##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491024_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25389531
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42909500770219	33.0	31.0	34.0	28.0	34.0
2	31.69451164734	34.0	31.0	34.0	28.0	34.0
3	31.767076044059262	34.0	31.0	34.0	28.0	34.0
4	35.139520064391895	37.0	35.0	37.0	32.0	37.0
5	34.89508478908098	37.0	35.0	37.0	32.0	37.0
6	34.78942919426121	37.0	35.0	37.0	32.0	37.0
7	34.74269501079008	37.0	35.0	37.0	32.0	37.0
8	34.63142517283994	37.0	35.0	37.0	32.0	37.0
9	36.11463677686681	39.0	37.0	39.0	31.0	39.0
10-11	35.950970421627716	39.0	37.0	39.0	30.5	39.0
12-13	35.83705468998227	39.0	37.0	39.0	30.0	39.0
14-15	37.06509726390771	40.0	38.0	41.0	31.0	41.0
16-17	36.943854201166616	40.0	38.0	41.0	30.5	41.0
18-19	36.78297381704294	40.0	37.0	41.0	30.0	41.0
20-21	36.662208057328826	40.0	37.0	41.0	30.0	41.0
22-23	36.43537164983473	40.0	37.0	41.0	29.0	41.0
24-25	36.24735488418435	40.0	37.0	41.0	27.5	41.0
26-27	35.90747599867048	40.0	36.0	41.0	27.0	41.0
28-29	35.591237348968754	40.0	36.0	41.0	26.5	41.0
30-31	35.29643064300794	39.0	35.0	41.0	25.0	41.0
32-33	35.074820543160094	39.0	35.0	41.0	24.0	41.0
34-35	34.80641139058457	39.0	35.0	41.0	23.0	41.0
36-37	34.560008946207006	39.0	35.0	40.0	21.0	41.0
38-39	34.228316643580385	39.0	34.0	40.0	17.0	41.0
40-41	33.813609554268645	38.0	34.0	40.0	13.5	41.0
42-43	33.38780367782296	38.0	33.0	40.0	8.5	41.0
44-45	32.93861408861787	38.0	33.0	40.0	2.0	41.0
46-47	32.44077472325109	38.0	32.0	40.0	2.0	41.0
48-49	31.940109153650774	38.0	31.5	40.0	2.0	41.0
50-51	31.44687733696223	37.5	30.5	40.0	2.0	41.0
52-53	30.92754570771709	37.0	30.0	40.0	2.0	41.0
54-55	30.40509326462155	36.5	28.5	39.5	2.0	40.5
56-57	29.916112294472867	36.0	28.0	39.0	2.0	40.0
58-59	29.411481704801872	35.5	27.0	39.0	2.0	40.0
60-61	28.850771465609192	35.0	26.0	38.5	2.0	40.0
62-63	28.233626036652666	34.5	24.5	38.0	2.0	40.0
64-65	27.600816159227204	34.0	22.5	38.0	2.0	40.0
66-67	26.95909672376382	34.0	20.5	37.5	2.0	39.5
68-69	26.26572026872021	33.0	17.5	37.0	2.0	39.0
70-71	25.543179529389498	32.0	10.5	36.0	2.0	39.0
72-73	24.79460882912725	31.5	6.5	35.5	2.0	38.5
74-75	24.071868755669414	31.0	2.0	35.0	2.0	38.0
76-77	23.151586986778135	29.5	2.0	34.0	2.0	36.0
78-79	23.166879904162073	30.0	2.0	34.0	2.0	36.0
80-81	22.61965408104624	30.0	2.0	34.0	2.0	36.0
82-83	21.894026912115862	29.0	2.0	34.0	2.0	35.5
84-85	21.156263185798903	29.0	2.0	34.0	2.0	35.0
86-87	20.49646866261531	28.0	2.0	34.0	2.0	35.0
88-89	19.863889057265375	26.5	2.0	33.0	2.0	35.0
90-91	19.118601245529113	26.0	2.0	33.0	2.0	34.5
92-93	18.233799868142505	24.5	2.0	32.0	2.0	34.0
94-95	17.253195204747975	21.5	2.0	32.0	2.0	34.0
96-97	16.298023208857224	12.5	2.0	31.0	2.0	34.0
98-99	15.423950485733666	2.0	2.0	31.0	2.0	34.0
100	14.635010784563134	2.0	2.0	31.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22774850085462361
1101	2	0.25955625082575295
1101	3	0.25062063103288423
1101	4	0.25894160347846906
1101	5	0.27029924277759676
1101	6	0.34951073864744586
1101	7	0.3532788331205552
1101	8	0.37576550581012924
1101	9	0.4329941617005346
1101	10-11	0.4466412439347067
1101	12-13	0.3920207238287219
1101	14-15	0.43418162749854616
1101	16-17	0.46391089451376644
1101	18-19	0.5005946936046541
1101	20-21	0.5068446371366448
1101	22-23	0.5379008253847672
1101	24-25	0.5101981413051746
1101	26-27	0.5360359304013258
1101	28-29	0.5591667639286086
1101	30-31	0.5565660338829019
1101	32-33	0.5281726535137707
1101	34-35	0.48292726951019915
1101	36-37	0.4113505222162459
1101	38-39	0.4047606001493449
1101	40-41	0.38422450691906107
1101	42-43	0.40135440988107973
1101	44-45	0.3609951501218518
1101	46-47	0.321002778070266
1101	48-49	0.35316672946622063
1101	50-51	0.2450804996681697
1101	52-53	0.233711145926371
1101	54-55	0.21708329407687188
1101	56-57	0.15986909259592608
1101	58-59	0.12284499686641226
1101	60-61	0.16665386361291112
1101	62-63	0.019608034795293605
1101	64-65	0.09512539344896709
1101	66-67	0.09889780975002083
1101	68-69	-0.06962346816090914
1101	70-71	-0.13482335498260412
1101	72-73	-0.13383449548933513
1101	74-75	-0.09739494050170094
1101	76-77	-0.07668112487939993
1101	78-79	-0.06994169466457834
1101	80-81	-0.08950549099656158
1101	82-83	-0.25127966457736406
1101	84-85	-0.2638102280342949
1101	86-87	-0.28667704488734813
1101	88-89	-0.35484527384351594
1101	90-91	-0.3860121044321012
1101	92-93	-0.396717980889143
1101	94-95	-0.2831409575780697
1101	96-97	-0.19472557706226468
1101	98-99	-0.0742862095614285
1101	100	0.019813668549938157
1102	1	0.23240195606862812
1102	2	0.2003774070804809
1102	3	0.19381447054019674
1102	4	0.14879931216384534
1102	5	0.186404278886954
1102	6	0.23394451088232415
1102	7	0.23504864924702673
1102	8	0.25671412520415515
1102	9	0.28729023947276744
1102	10-11	0.2993576025371709
1102	12-13	0.28056317210989334
1102	14-15	0.32708979813917693
1102	16-17	0.34868871257121725
1102	18-19	0.3829409725898927
1102	20-21	0.3788231621903293
1102	22-23	0.4433722160440752
1102	24-25	0.40253267601034537
1102	26-27	0.4333457621708021
1102	28-29	0.42042478835762864
1102	30-31	0.42165347727585356
1102	32-33	0.36567207176650385
1102	34-35	0.4217575432840803
1102	36-37	0.3697424929678448
1102	38-39	0.3367138331213937
1102	40-41	0.431380063402095
1102	42-43	0.40730599023788017
1102	44-45	0.3574103732730407
1102	46-47	0.3061827480685011
1102	48-49	0.4415155091317011
1102	50-51	0.333846943109009
1102	52-53	0.2902115948840134
1102	54-55	0.32714235218114496
1102	56-57	0.340033987014575
1102	58-59	0.2960005390375038
1102	60-61	0.3875618666940106
1102	62-63	0.3088097335868092
1102	64-65	0.3502396165716988
1102	66-67	0.3896420946812498
1102	68-69	0.30678372702385204
1102	70-71	0.32215688373315743
1102	72-73	0.30649192839563355
1102	74-75	0.348269527624268
1102	76-77	0.33913325709471565
1102	78-79	0.40906938671727033
1102	80-81	0.3588689324637109
1102	82-83	0.3324967612472456
1102	84-85	0.28362915006966816
1102	86-87	0.26494715947207936
1102	88-89	0.26678660876027394
1102	90-91	0.23210209082402145
1102	92-93	0.27936908835038565
1102	94-95	0.3406044277290121
1102	96-97	0.46926320338709004
1102	98-99	0.5427988322793116
1102	100	0.5587861258711175
1103	1	0.09739433294853939
1103	2	0.09334291800669448
1103	3	0.08064752617321602
1103	4	0.06752733837146963
1103	5	0.1208321356957498
1103	6	0.1259272604580275
1103	7	0.10097828055934599
1103	8	0.11045164602256108
1103	9	0.15291868524393237
1103	10-11	0.15581717374669068
1103	12-13	0.12787322158260395
1103	14-15	0.1823777125304531
1103	16-17	0.22376149962011027
1103	18-19	0.24527203469627068
1103	20-21	0.1859418155570438
1103	22-23	0.29285194885292753
1103	24-25	0.25618459856998044
1103	26-27	0.26419047475000923
1103	28-29	0.2995445759989295
1103	30-31	0.18179491209779997
1103	32-33	0.1873718651501619
1103	34-35	0.20231286004191418
1103	36-37	0.24458981254384327
1103	38-39	0.23473509327010333
1103	40-41	0.3779374410000358
1103	42-43	0.36790530833725654
1103	44-45	0.2719699062597982
1103	46-47	0.3052117453678136
1103	48-49	0.42414112466422793
1103	50-51	0.3428222704660726
1103	52-53	0.2909034659322174
1103	54-55	0.3284910727911736
1103	56-57	0.3482107675957131
1103	58-59	0.2839629137237907
1103	60-61	0.3093124020028313
1103	62-63	0.3377527762057184
1103	64-65	0.4569403262625862
1103	66-67	0.45223073076799736
1103	68-69	0.3794129186193693
1103	70-71	0.4377649229910716
1103	72-73	0.46150195743485867
1103	74-75	0.4021164274186759
1103	76-77	0.46833963033682835
1103	78-79	0.5058904834301607
1103	80-81	0.4592529622671577
1103	82-83	0.3346035875976128
1103	84-85	0.1718669262449879
1103	86-87	0.24574141925180015
1103	88-89	0.39161119888827756
1103	90-91	0.36443317083217863
1103	92-93	0.4332063623812701
1103	94-95	0.6124412649831683
1103	96-97	0.6846117475811759
1103	98-99	0.7343162536141588
1103	100	0.6743154121890438
1104	1	0.026698454532251503
1104	2	0.02384239661810028
1104	3	0.007369489506498184
1104	4	-0.027705055152090097
1104	5	-0.057932363955316646
1104	6	-0.008842435216273259
1104	7	-0.021446296355037475
1104	8	0.024620714707900504
1104	9	0.040565889261465315
1104	10-11	0.043917129544453815
1104	12-13	0.03703131048501973
1104	14-15	-0.021019255941226334
1104	16-17	0.08329049169397251
1104	18-19	0.08887363458062225
1104	20-21	0.10073485160725681
1104	22-23	0.15077414572939318
1104	24-25	0.1130756033625957
1104	26-27	0.10913994060015852
1104	28-29	0.07411417190647285
1104	30-31	0.07240945080165773
1104	32-33	0.16366889447569832
1104	34-35	0.19934167139449244
1104	36-37	0.1262491821129501
1104	38-39	0.0818462089498837
1104	40-41	0.11187093548458193
1104	42-43	0.10894302068574291
1104	44-45	0.07449588796445994
1104	46-47	0.0923459727831144
1104	48-49	0.1520566622053927
1104	50-51	0.10344968691467216
1104	52-53	0.007950754385390724
1104	54-55	0.11418584747642413
1104	56-57	0.11217195825154036
1104	58-59	0.14381116211715295
1104	60-61	0.16144908353985699
1104	62-63	0.0052356003352933556
1104	64-65	0.19167618519666618
1104	66-67	0.27259449296267135
1104	68-69	0.2056154350345203
1104	70-71	0.3176502176713569
1104	72-73	0.33187738201237593
1104	74-75	0.31168564268433485
1104	76-77	0.3346990122500415
1104	78-79	0.39470129959168077
1104	80-81	0.34296425821409215
1104	82-83	0.3213365137543711
1104	84-85	0.2832115344120396
1104	86-87	0.32668533193227134
1104	88-89	0.39516327527130457
1104	90-91	0.41814723730064784
1104	92-93	0.5278511562033152
1104	94-95	0.6021334083263454
1104	96-97	0.676537737219423
1104	98-99	0.7089196513827272
1104	100	0.65400432019349
1105	1	-0.13415227526597562
1105	2	-0.14659364175066614
1105	3	-0.14319037462500006
1105	4	-0.14543097094150426
1105	5	-0.15473761432419053
1105	6	-0.25519360216973297
1105	7	-0.17275214431228392
1105	8	-0.18473901000152182
1105	9	-0.1568733434693783
1105	10-11	-0.13477421864487837
1105	12-13	-0.27698563761055084
1105	14-15	-0.28968698940981596
1105	16-17	-0.20634376781919883
1105	18-19	-0.36384572878122867
1105	20-21	-0.2294899673530324
1105	22-23	-0.26726271529938117
1105	24-25	-0.33581382649666836
1105	26-27	-0.19240852329297553
1105	28-29	-0.2539162164189577
1105	30-31	-0.1468124581477923
1105	32-33	-0.23852484416196518
1105	34-35	-0.1309146713809568
1105	36-37	-0.11675374824225315
1105	38-39	-0.202159701248263
1105	40-41	-0.20146571883306308
1105	42-43	-0.09993520586527183
1105	44-45	-0.13301593319521032
1105	46-47	-0.1109003179921757
1105	48-49	-0.11080997107978519
1105	50-51	-0.12364517178189516
1105	52-53	-0.12308542948889212
1105	54-55	-0.022816210510612223
1105	56-57	-0.045204408069160706
1105	58-59	-0.05630508799264078
1105	60-61	0.016906844251902697
1105	62-63	-0.007255906776709509
1105	64-65	-0.007209886308018554
1105	66-67	0.09482571887754432
1105	68-69	-0.007832461898804155
1105	70-71	0.053526633800494494
1105	72-73	0.13169285364067562
1105	74-75	0.12108101609040745
1105	76-77	0.24854491778886256
1105	78-79	0.18303979885878974
1105	80-81	0.018267751610206773
1105	82-83	0.27300260497627704
1105	84-85	0.23381829174304514
1105	86-87	0.41828589156106943
1105	88-89	0.426200501658343
1105	90-91	0.3651567067945436
1105	92-93	0.44632543896289434
1105	94-95	0.46252505037689673
1105	96-97	0.5832194932795858
1105	98-99	0.7095961689879271
1105	100	0.7223484121711561
1106	1	-0.18493713987797022
1106	2	-0.1508762085538713
1106	3	-0.18409838553212055
1106	4	-0.15850389750843163
1106	5	-0.19734105383979994
1106	6	-0.15807558553220247
1106	7	-0.14608419005057982
1106	8	-0.19328930713746217
1106	9	-0.20073445051683336
1106	10-11	-0.1879937741443456
1106	12-13	-0.23923876725919513
1106	14-15	-0.21531873738810248
1106	16-17	-0.24078964407411263
1106	18-19	-0.2759073667211567
1106	20-21	-0.26547674047072434
1106	22-23	-0.2914484885604054
1106	24-25	-0.3118316771284739
1106	26-27	-0.3843829095309985
1106	28-29	-0.3175892780154328
1106	30-31	-0.3847933360273146
1106	32-33	-0.4232312009165611
1106	34-35	-0.3304394155763717
1106	36-37	-0.30623186534270985
1106	38-39	-0.3789399224646033
1106	40-41	-0.3065683642670436
1106	42-43	-0.3771221449328195
1106	44-45	-0.255884133536469
1106	46-47	-0.3717549711187047
1106	48-49	-0.30630842035252215
1106	50-51	-0.33814643083993445
1106	52-53	-0.32397357409031
1106	54-55	-0.2644175133337363
1106	56-57	-0.43387268738268503
1106	58-59	-0.339693786270832
1106	60-61	-0.4007032769904093
1106	62-63	-0.307167422424552
1106	64-65	-0.2501813265490931
1106	66-67	-0.258906984045435
1106	68-69	-0.3209435491287316
1106	70-71	-0.23362348333450456
1106	72-73	-0.2797471206399287
1106	74-75	-0.2918434575738189
1106	76-77	-0.1498793576336439
1106	78-79	-0.18327190950937222
1106	80-81	-0.242688585163652
1106	82-83	-0.18537810438875013
1106	84-85	-0.2875669033839614
1106	86-87	-0.15428224137667002
1106	88-89	-0.22924198755652725
1106	90-91	-0.3226007795938486
1106	92-93	-0.2709144848582632
1106	94-95	-0.20793079954739468
1106	96-97	-0.024387479551233326
1106	98-99	0.07754549414500467
1106	100	0.04266184190551492
1107	1	-0.42758264314541705
1107	2	-0.3695339557078192
1107	3	-0.34223458065917356
1107	4	-0.3413125485227937
1107	5	-0.3444739933399603
1107	6	-0.3339009673639026
1107	7	-0.35290739980364805
1107	8	-0.3930042193895886
1107	9	-0.39339519234956555
1107	10-11	-0.3892251101325144
1107	12-13	-0.40647236849579826
1107	14-15	-0.4982930343523293
1107	16-17	-0.5601007290863862
1107	18-19	-0.4908504264217868
1107	20-21	-0.5896837718679535
1107	22-23	-0.5188105389233044
1107	24-25	-0.5131500898934931
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2208	1	-0.30531319813473345
2208	2	-0.2654041678035277
2208	3	-0.2274468382858572
2208	4	-0.24438380369542756
2208	5	-0.31111332873034314
2208	6	-0.26677767056746404
2208	7	-0.22446846593687297
2208	8	-0.2547286241925306
2208	9	-0.27938707900255366
2208	10-11	-0.22758259695155658
2208	12-13	-0.3368879344922746
2208	14-15	-0.5499373770940252
2208	16-17	-0.45928557278429594
2208	18-19	-0.557023379154721
2208	20-21	-0.5238344607251335
2208	22-23	-0.5918933928127359
2208	24-25	-0.5949016163866432
2208	26-27	-0.7818257326246894
2208	28-29	-0.606940688068228
2208	30-31	-0.6732342973856547
2208	32-33	-0.5266593029614697
2208	34-35	-0.6050145459222875
2208	36-37	-0.6505909506448759
2208	38-39	-0.610219256834327
2208	40-41	-0.9076881915366855
2208	42-43	-0.46829527391794556
2208	44-45	-0.3514435612178204
2208	46-47	-0.20943802939033418
2208	48-49	-0.49270563751208485
2208	50-51	-0.760532590236437
2208	52-53	-0.3062639208239162
2208	54-55	-0.16607123336776652
2208	56-57	-0.10290092468290979
2208	58-59	-0.7216397072012413
2208	60-61	-0.39812045322689116
2208	62-63	-0.5531847022051757
2208	64-65	-0.31307975932749343
2208	66-67	-0.5027820776299272
2208	68-69	-0.31611711317580315
2208	70-71	-0.35956644093284496
2208	72-73	-0.43448318718241197
2208	74-75	-0.9250582180197569
2208	76-77	-0.7273190281369821
2208	78-79	-1.2072175417589257
2208	80-81	-1.0012864338481045
2208	82-83	-0.8283820064594174
2208	84-85	-1.0704341656456293
2208	86-87	-1.4072426907580002
2208	88-89	-1.6507225700900037
2208	90-91	-1.515402683562197
2208	92-93	-1.9803286849582058
2208	94-95	-2.346048330777986
2208	96-97	-1.9684067745725216
2208	98-99	-2.245999054554753
2208	100	-2.357579544026583
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	442643.0
3	182645.0
4	163122.0
5	136444.0
6	123615.0
7	126704.0
8	131566.0
9	148647.0
10	169169.0
11	188788.0
12	208527.0
13	240551.0
14	282977.0
15	348593.0
16	410005.0
17	414835.0
18	389768.0
19	374109.0
20	379970.0
21	395281.0
22	414246.0
23	436013.0
24	461094.0
25	490234.0
26	528532.0
27	575847.0
28	640648.0
29	725805.0
30	831439.0
31	963002.0
32	1140824.0
33	1390373.0
34	1780975.0
35	2418605.0
36	3177901.0
37	3012044.0
38	1100138.0
39	43846.0
40	6.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.555635419338063	28.735280077813062	28.338192440533238	22.370892062315637
2	23.35955459074058	28.544411028824555	27.622763804187933	20.47327057624693
3	22.77760449679959	26.9072304670839	27.503183553842188	22.811981482274327
4	23.516920395328764	26.55342418875273	27.164251039137493	22.765404376781017
5	23.81898573972369	26.786186383299277	27.076281520747898	22.318546356229138
6	23.863170880033273	26.59741762587321	26.4446154718729	23.094796022220613
7	23.902693771619415	26.986796058942	26.55269922909555	22.557810940343032
8	23.21615333658676	26.752454064566532	27.03210994700857	22.999282651838136
9	23.274044357219758	26.41085164743176	27.11510651649559	23.199997478852893
10-11	23.258030531345938	26.695727070891063	26.883172192446597	23.1630702053164
12-13	23.029459423478524	26.735939283511406	27.017596721706642	23.21700457130343
14-15	22.790589278344115	26.96133380569948	27.412043272709596	22.836033643246807
16-17	22.6302129151764	26.89362102871814	27.52510276424728	22.95106329185818
18-19	22.329101101697866	26.813017087999235	28.09033167837865	22.76755013192425
20-21	22.090229552452037	26.608140173016526	28.782010911042118	22.519619363489323
22-23	22.071257171512848	26.579205088009022	29.08040864931754	22.26912909116059
24-25	22.049962418197318	26.44017243491813	29.394279581471682	22.115585565412868
26-27	22.096671594150376	26.46554041337179	29.434772264527236	22.003015727950594
28-29	22.138819311336828	26.554529687366763	29.373939884601153	21.93271111669526
30-31	22.150581388192023	26.47925097800745	29.325048069466224	22.045119564334303
32-33	22.1567807764087	26.524306459298973	29.291103087757055	22.027809676535274
34-35	22.21238092743415	26.60994814150588	29.247072016757947	21.93059891430202
36-37	22.21870973803789	26.547391132678534	29.187816654076144	22.046082475207427
38-39	22.268603752860784	26.583494229053233	29.100770024789135	22.047131993296844
40-41	22.335074706716227	26.710444457000726	28.88826243151194	22.066218404771103
42-43	22.485236710774945	26.774520670298557	28.51131694461726	22.228925674309238
44-45	22.572293095292615	26.917881438758506	28.168729188323006	22.341096277625873
46-47	22.73964331750688	27.10047985106837	27.78519304999383	22.374683781430925
48-49	22.84259247515024	27.101640279821382	27.480510908715196	22.575256336313178
50-51	22.90554086130518	27.159280496711922	27.32064262674088	22.61453601524202
52-53	22.99215185263568	27.260611218558	27.15719959414522	22.590037334661105
54-55	23.01175200086375	27.187649966551664	27.125562854023478	22.67503517856111
56-57	22.997265225254083	27.2338638305036	27.09659925830889	22.672271685933428
58-59	23.039327762935045	27.30843726172426	27.044340471466782	22.60789450387391
60-61	23.013848215282877	27.27145613939487	26.970803565859853	22.743892079462398
62-63	23.05638755123171	27.331489266264498	26.930375925148514	22.68174725735528
64-65	23.099834804978766	27.44697020813612	26.823889676085173	22.629305310799943
66-67	23.128961633439026	27.42476013070953	26.723635253886822	22.722642981964615
68-69	23.144078586955704	27.478083509227474	26.652487853618524	22.7253500501983
70-71	23.214697641556555	27.598141503455302	26.50221209446379	22.68494876052435
72-73	23.229979407075017	27.590056626604735	26.394514829426928	22.78544913689332
74-75	23.299276988384356	27.634347132548665	26.35352365748585	22.71285222158113
76-77	22.863783608636908	28.010084378391355	26.148546902813614	22.977585110158127
78-79	23.017236580381397	27.965873294057687	26.047372831205234	22.969517294355683
80-81	22.986568252345258	27.99087930161041	25.992332087201326	23.030220358843003
82-83	22.95082009184096	28.16194147034906	25.843281122828582	23.043957314981395
84-85	22.944119781897097	28.157655750459003	25.71875899784976	23.17946546979414
86-87	22.8837304218007	28.22484008216789	25.677468942935477	23.213960553095934
88-89	22.87577330326668	28.363061947099954	25.60197542737816	23.159189322255198
90-91	22.804890445249097	28.377029921194147	25.432949044071506	23.385130589485247
92-93	22.767636985866353	28.413732126107945	25.372407687084454	23.44622320094125
94-95	22.75620310535851	28.541994773658118	25.137538577701612	23.564263543281765
96-97	22.699310794160205	28.585769799628707	25.032104440064	23.682814966147088
98-99	22.67387837575207	28.6136575640346	24.900047668936985	23.81241639127634
100	23.225877222782515	28.651978523351413	24.253515518144926	23.868628735721146
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4821.0
1	3457.5
2	1823.5
3	1598.0
4	1654.5
5	1808.0
6	2092.5
7	2391.5
8	2799.5
9	3258.0
10	3798.5
11	4478.5
12	5515.5
13	7235.5
14	11158.0
15	15538.0
16	21153.0
17	23646.5
18	23924.5
19	28740.0
20	39232.0
21	49344.0
22	59358.5
23	76185.0
24	96117.5
25	118203.0
26	145379.0
27	181482.5
28	222497.5
29	263921.0
30	303961.5
31	346227.0
32	394547.0
33	446764.5
34	501738.0
35	559669.0
36	622822.0
37	692401.0
38	761223.0
39	817021.5
40	861774.5
41	902635.0
42	947121.0
43	982869.5
44	994262.5
45	994376.0
46	989455.5
47	970465.5
48	940126.0
49	908627.5
50	867238.5
51	818037.5
52	765478.0
53	709083.5
54	659663.5
55	611724.5
56	558320.0
57	505463.5
58	458482.0
59	415999.0
60	372360.0
61	329845.5
62	291857.5
63	257056.0
64	224800.0
65	195147.0
66	168034.0
67	143007.0
68	120896.5
69	101826.0
70	85234.5
71	70882.0
72	58776.5
73	48769.5
74	40492.5
75	33249.0
76	26783.0
77	21412.5
78	16938.5
79	13270.5
80	10294.0
81	7930.5
82	6084.5
83	4611.5
84	3477.0
85	2511.0
86	1804.5
87	1352.0
88	943.0
89	669.0
90	486.5
91	335.0
92	230.5
93	164.0
94	107.0
95	57.0
96	31.5
97	17.5
98	6.0
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18814841439962007
2	8.152966669608823E-4
3	0.0017802613210933277
4	0.006494802916997561
5	0.020087019330920292
6	0.0019338679395062477
7	0.009208519842292479
8	0.01171348931179548
9	0.016782507719421837
10-11	0.004070575387942377
12-13	0.0028003668126047702
14-15	0.009629953385117669
16-17	0.0023395469573660108
18-19	0.0028318758625356256
20-21	0.018858166383616932
22-23	0.01344057911113049
24-25	0.011459447596727958
26-27	0.0011993132129931821
28-29	7.719717233059563E-4
30-31	0.012184155745137632
32-33	0.012696177806514032
34-35	0.008570461581192658
36-37	0.0028574769656044456
38-39	0.004696817755318127
40-41	0.006378613295377532
42-43	0.001797985161679434
44-45	0.003897275613322672
46-47	0.004872086845558511
48-49	0.010890315382351884
50-51	0.005096588826315854
52-53	0.014192857678229661
54-55	0.01031921385235513
56-57	0.005254134075970131
58-59	0.008107672410333219
60-61	0.002329700379262618
62-63	0.005691322143760749
64-65	0.007727594495542277
66-67	0.005234440919763346
68-69	0.018541106568687703
70-71	0.014316924562332405
72-73	0.008550768424985873
74-75	0.009401512773118967
76-77	0.00923215162974062
78-79	0.026436092891987645
80-81	0.009610260228910884
82-83	0.004669247336628628
84-85	0.012830091268720166
86-87	0.004033158391149485
88-89	0.011114817363109228
90-91	0.01442523692146972
92-93	0.0031095493650512883
94-95	0.0032926957177743846
96-97	0.006646440219789803
98-99	0.007597619664577499
100	0.0040410356536322
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.5389531E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.75906112062555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.49000038050782	41.410587274331355
2	11.437834864203793	12.068988573762997
3	3.9461522363678365	6.245858610894716
4	1.7703625274205528	3.7361065915938427
5	1.0000412645087613	2.63806190986827
6	0.6149023933389259	1.9465003772032394
7	0.4260896886198192	1.5736064347332948
8	0.2977213748457014	1.2566000169920823
9	0.23991819799461492	1.139207298475341
>10	1.5634955723185615	16.11782033612
>50	0.14229942074235993	5.219556157780426
>100	0.07067785778998249	5.70441174223157
>500	2.4931629782401433E-4	0.08940557862582628
>1k	1.2783946580933957E-4	0.12719395826237323
>5k	8.969334893146704E-5	0.35074992886328166
>10k+	3.7372228717693304E-5	0.3753452102613315
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32744	0.12896654136699098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	7.87726248271384E-6	0.0	7.87726248271384E-6	3.93863124135692E-6
2	0.0	1.1815893724070759E-5	0.0	1.1815893724070759E-5	3.93863124135692E-6
3	0.0	1.1815893724070759E-5	0.0	1.1815893724070759E-5	3.93863124135692E-6
4	0.0	1.1815893724070759E-5	0.0	1.1815893724070759E-5	3.93863124135692E-6
5	0.0	1.1815893724070759E-5	0.0	2.3631787448141518E-5	7.87726248271384E-6
6	0.0	1.1815893724070759E-5	0.0	2.3631787448141518E-5	1.96931562067846E-5
7	0.0	1.1815893724070759E-5	0.0	2.3631787448141518E-5	2.3631787448141518E-5
8	3.93863124135692E-6	1.1815893724070759E-5	0.0	2.3631787448141518E-5	2.3631787448141518E-5
9	1.575452496542768E-5	1.575452496542768E-5	0.0	2.7570418689498438E-5	2.3631787448141518E-5
10-11	3.544768117221228E-5	1.575452496542768E-5	0.0	2.7570418689498438E-5	3.150904993085536E-5
12-13	1.4376004030952757E-4	1.575452496542768E-5	0.0	3.150904993085536E-5	3.150904993085536E-5
14-15	4.096176491011197E-4	1.575452496542768E-5	0.0	4.726357489628304E-5	3.150904993085536E-5
16-17	9.393635510636254E-4	2.560110306881998E-5	0.0	5.7110152999675335E-5	3.150904993085536E-5
18-19	0.0016975500650248325	3.347836555153382E-5	0.0	5.90794686203538E-5	3.150904993085536E-5
20-21	0.002585711409950818	3.93863124135692E-5	0.0	6.301809986171072E-5	3.150904993085536E-5
22-23	0.003859858616529781	4.92328905169615E-5	0.0	6.695673110306764E-5	4.135562803424766E-5
24-25	0.005325029438314555	5.5140837378996876E-5	0.0	6.89260467237461E-5	4.332494365492612E-5
26-27	0.007010763609615318	5.90794686203538E-5	0.0	7.483399358578147E-5	4.7263574896283036E-5
28-29	0.00879693287757068	6.104878424103226E-5	0.0	7.87726248271384E-5	5.120220613763996E-5
30-31	0.01025619575249342	6.695673110306764E-5	3.93863124135692E-6	7.87726248271384E-5	5.90794686203538E-5
32-33	0.011626839424485627	6.89260467237461E-5	3.93863124135692E-6	8.271125606849532E-5	5.90794686203538E-5
34-35	0.013092010246270401	7.089536234442456E-5	3.93863124135692E-6	8.664988730985224E-5	5.90794686203538E-5
36-37	0.014622168483537566	7.680330920645993E-5	3.93863124135692E-6	9.452714979256609E-5	5.90794686203538E-5
38-39	0.016103093830287768	7.87726248271384E-5	3.93863124135692E-6	1.0437372789595838E-4	5.90794686203538E-5
40-41	0.017603712333244752	8.271125606849532E-5	3.93863124135692E-6	1.1028167475799375E-4	6.89260467237461E-5
42-43	0.019275661295200766	8.664988730985224E-5	3.93863124135692E-6	1.1028167475799375E-4	7.483399358578147E-5
44-45	0.02122725307529312	8.664988730985224E-5	3.93863124135692E-6	1.1225099037867221E-4	7.483399358578147E-5
46-47	0.023499843301556064	9.649646541324454E-5	3.93863124135692E-6	1.1618962162002914E-4	7.877262482713839E-5
48-49	0.02611312513019638	1.0240441227527992E-4	3.93863124135692E-6	1.2012825286138607E-4	8.271125606849532E-5
50-51	0.029177380235972064	1.0634304351663684E-4	3.93863124135692E-6	1.2209756848206453E-4	8.468057168917378E-5
52-53	0.03279895166239975	1.0634304351663684E-4	3.93863124135692E-6	1.2603619972342145E-4	9.649646541324454E-5
54-55	0.03717083234030594	1.0634304351663684E-4	3.93863124135692E-6	1.378520934474922E-4	9.8465781033923E-5
56-57	0.04274399554682597	1.0634304351663684E-4	5.90794686203538E-6	1.4179072468884911E-4	9.8465781033923E-5
58-59	0.04918168831082386	1.083123591373153E-4	7.87726248271384E-6	1.4572935593020603E-4	9.8465781033923E-5
60-61	0.056972300906227846	1.1422030599935068E-4	7.87726248271384E-6	1.476986715508845E-4	1.0043509665460146E-4
62-63	0.06627140926707153	1.1422030599935068E-4	7.87726248271384E-6	1.516373027922414E-4	1.0240441227527992E-4
64-65	0.0776304217671449	1.181589372407076E-4	7.87726248271384E-6	1.5360661841291986E-4	1.0437372789595838E-4
66-67	0.09140578453379072	1.181589372407076E-4	7.87726248271384E-6	1.575452496542768E-4	1.1815893724070761E-4
68-69	0.1087416699426232	1.2012825286138607E-4	7.87726248271384E-6	1.673918277576691E-4	1.2209756848206453E-4
70-71	0.1288365665360262	1.2209756848206453E-4	7.87726248271384E-6	1.772384058610614E-4	1.2209756848206453E-4
72-73	0.15356526278488564	1.24066884102743E-4	7.87726248271384E-6	1.772384058610614E-4	1.24066884102743E-4
74-75	0.18282141564568483	1.280055153440999E-4	7.87726248271384E-6	1.7920772148173985E-4	1.2997483096477836E-4
76-77	0.21714264828286903	1.2997483096477836E-4	7.87726248271384E-6	1.9102361520581063E-4	1.3391346220613528E-4
78-79	0.2588310906570114	1.2997483096477836E-4	7.87726248271384E-6	2.0087019330920292E-4	1.3391346220613528E-4
80-81	0.30759725337187205	1.3391346220613528E-4	7.87726248271384E-6	2.0874745579191675E-4	1.3588277782681374E-4
82-83	0.36339584216817555	1.3391346220613528E-4	7.87726248271384E-6	2.1268608703327367E-4	1.4376004030952757E-4
84-85	0.42913750553328456	1.3391346220613528E-4	7.87726248271384E-6	2.1859403389530905E-4	1.4572935593020603E-4
86-87	0.5051609657539559	1.378520934474922E-4	7.87726248271384E-6	2.2450198075734442E-4	1.4966798717156295E-4
88	0.5680805998346327	1.4966798717156295E-4	7.87726248271384E-6	2.363178744814152E-4	1.5360661841291986E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	16145	0.0	39.8383	1
TCTCGAG	18235	0.0	34.742798	2
GATCCGG	24380	0.0	27.98484	1
CTCGAGG	23260	0.0	27.21904	3
TCGAGGG	26125	0.0	24.071808	4
ATCCGGC	28670	0.0	23.851515	2
TCCGGCG	32560	0.0	20.93141	3
TATCTCG	9820	0.0	20.196754	2
GCGCCGT	35665	0.0	18.869448	9
GGATCCG	16560	0.0	17.085072	2
CGGCGCG	39590	0.0	17.059473	5
CGAGGGC	38120	0.0	16.49672	5
CCGGCGC	42050	0.0	16.207308	4
CGTCGAC	34085	0.0	15.897183	12-13
GCCGTCG	35185	0.0	15.613859	10-11
AGGGCGC	41090	0.0	15.166504	7
TCGACTT	36605	0.0	14.868752	14-15
TATGGAT	15485	0.0	14.200179	1
GAGGGCG	45125	0.0	13.945765	6
TGGATCC	21660	0.0	13.782213	1
>>END_MODULE