FastQCFastQC Report
Wed 25 May 2016
SRR491020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22083970
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA909920.41202736645630295No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT851410.38553303595322763No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT688170.3116151670193358No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530400.2401742078077447No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT436810.197795052248305No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349590.15830034183165437No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321000.14535429997414415No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270690.12257306996885071No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265400.12017766733064754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG216200.060.742532
ATATCTC237500.056.2357371
ATCTCGA307250.042.417573
ATGGATC272250.040.9136473
TATGGAT277850.040.4643862
ATATGGA288050.039.3327181
TCTCGAG347450.037.1446234
GGATCCG293000.036.9577565
TGGATCC308300.036.0380864
CTCGAGG416600.030.9906085
GATCCGG448250.029.3763331
TCGAGGG468550.027.4640926
ATCCGGC551850.023.7036022
CGAGGGC606450.021.0718177
TCCGGCG679800.019.2959613
AGGGCGC731650.017.2474849
ATCCCGC39950.016.587856
GAGGGCG784100.016.2616318
GCGCCGT803750.016.0803879
CGGCGCG813650.015.9542635