FastQCFastQC Report
Wed 25 May 2016
SRR491019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18049650
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA243700.1350164684633774No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197740.10955337084098585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA115950.043.7892761
TCTCGAG140500.036.0970762
GATCCGG188600.029.389141
CTCGAGG183350.027.6328873
TCGAGGG212700.023.687314
ATCCGGC237050.023.55612
TATCTCG78050.021.1378652
TCCGGCG280700.019.8410363
GCGCCGT308800.017.882819
CGGCGCG325500.016.9368655
ATATCTC101050.016.9347921
CGAGGGC314200.016.2147065
GGATCCG126300.015.5546893
CCGGCGC360350.015.455474
CGTCGAC296200.015.25578612-13
TCGACTT306350.014.76567314-15
GCCGTCG308650.014.68603310-11
AGGGCGC344050.014.6029627
ATGGATC135700.014.5160671
GAGGGCG388000.013.1547596
Produced by FastQC (version 0.11.5)