FastQCFastQC Report
Wed 25 May 2016
SRR491021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29603623
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1272350.42979536660090556No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1004280.33924226098947413No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950080.32093369112287373No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT886910.29959508672300006No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT880400.297396031560056No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT591760.19989445210810852No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550540.18597048070771607No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT507660.1714857671305975No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409110.13819592284363302No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382630.1292510717353751No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364410.12309641965106771No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298930.10097750535466554No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296150.10003843110689527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC317850.063.430461
TATCTCG344450.056.4887242
ATGGATC392200.044.185783
TATGGAT418200.041.8292352
ATCTCGA492500.039.0141073
TGGATCC461950.037.3101544
GGATCCG441100.037.250895
ATATGGA481750.036.9146461
TCTCGAG556850.033.821454
GATCCGG644800.028.5804621
CTCGAGG663300.028.2513075
TCGAGGG748500.024.933586
ATCCCGC56000.023.8345476
ATCCGGC780950.023.434752
TCCGGCG966400.018.9438173
CGAGGGC982900.018.901317
GATCCCG84600.018.3890765
TCCCGCG70000.017.2547847
AGGGCGC1118000.016.5536469
GGATCCC101000.016.3806954
Produced by FastQC (version 0.11.5)