##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491020_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22083970
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07303899615875	31.0	31.0	34.0	28.0	34.0
2	31.66961583447179	33.0	31.0	34.0	28.0	34.0
3	31.890409151977657	34.0	31.0	34.0	30.0	34.0
4	35.37036655999805	37.0	35.0	37.0	33.0	37.0
5	35.188231282690566	37.0	35.0	37.0	32.0	37.0
6	35.12952938262459	37.0	35.0	37.0	32.0	37.0
7	35.08544550640125	37.0	35.0	37.0	32.0	37.0
8	34.98551256861877	37.0	35.0	37.0	32.0	37.0
9	36.52066802300492	39.0	37.0	39.0	32.0	39.0
10-11	36.35845128842323	39.0	37.0	39.0	32.0	39.0
12-13	36.14091687318901	39.0	37.0	39.0	32.0	39.0
14-15	37.399208611495126	40.0	38.0	41.0	32.0	41.0
16-17	37.34548237930046	40.0	38.0	41.0	32.0	41.0
18-19	37.2027322985858	40.0	38.0	41.0	31.5	41.0
20-21	37.09175422263298	40.0	38.0	41.0	31.0	41.0
22-23	36.939154101368544	40.0	37.5	41.0	31.0	41.0
24-25	36.658986246585194	40.0	37.0	41.0	30.0	41.0
26-27	36.21870707576581	39.5	36.0	41.0	30.0	41.0
28-29	35.86164758872612	39.0	35.0	41.0	28.5	41.0
30-31	35.53746504817748	39.0	35.0	40.0	27.5	41.0
32-33	35.238940711294205	39.0	35.0	40.0	25.5	41.0
34-35	34.861308383411135	39.0	35.0	40.0	24.5	41.0
36-37	34.4863354052736	38.0	34.5	40.0	23.0	41.0
38-39	34.05852410141836	38.0	34.0	40.0	19.0	41.0
40-41	33.54577496709152	38.0	33.0	40.0	12.0	41.0
42-43	32.946231452044174	38.0	33.0	40.0	5.0	41.0
44-45	32.35929078874858	38.0	32.5	40.0	2.0	41.0
46-47	31.863248636907223	37.5	31.5	40.0	2.0	41.0
48-49	31.302405455178572	37.0	30.5	40.0	2.0	41.0
50-51	30.676662461504883	36.5	29.5	39.0	2.0	40.0
52-53	30.14773525774578	36.0	28.5	39.0	2.0	40.0
54-55	29.504400544829576	35.0	27.0	39.0	2.0	40.0
56-57	28.973209006351667	35.0	26.0	39.0	2.0	40.0
58-59	28.399311944365074	34.5	25.0	38.0	2.0	40.0
60-61	27.77072390063924	34.0	23.0	38.0	2.0	40.0
62-63	27.153499551937443	34.0	21.0	38.0	2.0	40.0
64-65	26.490555638320465	33.0	18.5	37.0	2.0	39.0
66-67	25.723610451381703	32.5	12.0	36.0	2.0	39.0
68-69	24.972633090879945	31.5	7.5	35.5	2.0	38.5
70-71	24.228588428620398	31.0	2.0	35.0	2.0	38.0
72-73	23.42982312509934	30.5	2.0	35.0	2.0	37.0
74-75	22.565217349960175	29.5	2.0	34.0	2.0	36.5
76-77	21.61036971613347	28.5	2.0	33.5	2.0	35.0
78-79	21.600183572066072	29.0	2.0	34.0	2.0	35.0
80-81	20.95540448569709	29.0	2.0	34.0	2.0	35.0
82-83	20.193891157251166	27.0	2.0	33.0	2.0	35.0
84-85	19.42544286647736	26.0	2.0	33.0	2.0	35.0
86-87	18.656391128950094	25.0	2.0	32.0	2.0	34.0
88-89	17.84625579096512	23.5	2.0	32.0	2.0	34.0
90-91	16.702265829015346	19.0	2.0	31.0	2.0	34.0
92-93	15.871407858279106	10.0	2.0	31.0	2.0	34.0
94-95	14.842080771709071	2.0	2.0	31.0	2.0	34.0
96-97	13.666688484905567	2.0	2.0	29.5	2.0	34.0
98-99	12.699875384724757	2.0	2.0	29.0	2.0	34.0
100	11.944040949159051	2.0	2.0	27.0	2.0	33.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.32495140320006755
1101	2	0.27062786562125396
1101	3	0.2608092670290283
1101	4	0.22428282982208714
1101	5	0.24609512340971662
1101	6	0.2484015148776919
1101	7	0.2843588055435262
1101	8	0.28975662885095943
1101	9	0.3284683573219027
1101	10-11	0.26508180603966736
1101	12-13	0.25171882728519535
1101	14-15	0.27069937390845666
1101	16-17	0.27024068535848755
1101	18-19	0.27106024803890705
1101	20-21	0.2842386137347148
1101	22-23	0.20556194956225227
1101	24-25	0.19420919793420666
1101	26-27	0.23648798931180437
1101	28-29	0.1891616475233917
1101	30-31	0.21641752576665851
1101	32-33	0.1559760925310485
1101	34-35	0.18700866925833992
1101	36-37	0.03179231942261396
1101	38-39	0.05694609777936677
1101	40-41	-0.027943009277883846
1101	42-43	0.013298583012073095
1101	44-45	-0.015598002152351853
1101	46-47	-0.07026450829726016
1101	48-49	-0.11811444983052866
1101	50-51	-0.12244467710365114
1101	52-53	-0.10042461252193036
1101	54-55	-0.16424200586526894
1101	56-57	-0.3245098348222015
1101	58-59	-0.3160060571139205
1101	60-61	-0.25651374335917865
1101	62-63	-0.44757477028833037
1101	64-65	-0.48512561792317754
1101	66-67	-0.35291609809130975
1101	68-69	-0.5314429203166888
1101	70-71	-0.4599476297083065
1101	72-73	-0.559409152753993
1101	74-75	-0.5436441552043014
1101	76-77	-0.6726721614961697
1101	78-79	-0.631602109252384
1101	80-81	-0.1790882361031052
1101	82-83	-0.3642299342950892
1101	84-85	-0.32503334164110953
1101	86-87	-0.2641747872197229
1101	88-89	-0.34381082420334863
1101	90-91	-0.16996914367718574
1101	92-93	-0.07937085957837553
1101	94-95	-0.1690647892490027
1101	96-97	-0.40567528096337746
1101	98-99	-0.6308514963735679
1101	100	-0.439252535435827
1102	1	0.21961467193213124
1102	2	0.1706867492858848
1102	3	0.21194448767059626
1102	4	0.1845273113128485
1102	5	0.18465468010741404
1102	6	0.2039557713665019
1102	7	0.22808808531160452
1102	8	0.1933183684279811
1102	9	0.21496174283895897
1102	10-11	0.17120108534349754
1102	12-13	0.1976926211062775
1102	14-15	0.25733519604884236
1102	16-17	0.2442162305843354
1102	18-19	0.23525806485482548
1102	20-21	0.21854328157854752
1102	22-23	0.22417574226899717
1102	24-25	0.22578614444287126
1102	26-27	0.1839587187790741
1102	28-29	0.17090941496013556
1102	30-31	0.16117676284756044
1102	32-33	0.1348388361696209
1102	34-35	0.09721139124649625
1102	36-37	0.08534546710578184
1102	38-39	0.07757044333584417
1102	40-41	0.06809201679683241
1102	42-43	-0.010531254459770878
1102	44-45	0.03622540972570931
1102	46-47	-0.09839206490326902
1102	48-49	-0.11259442179056123
1102	50-51	-0.09272673071849624
1102	52-53	-0.07372319021609286
1102	54-55	-0.006249642357087026
1102	56-57	-0.2380454994299086
1102	58-59	-0.30327216982164984
1102	60-61	-0.2313234534066524
1102	62-63	-0.3736120098005351
1102	64-65	-0.35297977210261067
1102	66-67	-0.2778004197309407
1102	68-69	-0.44444005164274714
1102	70-71	-0.2972637807777012
1102	72-73	-0.3273360676453194
1102	74-75	-0.37577133984548183
1102	76-77	-0.3642199291879926
1102	78-79	-0.43176664546891175
1102	80-81	-0.03817449966146569
1102	82-83	-0.19932314918003158
1102	84-85	-0.19506617733360443
1102	86-87	-0.0883968431967368
1102	88-89	-0.17930862826434435
1102	90-91	-0.10151859695365673
1102	92-93	0.03420712435075046
1102	94-95	-0.02997551061910997
1102	96-97	-0.23339098068630193
1102	98-99	-0.39967768814957516
1102	100	-0.20612916065983633
1103	1	0.11968523677906262
1103	2	0.08962190894929734
1103	3	0.07188196265001778
1103	4	0.07448212423909695
1103	5	0.08843198764900251
1103	6	-0.0073208685887422575
1103	7	-0.007123123328973691
1103	8	0.030596437879580662
1103	9	-0.028487862398321795
1103	10-11	-0.02221037881957244
1103	12-13	-0.04655780965321554
1103	14-15	0.03766870619912055
1103	16-17	0.09084014658046158
1103	18-19	0.10645177723529287
1103	20-21	0.06393046653617773
1103	22-23	0.033223478610992174
1103	24-25	0.09625138755075113
1103	26-27	0.03920507044335153
1103	28-29	0.003945656056231428
1103	30-31	-0.060898763705836245
1103	32-33	-0.14769409519652754
1103	34-35	-0.12907667663592548
1103	36-37	-0.2027672081734906
1103	38-39	-0.03764957620491316
1103	40-41	-0.16013100360807897
1103	42-43	-0.20497737795398052
1103	44-45	-0.2579198365660389
1103	46-47	-0.5312524017960492
1103	48-49	-0.4882710080859418
1103	50-51	-0.26213780041340584
1103	52-53	-0.35744422664221176
1103	54-55	-0.29201826438285394
1103	56-57	-0.4261386630160615
1103	58-59	-0.5113353794550157
1103	60-61	-0.36080305297337745
1103	62-63	-0.4708350106356747
1103	64-65	-0.38231386561728087
1103	66-67	-0.571905475188526
1103	68-69	-0.7874129974238997
1103	70-71	-0.5511981719194274
1103	72-73	-0.362835355186526
1103	74-75	-0.3788302891236981
1103	76-77	-0.39097141254271506
1103	78-79	-0.39601865657019175
1103	80-81	0.014840073772209195
1103	82-83	-0.1749307108400373
1103	84-85	-0.07780996870262058
1103	86-87	-0.036667061537180246
1103	88-89	-0.4945546981369162
1103	90-91	-0.29043117370364513
1103	92-93	-0.11054700813767226
1103	94-95	0.009785074602643107
1103	96-97	-0.07182403618639199
1103	98-99	-0.2822236793178501
1103	100	-0.3570065812507668
1104	1	0.05045717110213488
1104	2	0.04013373254581509
1104	3	0.049381288827685665
1104	4	0.041643156752805055
1104	5	0.06485146079945281
1104	6	0.07212584465920457
1104	7	0.09383520980440352
1104	8	0.0614042836619646
1104	9	0.033280153405414126
1104	10-11	0.03547281738530472
1104	12-13	0.026643979272655827
1104	14-15	0.05841313841219886
1104	16-17	0.07504964433751837
1104	18-19	0.0858123363744312
1104	20-21	0.041078764577157756
1104	22-23	0.09341649201114421
1104	24-25	0.10050730955286014
1104	26-27	0.0958385230939598
1104	28-29	0.05173286076500716
1104	30-31	0.05851281278101794
1104	32-33	0.05366794031401412
1104	34-35	0.027090882810121286
1104	36-37	0.03275526316188859
1104	38-39	0.09127548369403371
1104	40-41	-0.019198709972272354
1104	42-43	-0.03558338702909225
1104	44-45	0.048247969408457436
1104	46-47	0.09960663190196684
1104	48-49	0.06228138775969683
1104	50-51	0.07969179114855152
1104	52-53	0.06425264775301187
1104	54-55	0.053079438444679994
1104	56-57	-0.0548330626290543
1104	58-59	-0.017872194508335326
1104	60-61	-0.005524035625811052
1104	62-63	-0.12470880712578136
1104	64-65	-0.10648737631219163
1104	66-67	0.12579033600382772
1104	68-69	0.04531759504509125
1104	70-71	0.007484982707353538
1104	72-73	0.0454418465750166
1104	74-75	0.0030580922691498813
1104	76-77	0.002890915764364621
1104	78-79	0.03313978217798308
1104	80-81	0.43149781766264894
1104	82-83	0.30773832953700264
1104	84-85	0.30556273312324933
1104	86-87	0.4551189625674077
1104	88-89	0.11255814712729872
1104	90-91	0.2774849836079021
1104	92-93	0.4208494003630747
1104	94-95	0.4710385005332576
1104	96-97	0.3634228827984014
1104	98-99	0.34157821877772143
1104	100	0.4110512621323945
1105	1	-0.08345809196899978
1105	2	-0.03279896108005431
1105	3	-0.012168121704249302
1105	4	-0.004535192762368467
1105	5	-0.0069705620266447
1105	6	-0.04468843005253831
1105	7	-0.008095487195369344
1105	8	-0.027837062852839267
1105	9	-0.039078273718793355
1105	10-11	-0.04110466388200251
1105	12-13	-0.07075751917201245
1105	14-15	-0.08207660554585061
1105	16-17	-0.022765846109365384
1105	18-19	0.0015465950009740936
1105	20-21	7.874161358003562E-4
1105	22-23	-0.028604507202203422
1105	24-25	0.015640183588125467
1105	26-27	0.01026140562185418
1105	28-29	0.0197695779521041
1105	30-31	0.025497376340709366
1105	32-33	0.0015134642482550476
1105	34-35	0.04859353999048466
1105	36-37	0.09338228927716585
1105	38-39	0.13846432494631955
1105	40-41	0.08283747362954585
1105	42-43	-0.02281761067436605
1105	44-45	0.06455875174897585
1105	46-47	0.041193893677426985
1105	48-49	0.035052741682420674
1105	50-51	0.06729220601894781
1105	52-53	0.07847672758664359
1105	54-55	0.20048939155873313
1105	56-57	0.06617697745320328
1105	58-59	0.03893430861314329
1105	60-61	-0.01772724509008583
1105	62-63	-0.06529248622612727
1105	64-65	0.0015961461591409432
1105	66-67	0.14064861804846274
1105	68-69	0.06422387173176602
1105	70-71	0.04295976304384297
1105	72-73	0.046825400897635205
1105	74-75	0.15110900330085997
1105	76-77	0.22261018416474698
1105	78-79	0.15718295464393606
1105	80-81	0.5919930581142729
1105	82-83	0.4168940280668494
1105	84-85	0.21664690850497337
1105	86-87	0.3267709683689688
1105	88-89	0.20726630658413825
1105	90-91	0.18203106452653728
1105	92-93	0.5904950503904587
1105	94-95	0.6235918306016863
1105	96-97	0.5196567508897285
1105	98-99	0.45833080704402995
1105	100	0.45417037338326693
1106	1	-0.1895728903084688
1106	2	-0.14793073449843774
1106	3	-0.17057137425828373
1106	4	-0.1236260883658673
1106	5	-0.11449317376475676
1106	6	-0.14157302080150203
1106	7	-0.1874536755540248
1106	8	-0.16539645127153335
1106	9	-0.1753973878718469
1106	10-11	-0.1765474350955074
1106	12-13	-0.18962890411914657
1106	14-15	-0.19806724145652765
1106	16-17	-0.2041825488782152
1106	18-19	-0.20377778507543098
1106	20-21	-0.22726646054832145
1106	22-23	-0.23800449669084855
1106	24-25	-0.24968046389395226
1106	26-27	-0.2861636377212946
1106	28-29	-0.2966417538345354
1106	30-31	-0.28886389356762265
1106	32-33	-0.379824501040126
1106	34-35	-0.4072075285096233
1106	36-37	-0.39715953740504517
1106	38-39	-0.32119581971413425
1106	40-41	-0.3202652722043311
1106	42-43	-0.32757310909552473
1106	44-45	-0.29706145770287407
1106	46-47	-0.31923089485872325
1106	48-49	-0.4699960011681128
1106	50-51	-0.35122818316430227
1106	52-53	-0.2742662513421372
1106	54-55	-0.28450604981226135
1106	56-57	-0.3996993788787577
1106	58-59	-0.3406243083338687
1106	60-61	-0.18478815715999986
1106	62-63	-0.28609069059573855
1106	64-65	-0.2620351437599666
1106	66-67	-0.17648318605235147
1106	68-69	-0.33902939782113606
1106	70-71	-0.2589777387852088
1106	72-73	-0.2757116672178128
1106	74-75	-0.2070409160866049
1106	76-77	-0.09529495360633433
1106	78-79	-0.02855980989961182
1106	80-81	0.13641679200178203
1106	82-83	0.08392623756306605
1106	84-85	0.18516825185608
1106	86-87	0.21392078872289844
1106	88-89	0.14830199141850287
1106	90-91	0.2120028545131376
1106	92-93	0.38823105755219345
1106	94-95	0.32719066362256655
1106	96-97	0.011321869206460278
1106	98-99	-0.06888723594421897
1106	100	-0.001028431492805737
1107	1	-0.32472798187131957
1107	2	-0.2711245210834292
1107	3	-0.24345648442081824
1107	4	-0.19616024805927879
1107	5	-0.1912251036147623
1107	6	-0.23157249881872843
1107	7	-0.25533580045990334
1107	8	-0.21247784421017002
1107	9	-0.19233257246022362
1107	10-11	-0.26535561283186127
1107	12-13	-0.25874619453256287
1107	14-15	-0.33441933255139844
1107	16-17	-0.2573607812956453
1107	18-19	-0.29701709466836235
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2207	70-71	0.6174207316921176
2207	72-73	0.567044504632662
2207	74-75	0.523939490152884
2207	76-77	0.4510738177493572
2207	78-79	0.6686467138833478
2207	80-81	0.5260648782539015
2207	82-83	0.6911667843911111
2207	84-85	0.8392743152228341
2207	86-87	0.7443298850913855
2207	88-89	0.4207059006354932
2207	90-91	0.7376308678531416
2207	92-93	0.8842079236601883
2207	94-95	0.8860195854271531
2207	96-97	1.0584054803246588
2207	98-99	1.1433396768511646
2207	100	1.0629582636325043
2208	1	-0.48350897861341835
2208	2	-0.45630363853506495
2208	3	-0.3947677111015615
2208	4	-0.4089738439864732
2208	5	-0.4290613817490154
2208	6	-0.4479594113069112
2208	7	-0.3818452750166017
2208	8	-0.37010007357581287
2208	9	-0.36154484625752303
2208	10-11	-0.3303739053534045
2208	12-13	-0.2990321928604516
2208	14-15	-0.4066603436801728
2208	16-17	-0.41986100410127136
2208	18-19	-0.4114022658867498
2208	20-21	-0.3593589204533174
2208	22-23	-0.3733824312875882
2208	24-25	-0.39995843762962835
2208	26-27	-0.41573565769690646
2208	28-29	-0.4408797231086652
2208	30-31	-0.4019571979229255
2208	32-33	-0.3554306159744982
2208	34-35	-0.3708991504086967
2208	36-37	-0.2687442402215581
2208	38-39	-0.2792488911997566
2208	40-41	-0.14070507623170414
2208	42-43	-0.2143437617138204
2208	44-45	-0.09743846158958291
2208	46-47	-0.04388499395384571
2208	48-49	-0.06907793292382536
2208	50-51	-0.09845499024808291
2208	52-53	0.06675803146995918
2208	54-55	-0.019845783776027304
2208	56-57	0.09970540299652697
2208	58-59	0.18012195011041499
2208	60-61	0.13116787066961422
2208	62-63	0.17203343554998796
2208	64-65	0.21277128259949052
2208	66-67	0.3117107378548276
2208	68-69	0.33070461145824837
2208	70-71	0.23448541654629196
2208	72-73	0.16642469768257584
2208	74-75	0.1407919041325698
2208	76-77	0.08772732543576112
2208	78-79	0.16041318458715637
2208	80-81	-0.12434719054988008
2208	82-83	-0.09904879955417911
2208	84-85	-0.016955366290115848
2208	86-87	-0.149575752190934
2208	88-89	-0.3322344831006774
2208	90-91	-0.36137881305838704
2208	92-93	-0.502790705580896
2208	94-95	-0.9189234358747367
2208	96-97	-2.0817492466317447
2208	98-99	-1.4116712786574777
2208	100	-1.431049894584195
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	265461.0
3	122449.0
4	132347.0
5	101421.0
6	96974.0
7	104588.0
8	120117.0
9	133317.0
10	145556.0
11	160098.0
12	183082.0
13	231693.0
14	316164.0
15	386528.0
16	424313.0
17	404865.0
18	389160.0
19	369704.0
20	378227.0
21	400365.0
22	427561.0
23	460298.0
24	491142.0
25	523389.0
26	562562.0
27	615394.0
28	680441.0
29	767263.0
30	869239.0
31	993517.0
32	1162312.0
33	1391402.0
34	1742388.0
35	2223062.0
36	2433346.0
37	1558579.0
38	307831.0
39	7813.0
40	2.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.75508234853251	28.340216110803564	26.871569188569293	22.033132352094636
2	23.730765055797807	27.543126366234144	28.06220646811376	20.66390210985429
3	23.626660667743376	26.747441118244947	26.850669872975246	22.775228341036435
4	24.6633181193325	25.521968649504075	26.944109580654533	22.87060365050889
5	25.46120774324568	25.70192646523032	25.424882581700142	23.41198320982386
6	25.549334968435517	25.431977655696546	25.463267505255764	23.55541987061217
7	24.99216035025993	26.231841616478945	25.06065568136077	23.715342351900357
8	24.853175748725004	25.57686947760476	25.76280120226188	23.807153571408357
9	24.778984388475063	25.846223316366462	25.485195566433603	23.889596728724875
10-11	24.7277667294668	25.607010074073244	25.735219335714504	23.930003860745455
12-13	24.08539418940393	25.628798463963104	26.036530912903324	24.249276433729644
14-15	23.323896410508272	25.95918061831957	27.320410057624535	23.396512913547625
16-17	23.02127005774809	25.86501575168225	27.75353311924153	23.360181071328125
18-19	22.3215736965617	25.862225227847713	29.232899022764432	22.58330205282616
20-21	21.651355828031672	25.680223399847062	30.875708001666922	21.79271277045434
22-23	21.521838012430347	25.576164125330475	31.423947494674277	21.4780503675649
24-25	21.196627655072287	25.145382179982832	32.33419951637082	21.32379064857406
26-27	21.19683808892439	25.155616324963276	32.86628964275558	20.78125594335675
28-29	21.326097895764644	25.29655312946637	32.79025061202541	20.58709836274357
30-31	21.23505659202641	25.178993044505955	32.80256310789874	20.78338725556889
32-33	21.24084429678086	25.34605444946233	32.60760540917248	20.80549584458433
34-35	21.400548392035596	25.348588855725783	32.54399165842017	20.706871093818453
36-37	21.45793609234668	25.439152592411858	32.291311256906425	20.81160005833503
38-39	21.572920645294996	25.602127861281186	31.919816619522575	20.905134873901243
40-41	21.629990102885298	25.91639558906263	31.383546716448624	21.070067591603443
42-43	21.778074299046228	26.113506874352915	30.67143002634683	21.436988800254024
44-45	21.921336944807997	26.493100150677364	29.93937493665927	21.646187967855376
46-47	22.15937833732977	26.870975307376803	29.329915014033453	21.63973134125997
48-49	22.321385450516246	27.097474357746176	28.68689412368795	21.89424606804963
50-51	22.2977246463789	27.375596889357883	27.993994845544062	22.332683618719155
52-53	22.584088906311635	27.62061015056185	27.740409911618926	22.054891031507594
54-55	22.446862399338766	27.730420104549818	27.512366281336664	22.31035121477475
56-57	22.453952598818198	27.965125517174172	27.37164283665911	22.209279047348517
58-59	22.676223018977364	28.16834148036684	27.0994736569338	22.055961843721995
60-61	22.54760333819801	28.297692947558257	26.8534873431689	22.301216371074833
62-63	22.623160040468218	28.53793524233611	26.69839683941433	22.14050787778134
64-65	22.608690178834657	28.990159387862292	26.289355390059693	22.11179504324336
66-67	22.56498759255377	29.204508119174065	26.00720910901429	22.223295179257878
68-69	22.52464568659306	29.403317577769066	25.81391981631728	22.25811691932059
70-71	22.61639107883397	29.475875756081336	25.62152536887844	22.286207796206252
72-73	22.63956410864607	29.429710291214022	25.395855460148546	22.534870139991362
74-75	22.74945865900493	29.508568680484228	25.20920436487204	22.532768295638807
76-77	22.149158369169516	30.03262785833393	24.883828549120185	22.934385223376367
78-79	22.396251149592604	29.91763100215946	24.68098168427107	23.00513616397686
80-81	22.344004361388237	29.978838355165404	24.41717399413129	23.259983289315063
82-83	22.33847276050616	29.91695817604823	24.3050841785628	23.439484884882805
84-85	22.304958034715682	29.956571490985095	24.105690084050572	23.63278039024865
86-87	22.26379999433514	30.11442540090256	23.86075368574146	23.761020919020837
88-89	22.422646211439595	30.087913437920072	23.643423796765322	23.846016553875014
90-91	22.16185031639969	29.962286250242855	23.55147859032133	24.324384843036125
92-93	22.225108758160385	30.046494010254264	23.368032071630815	24.360365159954533
94-95	22.170224230526713	30.009377753642376	23.152537923926477	24.66786009190443
96-97	22.073914645765036	29.926590308389994	22.85677926427274	25.142715781572228
98-99	22.019931771837598	29.970220094133577	22.7435307224871	25.26631741154172
100	22.87430737914251	30.34802757322354	22.025961467305052	24.751703580328893
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9683.0
1	7129.5
2	3690.0
3	2564.0
4	2332.0
5	2540.5
6	2829.5
7	3205.5
8	3809.5
9	4490.0
10	5475.0
11	6949.5
12	8939.0
13	12537.0
14	20781.0
15	30717.5
16	56824.5
17	69527.0
18	58654.5
19	59080.0
20	79816.0
21	92364.5
22	90634.5
23	99705.5
24	109222.5
25	118226.5
26	127860.0
27	141853.5
28	160284.0
29	179140.0
30	199087.0
31	224598.0
32	257543.5
33	294731.5
34	336884.5
35	387397.0
36	444391.0
37	512713.0
38	582658.5
39	643771.5
40	694691.5
41	750044.0
42	835909.0
43	921334.0
44	984151.5
45	1034014.5
46	1038459.0
47	993928.5
48	950327.0
49	934377.0
50	909961.0
51	841747.5
52	747530.0
53	652574.5
54	569192.5
55	501975.0
56	438925.5
57	379080.5
58	335409.0
59	300157.5
60	263951.5
61	228571.0
62	198527.0
63	174113.0
64	152337.0
65	131882.0
66	113182.0
67	95752.0
68	79771.5
69	66541.0
70	55689.5
71	46387.5
72	39198.0
73	33445.5
74	28405.0
75	23781.5
76	19456.0
77	15780.5
78	12769.0
79	10267.5
80	8099.5
81	6197.0
82	4732.0
83	3499.0
84	2479.5
85	1742.5
86	1232.0
87	828.0
88	546.0
89	386.5
90	297.0
91	212.5
92	123.5
93	77.0
94	51.0
95	34.5
96	19.0
97	10.5
98	5.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02020922868487867
2	0.008282025378589086
3	3.169719937130869E-5
4	0.0016482543673080519
5	4.70929819230872E-4
6	5.931904453773483E-4
7	0.003228586164534728
8	0.002694261946561239
9	0.0015803317972266763
10-11	6.882820434912745E-4
12-13	5.682855030141772E-4
14-15	6.656411867974826E-4
16-17	0.00134486688761124
18-19	8.852574967272641E-4
20-21	7.199792428625832E-4
22-23	5.207397039572142E-4
24-25	4.890425045859056E-4
26-27	0.0022188039559916085
28-29	0.00241125123788884
30-31	0.0035297095585621607
32-33	0.0033508467906812043
34-35	0.003907811865348486
36-37	0.005363618950759306
38-39	0.005159851240515179
40-41	0.005757569857231286
42-43	0.005601347946044122
44-45	0.0046368474508885855
46-47	0.006262460961502846
48-49	0.005123625869805112
50-51	0.00426100922977164
52-53	0.0020535257021269274
54-55	0.001953905932674243
56-57	0.0011727963767384215
58-59	0.0016731593096712231
60-61	0.002800673973022061
62-63	0.002119184186538924
64-65	0.0028912373997972284
66-67	0.002981800826572396
68-69	0.0010754406929551162
70-71	0.0035342377299009188
72-73	0.004691185506953686
74-75	0.00487910461751216
76-77	0.005918319939757209
78-79	0.005193812525555867
80-81	0.004949291273262914
82-83	0.0048066538760920255
84-85	0.004054977433858133
86-87	0.002062582044804444
88-89	0.0011705322910690423
90-91	5.18475618287835E-4
92-93	0.011426840373356784
94-95	0.01353470413154881
96-97	0.012486432466626247
98-99	0.01650518452977431
100	0.01679045932411609
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.208397E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.91653454679069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.3525698565557	29.219023857231857
2	10.110095952560378	7.262392211030072
3	3.1084703102384164	3.3493644385605377
4	1.3844972390374004	1.989053716632925
5	0.7824904713885592	1.405217302408086
6	0.48835523297365224	1.0524016557722515
7	0.3347961478047687	0.8417302186133692
8	0.2602594750345736	0.7478094740967097
9	0.21201178537369078	0.6853255752330845
>10	1.5220587256828293	10.344334261734799
>50	0.13054689639883538	3.3637213212303694
>100	0.26194195203453785	22.449769000168114
>500	0.04477870511034622	10.867511326079141
>1k	0.0066979117445990525	3.1164661226286263
>5k	1.5153108062983457E-4	0.3364036859731431
>10k+	2.7780698115469635E-4	2.969475832606738
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	90992	0.41202736645630295	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	85141	0.38553303595322763	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	68817	0.3116151670193358	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53040	0.2401742078077447	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43681	0.197795052248305	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34959	0.15830034183165437	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32100	0.14535429997414415	No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27069	0.12257306996885071	No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26540	0.12017766733064754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001258831632174831	0.0	0.0	0.0	0.0
2	0.0012678879748523476	0.0	0.0	0.0	0.0
3	0.001272416146191106	0.0	0.0	0.0	0.0
4	0.001272416146191106	0.0	0.0	0.0	0.0
5	0.0012860006602073812	0.0	0.0	0.0	0.0
6	0.001295057002884898	0.0	0.0	0.0	4.528171338758384E-6
7	0.0013041133455624148	0.0	0.0	0.0	4.528171338758384E-6
8	0.0013041133455624148	0.0	0.0	0.0	4.528171338758384E-6
9	0.00131769785957869	0.0	0.0	0.0	4.528171338758384E-6
10-11	0.0014716556850964749	0.0	0.0	0.0	4.528171338758384E-6
12-13	0.001883719276923488	4.528171338758384E-6	0.0	0.0	4.528171338758384E-6
14-15	0.0029614240555479836	4.528171338758384E-6	0.0	0.0	4.528171338758384E-6
16-17	0.005338714008396135	9.056342677516769E-6	0.0	0.0	4.528171338758384E-6
18-19	0.00888653625231333	9.056342677516769E-6	0.0	0.0	4.528171338758384E-6
20-21	0.013862996553608793	9.056342677516769E-6	0.0	0.0	4.528171338758384E-6
22-23	0.020082439887393437	9.056342677516769E-6	0.0	0.0	4.528171338758384E-6
24-25	0.027889007275412887	9.056342677516769E-6	0.0	0.0	4.528171338758384E-6
26-27	0.036472156048029405	9.056342677516769E-6	0.0	0.0	4.528171338758384E-6
28-29	0.047665795597440136	9.056342677516769E-6	0.0	0.0	4.528171338758384E-6
30-31	0.05676741998834449	9.056342677516769E-6	0.0	0.0	9.056342677516769E-6
32-33	0.0628691308673214	9.056342677516769E-6	0.0	0.0	9.056342677516769E-6
34-35	0.06896404948929019	1.5848599685654346E-5	0.0	0.0	1.3584514016275154E-5
36-37	0.07545291901773096	1.8112685355033537E-5	0.0	0.0	1.3584514016275154E-5
38-39	0.08100219299337935	2.2640856693791922E-5	0.0	0.0	1.3584514016275154E-5
40-41	0.08587450535388338	2.2640856693791922E-5	0.0	0.0	1.3584514016275154E-5
42-43	0.09077625082808932	2.4904942363171117E-5	0.0	0.0	1.5848599685654346E-5
44-45	0.09606968312309788	2.94331137019295E-5	0.0	0.0	1.8112685355033537E-5
46-47	0.10204913337592833	3.6225370710067074E-5	0.0	0.0	1.8112685355033537E-5
48-49	0.10805122448545257	3.6225370710067074E-5	0.0	0.0	1.8112685355033537E-5
50-51	0.1147189567817743	3.6225370710067074E-5	0.0	2.264085669379192E-6	1.8112685355033537E-5
52-53	0.12248477062774492	3.6225370710067074E-5	0.0	4.528171338758384E-6	1.8112685355033537E-5
54-55	0.1314754548208497	3.6225370710067074E-5	0.0	4.528171338758384E-6	1.8112685355033537E-5
56-57	0.1426826788842767	3.6225370710067074E-5	0.0	4.528171338758384E-6	1.8112685355033537E-5
58-59	0.1556513615984807	3.6225370710067074E-5	4.528171338758384E-6	4.528171338758384E-6	1.8112685355033537E-5
60-61	0.1704041438201555	4.075354204882546E-5	4.528171338758384E-6	4.528171338758384E-6	1.8112685355033537E-5
62-63	0.18801646624225626	4.075354204882546E-5	4.528171338758384E-6	9.056342677516769E-6	1.8112685355033537E-5
64-65	0.20833210695359575	4.5281713387583845E-5	4.528171338758384E-6	9.056342677516769E-6	1.8112685355033537E-5
66-67	0.2321344395957792	4.5281713387583845E-5	4.528171338758384E-6	1.3584514016275154E-5	1.8112685355033537E-5
68-69	0.26018872512505675	4.5281713387583845E-5	4.528171338758384E-6	1.3584514016275154E-5	1.8112685355033537E-5
70-71	0.29298627013168377	4.754579905696303E-5	4.528171338758384E-6	1.8112685355033537E-5	1.8112685355033537E-5
72-73	0.33153006456719514	4.9809884726342226E-5	4.528171338758384E-6	1.8112685355033537E-5	1.8112685355033537E-5
74-75	0.3762887741651524	4.9809884726342226E-5	4.528171338758384E-6	1.8112685355033537E-5	1.8112685355033537E-5
76-77	0.42797332182574055	4.9809884726342226E-5	4.528171338758384E-6	1.8112685355033537E-5	1.8112685355033537E-5
78-79	0.4855399640553759	4.9809884726342226E-5	4.528171338758384E-6	1.8112685355033537E-5	2.2640856693791922E-5
80-81	0.5501162155174093	5.4338056065100615E-5	6.792257008137576E-6	2.2640856693791922E-5	2.2640856693791922E-5
82-83	0.62177679103893	5.4338056065100615E-5	1.3584514016275154E-5	2.2640856693791922E-5	2.2640856693791922E-5
84-85	0.7010379021525568	5.4338056065100615E-5	1.3584514016275154E-5	3.169719937130869E-5	2.2640856693791922E-5
86-87	0.7869576892198278	5.4338056065100615E-5	1.3584514016275154E-5	3.169719937130869E-5	2.2640856693791922E-5
88	0.8567436018071026	5.4338056065100615E-5	1.3584514016275154E-5	3.169719937130869E-5	2.2640856693791922E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	21620	0.0	60.74253	2
ATATCTC	23750	0.0	56.235737	1
ATCTCGA	30725	0.0	42.41757	3
ATGGATC	27225	0.0	40.913647	3
TATGGAT	27785	0.0	40.464386	2
ATATGGA	28805	0.0	39.332718	1
TCTCGAG	34745	0.0	37.144623	4
GGATCCG	29300	0.0	36.957756	5
TGGATCC	30830	0.0	36.038086	4
CTCGAGG	41660	0.0	30.990608	5
GATCCGG	44825	0.0	29.376333	1
TCGAGGG	46855	0.0	27.464092	6
ATCCGGC	55185	0.0	23.703602	2
CGAGGGC	60645	0.0	21.071817	7
TCCGGCG	67980	0.0	19.295961	3
AGGGCGC	73165	0.0	17.247484	9
ATCCCGC	3995	0.0	16.58785	6
GAGGGCG	78410	0.0	16.261631	8
GCGCCGT	80375	0.0	16.080387	9
CGGCGCG	81365	0.0	15.954263	5
>>END_MODULE