FastQCFastQC Report
Wed 25 May 2016
SRR491024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25389531
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA327440.12896654136699098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA161450.039.83831
TCTCGAG182350.034.7427982
GATCCGG243800.027.984841
CTCGAGG232600.027.219043
TCGAGGG261250.024.0718084
ATCCGGC286700.023.8515152
TCCGGCG325600.020.931413
TATCTCG98200.020.1967542
GCGCCGT356650.018.8694489
GGATCCG165600.017.0850722
CGGCGCG395900.017.0594735
CGAGGGC381200.016.496725
CCGGCGC420500.016.2073084
CGTCGAC340850.015.89718312-13
GCCGTCG351850.015.61385910-11
AGGGCGC410900.015.1665047
TCGACTT366050.014.86875214-15
TATGGAT154850.014.2001791
GAGGGCG451250.013.9457656
TGGATCC216600.013.7822131
Produced by FastQC (version 0.11.5)