796125254652637	25.5	2.0	33.0	2.0	34.5
92-93	17.994635849093815	24.5	2.0	32.0	2.0	34.0
94-95	17.05433382924354	19.5	2.0	32.0	2.0	34.0
96-97	16.063917008027044	9.0	2.0	31.0	2.0	34.0
98-99	15.121867640873123	2.0	2.0	31.0	2.0	34.0
100	14.286992663464638	2.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18514158522575386
1101	2	0.22152921575083084
1101	3	0.18580238905890667
1101	4	0.18065932353365355
1101	5	0.21192866830563872
1101	6	0.24553927342616078
1101	7	0.24827045383153745
1101	8	0.2638103002950061
1101	9	0.29236742782919833
1101	10-11	0.3029746315163422
1101	12-13	0.3188827375444703
1101	14-15	0.3567289530123716
1101	16-17	0.33726933391159974
1101	18-19	0.3618017666342723
1101	20-21	0.32266795782214786
1101	22-23	0.2881272064440594
1101	24-25	0.29936566260243325
1101	26-27	0.29588420244841274
1101	28-29	0.27864797060933455
1101	30-31	0.2850026416879885
1101	32-33	0.25947814412673154
1101	34-35	0.25902209069330695
1101	36-37	0.24675933929299276
1101	38-39	0.19761873026403265
1101	40-41	0.21641165213503655
1101	42-43	0.07097436578171568
1101	44-45	-0.011434533173812156
1101	46-47	0.021535147317329972
1101	48-49	0.05206204054341512
1101	50-51	-0.0888350669698248
1101	52-53	-0.2106002347187541
1101	54-55	-0.23866744207841606
1101	56-57	-0.26118163683732476
1101	58-59	-0.3876716408159382
1101	60-61	-0.5052060060711092
1101	62-63	-0.5738505448937197
1101	64-65	-0.49090633041288
1101	66-67	-0.505942054020462
1101	68-69	-0.6160432399503151
1101	70-71	-0.5690112601889581
1101	72-73	-0.6898640127715261
1101	74-75	-0.6861381135719995
1101	76-77	-0.5502332208114886
1101	78-79	-0.6004927961341942
1101	80-81	-0.7073887016554821
1101	82-83	-0.7017677066935413
1101	84-85	-0.7822073727947441
1101	86-87	-0.9270272581195229
1101	88-89	-0.7962458073303615
1101	90-91	-0.788629089588504
1101	92-93	-0.7586027256186831
1101	94-95	-0.7600698105878614
1101	96-97	-0.6989454303745024
1101	98-99	-0.7894018782139405
1101	100	-0.8885135798447497
1102	1	0.13340258246788395
1102	2	0.14990761283761245
1102	3	0.15144214787493127
1102	4	0.128378452980769
1102	5	0.15011724114832958
1102	6	0.1255976374630947
1102	7	0.1389968104102195
1102	8	0.1355685985595798
1102	9	0.13490928553460435
1102	10-11	0.10972294809921124
1102	12-13	0.12112088019944878
1102	14-15	0.13066257125924352
1102	16-17	0.145900586013326
1102	18-19	0.17284445658017944
1102	20-21	0.12013238191286746
1102	22-23	0.16553027795866626
1102	24-25	0.20719225935584973
1102	26-27	0.1476343548761605
1102	28-29	0.11653000961717908
1102	30-31	0.09918792507182417
1102	32-33	0.0953510597364513
1102	34-35	0.008052287949645631
1102	36-37	0.09423804866041507
1102	38-39	0.05206250159273651
1102	40-41	0.10201917262197924
1102	42-43	-0.01978037724399684
1102	44-45	-0.052791340977044854
1102	46-47	0.06680559471015357
1102	48-49	0.10563307739697692
1102	50-51	-0.02284324532244497
1102	52-53	-0.03270229508207123
1102	54-55	-0.07073621862044632
1102	56-57	-0.08713887395920139
1102	58-59	-0.20246607997163224
1102	60-61	-0.16075887074222095
1102	62-63	-0.15148794032039703
1102	64-65	-0.17751917348729762
1102	66-67	-0.21037681428490984
1102	68-69	-0.27081997561197824
1102	70-71	-0.2922475795675723
1102	72-73	-0.28490057586057915
1102	74-75	-0.18545590635181952
1102	76-77	-0.15588935864296616
1102	78-79	-0.19793964132885478
1102	80-81	-0.28091306667589677
1102	82-83	-0.2048289207694758
1102	84-85	-0.23102372579756292
1102	86-87	-0.27592877227549906
1102	88-89	-0.17138341161628645
1102	90-91	0.00290111270539839
1102	92-93	0.03096687074028992
1102	94-95	-0.0440541710856337
1102	96-97	-0.07856377585177654
1102	98-99	-0.002970141802297377
1102	100	-0.039325072859950794
1103	1	0.075292708714219
1103	2	0.07982347096733733
1103	3	0.06724072509407364
1103	4	0.060190471933069034
1103	5	0.12559356846910674
1103	6	0.11053133487541089
1103	7	0.10147077531128446
1103	8	0.06843414008007898
1103	9	0.09109847384230108
1103	10-11	0.09902389608995321
1103	12-13	0.10893468175239462
1103	14-15	0.11928823619955864
1103	16-17	0.15623593649524992
1103	18-19	0.20146347700268308
1103	20-21	0.18499004023030352
1103	22-23	0.160364557016905
1103	24-25	0.1507117924577699
1103	26-27	0.22225942735768456
1103	28-29	0.2204696457421491
1103	30-31	0.2266238287405784
1103	32-33	0.1064150860288322
1103	34-35	0.0837950054271559
1103	36-37	0.06948175537198154
1103	38-39	0.1097579614870412
1103	40-41	0.14158833943393745
1103	42-43	0.028878333733800332
1103	44-45	-0.09415508019147723
1103	46-47	0.010187791512230149
1103	48-49	-0.12060953807941388
1103	50-51	0.003018265236764961
1103	52-53	-0.12913453303846723
1103	54-55	-0.15275365760567894
1103	56-57	-0.06765345537316136
1103	58-59	-0.0742113086979117
1103	60-61	-0.21143240170234634
1103	62-63	-0.06302363177556813
1103	64-65	-0.03146887580282964
1103	66-67	-0.04395669053048579
1103	68-69	-0.07080876312905815
1103	70-71	-0.10475284292408205
1103	72-73	-0.17420043639843286
1103	74-75	-0.19315202425373457
1103	76-77	-0.14424609719669235
1103	78-79	-0.11851999304045435
1103	80-81	-0.134238230427556
1103	82-83	-0.05861594238704271
1103	84-85	-0.1937982009535375
1103	86-87	-0.14287957884051394
1103	88-89	0.05905298655928348
1103	90-91	0.24276913978087578
1103	92-93	0.35012719229365885
1103	94-95	0.18313279355079004
1103	96-97	0.21807902818803981
1103	98-99	0.21003467414052324
1103	100	0.27774159840426194
1104	1	-0.05083637013022013
1104	2	-0.039351406502589725
1104	3	-0.05865724523233595
1104	4	-0.048347638468925425
1104	5	-0.036920250911293806
1104	6	-0.045245136058980506
1104	7	-0.057576412586598735
1104	8	-0.04890088195809028
1104	9	-0.10424180771558866
1104	10-11	-0.09448269194012227
1104	12-13	-0.04169651338914804
1104	14-15	-0.0037863072036472545
1104	16-17	0.04641886956852659
1104	18-19	0.026252183452506017
1104	20-21	0.010880126227377218
1104	22-23	0.021478958609812082
1104	24-25	0.05578640606027818
1104	26-27	0.10520157731757251
1104	28-29	-0.014714673250665555
1104	30-31	0.011211976241433774
1104	32-33	0.018062975630044775
1104	34-35	-0.004625114047826173
1104	36-37	0.04486128520183286
1104	38-39	0.07935200607689552
1104	40-41	0.16709360967280418
1104	42-43	0.09775592626552765
1104	44-45	0.03089237369958653
1104	46-47	0.1007571136398866
1104	48-49	0.09890999456853322
1104	50-51	0.03962832157519003
1104	52-53	4.319034500817054E-4
1104	54-55	0.018587263956256095
1104	56-57	0.07560649040280865
1104	58-59	0.05388418449664201
1104	60-61	0.05042457523532562
1104	62-63	0.027198524136348823
1104	64-65	0.05065891476566975
1104	66-67	0.04333090827301689
1104	68-69	0.03938507329096552
1104	70-71	0.03210049583773156
1104	72-73	0.051495574092410124
1104	74-75	0.20973253191184682
1104	76-77	0.22700754520271005
1104	78-79	0.15486535047214645
1104	80-81	0.12911584479731886
1104	82-83	0.22911329303360262
1104	84-85	0.2260498447875392
1104	86-87	0.20518051108540192
1104	88-89	0.2846105345521117
1104	90-91	0.3834900980900997
1104	92-93	0.3633424261655058
1104	94-95	0.3726932967445009
1104	96-97	0.4549063931945767
1104	98-99	0.4419136966599204
1104	100	0.5357425605589601
1105	1	-0.09169454962368562
1105	2	-0.08626810103709559
1105	3	-0.13719450300179759
1105	4	-0.08993726662242096
1105	5	-0.10077296221897569
1105	6	-0.1367866716073678
1105	7	-0.15155110936539984
1105	8	-0.05644131730131363
1105	9	-0.1245958356939667
1105	10-11	-0.1383201925693669
1105	12-13	-0.15022320236588627
1105	14-15	-0.10787586177167441
1105	16-17	-0.14465425453774117
1105	18-19	-0.07197662422490936
1105	20-21	-0.08173616248237181
1105	22-23	-0.06940225800678945
1105	24-25	-0.10349241862866165
1105	26-27	-0.24296690485115846
1105	28-29	-0.1745375527795332
1105	30-31	-0.05811897263405541
1105	32-33	-0.08836918081589573
1105	34-35	-0.10825978719287122
1105	36-37	-0.08800334541311372
1105	38-39	-0.021428090480689832
1105	40-41	0.02786534612836533
1105	42-43	-0.026614030731785476
1105	44-45	-0.061519610706618266
1105	46-47	0.05232314440190322
1105	48-49	0.008461479304767039
1105	50-51	0.02541061210709117
1105	52-53	-0.024827766807263174
1105	54-55	-0.031002929562170323
1105	56-57	0.005789967658522244
1105	58-59	0.03505858269038953
1105	60-61	0.07485685930976516
1105	62-63	0.06025292187489839
1105	64-65	0.06859112595511618
1105	66-67	-0.007023253908929661
1105	68-69	0.024238960733967474
1105	70-71	0.11046290753953159
1105	72-73	0.10957673014515734
1105	74-75	0.2870926638486182
1105	76-77	0.28335483713726717
1105	78-79	0.16970177529912078
1105	80-81	0.14778187293650547
1105	82-83	0.14049476873998756
1105	84-85	0.17315063386053708
1105	86-87	0.22323685350768585
1105	88-89	0.3458135214496494
1105	90-91	0.32016288708032903
1105	92-93	0.2699222279338791
1105	94-95	0.36099542785191474
1105	96-97	0.49340315260658585
1105	98-99	0.4927327362284899
1105	100	0.6138297056955864
1106	1	-0.20870195802862312
1106	2	-0.18043875880709237
1106	3	-0.2042760158645187
1106	4	-0.21344814889894792
1106	5	-0.22428981192883413
1106	6	-0.22779725728366884
1106	7	-0.23287734282342853
1106	8	-0.22337540362617148
1106	9	-0.2628812017675415
1106	10-11	-0.295547066126602
1106	12-13	-0.29161740630734556
1106	14-15	-0.3447050110323815
1106	16-17	-0.4451446454416512
1106	18-19	-0.5843124612754593
1106	20-21	-0.5245364835417661
1106	22-23	-0.5442612733921379
1106	24-25	-0.6679441495946321
1106	26-27	-0.7568708749873707
1106	28-29	-0.5848547765085499
1106	30-31	-0.6750430497679147
1106	32-33	-0.6525210105943415
1106	34-35	-0.5170355610774919
1106	36-37	-0.550236614661145
1106	38-39	-0.4441490805107051
1106	40-41	-0.5455500474766595
1106	42-43	-0.6496074687873943
1106	44-45	-0.5762220107443881
1106	46-47	-0.48358466802559974
1106	48-49	-0.49629446338365923
1106	50-51	-0.5167361270437176
1106	52-53	-0.6252834896673605
1106	54-55	-0.5741648455369983
1106	56-57	-0.4034837460760663
1106	58-59	-0.458248326710887
1106	60-61	-0.41237557194794405
1106	62-63	-0.43366449959791353
1106	64-65	-0.44186446423924863
1106	66-67	-0.5303046902740896
1106	68-69	-0.4776745706059842
1106	70-71	-0.49943958481614104
1106	72-73	-0.5381598093504003
1106	74-75	-0.23244169158786576
1106	76-77	-0.31021091698859493
1106	78-79	-0.38921464937178385
1106	80-81	-0.3700776035484168
1106	82-83	-0.3240005046802068
1106	84-85	-0.3845591206624732
1106	86-87	-0.28107700208198594
1106	88-89	-0.06841141672543571
1106	90-91	-0.22861858658676937
1106	92-93	-0.18902153010085954
1106	94-95	-0.20739170318813294
1106	96-97	-0.16635920968786877
1106	98-99	-0.28637341377024406
1106	100	-0.20586653966810253
1107	1	-0.25628741279243883
1107	2	-0.17462344602548185
1107	3	-0.23375940838047526
1107	4	-0.17579746521717965
1107	5	-0.18103490787207477
1107	6	-0.19776695274451583
1107	7	-0.20059079076695951
1107	8	-0.19932313402290447
1107	9	-0.21500805380640742
1107	10-11	-0.19695176384620794
1107	12-13	-0.24338012678160226
1107	14-15	-0.20079186758324852
1107	16-17	-0.21686058142822873
1107	18-19	-0.22103804391822024
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2207	90-91	0.825775184624927
2207	92-93	0.9430994386883462
2207	94-95	1.0499725903313362
2207	96-97	1.0041596835860993
2207	98-99	1.0579365269503391
2207	100	1.0376378672604503
2208	1	-0.8231200536684469
2208	2	-0.6561201329077022
2208	3	-0.6952475244985052
2208	4	-0.6400387075868821
2208	5	-0.7438697180595284
2208	6	-0.7158148886952063
2208	7	-0.7709161999928611
2208	8	-0.7502812656980566
2208	9	-0.8578061716310046
2208	10-11	-0.9954513571151722
2208	12-13	-1.0171393449864112
2208	14-15	-1.1324598131028836
2208	16-17	-1.0372538795012858
2208	18-19	-1.0642585340168083
2208	20-21	-1.190017956803807
2208	22-23	-1.0351868874520846
2208	24-25	-1.1347960154089094
2208	26-27	-1.494046742681519
2208	28-29	-1.3935382641324097
2208	30-31	-1.3739343623988987
2208	32-33	-1.4441408893273007
2208	34-35	-1.1170917117715646
2208	36-37	-1.4959506952021542
2208	38-39	-1.4766307377014911
2208	40-41	-2.1223686072823824
2208	42-43	-1.2774438357076008
2208	44-45	-0.9694021326270601
2208	46-47	-1.550538523602036
2208	48-49	-1.6555089316304112
2208	50-51	-1.5933634527631249
2208	52-53	-0.9587739721494124
2208	54-55	-0.7873858956288657
2208	56-57	-0.6815960674775425
2208	58-59	-0.8577548926724603
2208	60-61	-0.7381289642923328
2208	62-63	-0.906704950931779
2208	64-65	-0.9488644492565435
2208	66-67	-0.8659762349094642
2208	68-69	-1.2775811051005022
2208	70-71	-1.3083104818159903
2208	72-73	-1.035695405875959
2208	74-75	-1.839549079171519
2208	76-77	-1.3578019948871685
2208	78-79	-1.7393965706790802
2208	80-81	-1.7129368841920964
2208	82-83	-1.6542934677157497
2208	84-85	-2.2812697560419934
2208	86-87	-2.098904708359491
2208	88-89	-2.1835661499098187
2208	90-91	-2.2681236208443494
2208	92-93	-2.8915863810002396
2208	94-95	-3.0115548795125235
2208	96-97	-2.989624504410415
2208	98-99	-3.011816211766348
2208	100	-3.0209584912132925
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	265001.0
3	119864.0
4	117426.0
5	97278.0
6	89440.0
7	90986.0
8	94805.0
9	106183.0
10	122076.0
11	136433.0
12	152132.0
13	176180.0
14	213215.0
15	269539.0
16	322253.0
17	326413.0
18	301337.0
19	284829.0
20	288091.0
21	298861.0
22	309215.0
23	324783.0
24	339946.0
25	360293.0
26	385835.0
27	418126.0
28	463610.0
29	523858.0
30	600504.0
31	693689.0
32	818248.0
33	998321.0
34	1282332.0
35	1755217.0
36	2327633.0
37	2218277.0
38	788633.0
39	27193.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.057175831330476	27.930738621802643	27.960370245953303	23.051715300913582
2	23.847070910476546	27.536053896533158	26.984845247109973	21.632029945880323
3	23.50558805993052	26.152876914095245	26.812972825189856	23.52856220078437
4	24.06378305914125	25.82032707446103	26.707395851091448	23.408494015306267
5	24.503472809922698	26.065886005887055	26.49811471091838	22.93252647327187
6	24.47201361185991	26.010517960663282	25.813715494189076	23.70375293328773
7	24.449543036688954	26.33129206538966	26.08378209115752	23.135382806763864
8	23.999302548006966	26.131458894179815	26.30802641996563	23.561212137847594
9	23.92423500047797	25.833928524048254	26.448007548983473	23.793828926490303
10-11	23.813388300703213	26.087986513903484	26.30217825312906	23.796446932264242
12-13	23.56633669459507	26.14969526253823	26.465510378077177	23.818457664789523
14-15	23.356587265250393	26.36250780208925	26.90515388278594	23.37575104987442
16-17	23.130242357697732	26.309437723625145	27.113386685351248	23.44693323332588
18-19	22.773823235777257	26.24868755387057	27.732767124745767	23.244722085606405
20-21	22.5479742609835	25.976448716688555	28.47353437790397	23.002042644423973
22-23	22.47016722306102	25.96385581880856	28.8437572034886	22.722219754641813
24-25	22.484542076396323	25.8017289619099	29.201138917166343	22.512590044527432
26-27	22.5243400756237	25.815387265388708	29.243886169157324	22.416386489830266
28-29	22.52612241700233	25.89996244877229	29.20777175337218	22.366143380853195
30-31	22.517026331317897	25.83167334471349	29.15947977639741	22.491820547571205
32-33	22.547201148100076	25.870912497218974	29.139877282215647	22.442009072465307
34-35	22.587152049232742	25.95532622857221	29.085961219456806	22.37156050273824
36-37	22.608805170703853	25.900223669515153	29.01578740500705	22.475183754773944
38-39	22.658452383171497	25.93235467572005	28.897758024163057	22.511434916945397
40-41	22.74148219693466	26.0804808820313	28.64957544441745	22.52846147661659
42-43	22.8813324424708	26.19516221878369	28.21047124401864	22.71303409472687
44-45	23.02202452255266	26.369750113511824	27.792103877767126	22.816121486168388
46-47	23.184853354790256	26.585414896971848	27.350409339984914	22.879322408252982
48-49	23.30775842838676	26.610854430308787	27.00904910031082	23.072338040993635
50-51	23.390453283770373	26.638343353936477	26.825263493692702	23.145939868600447
52-53	23.439243584500545	26.709918706092367	26.70768994364554	23.14314776576155
54-55	23.48379438915355	26.649538922566123	26.627087734245798	23.239578954034528
56-57	23.515686561828797	26.664972247400776	26.605094842092658	23.21424634867777
58-59	23.519185320254614	26.73804166964305	26.573799390343204	23.168973619759132
60-61	23.51467280527127	26.691753515804233	26.47321863721508	23.320355041709416
62-63	23.52565536108893	26.786282669178302	26.423755712596332	23.26430625713644
64-65	23.56683126496288	26.858295601221254	26.328942898776077	23.245930235039797
66-67	23.613379121439475	26.85337616591565	26.21292982238355	23.320314890261322
68-69	23.65512303310636	26.91966506777876	26.109979520056985	23.315232379057896
70-71	23.69634338332682	26.99169025420945	25.972081074001196	23.339885288462536
72-73	23.704992084583264	27.037174646168754	25.835510132595218	23.42232313665276
74-75	23.789175359364172	27.0123207717904	25.819336484214983	23.37916738463045
76-77	23.280809179784466	27.42140173319792	25.57691698274661	23.720872104271002
78-79	23.42873415303595	27.39043801558442	25.467266426226086	23.71356140515354
80-81	23.44788947095228	27.473010770080375	25.389977713320732	23.689122045646613
82-83	23.460881542632745	27.62238524523002	25.259272040504165	23.657461171633066
84-85	23.432414660582044	27.6488880940314	25.079577001338894	23.839120244047667
86-87	23.354361048716985	27.727121046719986	25.042336213344907	23.87618169121812
88-89	23.314063116129958	27.82390612061102	24.949972071026583	23.91205869223244
90-91	23.313464418158773	27.827158673448892	24.76076619977476	24.09861070861757
92-93	23.267873481129254	27.89219873852002	24.65651401250144	24.183413767849288
94-95	23.22831434562479	27.97409193933173	24.48311733151676	24.314476383526724
96-97	23.164054061618625	28.040406809714163	24.331705185287166	24.463833943380042
98-99	23.12682180472396	28.084365164152608	24.194182778129587	24.594630252993845
100	23.7836021381909	28.138977869704707	23.526119437261077	24.551300554843316
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	217.0
1	159.0
2	105.5
3	111.0
4	116.5
5	136.5
6	189.0
7	261.5
8	328.5
9	416.0
10	609.0
11	974.0
12	1516.5
13	2394.0
14	3895.5
15	5962.0
16	8977.5
17	12052.0
18	14779.5
19	18516.0
20	23641.0
21	30164.0
22	38872.0
23	50388.0
24	63874.5
25	79373.5
26	99152.5
27	125118.5
28	154458.5
29	184486.0
30	214258.5
31	244213.0
32	278016.0
33	315196.0
34	353767.5
35	395802.0
36	441004.0
37	489376.0
38	536984.0
39	575314.0
40	605695.5
41	630709.5
42	650412.5
43	664344.5
44	669454.0
45	668169.0
46	663216.0
47	648875.0
48	630695.5
49	612869.0
50	591091.5
51	568864.5
52	545871.5
53	521063.5
54	496017.0
55	467425.5
56	435378.5
57	403438.5
58	374772.0
59	347743.0
60	317314.0
61	286894.5
62	259603.0
63	234697.5
64	209785.5
65	185833.5
66	163603.0
67	141503.5
68	120732.5
69	102945.0
70	87383.0
71	73891.5
72	63128.0
73	53957.0
74	45637.0
75	37934.0
76	31440.0
77	26446.0
78	22264.0
79	18514.5
80	15418.5
81	12674.0
82	9908.5
83	7528.0
84	5582.5
85	3913.0
86	2688.5
87	1861.5
88	1297.5
89	872.5
90	594.5
91	416.0
92	244.0
93	153.0
94	106.0
95	58.0
96	33.0
97	16.0
98	5.5
99	3.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1501994672049548
2	0.0
3	0.0015668853372220906
4	0.006807846637585635
5	0.02015879032129524
6	0.003593030169836863
7	6.159480291148908E-4
8	0.0037173003862372355
9	0.015031293131558123
10-11	5.673205531321363E-4
12-13	4.025274400794681E-4
14-15	0.00969307687922907
16-17	5.484098680277316E-4
18-19	8.239655652633408E-4
20-21	0.011573339283895579
22-23	0.007548064883100898
24-25	0.002569151647755531
26-27	5.457083415842454E-4
28-29	2.701526443486363E-5
30-31	0.002150415049015145
32-33	0.0051734231392763855
34-35	0.002009935673953854
36-37	9.104144114549042E-4
38-39	2.701526443486363E-4
40-41	0.0012481052168906998
42-43	8.050548801589362E-4
44-45	3.8631828141854995E-4
46-47	0.0014777349645870405
48-49	0.002833901239217195
50-51	0.0037064942804632905
52-53	1.4047937506129088E-4
54-55	0.0066970840534026944
56-57	0.0012913296399864817
58-59	0.006748413055828935
60-61	0.0026096745444078267
62-63	2.8095875012258177E-4
64-65	9.077128850114181E-4
66-67	0.0018235303493532953
68-69	0.0010319831014117906
70-71	0.006002791757426699
72-73	0.0010022663105334407
74-75	0.004614207165474708
76-77	0.0025637485948685587
78-79	0.00945534255220227
80-81	0.004384577417778367
82-83	1.8100227171358632E-4
84-85	0.0010535953129596814
86-87	0.0013993906977259362
88-89	0.002123399784580281
90-91	8.996083056809588E-4
92-93	0.0018883669839969677
94-95	0.003714598859793749
96-97	0.003938825554603118
98-99	0.01556889689381191
100	0.008477389979660207
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.8508055E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.98883921071551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.88858679581237	41.51125549658258
2	12.156872873113029	13.126709097032169
3	4.321250742304712	6.998979345464224
4	1.9589950995151897	4.230554857692208
5	1.0924298520411566	2.9489509815417874
6	0.6825985669967567	2.211162256743163
7	0.4747420593406229	1.7941540895813395
8	0.36393327034254214	1.5718667852762693
9	0.2722517008610686	1.3228697972368844
>10	1.6700446923598982	16.304201549007864
>50	0.07533021495081267	2.8288242726341304
>100	0.041204166948148026	3.949476948968262
>500	0.0014495742749739285	0.49093958160912843
>1k	2.1029964981716194E-4	0.2829421502790319
>5k	8.007319121302941E-5	0.29522167490044876
>10k+	2.001829780325643E-5	0.1318911154505602
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.403052886972726E-6	5.403052886972726E-6	0.0	0.0	5.403052886972726E-6
2	1.0806105773945453E-5	5.403052886972726E-6	0.0	0.0	5.403052886972726E-6
3	1.0806105773945453E-5	5.403052886972726E-6	0.0	0.0	5.403052886972726E-6
4	1.6209158660918178E-5	5.403052886972726E-6	0.0	0.0	5.403052886972726E-6
5	2.7015264434863632E-5	5.403052886972726E-6	0.0	0.0	1.0806105773945453E-5
6	2.7015264434863632E-5	5.403052886972726E-6	0.0	0.0	1.0806105773945453E-5
7	2.7015264434863632E-5	1.0806105773945453E-5	0.0	0.0	1.0806105773945453E-5
8	3.2418317321836356E-5	1.6209158660918178E-5	0.0	0.0	1.0806105773945453E-5
9	4.8627475982754534E-5	1.6209158660918178E-5	0.0	0.0	1.0806105773945453E-5
10-11	1.2156868995688634E-4	1.6209158660918178E-5	0.0	0.0	1.3507632217431816E-5
12-13	4.376472838447908E-4	1.8910685104404543E-5	0.0	0.0	1.6209158660918178E-5
14-15	0.0012372991111167543	2.1612211547890905E-5	0.0	0.0	1.6209158660918178E-5
16-17	0.0027420493401386584	2.7015264434863632E-5	0.0	0.0	1.6209158660918178E-5
18-19	0.00474658196120554	2.7015264434863632E-5	0.0	0.0	2.1612211547890905E-5
20-21	0.00750213893356163	2.7015264434863632E-5	0.0	0.0	2.1612211547890905E-5
22-23	0.01073316455997132	2.7015264434863632E-5	0.0	0.0	2.1612211547890905E-5
24-25	0.014707109958339761	2.9716790878349994E-5	0.0	0.0	2.1612211547890905E-5
26-27	0.018737787412021416	3.7821370208809086E-5	0.0	0.0	2.1612211547890905E-5
28-29	0.022817092341685823	3.7821370208809086E-5	2.701526443486363E-6	0.0	2.1612211547890905E-5
30-31	0.02665866294432343	4.322442309578181E-5	5.403052886972726E-6	0.0	2.1612211547890905E-5
32-33	0.029908599255837526	4.322442309578181E-5	5.403052886972726E-6	0.0	2.1612211547890905E-5
34-35	0.03274250049505472	4.322442309578181E-5	5.403052886972726E-6	0.0	2.1612211547890905E-5
36-37	0.0356979704242288	4.322442309578181E-5	5.403052886972726E-6	0.0	2.1612211547890905E-5
38-39	0.03858049913942875	4.592594953926817E-5	5.403052886972726E-6	0.0	2.1612211547890905E-5
40-41	0.04139819121998503	5.4030528869727264E-5	1.0806105773945453E-5	5.403052886972726E-6	2.4313737991377267E-5
42-43	0.04434015351694168	5.943358175669999E-5	1.0806105773945453E-5	5.403052886972726E-6	3.2418317321836356E-5
44-45	0.04753065624669907	5.943358175669999E-5	1.0806105773945453E-5	5.403052886972726E-6	3.2418317321836356E-5
46-47	0.05121553831561447	5.943358175669999E-5	1.0806105773945453E-5	5.403052886972726E-6	3.2418317321836356E-5
48-49	0.05518948371398291	6.213510820018635E-5	1.0806105773945453E-5	5.403052886972726E-6	3.7821370208809086E-5
50-51	0.059828004617449	6.483663464367271E-5	1.0806105773945453E-5	8.104579330459089E-6	4.322442309578181E-5
52-53	0.06542826893479622	6.753816108715908E-5	1.0806105773945453E-5	1.0806105773945453E-5	4.322442309578181E-5
54-55	0.07249005905806957	7.023968753064544E-5	1.0806105773945453E-5	1.6209158660918178E-5	5.673205531321363E-5
56-57	0.08087829866509474	7.294121397413181E-5	1.0806105773945453E-5	1.6209158660918178E-5	6.483663464367271E-5
58-59	0.0912089357849866	8.644884619156362E-5	1.0806105773945453E-5	2.1612211547890905E-5	6.483663464367271E-5
60-61	0.10387099022560717	8.644884619156362E-5	1.0806105773945453E-5	2.1612211547890905E-5	6.753816108715908E-5
62-63	0.11942637948720165	8.644884619156362E-5	1.0806105773945453E-5	2.9716790878349994E-5	7.023968753064544E-5
64-65	0.13840730427913683	8.915037263504999E-5	1.0806105773945453E-5	3.2418317321836356E-5	7.294121397413181E-5
66-67	0.16130544241412725	9.455342552202272E-5	1.0806105773945453E-5	3.511984376532272E-5	8.104579330459089E-5
68-69	0.18933377926529826	1.026580048524818E-4	1.0806105773945453E-5	3.7821370208809086E-5	8.104579330459089E-5
70-71	0.22384848110728006	1.1616563706991361E-4	1.0806105773945453E-5	3.7821370208809086E-5	8.104579330459089E-5
72-73	0.26527098606525645	1.1886716351339998E-4	1.0806105773945453E-5	3.7821370208809086E-5	9.185189907853635E-5
74-75	0.3143712291756211	1.242702164003727E-4	1.0806105773945453E-5	3.7821370208809086E-5	9.185189907853635E-5
76-77	0.37295923315550983	1.3507632217431815E-4	1.0806105773945453E-5	4.052289665229545E-5	9.725495196550907E-5
78-79	0.44037852707915554	1.377778486178045E-4	1.0806105773945453E-5	4.8627475982754534E-5	1.0535953129596816E-4
80-81	0.519433295394897	1.4588242794826361E-4	1.0806105773945453E-5	5.4030528869727264E-5	1.0806105773945453E-4
82-83	0.6093482000134536	1.5128548083523635E-4	1.0806105773945453E-5	5.673205531321363E-5	1.0806105773945453E-4
84-85	0.7125573162604066	1.5668853372220905E-4	1.0806105773945453E-5	6.753816108715908E-5	1.0806105773945453E-4
86-87	0.8313326278747281	1.6209158660918178E-4	1.3507632217431816E-5	7.564274041761817E-5	1.0806105773945453E-4
88	0.927515073842173	1.674946394961545E-4	1.6209158660918178E-5	8.104579330459089E-5	1.0806105773945453E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	12745	0.0	40.74184	1
TCTCGAG	14610	0.0	35.38931	2
CTCGAGG	19120	0.0	27.094921	3
GATCCGG	18845	0.0	26.304932	1
TCGAGGG	22855	0.0	22.687595	4
ATCCGGC	21965	0.0	22.426407	2
TATCTCG	6875	0.0	21.501162	1
TCCGGCG	24840	0.0	19.871557	3
CGAGGGC	29375	0.0	17.523888	5
GCGCCGT	28790	0.0	17.077501	9
GGATCCG	12720	0.0	16.887241	2
ATATCTC	9245	0.0	16.498447	1
CGGCGCG	31920	0.0	15.31668	5
AGGGCGC	33780	0.0	15.291625	7
CGTCGAC	26945	0.0	15.114739	12-13
GAGGGCG	35310	0.0	14.602684	6
CCGGCGC	34050	0.0	14.579445	4
GCCGTCG	28365	0.0	14.465717	10-11
TCGACTT	28885	0.0	14.158167	14-15
TATGGAT	11545	0.0	14.108704	1
>>END_MODULE