##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491023_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23093630
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.396539002313624	33.0	31.0	34.0	28.0	34.0
2	31.671768318796136	34.0	31.0	34.0	28.0	34.0
3	31.75021484279431	34.0	31.0	34.0	28.0	34.0
4	35.128933866178684	37.0	35.0	37.0	32.0	37.0
5	34.88086394386677	37.0	35.0	37.0	32.0	37.0
6	34.76906129525761	37.0	35.0	37.0	32.0	37.0
7	34.72074684664126	37.0	35.0	37.0	32.0	37.0
8	34.606496250264684	37.0	35.0	37.0	32.0	37.0
9	36.08566396880872	39.0	37.0	39.0	31.0	39.0
10-11	35.91614287143251	39.0	37.0	39.0	30.5	39.0
12-13	35.797454449560334	39.0	37.0	39.0	30.0	39.0
14-15	37.019463245925394	40.0	38.0	41.0	31.0	41.0
16-17	36.90575197576128	40.0	38.0	41.0	30.0	41.0
18-19	36.75041236479497	40.0	37.0	41.0	30.0	41.0
20-21	36.63109965821744	40.0	37.0	41.0	30.0	41.0
22-23	36.40885752911084	40.0	37.0	41.0	29.0	41.0
24-25	36.21171710553949	40.0	37.0	41.0	27.5	41.0
26-27	35.84165224349745	40.0	36.0	41.0	27.0	41.0
28-29	35.5035529494497	39.5	35.5	41.0	25.5	41.0
30-31	35.19378843863005	39.0	35.0	41.0	24.5	41.0
32-33	34.96258977042587	39.0	35.0	41.0	24.0	41.0
34-35	34.686505759380395	39.0	35.0	40.5	22.5	41.0
36-37	34.42250828475211	39.0	34.5	40.0	19.5	41.0
38-39	34.05917198811967	38.5	34.0	40.0	15.5	41.0
40-41	33.602967030302295	38.0	33.0	40.0	10.5	41.0
42-43	33.12727317013393	38.0	33.0	40.0	5.0	41.0
44-45	32.6307866065231	38.0	32.5	40.0	2.0	41.0
46-47	32.090787243062266	38.0	31.5	40.0	2.0	41.0
48-49	31.542445838960788	37.5	31.0	40.0	2.0	41.0
50-51	30.99561658344747	37.0	30.0	40.0	2.0	41.0
52-53	30.437246613027057	36.5	28.5	40.0	2.0	41.0
54-55	29.88147225013997	36.0	28.0	39.0	2.0	40.0
56-57	29.351803462686462	35.5	26.5	39.0	2.0	40.0
58-59	28.808809139143563	35.0	26.0	39.0	2.0	40.0
60-61	28.20398503829844	34.5	24.0	38.0	2.0	40.0
62-63	27.551365874485732	34.0	22.5	38.0	2.0	40.0
64-65	26.88505949476111	34.0	19.5	37.5	2.0	39.5
66-67	26.20465240847801	33.0	15.5	36.5	2.0	39.0
68-69	25.47995997164586	32.0	8.0	36.0	2.0	39.0
70-71	24.730801026083817	31.5	4.5	35.5	2.0	38.5
72-73	23.9666895156803	31.0	2.0	35.0	2.0	37.5
74-75	23.23241978415693	30.5	2.0	34.5	2.0	37.0
76-77	22.344258957989716	29.0	2.0	34.0	2.0	36.0
78-79	22.33547660112334	30.0	2.0	34.0	2.0	36.0
80-81	21.794281150256587	29.0	2.0	34.0	2.0	35.5
82-83	21.083882633436147	29.0	2.0	34.0	2.0	35.0
84-85	20.360910129763056	27.5	2.0	33.5	2.0	35.0
86-87	19.713972814148317	26.5	2.0	33.0	2.0	35.0
88-89	19.097037862821914	25.5	2.0	33.0	2.0	34.5
90-91	18.35932168307884	25.0	2.0	32.0	2.0	34.0
92-93	17.476756469208176	21.5	2.0	32.0	2.0	34.0
94-95	16.499738152901905	17.5	2.0	31.5	2.0	34.0
96-97	15.546652799927946	4.5	2.0	31.0	2.0	34.0
98-99	14.672696345269237	2.0	2.0	31.0	2.0	34.0
100	13.869687745062166	2.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20188806702248385
1101	2	0.2355200843117622
1101	3	0.23259635524798483
1101	4	0.19921941584757263
1101	5	0.2454048680519918
1101	6	0.2919937117343636
1101	7	0.30082703309400927
1101	8	0.33004316411111034
1101	9	0.38057466370153037
1101	10-11	0.3855800832291081
1101	12-13	0.34715265435272613
1101	14-15	0.4069597639850713
1101	16-17	0.4078455251320747
1101	18-19	0.470936306462697
1101	20-21	0.4582958913365047
1101	22-23	0.48816223524406865
1101	24-25	0.4693835573239937
1101	26-27	0.4705861033936003
1101	28-29	0.5037503574989657
1101	30-31	0.50468540805808
1101	32-33	0.4920156290512523
1101	34-35	0.4393367254191034
1101	36-37	0.3639570907564291
1101	38-39	0.2977565889223257
1101	40-41	0.2698255952920192
1101	42-43	0.2656414874030091
1101	44-45	0.24800529346153155
1101	46-47	0.20576338502849723
1101	48-49	0.2727374944262664
1101	50-51	0.1534782982515317
1101	52-53	0.1692476739159936
1101	54-55	0.13044425754529954
1101	56-57	0.028655891064872208
1101	58-59	0.04897736514050166
1101	60-61	0.04835153320351182
1101	62-63	-0.07206470358381623
1101	64-65	-0.03886501454107716
1101	66-67	-0.025336164963139396
1101	68-69	-0.2217238750573216
1101	70-71	-0.2497075975868377
1101	72-73	-0.2392125769490292
1101	74-75	-0.23305406603878964
1101	76-77	-0.27518728660833247
1101	78-79	-0.24248385574910003
1101	80-81	-0.25362887765478703
1101	82-83	-0.41572179399027576
1101	84-85	-0.46581743633002404
1101	86-87	-0.46902622271714023
1101	88-89	-0.5136917521051387
1101	90-91	-0.5036307590460964
1101	92-93	-0.5266688118702199
1101	94-95	-0.4467097561137692
1101	96-97	-0.3537894542914035
1101	98-99	-0.23301705019470909
1101	100	-0.10224489890752686
1102	1	0.19807771315381828
1102	2	0.18014975293834468
1102	3	0.16388262779002716
1102	4	0.12056574193188396
1102	5	0.17928042745948147
1102	6	0.2243087595862434
1102	7	0.2088439809068987
1102	8	0.2210335951142568
1102	9	0.2378974565853511
1102	10-11	0.2516125765966706
1102	12-13	0.22430041382955324
1102	14-15	0.29301799752308
1102	16-17	0.3060564086956248
1102	18-19	0.33500041374350076
1102	20-21	0.34028046901141806
1102	22-23	0.4001423519818914
1102	24-25	0.3519079675111243
1102	26-27	0.38711237962746736
1102	28-29	0.3729382490165989
1102	30-31	0.38526333673728175
1102	32-33	0.31190641143996345
1102	34-35	0.33922931109018606
1102	36-37	0.3161737596450678
1102	38-39	0.28533123934681726
1102	40-41	0.3617294333792813
1102	42-43	0.35280290434715056
1102	44-45	0.28733951495108556
1102	46-47	0.27208365850127336
1102	48-49	0.3764191872081213
1102	50-51	0.2785538319515446
1102	52-53	0.25700762729389126
1102	54-55	0.27138357230052534
1102	56-57	0.2969588040775797
1102	58-59	0.29253792653480204
1102	60-61	0.3510299171301483
1102	62-63	0.25651336271046077
1102	64-65	0.2599995885618469
1102	66-67	0.3346219465818976
1102	68-69	0.26706336798067554
1102	70-71	0.24136953385726656
1102	72-73	0.25697319370266314
1102	74-75	0.27454777720809176
1102	76-77	0.27006876837769767
1102	78-79	0.31217481788006296
1102	80-81	0.27956558007040044
1102	82-83	0.26570951437465595
1102	84-85	0.25078234295699176
1102	86-87	0.21627633723426243
1102	88-89	0.18723159428411051
1102	90-91	0.19657940995212897
1102	92-93	0.1773671663595202
1102	94-95	0.26057927565349814
1102	96-97	0.38839684521689044
1102	98-99	0.4113492628643076
1102	100	0.44077743442085193
1103	1	0.10937481987924969
1103	2	0.1251452460149558
1103	3	0.08622903853557062
1103	4	0.09472086520798229
1103	5	0.12433372442619373
1103	6	0.13595733783428443
1103	7	0.12027457398696129
1103	8	0.13172003783652286
1103	9	0.1700517237910404
1103	10-11	0.16035075295793177
1103	12-13	0.12985652475639142
1103	14-15	0.15894749941542585
1103	16-17	0.1858494843352645
1103	18-19	0.21285124805119437
1103	20-21	0.1583582391563283
1103	22-23	0.2470069622601372
1103	24-25	0.19836746855214926
1103	26-27	0.22664293007673564
1103	28-29	0.28648003955181167
1103	30-31	0.15947958533608642
1103	32-33	0.17949014576100808
1103	34-35	0.20166448907409773
1103	36-37	0.24080526885906295
1103	38-39	0.23650762388743374
1103	40-41	0.3237243693112788
1103	42-43	0.3346170255588916
1103	44-45	0.2527835721385685
1103	46-47	0.29945719033551654
1103	48-49	0.4104205259222695
1103	50-51	0.33902233813618565
1103	52-53	0.26256684993490964
1103	54-55	0.31654993831208955
1103	56-57	0.3396293566914821
1103	58-59	0.29408004387022757
1103	60-61	0.2703993824685611
1103	62-63	0.28402350309419333
1103	64-65	0.42667728069527655
1103	66-67	0.4374289347511642
1103	68-69	0.37394293087876207
1103	70-71	0.41320248629540757
1103	72-73	0.40602173400812447
1103	74-75	0.3567701645429615
1103	76-77	0.402871619423923
1103	78-79	0.45692612890739426
1103	80-81	0.4742169769843976
1103	82-83	0.3499314052899791
1103	84-85	0.21991009297920172
1103	86-87	0.24439617037645434
1103	88-89	0.35145352022586707
1103	90-91	0.34258456047736985
1103	92-93	0.4393548642284344
1103	94-95	0.6394629826421863
1103	96-97	0.7184131153421713
1103	98-99	0.7063867820042855
1103	100	0.6696645115773077
1104	1	0.05820290283201501
1104	2	0.06320130715010208
1104	3	0.02648825272799371
1104	4	-0.011356433730540516
1104	5	-0.02933488121304606
1104	6	0.035754037986087894
1104	7	0.004938171557363091
1104	8	0.06225677781146999
1104	9	0.07541602014380544
1104	10-11	0.09866489843646065
1104	12-13	0.07223976078639538
1104	14-15	0.040058011603747445
1104	16-17	0.14991390964917173
1104	18-19	0.1737105273225481
1104	20-21	0.1843972066830375
1104	22-23	0.24150720683187643
1104	24-25	0.17795871623359005
1104	26-27	0.1776066928602873
1104	28-29	0.15638884043709567
1104	30-31	0.15692105961863945
1104	32-33	0.2091233329476836
1104	34-35	0.27163930800887925
1104	36-37	0.2028930498351471
1104	38-39	0.19960963457771186
1104	40-41	0.28245918494409494
1104	42-43	0.24795525095495208
1104	44-45	0.23027993938511315
1104	46-47	0.2135377202946671
1104	48-49	0.29272984164926186
1104	50-51	0.23844654005356603
1104	52-53	0.11079163083204335
1104	54-55	0.23175210132717794
1104	56-57	0.26162997355704576
1104	58-59	0.27735476677490567
1104	60-61	0.2791600474788112
1104	62-63	0.1393869401715726
1104	64-65	0.3216570492917086
1104	66-67	0.36533827367231453
1104	68-69	0.30172506133759214
1104	70-71	0.3896213268169788
1104	72-73	0.3828657753802176
1104	74-75	0.3252557305370267
1104	76-77	0.3682334784754069
1104	78-79	0.4379391893596605
1104	80-81	0.3747045836908036
1104	82-83	0.28514471477437553
1104	84-85	0.2707095982100043
1104	86-87	0.33595965317513077
1104	88-89	0.4154429050987112
1104	90-91	0.44116710764630795
1104	92-93	0.48355664322197356
1104	94-95	0.551030803329823
1104	96-97	0.5922903440406255
1104	98-99	0.6298831203101223
1104	100	0.5878080482024064
1105	1	-0.09462982029787526
1105	2	-0.09507997782110422
1105	3	-0.09450560879150416
1105	4	-0.10145643547500072
1105	5	-0.11853440094253642
1105	6	-0.2060766379522505
1105	7	-0.1037201876828604
1105	8	-0.13340641316072777
1105	9	-0.09033189280382459
1105	10-11	-0.07849751902627133
1105	12-13	-0.23336969227452897
1105	14-15	-0.23545653893113183
1105	16-17	-0.1321832352336827
1105	18-19	-0.2788168025539335
1105	20-21	-0.1261511412610119
1105	22-23	-0.17008598942164355
1105	24-25	-0.23038989898290652
1105	26-27	-0.10355108610927743
1105	28-29	-0.18012388857132322
1105	30-31	-0.08867854631240846
1105	32-33	-0.14749934813249155
1105	34-35	-0.06769667972211124
1105	36-37	-0.06490585447254205
1105	38-39	-0.1451691771939565
1105	40-41	-0.15596181837709366
1105	42-43	-0.04463475680631035
1105	44-45	-0.07710912766373212
1105	46-47	-0.10350954228037068
1105	48-49	-0.10350383318911227
1105	50-51	-0.09503667652688463
1105	52-53	-0.11413215726073389
1105	54-55	-0.004534494393439559
1105	56-57	0.006323813495178854
1105	58-59	-0.012741838375326608
1105	60-61	0.032341866505298356
1105	62-63	-0.019913128942253167
1105	64-65	0.05627746780238141
1105	66-67	0.14722430468665593
1105	68-69	0.04133923763389902
1105	70-71	0.11653066733521555
1105	72-73	0.1976982143081898
1105	74-75	0.17598122363623503
1105	76-77	0.270105899832668
1105	78-79	0.23458529833032316
1105	80-81	0.0655316537622781
1105	82-83	0.28306377104286184
1105	84-85	0.2566562888881414
1105	86-87	0.38201729452758215
1105	88-89	0.4054937010534694
1105	90-91	0.3551642445371357
1105	92-93	0.4032223603794485
1105	94-95	0.421358499823306
1105	96-97	0.5243962231393429
1105	98-99	0.6339529873508791
1105	100	0.6665273002075409
1106	1	-0.1940685707816563
1106	2	-0.15675227365462518
1106	3	-0.18223014401029047
1106	4	-0.1567757480898635
1106	5	-0.16485138510856956
1106	6	-0.14886370355474554
1106	7	-0.1369063317661059
1106	8	-0.1705857956076784
1106	9	-0.187549795386019
1106	10-11	-0.16996750284079098
1106	12-13	-0.2247488488771694
1106	14-15	-0.20821717264412598
1106	16-17	-0.20099189088512048
1106	18-19	-0.23942461176361007
1106	20-21	-0.24488447000861413
1106	22-23	-0.2580697491259514
1106	24-25	-0.26454479294903877
1106	26-27	-0.3297476480923791
1106	28-29	-0.2655034373761751
1106	30-31	-0.3396445400495196
1106	32-33	-0.35548467022850616
1106	34-35	-0.2712809413063937
1106	36-37	-0.2558441113393286
1106	38-39	-0.320664437507439
1106	40-41	-0.20805239593724423
1106	42-43	-0.2798084445862159
1106	44-45	-0.19849056697349
1106	46-47	-0.28362427033363247
1106	48-49	-0.22897536299848298
1106	50-51	-0.27242231557589847
1106	52-53	-0.2346987561947138
1106	54-55	-0.1660223152584983
1106	56-57	-0.31040100473197896
1106	58-59	-0.24138569860029335
1106	60-61	-0.2906803233463684
1106	62-63	-0.18689642481228574
1106	64-65	-0.15585811051404264
1106	66-67	-0.15205433778742616
1106	68-69	-0.2195526958393863
1106	70-71	-0.15590884493517976
1106	72-73	-0.18397183194171873
1106	74-75	-0.1764515832850755
1106	76-77	-0.06773722180180997
1106	78-79	-0.12172559514801407
1106	80-81	-0.21904035861307491
1106	82-83	-0.12737575696759507
1106	84-85	-0.23655275660222586
1106	86-87	-0.12934895120064738
1106	88-89	-0.21603849321149582
1106	90-91	-0.3290229858606253
1106	92-93	-0.2723807464439041
1106	94-95	-0.19099268493682686
1106	96-97	-0.02773086135770697
1106	98-99	0.10434817555406006
1106	100	0.03162215933196322
1107	1	-0.37643062951930517
1107	2	-0.32178683604374925
1107	3	-0.27981551980066044
1107	4	-0.29451369637477853
1107	5	-0.29054018010191385
1107	6	-0.2458623209866957
1107	7	-0.29045433377064
1107	8	-0.3033215517311163
1107	9	-0.3141353356585128
1107	10-11	-0.3265384879112432
1107	12-13	-0.34882609574025736
1107	14-15	-0.4338265343899934
1107	16-17	-0.49442273023622363
1107	18-19	-0.4402386140537473
1107	20-21	-0.520522727604714
1107	22-23	-0.44913745979832953
1107	24-25	-0.4582104873468751
1107	26-27	-0.3546539342739763
1107	28-29	-0.3532775053365995
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2207	96-97	0.5881777340947121
2207	98-99	0.5790803096376216
2207	100	0.4323505027915022
2208	1	-0.2976955242145678
2208	2	-0.2850755833628362
2208	3	-0.2449177126227262
2208	4	-0.26287861918641653
2208	5	-0.3079285549626576
2208	6	-0.30144490881441044
2208	7	-0.25601746199113506
2208	8	-0.29893299238683113
2208	9	-0.30000501012288083
2208	10-11	-0.24139963816284649
2208	12-13	-0.328507091354183
2208	14-15	-0.574702096715825
2208	16-17	-0.4651243757203076
2208	18-19	-0.5511753555691428
2208	20-21	-0.529239007569771
2208	22-23	-0.5771225932207713
2208	24-25	-0.5729775698917194
2208	26-27	-0.7477041906341242
2208	28-29	-0.6092995194173554
2208	30-31	-0.6530507573006261
2208	32-33	-0.5016812711246672
2208	34-35	-0.5593033242345911
2208	36-37	-0.6164130920250557
2208	38-39	-0.5620364310787735
2208	40-41	-0.8487451233903158
2208	42-43	-0.41349722887566287
2208	44-45	-0.3056169223910672
2208	46-47	-0.15450141459955802
2208	48-49	-0.417091742868557
2208	50-51	-0.7010046334890916
2208	52-53	-0.17923142613672738
2208	54-55	-0.023283655387867697
2208	56-57	0.01738092859218554
2208	58-59	-0.5799720571900338
2208	60-61	-0.23141146298246795
2208	62-63	-0.3912102812780631
2208	64-65	-0.15338260614225163
2208	66-67	-0.3242714760540366
2208	68-69	-0.1436291143408681
2208	70-71	-0.18338028826531882
2208	72-73	-0.20703626042737255
2208	74-75	-0.6501523614237819
2208	76-77	-0.48122194054532486
2208	78-79	-0.9364521420934153
2208	80-81	-0.7945651554052553
2208	82-83	-0.5606959148762272
2208	84-85	-0.8332411568297395
2208	86-87	-1.0958028304657574
2208	88-89	-1.3347429921747924
2208	90-91	-1.2100145197268368
2208	92-93	-1.6445091108192464
2208	94-95	-2.012279485281642
2208	96-97	-1.6456086089099404
2208	98-99	-1.8879268068001753
2208	100	-1.9946755291566731
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	408022.0
3	170246.0
4	154532.0
5	124844.0
6	113875.0
7	118331.0
8	124475.0
9	138872.0
10	157634.0
11	177648.0
12	195377.0
13	227975.0
14	278386.0
15	351522.0
16	415590.0
17	419742.0
18	387785.0
19	365359.0
20	366800.0
21	381176.0
22	398457.0
23	421477.0
24	440828.0
25	467500.0
26	500730.0
27	544325.0
28	605735.0
29	685998.0
30	784942.0
31	908446.0
32	1074443.0
33	1314351.0
34	1687424.0
35	2274969.0
36	2833273.0
37	2342463.0
38	704369.0
39	25693.0
40	16.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.71306102084644	28.035154775591664	27.983092908739888	22.268691294822013
2	23.61562941725214	28.111459199967992	27.23338584905705	21.039525533722816
3	23.33515161994441	26.506561312568234	27.06745877969368	23.090828287793677
4	24.407494337890448	25.978105066234775	26.67465929906765	22.939741296807135
5	24.621522088723903	26.29311102766564	26.424784517948645	22.660582365661813
6	24.636903840428698	26.150259005656444	25.90411648488457	23.308720669030286
7	24.641898099869945	26.48595117712721	26.034360217486352	22.837790505516494
8	24.017403025872454	26.299113067223328	26.410494476899952	23.272989430004262
9	23.9204915206333	25.981049479220573	26.54858111892976	23.549877881216368
10-11	23.901607875859675	26.14553051770908	26.452758261820293	23.500103344610952
12-13	23.617268690479744	26.202941407188042	26.642881316926204	23.53690858540601
14-15	23.232425373972177	26.498353375024173	27.16727129131425	23.101949959689406
16-17	23.057562561393073	26.380615577121308	27.393656500871522	23.1681653606141
18-19	22.59289449727191	26.362344584746882	28.187661735379947	22.85709918260126
20-21	22.266637290816714	26.05778721111949	29.108642758206944	22.566932739856853
22-23	22.320969624359517	25.99474095612184	29.46911651016442	22.215172909354227
24-25	22.19253769152865	25.843195073120484	29.966528682680188	21.99773855267068
26-27	22.24610358187918	25.908735532886666	29.966877874883757	21.878283010350394
28-29	22.317170094076964	25.941746120715187	29.943914356255057	21.797169428952788
30-31	22.301033859687543	25.86572274880644	29.911424659056525	21.921818732449488
32-33	22.284966911756822	25.975207956067436	29.79892883101763	21.940896301158112
34-35	22.386825683405917	25.966586113763945	29.847227940376907	21.79936026245323
36-37	22.36796423073681	25.97180358404106	29.73738096422236	21.92285122099977
38-39	22.414507807696122	26.01753421840988	29.600991507915115	21.966966465978885
40-41	22.471291729483895	26.177323387201334	29.376056583096766	21.975328300218006
42-43	22.67172316708157	26.24155007505788	28.873528604994757	22.21319815286579
44-45	22.717082572288323	26.50091918662258	28.419376184654727	22.362622056434372
46-47	22.99570398150199	26.656267442811394	27.94581999030548	22.402208585381132
48-49	23.10022403757656	26.659077127152504	27.552388664107873	22.68831017116306
50-51	23.141317841303902	26.731426429481775	27.359966624628445	22.767289104585878
52-53	23.322333948394615	26.79034933246232	27.13950120011007	22.74781551903299
54-55	23.367143159546035	26.732433666550953	27.08151662519635	22.81890654870666
56-57	23.361691765046867	26.819647991517915	27.02242226471956	22.796237978715663
58-59	23.472479093924445	26.879205046754407	26.930808065115247	22.7175077942059
60-61	23.3963521739442	26.879208429025148	26.860583749254562	22.863855647776084
62-63	23.495859310259775	26.904547093817644	26.80111429408814	22.798479301834448
64-65	23.51104383783011	27.009093498745578	26.718339804438635	22.761522858985682
66-67	23.570404318003874	27.018657781330457	26.572329689260282	22.838608211405386
68-69	23.55557261966881	27.101989086590066	26.492644327750163	22.849793965990965
70-71	23.63889644613623	27.23605956375497	26.368433434163034	22.756610555945763
72-73	23.6350996646481	27.205116242239885	26.250074512660138	22.909709580451878
74-75	23.69443491170212	27.34951675762992	26.141958112534773	22.814090218133178
76-77	23.257539293619402	27.728579908190042	25.94156547666981	23.072315321520747
78-79	23.423184536149343	27.698461365874145	25.826794538045387	23.05155955993113
80-81	23.407777391944826	27.768078543165025	25.726215049545058	23.09792901534509
82-83	23.403967347867425	27.88959640088729	25.597648127717903	23.108788123527383
84-85	23.341210963721327	27.897967580021394	25.486836691828312	23.27398476442897
86-87	23.272562308315084	28.05874821111401	25.443343629279425	23.22534585129148
88-89	23.357664849984253	28.131668131584114	25.300186097061495	23.210480921370138
90-91	23.209754532133385	28.133769474277077	25.187182809129077	23.469293184460465
92-93	23.220272317989632	28.230959788600224	25.08751055355516	23.46125733985498
94-95	23.230472732140033	28.308532480965816	24.89742552626287	23.563569260631283
96-97	23.144436615636362	28.416412418058695	24.693657195252385	23.745493771052555
98-99	23.118033615503645	28.429721265804847	24.604236781245632	23.848008337445876
100	23.880912679685558	28.360297552014487	23.922843675893795	23.835946092406164
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	518.0
1	362.5
2	184.5
3	161.5
4	166.5
5	206.0
6	256.0
7	332.0
8	447.0
9	591.5
10	792.5
11	1051.5
12	1471.0
13	2167.0
14	3597.0
15	5405.5
16	8028.5
17	10602.5
18	12705.0
19	16692.0
20	26501.5
21	33511.5
22	38001.0
23	47447.5
24	58411.0
25	71015.5
26	85644.5
27	104322.5
28	125777.0
29	146977.0
30	169326.0
31	197106.0
32	238000.5
33	288290.5
34	339410.5
35	400705.0
36	477467.0
37	574771.0
38	668461.5
39	723056.0
40	772407.5
41	850778.0
42	975247.0
43	1087004.5
44	1148168.0
45	1178314.5
46	1162323.0
47	1110918.0
48	1048104.5
49	1008327.0
50	962334.0
51	884840.5
52	793188.5
53	687675.0
54	600092.0
55	530404.5
56	458573.0
57	398938.0
58	353025.5
59	310688.0
60	268562.5
61	232221.5
62	202719.5
63	177590.5
64	155042.0
65	134911.5
66	116766.5
67	100380.0
68	85549.5
69	72171.5
70	60484.5
71	50882.0
72	42821.0
73	36170.5
74	30305.5
75	24753.0
76	20278.5
77	16796.5
78	14064.5
79	11658.0
80	9533.5
81	7674.5
82	6020.5
83	4607.5
84	3346.5
85	2359.0
86	1598.5
87	1072.5
88	756.5
89	513.5
90	355.5
91	239.0
92	148.0
93	101.5
94	60.5
95	37.0
96	25.0
97	11.0
98	6.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19109165601077005
2	9.916154368109303E-4
3	0.001996221468863925
4	0.006625203573452939
5	0.020351932545901185
6	0.002186750199080872
7	0.009344568177458459
8	0.011704526313100191
9	0.016524037147906154
10-11	0.004282566231467292
12-13	0.0030462945842641455
14-15	0.009987602641940657
16-17	0.0027453457944896496
18-19	0.00320001662796191
20-21	0.01891863687086006
22-23	0.013514549250161193
24-25	0.011542143872574386
26-27	0.0013207105162765663
28-29	8.573792859762627E-4
30-31	0.012033621392565828
32-33	0.012451485539518905
34-35	0.008543481470864476
36-37	0.003163209941442727
38-39	0.004877968513395252
40-41	0.006512618414688379
42-43	0.0019550845839307203
44-45	0.004031414723454043
46-47	0.005202733394446867
48-49	0.011420898316981782
50-51	0.004992718771366823
52-53	0.014064484448741924
54-55	0.010453098971447972
56-57	0.00521139379127491
58-59	0.008476363395447143
60-61	0.002665237123830251
62-63	0.005811126271616891
64-65	0.007569186827709633
66-67	0.005198403196032845
68-69	0.01835138087862324
70-71	0.01390859730583715
72-73	0.008298825260472261
74-75	0.009368384268735578
76-77	0.00920816692741678
78-79	0.026446686813636485
80-81	0.009416016451289814
82-83	0.004544543235515595
84-85	0.01268748135308308
86-87	0.004529387541066519
88-89	0.010626306908008833
90-91	0.014008191869359645
92-93	0.0030354690882290914
94-95	0.0033537386716596743
96-97	0.006783255815564725
98-99	0.007214110557759866
100	0.004018424128211979
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.309363E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.38486109255829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.28074763102678	29.638997375001065
2	11.593999248153928	8.668801027989199
3	3.6789922477598123	4.126158424292982
4	1.5963079231456587	2.387109998710027
5	0.8365556718444572	1.5637258794048405
6	0.4960440270998444	1.1126722229352544
7	0.3368024035932982	0.8813917751782647
8	0.2539557088004889	0.7595279117734769
9	0.18907887114019145	0.6361818599802408
>10	1.3491341951145905	8.95922140495776
>50	0.09981692653927582	2.7079963736754182
>100	0.22498717461960277	20.05690250047189
>500	0.05550630636513279	13.991745316384888
>1k	0.007885261539916462	3.663311295485524
>5k	5.8251017866057356E-5	0.13825385653837985
>10k+	1.2815223930532233E-4	0.7080027772204537
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29563	0.12801365571371845	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.330198414021529E-6	4.330198414021529E-6	0.0	0.0	0.0
2	4.330198414021529E-6	8.660396828043058E-6	0.0	0.0	0.0
3	4.330198414021529E-6	1.2990595242064586E-5	0.0	0.0	0.0
4	4.330198414021529E-6	1.2990595242064586E-5	0.0	0.0	0.0
5	8.660396828043058E-6	1.7320793656086116E-5	0.0	0.0	0.0
6	8.660396828043058E-6	2.5981190484129173E-5	0.0	0.0	0.0
7	8.660396828043058E-6	2.5981190484129173E-5	0.0	0.0	0.0
8	1.2990595242064586E-5	2.5981190484129173E-5	0.0	0.0	0.0
9	3.464158731217223E-5	2.5981190484129173E-5	0.0	0.0	0.0
10-11	8.227376986640904E-5	2.5981190484129173E-5	0.0	4.330198414021529E-6	0.0
12-13	3.117742858095501E-4	3.03113888981507E-5	0.0	4.330198414021529E-6	0.0
14-15	7.123176391065414E-4	3.897178572619376E-5	0.0	4.330198414021529E-6	0.0
16-17	0.001461441964732266	4.763218255423682E-5	0.0	4.330198414021529E-6	0.0
18-19	0.002539661369823627	5.6292579382279874E-5	0.0	4.330198414021529E-6	0.0
20-21	0.003970791945657742	5.8457678589290634E-5	0.0	4.330198414021529E-6	0.0
22-23	0.005761328989855644	6.06227777963014E-5	0.0	4.330198414021529E-6	2.1650992070107645E-6
24-25	0.007841989327792988	6.06227777963014E-5	0.0	4.330198414021529E-6	4.330198414021529E-6
26-27	0.010368660102374552	6.495297621032293E-5	0.0	4.330198414021529E-6	4.330198414021529E-6
28-29	0.012869349686471984	7.577847224537676E-5	0.0	4.330198414021529E-6	4.330198414021529E-6
30-31	0.015192501135594534	7.794357145238752E-5	0.0	4.330198414021529E-6	8.660396828043058E-6
32-33	0.017418223120401602	7.794357145238752E-5	0.0	4.330198414021529E-6	8.660396828043058E-6
34-35	0.01962445921234557	7.794357145238752E-5	0.0	4.330198414021529E-6	1.0825496035053822E-5
36-37	0.02186750199080872	7.794357145238752E-5	0.0	4.330198414021529E-6	1.5155694449075352E-5
38-39	0.02379227518584129	7.794357145238752E-5	0.0	4.330198414021529E-6	1.948589286309688E-5
40-41	0.025992015980164228	8.227376986640904E-5	0.0	6.495297621032294E-6	2.1650992070107644E-5
42-43	0.028328158024528843	9.526436510847363E-5	0.0	8.660396828043058E-6	2.5981190484129173E-5
44-45	0.03065130947365139	1.0175966272950593E-4	2.1650992070107645E-6	8.660396828043058E-6	3.03113888981507E-5
46-47	0.03322344733158018	1.0825496035053823E-4	6.495297621032294E-6	8.660396828043058E-6	3.464158731217223E-5
48-49	0.03616148695549379	1.0825496035053823E-4	8.660396828043058E-6	8.660396828043058E-6	3.464158731217223E-5
50-51	0.03951306052794645	1.0825496035053823E-4	8.660396828043058E-6	8.660396828043058E-6	3.464158731217223E-5
52-53	0.04342755989422191	1.1258515876455975E-4	8.660396828043058E-6	8.660396828043058E-6	3.464158731217223E-5
54-55	0.04819943854647364	1.1258515876455975E-4	8.660396828043058E-6	1.7320793656086116E-5	3.464158731217223E-5
56-57	0.053664148944968806	1.1691535717858128E-4	8.660396828043058E-6	1.7320793656086116E-5	3.464158731217223E-5
58-59	0.06043224906608446	1.1691535717858128E-4	8.660396828043058E-6	1.7320793656086116E-5	3.464158731217223E-5
60-61	0.06857735228285895	1.1691535717858128E-4	8.660396828043058E-6	2.1650992070107644E-5	3.464158731217223E-5
62-63	0.07823585984533396	1.1908045638559204E-4	8.660396828043058E-6	2.1650992070107644E-5	3.464158731217223E-5
64-65	0.08991657006715705	1.212455555926028E-4	8.660396828043058E-6	2.1650992070107644E-5	3.464158731217223E-5
66-67	0.10389661564682556	1.212455555926028E-4	8.660396828043058E-6	2.1650992070107644E-5	3.464158731217223E-5
68-69	0.12066530900512393	1.2990595242064586E-4	8.660396828043058E-6	2.1650992070107644E-5	3.464158731217223E-5
70-71	0.14071196256283658	1.3640125004167817E-4	8.660396828043058E-6	2.1650992070107644E-5	3.897178572619376E-5
72-73	0.16505633804646563	1.3856634924868893E-4	8.660396828043058E-6	2.1650992070107644E-5	3.897178572619376E-5
74-75	0.1935273926186572	1.3856634924868893E-4	8.660396828043058E-6	2.1650992070107644E-5	3.897178572619376E-5
76-77	0.2278052432640516	1.3856634924868893E-4	8.660396828043058E-6	2.1650992070107644E-5	3.897178572619376E-5
78-79	0.2676538941690847	1.3856634924868893E-4	8.660396828043058E-6	2.5981190484129173E-5	4.330198414021529E-5
80-81	0.3145889147786641	1.407314484556997E-4	8.660396828043058E-6	2.8146289691139937E-5	4.330198414021529E-5
82-83	0.3686427815808948	1.450616468697212E-4	8.660396828043058E-6	3.03113888981507E-5	4.330198414021529E-5
84-85	0.4315843806279047	1.4722674607673197E-4	8.660396828043058E-6	3.2476488105161465E-5	4.330198414021529E-5
86-87	0.5031603953124736	1.4722674607673197E-4	8.660396828043058E-6	3.6806686519183E-5	4.5467083347226056E-5
88	0.562371528425804	1.4722674607673197E-4	1.2990595242064586E-5	3.897178572619376E-5	4.763218255423682E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	16025	0.0	47.397327	1
TCTCGAG	19275	0.0	39.279564	2
CTCGAGG	24580	0.0	30.880836	3
GATCCGG	25265	0.0	30.566242	1
TCGAGGG	30260	0.0	25.006481	4
TATCTCG	8820	0.0	24.990202	2
ATCCGGC	31145	0.0	24.701643	2
TCCGGCG	41540	0.0	18.736664	3
ATATCTC	12130	0.0	18.43955	1
GGATCCG	16465	0.0	18.239151	2
GCGCCGT	43315	0.0	17.771734	9
CGAGGGC	44350	0.0	17.188753	5
CGGCGCG	45115	0.0	16.991047	5
ATGGATC	16895	0.0	16.834345	1
AGGGCGC	48030	0.0	15.636179	7
CGTCGAC	41425	0.0	15.552998	12-13
CCGGCGC	50050	0.0	15.456862	4
TGGATCC	20175	0.0	15.451191	1
TCGACTT	43100	0.0	14.994235	14-15
GCCGTCG	44360	0.0	14.709446	10-11
>>END_MODULE