##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491026_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27784567
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.692030039553973	34.0	31.0	34.0	29.0	34.0
2	31.934311411079396	34.0	31.0	34.0	30.0	34.0
3	31.99476936243059	34.0	31.0	34.0	30.0	34.0
4	35.356343829291994	37.0	35.0	37.0	33.0	37.0
5	35.135408588516064	37.0	35.0	37.0	32.0	37.0
6	35.071998134791876	37.0	35.0	37.0	32.0	37.0
7	34.993406231596126	37.0	35.0	37.0	32.0	37.0
8	34.9106200935217	37.0	35.0	37.0	32.0	37.0
9	36.44008585773534	39.0	37.0	39.0	32.0	39.0
10-11	36.271877027991835	39.0	37.0	39.0	32.0	39.0
12-13	36.121173527735735	39.0	37.0	39.0	32.0	39.0
14-15	37.410702531372905	40.0	38.0	41.0	32.0	41.0
16-17	37.2810046310961	40.0	38.0	41.0	31.0	41.0
18-19	37.11753400727821	40.0	38.0	41.0	31.0	41.0
20-21	36.95442493309325	40.0	38.0	41.0	30.5	41.0
22-23	36.74206544230111	40.0	37.5	41.0	30.0	41.0
24-25	36.524710282510426	40.0	37.0	41.0	29.5	41.0
26-27	36.16532492660404	40.0	36.5	41.0	27.5	41.0
28-29	35.84344800118713	40.0	36.0	41.0	27.0	41.0
30-31	35.541440883350816	39.5	35.5	41.0	25.5	41.0
32-33	35.30488851958715	39.0	35.0	41.0	25.0	41.0
34-35	35.08160335915978	39.0	35.0	41.0	24.0	41.0
36-37	34.75938242982156	39.0	35.0	40.5	22.0	41.0
38-39	34.414993456619285	39.0	35.0	40.0	19.0	41.0
40-41	33.95175778697577	38.5	34.0	40.0	14.5	41.0
42-43	33.556088727242	38.0	33.5	40.0	8.5	41.0
44-45	33.096095415127394	38.0	33.0	40.0	4.0	41.0
46-47	32.549464960170155	38.0	32.5	40.0	2.0	41.0
48-49	32.01976512716574	38.0	31.5	40.0	2.0	41.0
50-51	31.55736511927647	37.5	31.0	40.0	2.0	41.0
52-53	30.995737525799843	37.0	30.0	40.0	2.0	41.0
54-55	30.464210653345795	36.5	29.0	39.5	2.0	41.0
56-57	29.943874741686635	36.0	28.0	39.0	2.0	40.0
58-59	29.397083873936204	35.5	27.0	39.0	2.0	40.0
60-61	28.81934287476929	35.0	26.0	38.5	2.0	40.0
62-63	28.181174912677243	34.5	24.5	38.0	2.0	40.0
64-65	27.533629514543094	34.0	22.5	38.0	2.0	40.0
66-67	26.909154603704998	34.0	20.5	37.5	2.0	39.5
68-69	26.178741547420913	33.0	15.5	36.5	2.0	39.0
70-71	25.407703942264064	32.0	8.5	36.0	2.0	39.0
72-73	24.66020147083811	31.5	4.5	35.5	2.0	38.5
74-75	23.854784744351065	31.0	2.0	35.0	2.0	37.5
76-77	22.867232877877854	29.5	2.0	34.0	2.0	36.0
78-79	22.850524015724268	30.0	2.0	34.0	2.0	36.0
80-81	22.401573686572117	30.0	2.0	34.0	2.0	36.0
82-83	21.82816565037706	29.0	2.0	34.0	2.0	35.5
84-85	21.024969041266687	29.0	2.0	34.0	2.0	35.0
86-87	20.298124458804775	27.5	2.0	34.0	2.0	35.0
88-89	19.646714091315516	26.5	2.0	33.0	2.0	35.0
90-91	18.804511007855545	25.5	2.0	33.0	2.0	34.5
92-93	17.99675485315283	24.5	2.0	32.0	2.0	34.0
94-95	17.051409528894226	19.5	2.0	32.0	2.0	34.0
96-97	16.057161391070085	9.0	2.0	31.0	2.0	34.0
98-99	15.109684181869742	2.0	2.0	31.0	2.0	34.0
100	14.2820958123983	2.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15077300713107533
1101	2	0.19550532842108126
1101	3	0.15468153576802735
1101	4	0.1699125343477661
1101	5	0.18960044414515664
1101	6	0.2116044356958824
1101	7	0.2215255055649621
1101	8	0.2303352405988548
1101	9	0.24885939464388684
1101	10-11	0.26216120315451263
1101	12-13	0.2663958171565426
1101	14-15	0.27574281497667386
1101	16-17	0.2847807583352875
1101	18-19	0.2989062124684949
1101	20-21	0.27569339054440434
1101	22-23	0.25218776304035373
1101	24-25	0.24638708618086014
1101	26-27	0.2511391645118053
1101	28-29	0.21887213327069333
1101	30-31	0.223471027736295
1101	32-33	0.18793159879576393
1101	34-35	0.19243144636135412
1101	36-37	0.163327583600676
1101	38-39	0.08630447006682829
1101	40-41	0.1239078937012863
1101	42-43	-0.04420119865042693
1101	44-45	-0.10176151592065708
1101	46-47	-0.05635902864401743
1101	48-49	-0.017185505770726195
1101	50-51	-0.11707468539536592
1101	52-53	-0.29285422320591437
1101	54-55	-0.3278572698586437
1101	56-57	-0.3297651213148107
1101	58-59	-0.4202254505194567
1101	60-61	-0.5634454956045154
1101	62-63	-0.6709243574324049
1101	64-65	-0.6256533174043213
1101	66-67	-0.609914926669127
1101	68-69	-0.6922122554322243
1101	70-71	-0.6616591638638845
1101	72-73	-0.7555978743150362
1101	74-75	-0.7520162331108295
1101	76-77	-0.6354238798382141
1101	78-79	-0.7222178477945072
1101	80-81	-0.8134737177876588
1101	82-83	-0.7951021406953451
1101	84-85	-0.8497640816891163
1101	86-87	-0.9570108522271781
1101	88-89	-0.8133193581020812
1101	90-91	-0.7602356578650848
1101	92-93	-0.7559403022236708
1101	94-95	-0.796653250399384
1101	96-97	-0.7434166406486202
1101	98-99	-0.7739809428837958
1101	100	-0.8545841319362832
1102	1	0.15845136942269988
1102	2	0.16875726028466076
1102	3	0.16028058280856072
1102	4	0.11446265002608413
1102	5	0.16389377162887087
1102	6	0.1583228918243904
1102	7	0.16762579750927387
1102	8	0.16249113410085414
1102	9	0.15603285596414906
1102	10-11	0.16538387719582204
1102	12-13	0.18471474982989378
1102	14-15	0.20829479221340108
1102	16-17	0.21060653081248404
1102	18-19	0.25719847815584274
1102	20-21	0.1841678858195408
1102	22-23	0.22178824367814087
1102	24-25	0.24637956222375834
1102	26-27	0.23702067950960526
1102	28-29	0.19128547949544839
1102	30-31	0.16001617237115795
1102	32-33	0.1682019478145591
1102	34-35	0.08061115462855639
1102	36-37	0.14958015668166524
1102	38-39	0.1133997388736745
1102	40-41	0.16528161209468806
1102	42-43	0.06763236076618284
1102	44-45	0.0296934359158314
1102	46-47	0.13063217271287897
1102	48-49	0.161218320742897
1102	50-51	0.05822256658615288
1102	52-53	0.07406173272509875
1102	54-55	0.04401044110379004
1102	56-57	0.05330654356110287
1102	58-59	-0.051924464914488766
1102	60-61	-0.12015600790077485
1102	62-63	-0.12873694464954255
1102	64-65	-0.1161405307205996
1102	66-67	-0.14105325549370562
1102	68-69	-0.1868831609822017
1102	70-71	-0.21826413129167932
1102	72-73	-0.23768734092509192
1102	74-75	-0.1479781009678014
1102	76-77	-0.12473414370302294
1102	78-79	-0.09124102743519913
1102	80-81	-0.2055918919371038
1102	82-83	-0.12097408097083218
1102	84-85	-0.13314829281344487
1102	86-87	-0.1817869166001529
1102	88-89	-0.08708478829900201
1102	90-91	0.028174035580665446
1102	92-93	0.05820895431131845
1102	94-95	0.01970471751208791
1102	96-97	0.015806468471350144
1102	98-99	0.12583556305356325
1102	100	0.11612631730517542
1103	1	0.06580288202556517
1103	2	0.08378135397451203
1103	3	0.08277705388678669
1103	4	0.07491783907218519
1103	5	0.12768918935674378
1103	6	0.11630042009765873
1103	7	0.0915009827423674
1103	8	0.05160772890695853
1103	9	0.112170952886963
1103	10-11	0.09140169563079326
1103	12-13	0.11025465654108757
1103	14-15	0.13482476221522433
1103	16-17	0.1624040778102227
1103	18-19	0.21409070039209865
1103	20-21	0.18266702815817126
1103	22-23	0.1694169787919506
1103	24-25	0.15078764202797146
1103	26-27	0.23566138979253992
1103	28-29	0.23249826790865313
1103	30-31	0.23305919171748712
1103	32-33	0.10886440245551654
1103	34-35	0.08788354615130345
1103	36-37	0.09328780573808615
1103	38-39	0.16708326089752035
1103	40-41	0.18819750071079255
1103	42-43	0.07861351567382258
1103	44-45	-0.0286637238313574
1103	46-47	0.07813846635414734
1103	48-49	-0.06524964846878234
1103	50-51	0.05716188028028313
1103	52-53	-0.04279823781533665
1103	54-55	-0.0550929908065072
1103	56-57	0.0058961745340688765
1103	58-59	-0.04076564257984572
1103	60-61	-0.14908594939807784
1103	62-63	2.372406124955262E-4
1103	64-65	0.028786613075542533
1103	66-67	0.0064185020884863775
1103	68-69	-0.07336823079137744
1103	70-71	-0.08060349890964957
1103	72-73	-0.08442444543514682
1103	74-75	-0.06788264613592787
1103	76-77	-0.07780007590949012
1103	78-79	-0.07294374769687195
1103	80-81	-0.07105647051456287
1103	82-83	-0.03537491584383545
1103	84-85	-0.2338761880896847
1103	86-87	-0.11217965513463213
1103	88-89	0.047639770630862444
1103	90-91	0.23123465275473265
1103	92-93	0.3428524027427322
1103	94-95	0.1673820145493501
1103	96-97	0.20378914696082617
1103	98-99	0.16673991840028712
1103	100	0.2517386409252662
1104	1	-0.01469937552194267
1104	2	0.006896046876246231
1104	3	-0.013198762920239915
1104	4	-0.006645366726225177
1104	5	-0.021492733117561613
1104	6	-0.023524533981579054
1104	7	-0.025526021334862037
1104	8	-0.014983175915567415
1104	9	-0.06811902708737705
1104	10-11	-0.04549343570326414
1104	12-13	0.012306752703359791
1104	14-15	0.03927566844082975
1104	16-17	0.08468674716129243
1104	18-19	0.05421717286490946
1104	20-21	0.05509890844067655
1104	22-23	0.044433857026056955
1104	24-25	0.09295588409693067
1104	26-27	0.15762350958329563
1104	28-29	0.01591547454388831
1104	30-31	0.07515830495592013
1104	32-33	0.09139304459421993
1104	34-35	0.08299761795246496
1104	36-37	0.1236554097362017
1104	38-39	0.15278070878147076
1104	40-41	0.2459872357825077
1104	42-43	0.17613527298162523
1104	44-45	0.11462087922055275
1104	46-47	0.2057116177512981
1104	48-49	0.24168211958711794
1104	50-51	0.22791786364645716
1104	52-53	0.16618924423139703
1104	54-55	0.19091966235927416
1104	56-57	0.19219569548151227
1104	58-59	0.18836838408313739
1104	60-61	0.16720835504029097
1104	62-63	0.16162155574832582
1104	64-65	0.1923090013623252
1104	66-67	0.16813005348523902
1104	68-69	0.1686778762975969
1104	70-71	0.1957367794357303
1104	72-73	0.16940736203633833
1104	74-75	0.3479787791990461
1104	76-77	0.3409091789253367
1104	78-79	0.28931580019089154
1104	80-81	0.2426749825584693
1104	82-83	0.32307510298697295
1104	84-85	0.32720293258347866
1104	86-87	0.2832812751570444
1104	88-89	0.4122453939031594
1104	90-91	0.4966856336833949
1104	92-93	0.4362047403936984
1104	94-95	0.4257023183592956
1104	96-97	0.5535386386287904
1104	98-99	0.5271932709334823
1104	100	0.6181843846705846
1105	1	-0.06105556253566391
1105	2	-0.06524821824344684
1105	3	-0.11606630668230977
1105	4	-0.07256160742970508
1105	5	-0.06663104415546428
1105	6	-0.09853130718389735
1105	7	-0.11003799600043607
1105	8	-0.053334020652357594
1105	9	-0.08568998232025393
1105	10-11	-0.09561985536318218
1105	12-13	-0.10828745217352775
1105	14-15	-0.07483864031890874
1105	16-17	-0.09975807873378528
1105	18-19	-0.034479878868694414
1105	20-21	-0.04173503813969859
1105	22-23	-0.054405675344774806
1105	24-25	-0.09067855424472526
1105	26-27	-0.2060595633499105
1105	28-29	-0.15367271435171403
1105	30-31	-0.013901645331117152
1105	32-33	-0.046398252566710596
1105	34-35	-0.03252845573566532
1105	36-37	0.0031990020465357816
1105	38-39	0.029728134023265795
1105	40-41	0.09029179577350988
1105	42-43	0.05743852417236894
1105	44-45	0.003504211589650197
1105	46-47	0.11372212267999515
1105	48-49	0.10532360885620307
1105	50-51	0.10452177323614364
1105	52-53	0.05441742810558736
1105	54-55	0.07264880196085599
1105	56-57	0.10427609568255747
1105	58-59	0.1261012815240612
1105	60-61	0.14425897012416655
1105	62-63	0.167958511550669
1105	64-65	0.19369480647026194
1105	66-67	0.1625692174168769
1105	68-69	0.16482015470846534
1105	70-71	0.199431446172202
1105	72-73	0.21304719997534605
1105	74-75	0.3938021606615436
1105	76-77	0.42224862243507033
1105	78-79	0.3339445720707275
1105	80-81	0.3048654893718066
1105	82-83	0.30778233570649505
1105	84-85	0.33771887410137325
1105	86-87	0.3692347736536874
1105	88-89	0.4843872491519079
1105	90-91	0.47668056093401745
1105	92-93	0.4101544057995632
1105	94-95	0.5355064422865965
1105	96-97	0.6601183814607197
1105	98-99	0.618332544545261
1105	100	0.7355862255227663
1106	1	-0.2155007406963243
1106	2	-0.19196762968512004
1106	3	-0.2050219086628502
1106	4	-0.21066341420218038
1106	5	-0.25058355278735434
1106	6	-0.2574398716532045
1106	7	-0.23930886114276007
1106	8	-0.2292225735047282
1106	9	-0.2708890720470123
1106	10-11	-0.2823413830714827
1106	12-13	-0.275439377529338
1106	14-15	-0.34067740291452253
1106	16-17	-0.4347554961308333
1106	18-19	-0.5800338782336212
1106	20-21	-0.5185429705092588
1106	22-23	-0.5535887405264717
1106	24-25	-0.6855568795279012
1106	26-27	-0.7857318203805761
1106	28-29	-0.6060414286837954
1106	30-31	-0.729602375311373
1106	32-33	-0.6865002186799742
1106	34-35	-0.5202030828977442
1106	36-37	-0.5486940398445057
1106	38-39	-0.4632996347446863
1106	40-41	-0.5800160459497476
1106	42-43	-0.6852147887441049
1106	44-45	-0.6354315718162127
1106	46-47	-0.5319690909140249
1106	48-49	-0.5532923536804617
1106	50-51	-0.6005075693408344
1106	52-53	-0.717708608066431
1106	54-55	-0.654676842466241
1106	56-57	-0.43411912398959274
1106	58-59	-0.4936469137570789
1106	60-61	-0.44571231355739727
1106	62-63	-0.49374850876594323
1106	64-65	-0.4988542634173214
1106	66-67	-0.5457262013149737
1106	68-69	-0.5646806168624678
1106	70-71	-0.5938676352810468
1106	72-73	-0.5613104696943481
1106	74-75	-0.2861649055977722
1106	76-77	-0.36120037893246604
1106	78-79	-0.4738006373254855
1106	80-81	-0.49822250350995034
1106	82-83	-0.41681166710871054
1106	84-85	-0.4119051304816388
1106	86-87	-0.3567618117874858
1106	88-89	-0.15880261626999115
1106	90-91	-0.2700021201209353
1106	92-93	-0.23958700276556755
1106	94-95	-0.24066121222906744
1106	96-97	-0.22046598091271008
1106	98-99	-0.31327399074795714
1106	100	-0.2233548333478037
1107	1	-0.2787535987254408
1107	2	-0.2067252639784627
1107	3	-0.2600978220306551
1107	4	-0.2023301727639577
1107	5	-0.2098190757583538
1107	6	-0.24974069056485604
1107	7	-0.241107088503135
1107	8	-0.25283811350605134
1107	9	-0.2643896557082286
1107	10-11	-0.2744093028821979
1107	12-13	-0.2895457917909141
1107	14-15	-0.27026345869264645
1107	16-17	-0.2796746754678878
1107	18-19	-0.2709609614109354
1107	20-21	-0.24830903420756556
1107	22-23	-0.35534904757577834
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2207	88-89	0.755208810111359
2207	90-91	0.7263465127413049
2207	92-93	0.8670696859887066
2207	94-95	0.9597560954203033
2207	96-97	0.916181761238338
2207	98-99	0.9497276649283553
2207	100	0.893704936509419
2208	1	-0.8393704686024925
2208	2	-0.6677776358807108
2208	3	-0.7156740917905431
2208	4	-0.6471144261214192
2208	5	-0.744723411499983
2208	6	-0.7328449668375185
2208	7	-0.7557057021250273
2208	8	-0.753312679883642
2208	9	-0.8438192295215288
2208	10-11	-0.9625528154349325
2208	12-13	-0.989629765361542
2208	14-15	-1.1062295458826341
2208	16-17	-1.0110005924890046
2208	18-19	-1.0334997251872835
2208	20-21	-1.1771415705684305
2208	22-23	-1.0118150241363466
2208	24-25	-1.0957932316285621
2208	26-27	-1.4173877519783034
2208	28-29	-1.336196629030482
2208	30-31	-1.3415194716708427
2208	32-33	-1.4147936723822099
2208	34-35	-1.0873980222449404
2208	36-37	-1.4455009219790043
2208	38-39	-1.4523578505616825
2208	40-41	-2.046523526958058
2208	42-43	-1.2384540853423829
2208	44-45	-0.9504308679683646
2208	46-47	-1.5341665726318467
2208	48-49	-1.6493950287802086
2208	50-51	-1.5789884741077316
2208	52-53	-0.9222708667174402
2208	54-55	-0.7717648440580156
2208	56-57	-0.6600367355355559
2208	58-59	-0.803965492841872
2208	60-61	-0.6890697778801069
2208	62-63	-0.8597191382450013
2208	64-65	-0.8860182081579673
2208	66-67	-0.7974215407051268
2208	68-69	-1.2034381090737902
2208	70-71	-1.208429010842032
2208	72-73	-0.9438824087210165
2208	74-75	-1.7986226185480056
2208	76-77	-1.2981844791134733
2208	78-79	-1.6792664032609501
2208	80-81	-1.6351942854769703
2208	82-83	-1.5861981456545458
2208	84-85	-2.200782790951525
2208	86-87	-1.9993420135845739
2208	88-89	-2.1268393689198994
2208	90-91	-2.188584540222937
2208	92-93	-2.8494926652992945
2208	94-95	-2.9678707058853817
2208	96-97	-2.952478831056343
2208	98-99	-3.0037496821485163
2208	100	-3.052766366144711
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	413673.0
3	181543.0
4	177138.0
5	148575.0
6	138107.0
7	139092.0
8	143581.0
9	158798.0
10	182726.0
11	205603.0
12	227943.0
13	262367.0
14	311118.0
15	382404.0
16	447710.0
17	459637.0
18	436214.0
19	423402.0
20	429961.0
21	448301.0
22	468080.0
23	489868.0
24	516226.0
25	546914.0
26	585680.0
27	635957.0
28	705405.0
29	796243.0
30	911649.0
31	1054879.0
32	1242944.0
33	1510704.0
34	1943823.0
35	2647226.0
36	3495593.0
37	3298049.0
38	1174893.0
39	42531.0
40	10.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.094181695633807	28.025762779853448	27.414262098611886	23.465793425900863
2	24.264506263495125	28.135097444563378	27.01622810965526	20.58416818228623
3	23.28841448818204	26.24505441186215	26.922740399754435	23.543790700201377
4	23.822852942912633	26.011972207144886	26.621852175528062	23.54332267441442
5	24.22747575320392	26.274041020365324	26.58178753607352	22.916695690357233
6	24.277312344411055	26.008606893036866	25.920133661849125	23.793947100702955
7	24.392035135428806	26.382768992804838	26.039594121545356	23.185601750220997
8	23.584221020798623	26.202197338487082	26.56753620248492	23.646045438229375
9	23.765788444366628	25.81271841477802	26.6426198779955	23.778873262859857
10-11	23.741711357206007	26.15893254359532	26.351054714004302	23.74830138519437
12-13	23.56512788707541	26.202257316308202	26.465606026766007	23.767008769850374
14-15	23.332435507346432	26.388012219744034	26.84830431470477	23.431247958204768
16-17	23.205503330131478	26.32257110627871	26.9499300390127	23.521995524577115
18-19	22.91264064436848	26.23723872477712	27.45482519998398	23.39529543087042
20-21	22.7236876620639	26.04087373560351	28.09096230691252	23.144476295420073
22-23	22.680020667734848	26.052183690882387	28.36592976821506	22.901865873167704
24-25	22.676587819869056	25.888409701612265	28.642832635967828	22.79216984255085
26-27	22.718515282624747	25.926214923565503	28.679066005084685	22.676203788725065
28-29	22.744520944773008	25.99038692626291	28.63203058029627	22.633061548667815
30-31	22.743696142068657	25.921890751299177	28.56571149242322	22.768701614208954
32-33	22.77712739392493	25.962763314353076	28.548181451821968	22.711927839900028
34-35	22.834537991167263	26.0541338665749	28.473196140495165	22.638132001762667
36-37	22.86113822271212	25.976273176099884	28.39822073834845	22.764367862839542
38-39	22.88764208643554	26.023889366213442	28.34111333169405	22.74735521565696
40-41	22.976583978139697	26.140906003727903	28.111889883783242	22.77062013434916
42-43	23.092891268216253	26.218207656166392	27.736436119030145	22.95246495658721
44-45	23.196322275179682	26.356252265542462	27.434695015942456	23.012730443335403
46-47	23.332317107242158	26.539445309715283	27.054571341096135	23.073666241946427
48-49	23.453720964307987	26.516353300967445	26.77309123427738	23.256834500447184
50-51	23.533189076413848	26.556157916786066	26.592433002982364	23.31822000381773
52-53	23.590243978986287	26.64728033490874	26.46709369575667	23.295381990348297
54-55	23.619900495721712	26.554040325418043	26.403201989725627	23.422857189134618
56-57	23.634590787810637	26.61773379331091	26.389536187998285	23.358139230880166
58-59	23.663620740431035	26.66777455106746	26.342607959879466	23.32599674862204
60-61	23.657512588458435	26.620701768372363	26.24306736108605	23.478718282083147
62-63	23.654902506158745	26.702007268592066	26.23404985943048	23.409040365818708
64-65	23.698799270098807	26.798136797250145	26.1022709994744	23.400792933176653
66-67	23.738474025708175	26.772532482182804	25.99694691623225	23.492046575876774
68-69	23.765585029661562	26.8549116361683	25.91520383382327	23.46429950034687
70-71	23.797854776836587	26.92286816185056	25.78192687115837	23.49735019015448
72-73	23.80155648283353	26.94193330487363	25.654096706166058	23.602413506126783
74-75	23.888907165241076	26.94872305046891	25.639138833002047	23.52323095128797
76-77	23.385400151526873	27.329027474594053	25.4252956303752	23.86027674350387
78-79	23.52518048666903	27.291296262877925	25.301596098567703	23.88192715188535
80-81	23.524860692282893	27.377181689728573	25.262650396860643	23.835307221127895
82-83	23.552710181979762	27.517099630808783	25.101200243963202	23.82898994324825
84-85	23.504229553084052	27.551348794104847	24.934055877598727	24.010365775212374
86-87	23.413028301099896	27.637781481125895	24.903310491487645	24.04587972628656
88-89	23.3796317208296	27.747818463857783	24.803898664327285	24.068651150985332
90-91	23.367549311998197	27.736917328443866	24.627584709277855	24.267948650280086
92-93	23.321313726495145	27.798481704321848	24.55525019248455	24.324954376698457
94-95	23.29551363660896	27.89251988187178	24.358344420384036	24.45362206113523
96-97	23.209199055178942	27.968103830976858	24.205608357950496	24.617088755893704
98-99	23.17599368254804	28.022175876242738	24.078068227581255	24.723762213627968
100	23.84379819063539	28.071596389333486	23.397520264655416	24.68708515537571
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3828.0
1	2826.0
2	1562.0
3	1317.5
4	1377.5
5	1564.5
6	1780.0
7	2028.5
8	2415.0
9	2867.5
10	3459.0
11	4390.5
12	5648.0
13	7451.5
14	11378.0
15	16137.0
16	22414.5
17	26164.0
18	27705.5
19	33044.5
20	43690.0
21	54542.0
22	65557.0
23	82258.5
24	102325.0
25	126149.0
26	155358.0
27	193122.0
28	237259.0
29	280688.5
30	321924.0
31	364459.5
32	410581.0
33	460004.0
34	514114.0
35	571842.5
36	632953.5
37	698527.0
38	761137.0
39	815222.0
40	859613.5
41	895052.5
42	922068.5
43	942846.5
44	952994.0
45	954559.5
46	953815.0
47	942138.5
48	924279.5
49	904805.5
50	878960.0
51	853448.5
52	825519.0
53	794947.0
54	765757.5
55	731891.5
56	689891.0
57	642638.5
58	599854.5
59	559589.0
60	512889.0
61	466182.5
62	423552.0
63	382517.0
64	343676.5
65	304936.0
66	266503.5
67	229980.0
68	196332.5
69	166797.5
70	141557.5
71	119445.5
72	101123.0
73	85979.0
74	72453.5
75	60078.0
76	49387.0
77	41026.0
78	34197.0
79	28424.0
80	23101.0
81	18305.5
82	14408.0
83	11089.5
84	8187.0
85	5838.5
86	4117.5
87	2812.5
88	1925.5
89	1309.0
90	897.0
91	643.0
92	438.0
93	259.5
94	158.0
95	99.5
96	61.0
97	32.5
98	14.0
99	5.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1558635050889942
2	0.0
3	0.0016555953526286734
4	0.006791540066109362
5	0.019924730156852904
6	0.003678300979101096
7	6.838328630422782E-4
8	0.0037646798670643313
9	0.015407834140442066
10-11	5.866566140836386E-4
12-13	4.0850015765946617E-4
14-15	0.00951247503695127
16-17	5.524649709315248E-4
18-19	7.774099916691161E-4
20-21	0.0118033151281429
22-23	0.007673324547400721
24-25	0.0030034659168883216
26-27	5.560640912633261E-4
28-29	3.41916431521139E-5
30-31	0.002334029535173249
32-33	0.005044167145019751
34-35	0.0019309280580114855
36-37	8.38595037309741E-4
38-39	2.5553754355790394E-4
40-41	0.0011571171866741705
42-43	8.781853609595571E-4
44-45	3.833063153368559E-4
46-47	0.0014234520912274789
48-49	0.0028361068214595533
50-51	0.0037268891035804157
52-53	1.5476217426746295E-4
54-55	0.006629579651178296
56-57	0.0011301237841856596
58-59	0.00678434182544576
60-61	0.002463597867118102
62-63	3.3831731118933757E-4
64-65	9.303726057706785E-4
66-67	0.0018985359750252721
68-69	0.0010509431368860274
70-71	0.005866566140836386
72-73	9.285730456047777E-4
74-75	0.004480904813092822
76-77	0.002580569277901649
78-79	0.009600653485080404
80-81	0.004317144837995856
82-83	1.7635689625827172E-4
84-85	8.943814024526637E-4
86-87	0.0014378485725546847
88-89	0.0021234809957628637
90-91	8.223989958166345E-4
92-93	0.0018679434522049596
94-95	0.0037016952612578054
96-97	0.0039770279666406175
98-99	0.01576234749312451
100	0.008378752132433807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.7784567E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.554903729510144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64259564657415	37.7354923703331
2	12.972764914686893	13.116737627351258
3	4.890697586200467	7.417462369215366
4	2.2957328732234386	4.642422175779306
5	1.2660474504821688	3.2002453488058906
6	0.8233309605943255	2.497405047022274
7	0.5313734516195383	1.8804473583730295
8	0.37737976115038907	1.5262717995538762
9	0.28585700338500425	1.300632595788163
>10	1.7468014359496533	17.165070994911748
>50	0.1226512059870059	4.155594326749535
>100	0.04216934705555586	3.789122314761112
>500	0.002275520535197852	0.7462547281372971
>1k	2.0171921280722843E-4	0.16322515132570004
>5k	7.837392807714435E-5	0.28496404700385897
>10k+	4.2749415276087945E-5	0.37865174488833236
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32663	0.11755806739763121	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0797360995404392E-5	0.0
2	3.5991203318014636E-6	0.0	0.0	1.4396481327205854E-5	0.0
3	3.5991203318014636E-6	3.5991203318014636E-6	0.0	1.799560165900732E-5	3.5991203318014636E-6
4	3.5991203318014636E-6	3.5991203318014636E-6	0.0	1.799560165900732E-5	3.5991203318014636E-6
5	1.4396481327205854E-5	3.5991203318014636E-6	0.0	1.799560165900732E-5	3.5991203318014636E-6
6	2.1594721990808783E-5	3.5991203318014636E-6	0.0	1.799560165900732E-5	3.5991203318014636E-6
7	2.1594721990808783E-5	7.198240663602927E-6	0.0	1.799560165900732E-5	3.5991203318014636E-6
8	2.879296265441171E-5	7.198240663602927E-6	0.0	2.1594721990808783E-5	3.5991203318014636E-6
9	4.3189443981617566E-5	7.198240663602927E-6	0.0	2.5193842322610246E-5	7.198240663602927E-6
10-11	1.295683319448527E-4	1.2596921161305123E-5	0.0	3.95903236498161E-5	7.198240663602927E-6
12-13	3.995023568299624E-4	1.799560165900732E-5	0.0	6.298460580652562E-5	7.198240663602927E-6
14-15	0.001052742697051928	1.979516182490805E-5	0.0	7.91806472996322E-5	7.198240663602927E-6
16-17	0.0021792673609057865	2.6993402488510975E-5	0.0	8.098020746553293E-5	7.198240663602927E-6
18-19	0.003719690862916813	3.7790763483915374E-5	0.0	8.997800829503658E-5	7.198240663602927E-6
20-21	0.005643420680264695	4.498900414751829E-5	0.0	1.0797360995404392E-4	7.198240663602927E-6
22-23	0.008099820306719194	4.858812447931976E-5	0.0	1.1697141078354756E-4	7.198240663602927E-6
24-25	0.011060096779625897	5.2187244811121225E-5	0.0	1.3136789211075342E-4	7.198240663602927E-6
26-27	0.014218324870781682	5.398680497702196E-5	0.0	1.3676657260845563E-4	8.997800829503658E-6
28-29	0.01758530194118195	5.758592530882342E-5	0.0	1.421652531061578E-4	1.2596921161305123E-5
30-31	0.020464598206623122	6.118504564062488E-5	0.0	1.457643734379593E-4	1.4396481327205854E-5
32-33	0.022823821584118983	6.478416597242635E-5	0.0	1.4756393360386002E-4	1.4396481327205854E-5
34-35	0.025037280588176884	6.838328630422782E-5	0.0	1.5116305393566147E-4	1.799560165900732E-5
36-37	0.027274133874391492	6.838328630422782E-5	0.0	1.583612945992644E-4	1.799560165900732E-5
38-39	0.0295037889199425	7.198240663602928E-5	0.0	1.7275777592647026E-4	1.799560165900732E-5
40-41	0.03154449014807393	7.558152696783073E-5	0.0	1.7995601659007317E-4	2.1594721990808783E-5
42-43	0.03373095574964332	8.277976763143367E-5	0.0	1.8355513692187465E-4	2.1594721990808783E-5
44-45	0.03602899408149855	8.277976763143367E-5	0.0	1.9075337758547758E-4	2.1594721990808783E-5
46-47	0.03853578139259828	8.277976763143367E-5	0.0	1.979516182490805E-4	2.1594721990808783E-5
48-49	0.041404280297044044	8.45793277973344E-5	0.0	2.1774678007398856E-4	2.1594721990808783E-5
50-51	0.044701074520974174	8.637888796323513E-5	0.0	2.2134590040579004E-4	2.3394282156709516E-5
52-53	0.04850354515152243	8.817844812913586E-5	0.0	2.2674458090349222E-4	2.6993402488510975E-5
54-55	0.05325978266999806	9.717624895863952E-5	0.0	2.3574238173299587E-4	2.879296265441171E-5
56-57	0.05885101610545163	1.0977317011994466E-4	0.0	2.3934150206479735E-4	2.879296265441171E-5
58-59	0.06538521906783719	1.187709709494483E-4	0.0	2.429406223965988E-4	2.879296265441171E-5
60-61	0.07379816284342311	1.3136789211075342E-4	3.5991203318014636E-6	2.5193842322610244E-4	2.879296265441171E-5
62-63	0.08369034507537944	1.3316745227665415E-4	3.5991203318014636E-6	2.609362240556061E-4	3.2392082986213175E-5
64-65	0.09573660082591894	1.3676657260845563E-4	3.5991203318014636E-6	2.645353443874076E-4	3.599120331801464E-5
66-67	0.11087270138130999	1.3856613277435636E-4	5.398680497702196E-6	2.75332705382812E-4	3.95903236498161E-5
68-69	0.12927320407764498	1.4756393360386002E-4	7.198240663602927E-6	2.771322655487127E-4	4.6788564313419026E-5
70-71	0.15149957168668493	1.5476217426746295E-4	7.198240663602927E-6	2.8972918671001785E-4	4.6788564313419026E-5
72-73	0.17863873854863385	1.6016085476516513E-4	1.2596921161305123E-5	2.9692742737362076E-4	4.6788564313419026E-5
74-75	0.2116444715514192	1.6196041493106588E-4	1.4396481327205854E-5	3.095243485349259E-4	4.6788564313419026E-5
76-77	0.2506715328693083	1.6555953526286733E-4	1.4396481327205854E-5	3.167225891985288E-4	5.038768464522049E-5
78-79	0.29719376227817407	1.74557336092371E-4	1.4396481327205854E-5	3.167225891985288E-4	5.2187244811121225E-5
80-81	0.3528253652468293	1.8355513692187465E-4	1.4396481327205854E-5	3.185221493644295E-4	6.118504564062488E-5
82-83	0.4180612208208967	1.8355513692187465E-4	1.4396481327205854E-5	3.2931951035983394E-4	6.478416597242635E-5
84-85	0.49343039968915114	1.9075337758547758E-4	1.6196041493106587E-5	3.3651775102343684E-4	6.478416597242635E-5
86-87	0.5803293605403317	1.9615205808317976E-4	1.799560165900732E-5	3.49114672184742E-4	6.478416597242635E-5
88	0.6535678601721596	2.1594721990808783E-4	1.799560165900732E-5	3.5271379251654343E-4	6.478416597242635E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	16870	0.0	36.94879	1
TCTCGAG	18530	0.0	33.38218	2
GATCCGG	25415	0.0	25.415071	1
CTCGAGG	25070	0.0	24.827394	3
TATCTCG	8750	0.0	24.227167	2
ATCCGGC	28910	0.0	22.274431	2
TCGAGGG	28650	0.0	21.675827	4
TCCGGCG	33470	0.0	19.32681	3
GCGCCGT	36140	0.0	17.63629	9
ATATCTC	14115	0.0	15.976511	1
CGAGGGC	39650	0.0	15.662356	5
CGGCGCG	41920	0.0	15.2852335	5
CGTCGAC	33600	0.0	15.128709	12-13
GGATCCG	17650	0.0	14.96669	2
GCCGTCG	35440	0.0	14.555366	10-11
TATGGAT	16070	0.0	14.478497	2
AGGGCGC	44125	0.0	14.44472	2
CCGGCGC	45310	0.0	14.255753	4
TCGACTT	36710	0.0	13.957673	14-15
GAGGGCG	47010	0.0	13.710096	1
>>END_MODULE