FastQCFastQC Report
Wed 25 May 2016
SRR491018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26834187
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA170900.044.833721
TCTCGAG205900.037.0267682
CTCGAGG259550.029.2826463
GATCCGG256100.027.6776641
TCGAGGG305200.024.8410264
TATCTCG100050.023.7713992
ATCCGGC311350.022.7502252
TCCGGCG400900.017.7270743
GGATCCG166700.017.6506252
GCGCCGT409050.017.395179
CGAGGGC450350.017.0955965
ATATCTC150800.016.2815171
AGGGCGC471200.015.9882237
CGGCGCG444000.015.9109655
ATGGATC182350.015.3216811
CGTCGAC390400.014.99382112-13
CCGGCGC490900.014.5344444
TGGATCC210750.014.4398411
GCCGTCG416150.014.21286910-11
GGGCGCG532550.014.1993218
Produced by FastQC (version 0.11.5)