FastQCFastQC Report
Wed 25 May 2016
SRR491026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27784567
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA326630.11755806739763121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA168700.036.948791
TCTCGAG185300.033.382182
GATCCGG254150.025.4150711
CTCGAGG250700.024.8273943
TATCTCG87500.024.2271672
ATCCGGC289100.022.2744312
TCGAGGG286500.021.6758274
TCCGGCG334700.019.326813
GCGCCGT361400.017.636299
ATATCTC141150.015.9765111
CGAGGGC396500.015.6623565
CGGCGCG419200.015.28523355
CGTCGAC336000.015.12870912-13
GGATCCG176500.014.966692
GCCGTCG354400.014.55536610-11
TATGGAT160700.014.4784972
AGGGCGC441250.014.444722
CCGGCGC453100.014.2557534
TCGACTT367100.013.95767314-15
GAGGGCG470100.013.7100961
Produced by FastQC (version 0.11.5)