FastQCFastQC Report
Wed 25 May 2016
SRR491023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23093630
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295630.12801365571371845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA160250.047.3973271
TCTCGAG192750.039.2795642
CTCGAGG245800.030.8808363
GATCCGG252650.030.5662421
TCGAGGG302600.025.0064814
TATCTCG88200.024.9902022
ATCCGGC311450.024.7016432
TCCGGCG415400.018.7366643
ATATCTC121300.018.439551
GGATCCG164650.018.2391512
GCGCCGT433150.017.7717349
CGAGGGC443500.017.1887535
CGGCGCG451150.016.9910475
ATGGATC168950.016.8343451
AGGGCGC480300.015.6361797
CGTCGAC414250.015.55299812-13
CCGGCGC500500.015.4568624
TGGATCC201750.015.4511911
TCGACTT431000.014.99423514-15
GCCGTCG443600.014.70944610-11
Produced by FastQC (version 0.11.5)