##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491025_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25602797
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.662938232881352	33.0	31.0	34.0	29.0	34.0
2	31.914791692485785	34.0	31.0	34.0	30.0	34.0
3	31.981913343296046	34.0	31.0	34.0	30.0	34.0
4	35.352767160556716	37.0	35.0	37.0	33.0	37.0
5	35.133346055901626	37.0	35.0	37.0	32.0	37.0
6	35.06918830001269	37.0	35.0	37.0	32.0	37.0
7	34.9909675493658	37.0	35.0	37.0	32.0	37.0
8	34.9060029652229	37.0	35.0	37.0	32.0	37.0
9	36.42983151411153	39.0	37.0	39.0	32.0	39.0
10-11	36.25926208374811	39.0	37.0	39.0	32.0	39.0
12-13	36.10992830197419	39.0	37.0	39.0	31.5	39.0
14-15	37.39275458849281	40.0	38.0	41.0	32.0	41.0
16-17	37.26931059524473	40.0	38.0	41.0	31.5	41.0
18-19	37.112915456073026	40.0	38.0	41.0	31.0	41.0
20-21	36.956012384115695	40.0	38.0	41.0	30.5	41.0
22-23	36.74805449967049	40.0	37.5	41.0	30.0	41.0
24-25	36.52644402484619	40.0	37.0	41.0	29.5	41.0
26-27	36.15799394886426	40.0	36.5	41.0	27.5	41.0
28-29	35.82907295245906	40.0	36.0	41.0	27.0	41.0
30-31	35.520794427265116	39.5	35.5	41.0	25.5	41.0
32-33	35.287939106809304	39.0	35.0	41.0	25.0	41.0
34-35	35.064613292055554	39.0	35.0	41.0	24.0	41.0
36-37	34.73665914313971	39.0	35.0	40.5	22.5	41.0
38-39	34.37810698963867	39.0	34.5	40.0	19.0	41.0
40-41	33.89337116175237	38.0	34.0	40.0	14.5	41.0
42-43	33.47298051068405	38.0	33.0	40.0	8.5	41.0
44-45	32.98721499061216	38.0	33.0	40.0	2.0	41.0
46-47	32.41880859735754	38.0	32.5	40.0	2.0	41.0
48-49	31.86928549251865	38.0	31.0	40.0	2.0	41.0
50-51	31.380358813921774	37.5	30.5	40.0	2.0	41.0
52-53	30.80391593543471	37.0	29.5	40.0	2.0	41.0
54-55	30.258320604580817	36.0	28.5	39.0	2.0	40.5
56-57	29.724224798564002	36.0	27.5	39.0	2.0	40.0
58-59	29.160723709210366	35.0	26.0	39.0	2.0	40.0
60-61	28.564790303965616	35.0	25.5	38.0	2.0	40.0
62-63	27.91061845703811	34.0	23.5	38.0	2.0	40.0
64-65	27.24631343989487	34.0	21.5	38.0	2.0	40.0
66-67	26.602537527442802	33.5	19.0	37.0	2.0	39.0
68-69	25.85409623409505	32.5	13.0	36.0	2.0	39.0
70-71	25.068236489942876	32.0	7.5	36.0	2.0	38.5
72-73	24.306557365587828	31.0	2.0	35.0	2.0	38.0
74-75	23.490229368299097	30.5	2.0	35.0	2.0	37.0
76-77	22.52320105885306	29.0	2.0	34.0	2.0	36.0
78-79	22.49423535639485	30.0	2.0	34.0	2.0	36.0
80-81	22.042725234278116	29.0	2.0	34.0	2.0	36.0
82-83	21.467062719748938	29.0	2.0	34.0	2.0	35.0
84-85	20.665065969159542	28.5	2.0	34.0	2.0	35.0
86-87	19.93371296503269	26.5	2.0	33.0	2.0	35.0
88-89	19.277595002608503	26.0	2.0	33.0	2.0	35.0
90-91	18.42900373345928	25.0	2.0	32.0	2.0	34.0
92-93	17.609518620172633	22.5	2.0	32.0	2.0	34.0
94-95	16.647675876975473	18.5	2.0	31.5	2.0	34.0
96-97	15.637264905080489	4.5	2.0	31.0	2.0	34.0
98-99	14.678249235815915	2.0	2.0	31.0	2.0	34.0
100	13.833731720795974	2.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1965437772448979
1101	2	0.22106567399805144
1101	3	0.17852845741910528
1101	4	0.18774835872684292
1101	5	0.21520903458472418
1101	6	0.25177253113505316
1101	7	0.2749848076364074
1101	8	0.26636691183291816
1101	9	0.29385097498168733
1101	10-11	0.3212845682188643
1101	12-13	0.3286876385689723
1101	14-15	0.348596038815117
1101	16-17	0.3529979184080574
1101	18-19	0.3701077247921134
1101	20-21	0.35825777894604727
1101	22-23	0.33147857503795564
1101	24-25	0.3433792058849221
1101	26-27	0.33407150250297235
1101	28-29	0.3105368571371798
1101	30-31	0.29096700562908495
1101	32-33	0.27958626180701884
1101	34-35	0.25901362044869813
1101	36-37	0.26226984138784104
1101	38-39	0.19944286395003274
1101	40-41	0.23141154489642446
1101	42-43	0.07122281725324342
1101	44-45	0.005855725570221182
1101	46-47	0.012147971492218801
1101	48-49	0.014685549484905636
1101	50-51	-0.07723574744859008
1101	52-53	-0.21080383325571006
1101	54-55	-0.28892625340998634
1101	56-57	-0.2787313738331001
1101	58-59	-0.3840814513728148
1101	60-61	-0.5072022728066798
1101	62-63	-0.5703337690343346
1101	64-65	-0.5235317379433049
1101	66-67	-0.5663744899870693
1101	68-69	-0.6774859384770551
1101	70-71	-0.6498287793886561
1101	72-73	-0.7961906023705438
1101	74-75	-0.7819700035296897
1101	76-77	-0.6408861451905672
1101	78-79	-0.7116079856228943
1101	80-81	-0.7924883429993699
1101	82-83	-0.7638737875024795
1101	84-85	-0.8226630618952271
1101	86-87	-0.9470389149644376
1101	88-89	-0.8477504552153974
1101	90-91	-0.7673784049605672
1101	92-93	-0.750760821141732
1101	94-95	-0.7767196882620233
1101	96-97	-0.7415949472786334
1101	98-99	-0.7661234317860615
1101	100	-0.8469471771559558
1102	1	0.13954774671336878
1102	2	0.1498092767086341
1102	3	0.14542750900984203
1102	4	0.11767675809141309
1102	5	0.15355670584916936
1102	6	0.12639008886797853
1102	7	0.15451526729220433
1102	8	0.1269128944573623
1102	9	0.12217375862649504
1102	10-11	0.10376122304678148
1102	12-13	0.1494886907255193
1102	14-15	0.14770708650638653
1102	16-17	0.1365688204269091
1102	18-19	0.18349944820314334
1102	20-21	0.13161107713609255
1102	22-23	0.15051526223910372
1102	24-25	0.16863240821572845
1102	26-27	0.1621195784899001
1102	28-29	0.09745340882747655
1102	30-31	0.05890131006724886
1102	32-33	0.06115006695026892
1102	34-35	5.922626974594891E-4
1102	36-37	0.0698181276052452
1102	38-39	0.027518977503866893
1102	40-41	0.07445681175642704
1102	42-43	-0.013390324027426459
1102	44-45	-0.08332346694044901
1102	46-47	0.002560600774437205
1102	48-49	1.2246757071210368E-4
1102	50-51	-0.05965600386630143
1102	52-53	-0.07709366562304965
1102	54-55	-0.10834115895652374
1102	56-57	-0.09845534339804374
1102	58-59	-0.22547845073637163
1102	60-61	-0.23836280449137348
1102	62-63	-0.2568364727383141
1102	64-65	-0.2527595425597333
1102	66-67	-0.2801013529340679
1102	68-69	-0.2960821341203719
1102	70-71	-0.366097264709456
1102	72-73	-0.370890573693778
1102	74-75	-0.27275243368309177
1102	76-77	-0.24917293528951134
1102	78-79	-0.24544885009050077
1102	80-81	-0.32193954874229647
1102	82-83	-0.23966068656362438
1102	84-85	-0.28465198959117544
1102	86-87	-0.3543973942998342
1102	88-89	-0.24729460956239535
1102	90-91	-0.08624652377899267
1102	92-93	-0.09547906317992272
1102	94-95	-0.1448491928274933
1102	96-97	-0.13528350613656137
1102	98-99	-0.013337039750268787
1102	100	-0.04831004239322212
1103	1	0.045547649606128005
1103	2	0.07294963703788326
1103	3	0.0644051953189333
1103	4	0.05211027533415091
1103	5	0.11541892814801002
1103	6	0.09015504248086614
1103	7	0.07493881226027099
1103	8	0.032974004137741986
1103	9	0.08199664955419195
1103	10-11	0.0611375349264307
1103	12-13	0.09724469885577491
1103	14-15	0.10763443997384314
1103	16-17	0.14393813980517223
1103	18-19	0.1689089659438494
1103	20-21	0.1451653376265014
1103	22-23	0.1321953699533509
1103	24-25	0.11232260468827349
1103	26-27	0.1970005112669071
1103	28-29	0.1642869208119606
1103	30-31	0.1891060989129727
1103	32-33	0.07269142369300141
1103	34-35	0.04156309946500869
1103	36-37	0.061419589916852146
1103	38-39	0.1459000310204388
1103	40-41	0.18616794150126736
1103	42-43	0.11350715089164964
1103	44-45	-0.014535935264703426
1103	46-47	0.09065617697120842
1103	48-49	-0.03716198551918737
1103	50-51	0.08159016175239842
1103	52-53	0.018470346854382313
1103	54-55	-0.04877372387641188
1103	56-57	0.05226269086021418
1103	58-59	0.02992629072984343
1103	60-61	-0.09677981594897034
1103	62-63	0.04898763302089293
1103	64-65	0.06950452488301906
1103	66-67	0.033447401678508015
1103	68-69	-0.004295827619614556
1103	70-71	0.0029949325630198587
1103	72-73	-0.049598427525960886
1103	74-75	-0.07147555169978759
1103	76-77	-0.07077506581073933
1103	78-79	-0.08468492644350789
1103	80-81	-0.06671704060343941
1103	82-83	0.003531099399797455
1103	84-85	-0.1673172578873512
1103	86-87	-0.1061603913675242
1103	88-89	0.09808475752411638
1103	90-91	0.2567970175783181
1103	92-93	0.35652339292835933
1103	94-95	0.20200303342005554
1103	96-97	0.2470216546179831
1103	98-99	0.21193813013786666
1103	100	0.2555163647409131
1104	1	-0.00707665803113855
1104	2	0.018367423358235158
1104	3	-0.018601551776647796
1104	4	-0.010268146503051412
1104	5	-0.011447101356708345
1104	6	-0.010979864973570841
1104	7	-0.030479910076692818
1104	8	0.0035073456421912397
1104	9	-0.04653874854043494
1104	10-11	-0.023145272523805716
1104	12-13	0.012939647416644107
1104	14-15	0.06956616582733943
1104	16-17	0.1019169056411755
1104	18-19	0.09869920813742539
1104	20-21	0.09767358029138506
1104	22-23	0.10390136771543013
1104	24-25	0.12590752275627892
1104	26-27	0.19144737882948704
1104	28-29	0.045837495951928986
1104	30-31	0.09348763635868096
1104	32-33	0.08763640072498191
1104	34-35	0.07833773394408183
1104	36-37	0.10802556540413377
1104	38-39	0.1401989376301387
1104	40-41	0.20791203789459445
1104	42-43	0.13366960215748236
1104	44-45	0.10061307490044413
1104	46-47	0.16619857403760818
1104	48-49	0.17609303472633897
1104	50-51	0.13104989945277623
1104	52-53	0.06952192726060957
1104	54-55	0.10983374459723905
1104	56-57	0.1262463420358948
1104	58-59	0.14147475004450527
1104	60-61	0.10308266537488109
1104	62-63	0.10880515944619162
1104	64-65	0.1649113459971403
1104	66-67	0.15370563967146467
1104	68-69	0.16827470220185603
1104	70-71	0.19311831329189033
1104	72-73	0.15897607735723085
1104	74-75	0.33051837303762355
1104	76-77	0.30592662879441335
1104	78-79	0.24114373238044706
1104	80-81	0.22959389026707555
1104	82-83	0.3352365740511196
1104	84-85	0.3043399116727272
1104	86-87	0.28832689039799675
1104	88-89	0.39896365065945716
1104	90-91	0.48931634997371276
1104	92-93	0.4680474893474269
1104	94-95	0.43229632835278053
1104	96-97	0.5327282495528589
1104	98-99	0.5312518432651991
1104	100	0.6445776620653767
1105	1	-0.0635893462739503
1105	2	-0.05068421250614463
1105	3	-0.1171942477166894
1105	4	-0.07154275967166512
1105	5	-0.0707357687294774
1105	6	-0.0989466884363992
1105	7	-0.11671381275284176
1105	8	-0.050215236978921496
1105	9	-0.07806330617850676
1105	10-11	-0.09679825306149326
1105	12-13	-0.08512897870045322
1105	14-15	-0.07082988407152868
1105	16-17	-0.08785033642084272
1105	18-19	-0.0312682562349309
1105	20-21	-0.024053953709611164
1105	22-23	-0.05305791056753861
1105	24-25	-0.0789977624520759
1105	26-27	-0.1895751489676556
1105	28-29	-0.1633641793185845
1105	30-31	-0.059141063970976404
1105	32-33	-0.07277252079895646
1105	34-35	-0.0757799107128676
1105	36-37	-0.06061285587413323
1105	38-39	-0.0101159174555292
1105	40-41	0.06900480881913751
1105	42-43	0.016074794569888695
1105	44-45	-0.03826392596957362
1105	46-47	0.06766590098460057
1105	48-49	0.04335164374092315
1105	50-51	0.043142520845201204
1105	52-53	0.0077514048346500886
1105	54-55	0.01851883589240444
1105	56-57	0.06587367268229016
1105	58-59	0.07851863991338703
1105	60-61	0.06156747874094037
1105	62-63	0.07128996615816163
1105	64-65	0.118931717483715
1105	66-67	0.041127201053253515
1105	68-69	0.07165478167080863
1105	70-71	0.1364908756592058
1105	72-73	0.16836402420835128
1105	74-75	0.34847548724690824
1105	76-77	0.33071219118715334
1105	78-79	0.25621754762660487
1105	80-81	0.16626164528020126
1105	82-83	0.17917764915560852
1105	84-85	0.17836818858402737
1105	86-87	0.24719504721378627
1105	88-89	0.40225564749527365
1105	90-91	0.36375113697463135
1105	92-93	0.3040415928519806
1105	94-95	0.44075743821513313
1105	96-97	0.5624109828648454
1105	98-99	0.4851513405775094
1105	100	0.5962044274840679
1106	1	-0.22765305620455223
1106	2	-0.2117466141312505
1106	3	-0.226109285128544
1106	4	-0.23804071821408712
1106	5	-0.2652664548851362
1106	6	-0.28380061502365095
1106	7	-0.26799146329497603
1106	8	-0.2852753438667577
1106	9	-0.3293912961517549
1106	10-11	-0.3372144540430497
1106	12-13	-0.34388742535528394
1106	14-15	-0.41255612984597434
1106	16-17	-0.5013934678781311
1106	18-19	-0.6471451819799938
1106	20-21	-0.5951239042194842
1106	22-23	-0.6125728763293736
1106	24-25	-0.7351004955809657
1106	26-27	-0.843788362725526
1106	28-29	-0.654397305055042
1106	30-31	-0.7616884557749515
1106	32-33	-0.7437721274319173
1106	34-35	-0.5890568330135721
1106	36-37	-0.6311478298197954
1106	38-39	-0.544193408928173
1106	40-41	-0.6523825998827633
1106	42-43	-0.7497221778566256
1106	44-45	-0.7040406141159323
1106	46-47	-0.6186760399760303
1106	48-49	-0.6232203829296914
1106	50-51	-0.66418419192469
1106	52-53	-0.7507810189310717
1106	54-55	-0.7102571881910649
1106	56-57	-0.5257678988354222
1106	58-59	-0.5907236084312899
1106	60-61	-0.5386522428094551
1106	62-63	-0.5805960763673959
1106	64-65	-0.5536888017212966
1106	66-67	-0.6264868720833192
1106	68-69	-0.6054262720224735
1106	70-71	-0.6296929441387569
1106	72-73	-0.6185395821786628
1106	74-75	-0.3515338092897018
1106	76-77	-0.43098009144475924
1106	78-79	-0.5094577010674861
1106	80-81	-0.5464891779548893
1106	82-83	-0.45139509653374077
1106	84-85	-0.4652085963215171
1106	86-87	-0.37784556374619527
1106	88-89	-0.22629793360950856
1106	90-91	-0.33168641225337225
1106	92-93	-0.32424004591684863
1106	94-95	-0.32753973604065223
1106	96-97	-0.31199176085715585
1106	98-99	-0.3821840647091861
1106	100	-0.2983203960634828
1107	1	-0.30354197000121275
1107	2	-0.23330513857537483
1107	3	-0.27059171006298754
1107	4	-0.2360392138360936
1107	5	-0.24732960740495713
1107	6	-0.24678036416172233
1107	7	-0.26822550102283316
1107	8	-0.2763847937319923
1107	9	-0.3035397819826997
1107	10-11	-0.3012402074711531
1107	12-13	-0.3121344731969913
1107	14-15	-0.3203900291412225
1107	16-17	-0.3133982051194195
1107	18-19	-0.3021668048825674
1107	20-21	-0.2833060715359821
1107	22-23	-0.38627034669837457
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2207	94-95	1.0002944954418957
2207	96-97	0.9377918622087709
2207	98-99	1.0004189657240552
2207	100	0.9761624291452708
2208	1	-0.8070925480824549
2208	2	-0.6537947002608107
2208	3	-0.683319784702654
2208	4	-0.635917636887946
2208	5	-0.7123651698631548
2208	6	-0.7026742954653002
2208	7	-0.7420200639862031
2208	8	-0.7102752046438923
2208	9	-0.7966397647344508
2208	10-11	-0.9284873906742703
2208	12-13	-0.9675782631932606
2208	14-15	-1.0984295184393602
2208	16-17	-0.9901982091822816
2208	18-19	-1.0137040532229875
2208	20-21	-1.1240262216109116
2208	22-23	-0.9567888972126539
2208	24-25	-1.035322604925696
2208	26-27	-1.3454285424285928
2208	28-29	-1.2549490584128122
2208	30-31	-1.2749683110253756
2208	32-33	-1.306777660314829
2208	34-35	-0.9870957314582469
2208	36-37	-1.3729815286693565
2208	38-39	-1.3801224855701903
2208	40-41	-2.0196919098879818
2208	42-43	-1.1666247720326481
2208	44-45	-0.8920032135441716
2208	46-47	-1.4545885243352537
2208	48-49	-1.5352608506604248
2208	50-51	-1.5051406789882265
2208	52-53	-0.8252599387106443
2208	54-55	-0.6312041052078889
2208	56-57	-0.532213060802011
2208	58-59	-0.6962768071145327
2208	60-61	-0.5774376046652065
2208	62-63	-0.7382969273741367
2208	64-65	-0.7982831401296515
2208	66-67	-0.700027074975921
2208	68-69	-1.0942090059257907
2208	70-71	-1.0744565996658437
2208	72-73	-0.8343594282257527
2208	74-75	-1.7113194782276189
2208	76-77	-1.190439783689417
2208	78-79	-1.586291776108954
2208	80-81	-1.5618949521242413
2208	82-83	-1.5341662477346105
2208	84-85	-2.1134674886843356
2208	86-87	-1.9238138986430613
2208	88-89	-2.073043424709777
2208	90-91	-2.1339295966094376
2208	92-93	-2.770808408844516
2208	94-95	-2.8453409217721717
2208	96-97	-2.797562612496341
2208	98-99	-2.823120227814236
2208	100	-2.8815292711653786
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	369965.0
3	164866.0
4	162244.0
5	133744.0
6	122495.0
7	124821.0
8	130440.0
9	145053.0
10	167078.0
11	189345.0
12	211209.0
13	244657.0
14	297803.0
15	374796.0
16	443987.0
17	449736.0
18	420229.0
19	401167.0
20	405778.0
21	422409.0
22	441359.0
23	463742.0
24	488473.0
25	518792.0
26	554435.0
27	602909.0
28	667052.0
29	754935.0
30	866128.0
31	1007146.0
32	1188385.0
33	1449918.0
34	1860798.0
35	2516707.0
36	3191245.0
37	2753319.0
38	865717.0
39	29905.0
40	10.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.85145804317906	27.849208174431837	27.686087409243314	22.61324637314578
2	23.748409207009686	27.722037557068475	26.987731067039277	21.54182216888256
3	23.429765425222953	26.22348345967516	26.890675624587708	23.456075490514177
4	24.268475830773937	25.880529671652763	26.516280492673193	23.33471400490011
5	24.5383555911283	26.167424757860342	26.27106718967354	23.02315246133782
6	24.525553922901448	25.97473701363923	25.74546409875898	23.754244964700337
7	24.47357365445865	26.330933475902008	25.88785793585597	23.30763493378337
8	23.930039908137697	26.138998217156328	26.215235056165593	23.715726818540386
9	23.91207756643892	25.842116982503015	26.365702164563043	23.880103286495018
10-11	23.76953083729253	26.116090391101267	26.29344896405853	23.820929807547678
12-13	23.531862678373297	26.14359729367574	26.473416062378114	23.851123965572846
14-15	23.285892386180514	26.32843662332632	26.914882463569402	23.470788526923766
16-17	23.13980383959571	26.273393092856978	27.042459381042278	23.544343686505034
18-19	22.790297006770597	26.221874210896033	27.65863955693588	23.329189225397485
20-21	22.53219689689774	25.95617045405894	28.404930344420038	23.106702304623276
22-23	22.540980949109475	25.933009978829567	28.720891825325133	22.805117246735822
24-25	22.503836201855894	25.779612380930878	29.07937092460921	22.637180492604017
26-27	22.53155253635497	25.819529703880534	29.116441491139796	22.532476268624706
28-29	22.58775489606835	25.87407668258629	29.083102199651066	22.455066221694295
30-31	22.551907399373157	25.796669251405223	29.03449420025484	22.616929148966776
32-33	22.562893359665914	25.873837306464637	28.981201309353455	22.582068024515994
34-35	22.634791693137267	25.918211413888148	28.95992434430015	22.487072548674433
36-37	22.667578579490392	25.858915425892302	28.862966558446512	22.610539436170793
38-39	22.71255484519336	25.934724967967306	28.735028216700982	22.61769197013836
40-41	22.797122175259855	26.057545792736438	28.494871132277073	22.65046089972664
42-43	22.946649850393012	26.138894475890815	28.058474418232958	22.85598125548321
44-45	23.03732464841802	26.34315489932978	27.642988206012287	22.97653224623991
46-47	23.238466229419398	26.52018379336944	27.223218155670825	23.01813182154034
48-49	23.37024420223365	26.516652680498083	26.889520829743535	23.22358228752473
50-51	23.435612289364194	26.560553028536617	26.68958661462956	23.314248067469627
52-53	23.51949969151768	26.62970099634555	26.554955160310033	23.295844151826735
54-55	23.569581231396914	26.52079283091616	26.514320451638483	23.39530548604844
56-57	23.575209978296282	26.590332274622735	26.48497743992224	23.349480307158743
58-59	23.62000623373344	26.645775059718783	26.46950676458121	23.264711941966553
60-61	23.59694330168781	26.591848094926373	26.37027338217562	23.440935221210193
62-63	23.613338494502802	26.684052706968746	26.32985212179274	23.372756676735712
64-65	23.671642035306746	26.757851173350506	26.231573692137054	23.33893309920569
66-67	23.72394111686658	26.748258535774085	26.12174465846944	23.4060556888899
68-69	23.735372662841066	26.829381038770283	26.030909246966093	23.404337051422548
70-71	23.782168956988976	26.894328653110332	25.917402622908636	23.406099766992057
72-73	23.77138216312388	26.913220261699998	25.77880638149454	23.536591193681584
74-75	23.85651688176612	26.9379378966493	25.741333193967293	23.464212027617286
76-77	23.35916452247616	27.328467851789647	25.54294809512882	23.769419530605372
78-79	23.55301434897396	27.270618807131374	25.399968066733692	23.776398777160974
80-81	23.52829430510616	27.346837339176705	25.389677330524922	23.73519102519221
82-83	23.578904593424962	27.486360502912216	25.228834228936698	23.705900674726124
84-85	23.514852451944872	27.494594879405486	25.070539782445312	23.920012886204333
86-87	23.42885098879377	27.612703343276777	25.042223486597397	23.91622218133205
88-89	23.40885031899969	27.70875543493704	24.912768082630084	23.969626163433183
90-91	23.393151727847684	27.684949546538313	24.762533920377784	24.159364805236216
92-93	23.345449091406024	27.77141496775733	24.65878450723191	24.22435143360473
94-95	23.358729136483323	27.84880579807692	24.467226375825387	24.325238689614366
96-97	23.268601222938834	27.93036661409088	24.285091449337624	24.515940713632663
98-99	23.211693571148505	27.96751132979802	24.182926542953616	24.63786855609986
100	23.970383754274643	28.006405611351166	23.486029413516725	24.537181220857462
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1036.0
1	751.0
2	391.0
3	329.5
4	372.0
5	454.5
6	569.0
7	682.5
8	858.0
9	1135.5
10	1483.0
11	1931.5
12	2623.5
13	3669.5
14	5802.0
15	8586.5
16	12680.0
17	16062.5
18	18520.0
19	23346.5
20	33465.5
21	42082.0
22	49423.5
23	62093.0
24	76488.0
25	93381.5
26	114293.0
27	142751.5
28	176498.5
29	208858.0
30	240893.0
31	278872.5
32	326455.0
33	377934.5
34	428208.0
35	481813.0
36	543381.0
37	619894.0
38	698673.0
39	755749.0
40	800620.5
41	853203.5
42	931696.0
43	1006458.0
44	1037376.5
45	1047130.5
46	1050846.0
47	1038904.5
48	1013672.5
49	986558.0
50	943297.5
51	888892.5
52	829629.5
53	758667.5
54	698582.0
55	644015.0
56	585556.0
57	531761.5
58	486360.5
59	444857.0
60	400621.0
61	359277.5
62	323816.5
63	291893.0
64	260619.0
65	230207.5
66	202293.0
67	175466.5
68	150025.0
69	127491.0
70	108227.5
71	91890.5
72	78512.0
73	67591.0
74	57241.5
75	47321.5
76	39176.0
77	32765.5
78	27482.5
79	23134.0
80	19100.0
81	15365.5
82	12181.5
83	9252.0
84	6780.0
85	4813.0
86	3322.0
87	2244.5
88	1535.5
89	1038.0
90	705.5
91	500.0
92	335.5
93	214.5
94	139.5
95	79.0
96	41.5
97	26.0
98	11.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15438938175387634
2	0.0
3	0.0016716923545501688
4	0.0067531684135916864
5	0.020204823715158932
6	0.0038823883187450187
7	6.444608376186399E-4
8	0.003909729081553082
9	0.01600215788923374
10-11	5.624385491944493E-4
12-13	3.9058232582947874E-4
14-15	0.009682535857312777
16-17	4.979924654325854E-4
18-19	7.108598330096513E-4
20-21	0.01220569768217121
22-23	0.007807740693331279
24-25	0.0025426909411499065
26-27	5.487681677904176E-4
28-29	3.515240932465308E-5
30-31	0.002322011927056251
32-33	0.005102958086962139
34-35	0.0019372883361142145
36-37	7.948350330629892E-4
38-39	2.0310280943132894E-4
40-41	0.0010350431634481186
42-43	8.456107354208214E-4
44-45	3.5542991650482563E-4
46-47	0.0013670381404031755
48-49	0.00280242818782651
50-51	0.0036538976581347734
52-53	1.28892167523728E-4
54-55	0.00664575827398858
56-57	0.0010545722797395925
58-59	0.006899636785777741
60-61	0.0023337293968311353
62-63	2.8317218622637204E-4
64-65	9.315388471033066E-4
66-67	0.0019177592198227405
68-69	0.0010330902518189712
70-71	0.005952474645641255
72-73	9.237272005867171E-4
74-75	0.0044956025702973
76-77	0.002624713229574097
78-79	0.009899309048148137
80-81	0.0044311564865354355
82-83	1.5037419544434928E-4
84-85	8.709985865997375E-4
86-87	0.0013279799078202276
88-89	0.001950958717518246
90-91	7.53823888850894E-4
92-93	0.0018142549034779287
94-95	0.0036285098069558574
96-97	0.004007374663010451
98-99	0.016209166521923366
100	0.008272533661068358
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.5602797E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.29633106706727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.13657827819738	32.53138168379449
2	12.773287146427009	11.060729382128375
3	4.752283579269747	6.172693295179513
4	2.2661945837062114	3.924716438341554
5	1.2033555045448314	2.60504391580754
6	0.7398940256343055	1.922081801304483
7	0.48985136800745765	1.4846136902034686
8	0.3605430791231991	1.248815401412629
9	0.2695213987489005	1.0502358938902303
>10	1.6960130109895615	14.112028943244109
>50	0.12385418638671221	3.735352318551911
>100	0.17358683815387946	15.385441572792935
>500	0.014364177664191876	3.8955095548347045
>1k	5.462160802628361E-4	0.3449716604542052
>5k	8.139025709899807E-5	0.2639439892635103
>10k+	4.521680949777904E-5	0.26244045879636124
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.905823258294787E-6	0.0
2	3.905823258294787E-6	0.0	0.0	7.811646516589574E-6	0.0
3	3.905823258294787E-6	0.0	0.0	1.171746977488436E-5	0.0
4	3.905823258294787E-6	0.0	0.0	1.9529116291473937E-5	0.0
5	7.811646516589574E-6	3.905823258294787E-6	0.0	1.9529116291473937E-5	0.0
6	7.811646516589574E-6	7.811646516589574E-6	0.0	2.734076280806351E-5	0.0
7	1.562329303317915E-5	7.811646516589574E-6	0.0	3.515240932465308E-5	0.0
8	1.9529116291473937E-5	7.811646516589574E-6	0.0	3.515240932465308E-5	0.0
9	3.12465860663583E-5	7.811646516589574E-6	0.0	3.515240932465308E-5	0.0
10-11	1.3865672566946494E-4	9.764558145736968E-6	0.0	4.296405584124266E-5	0.0
12-13	4.6284005610793227E-4	1.171746977488436E-5	0.0	4.686987909953744E-5	0.0
14-15	0.00114635912630952	1.171746977488436E-5	0.0	5.8587348874421804E-5	0.0
16-17	0.002361070159639199	1.171746977488436E-5	0.0	5.8587348874421804E-5	0.0
18-19	0.004237818235249844	1.171746977488436E-5	0.0	5.8587348874421804E-5	0.0
20-21	0.006550065604160358	1.171746977488436E-5	0.0	5.8587348874421804E-5	0.0
22-23	0.009288047708225004	1.3670381404031755E-5	0.0	6.639899539101138E-5	0.0
24-25	0.012195933124025473	1.9529116291473937E-5	0.0	6.639899539101138E-5	0.0
26-27	0.0155842348005962	1.9529116291473937E-5	1.9529116291473936E-6	7.030481864930616E-5	0.0
28-29	0.019267426133168182	2.148202792062133E-5	3.905823258294787E-6	7.421064190760095E-5	0.0
30-31	0.022446766265420144	2.5387851178916114E-5	3.905823258294787E-6	7.421064190760095E-5	0.0
32-33	0.025145690136901842	2.734076280806351E-5	3.905823258294787E-6	7.421064190760095E-5	0.0
34-35	0.027653228668727093	2.734076280806351E-5	3.905823258294787E-6	8.397520005333793E-5	0.0
36-37	0.03014123808426087	2.734076280806351E-5	3.905823258294787E-6	9.17868465699275E-5	0.0
38-39	0.032594095090469996	2.734076280806351E-5	3.905823258294787E-6	1.0350431634481187E-4	3.905823258294787E-6
40-41	0.03502156424550021	2.734076280806351E-5	3.905823258294787E-6	1.0741013960310664E-4	3.905823258294787E-6
42-43	0.03762674835878283	2.734076280806351E-5	3.905823258294787E-6	1.1717469774884361E-4	3.905823258294787E-6
44-45	0.04015186309527041	2.734076280806351E-5	3.905823258294787E-6	1.19127609377991E-4	3.905823258294787E-6
46-47	0.04272189479922838	2.734076280806351E-5	7.811646516589574E-6	1.3475090241117014E-4	3.905823258294787E-6
48-49	0.04580749517328127	3.12465860663583E-5	7.811646516589574E-6	1.4060963729861236E-4	3.905823258294787E-6
50-51	0.049297348254567656	3.12465860663583E-5	7.811646516589574E-6	1.523271070734967E-4	3.905823258294787E-6
52-53	0.053263711773366015	3.515240932465308E-5	7.811646516589574E-6	1.5818584196093888E-4	3.905823258294787E-6
54-55	0.05778470219484223	3.9058232582947874E-5	7.811646516589574E-6	1.7185622336497063E-4	3.905823258294787E-6
56-57	0.0633055833704419	3.9058232582947874E-5	9.764558145736968E-6	1.796678698815602E-4	3.905823258294787E-6
58-59	0.06995524746768877	3.9058232582947874E-5	1.3670381404031755E-5	1.83573693139855E-4	3.905823258294787E-6
60-61	0.07816724086825358	3.9058232582947874E-5	1.562329303317915E-5	1.8552660476900239E-4	3.905823258294787E-6
62-63	0.08797476306983179	3.9058232582947874E-5	1.562329303317915E-5	1.9529116291473937E-4	3.905823258294787E-6
64-65	0.10008281517054562	3.9058232582947874E-5	1.562329303317915E-5	2.0505572106047632E-4	3.905823258294787E-6
66-67	0.11449920881691168	3.9058232582947874E-5	1.7576204662326544E-5	2.2263192572280287E-4	3.905823258294787E-6
68-69	0.13190746307913154	3.9058232582947874E-5	1.9529116291473937E-5	2.3239648386853983E-4	7.811646516589574E-6
70-71	0.15321763477638792	3.9058232582947874E-5	1.9529116291473937E-5	2.499726885308664E-4	1.562329303317915E-5
72-73	0.17879101256007301	3.9058232582947874E-5	2.148202792062133E-5	2.6169015830575075E-4	1.562329303317915E-5
74-75	0.20943219602139562	3.9058232582947874E-5	2.5387851178916114E-5	2.812192745972247E-4	1.7576204662326544E-5
76-77	0.24627973264014866	3.9058232582947874E-5	3.12465860663583E-5	2.968425676304038E-4	2.5387851178916114E-5
78-79	0.2897495925933405	3.9058232582947874E-5	3.319949769550569E-5	3.046542141469934E-4	2.734076280806351E-5
80-81	0.3414900332959715	3.9058232582947874E-5	3.515240932465308E-5	3.1832459555102515E-4	2.734076280806351E-5
82-83	0.4022353495206012	3.9058232582947874E-5	3.515240932465308E-5	3.300420653259095E-4	3.12465860663583E-5
84-85	0.47221207901621065	3.9058232582947874E-5	3.515240932465308E-5	3.456653583590887E-4	3.12465860663583E-5
86-87	0.5529884098210052	4.296405584124266E-5	3.515240932465308E-5	3.5347700487567825E-4	3.12465860663583E-5
88	0.620705620561691	4.296405584124266E-5	3.515240932465308E-5	3.7105320953800477E-4	3.12465860663583E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	16170	0.0	41.430206	1
TCTCGAG	18250	0.0	36.47028	2
CTCGAGG	23365	0.0	28.590862	3
GATCCGG	26190	0.0	26.801825	1
TCGAGGG	27870	0.0	23.851265	4
ATCCGGC	31205	0.0	22.474155	2
TATCTCG	9940	0.0	20.475769	2
TCCGGCG	35800	0.0	19.539743	3
GCGCCGT	41125	0.0	16.83574	9
GGATCCG	17735	0.0	16.220303	2
CGAGGGC	41790	0.0	16.165287	5
ATATCTC	13710	0.0	15.761495	1
CGGCGCG	44870	0.0	15.506174	5
AGGGCGC	43435	0.0	15.203807	7
TATGGAT	15855	0.0	14.941803	2
CGTCGAC	39175	0.0	14.733431	12-13
GCCGTCG	40725	0.0	14.2764635	10-11
TCGACTT	41120	0.0	14.123943	14-15
ATATGGA	18185	0.0	13.643303	1
GAGGGCG	50015	0.0	13.448054	6
>>END_MODULE