FastQCFastQC Report
Wed 25 May 2016
SRR491025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25602797
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA161700.041.4302061
TCTCGAG182500.036.470282
CTCGAGG233650.028.5908623
GATCCGG261900.026.8018251
TCGAGGG278700.023.8512654
ATCCGGC312050.022.4741552
TATCTCG99400.020.4757692
TCCGGCG358000.019.5397433
GCGCCGT411250.016.835749
GGATCCG177350.016.2203032
CGAGGGC417900.016.1652875
ATATCTC137100.015.7614951
CGGCGCG448700.015.5061745
AGGGCGC434350.015.2038077
TATGGAT158550.014.9418032
CGTCGAC391750.014.73343112-13
GCCGTCG407250.014.276463510-11
TCGACTT411200.014.12394314-15
ATATGGA181850.013.6433031
GAGGGCG500150.013.4480546
Produced by FastQC (version 0.11.5)