FastQCFastQC Report
Wed 25 May 2016
SRR491022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28529812
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA175200.048.5878641
TCTCGAG211500.039.9976652
CTCGAGG266900.031.486653
GATCCGG287150.029.6451111
TCGAGGG319450.026.2333954
ATCCGGC344650.024.7088432
TATCTCG89750.020.5118941
TCCGGCG436200.019.5137313
CGAGGGC462850.018.227155
GCGCCGT469350.017.9227399
GGATCCG185300.017.197363
AGGGCGC495200.016.8455377
CGGCGCG507600.016.6109855
ATGGATC211750.015.8314281
CCGGCGC560000.015.24171454
CGTCGAC451550.015.09062712-13
GGGCGCG565700.014.7461748
GAGGGCG580000.014.5122766
GCCGTCG473800.014.49108810-11
TCGACTT473100.014.42002214-15
Produced by FastQC (version 0.11.5)