##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491018_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26834187
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.313658244984282	33.0	31.0	34.0	28.0	34.0
2	31.599848953873654	34.0	31.0	34.0	28.0	34.0
3	31.658964290589463	34.0	31.0	34.0	28.0	34.0
4	35.068309168449936	37.0	35.0	37.0	32.0	37.0
5	34.79309796119406	37.0	35.0	37.0	32.0	37.0
6	34.74168529868261	37.0	35.0	37.0	32.0	37.0
7	34.66370682294195	37.0	35.0	37.0	32.0	37.0
8	34.565256476747365	37.0	35.0	37.0	31.0	37.0
9	36.0481947151967	39.0	37.0	39.0	31.0	39.0
10-11	35.9034817973058	39.0	37.0	39.0	30.0	39.0
12-13	35.682343776616	39.0	36.0	39.0	30.0	39.0
14-15	36.94821879641816	40.0	37.0	41.0	31.0	41.0
16-17	36.863974395795935	40.0	37.0	41.0	30.0	41.0
18-19	36.74276399728451	40.0	37.0	41.0	30.0	41.0
20-21	36.60094760836242	40.0	37.0	41.0	30.0	41.0
22-23	36.41528530378058	40.0	37.0	41.0	29.0	41.0
24-25	36.193111067609394	40.0	36.5	41.0	27.5	41.0
26-27	35.851240471716174	40.0	36.0	41.0	27.0	41.0
28-29	35.5282524676451	39.0	35.0	41.0	26.5	41.0
30-31	35.255703275079654	39.0	35.0	41.0	25.0	41.0
32-33	35.00440782871492	39.0	35.0	41.0	24.0	41.0
34-35	34.72948239497623	39.0	35.0	40.0	23.5	41.0
36-37	34.45389331154322	39.0	34.5	40.0	21.5	41.0
38-39	34.09674319553635	38.0	34.0	40.0	17.0	41.0
40-41	33.665221383453876	38.0	33.0	40.0	13.5	41.0
42-43	33.20087605039049	38.0	33.0	40.0	8.5	41.0
44-45	32.714649487983365	38.0	32.5	40.0	2.0	41.0
46-47	32.19044717099125	38.0	31.5	40.0	2.0	41.0
48-49	31.76080870271941	37.5	31.0	40.0	2.0	41.0
50-51	31.2515254514698	37.0	30.5	40.0	2.0	41.0
52-53	30.71893022136277	36.5	29.5	39.5	2.0	41.0
54-55	30.199968569944005	36.0	28.0	39.0	2.0	40.0
56-57	29.596784262552838	35.5	27.5	39.0	2.0	40.0
58-59	28.992128753518784	35.0	26.0	38.5	2.0	40.0
60-61	28.405097665153782	34.5	25.5	38.0	2.0	40.0
62-63	27.79419896343422	34.0	23.0	38.0	2.0	40.0
64-65	27.143005208244247	34.0	21.5	37.5	2.0	39.5
66-67	26.48535098156691	33.0	19.0	37.0	2.0	39.0
68-69	25.837398464876166	32.5	15.0	36.0	2.0	39.0
70-71	25.064214913610016	31.5	7.5	35.5	2.0	38.5
72-73	24.345395521019512	31.0	4.0	35.0	2.0	38.0
74-75	23.637859719767174	30.5	2.0	35.0	2.0	37.0
76-77	22.65073517971683	29.5	2.0	34.0	2.0	36.0
78-79	22.687121730201852	30.0	2.0	34.0	2.0	36.0
80-81	22.108506864769183	29.5	2.0	34.0	2.0	35.5
82-83	21.22108415283832	29.0	2.0	34.0	2.0	35.0
84-85	20.376839570358513	27.5	2.0	33.5	2.0	35.0
86-87	19.778171423639552	26.0	2.0	33.0	2.0	35.0
88-89	19.12714855121193	25.5	2.0	32.5	2.0	34.5
90-91	18.362792004840692	25.0	2.0	32.0	2.0	34.0
92-93	17.64836795689022	23.5	2.0	32.0	2.0	34.0
94-95	16.786024614794552	19.0	2.0	32.0	2.0	34.0
96-97	15.704331791382387	4.5	2.0	31.0	2.0	34.0
98-99	14.657261276445603	2.0	2.0	31.0	2.0	34.0
100	13.868519772929957	2.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.27251477174750605
1101	2	0.2756452932384832
1101	3	0.27360487038233927
1101	4	0.2711858673114875
1101	5	0.3438798780689538
1101	6	0.2846228523915997
1101	7	0.3143718649284608
1101	8	0.3034698173661141
1101	9	0.3754125622777664
1101	10-11	0.35270900878904143
1101	12-13	0.36176100868937056
1101	14-15	0.4176901746212991
1101	16-17	0.40862190440380886
1101	18-19	0.35746065860922727
1101	20-21	0.3876064185602175
1101	22-23	0.3737097390106996
1101	24-25	0.3924953227116035
1101	26-27	0.32859212462728493
1101	28-29	0.37107588392247237
1101	30-31	0.3776825442298417
1101	32-33	0.31216441442662557
1101	34-35	0.2887765241835254
1101	36-37	0.287324199241354
1101	38-39	0.2769557650774459
1101	40-41	0.19357193474074563
1101	42-43	0.22669597574238765
1101	44-45	0.18871394013014253
1101	46-47	0.13686681753654284
1101	48-49	0.11506886293788199
1101	50-51	0.07340985227803287
1101	52-53	0.04286814458433952
1101	54-55	0.004825747587396734
1101	56-57	0.06336923415122797
1101	58-59	-0.008344616639440972
1101	60-61	-0.07536100158520753
1101	62-63	-0.07484507732063861
1101	64-65	-0.12437353145081431
1101	66-67	-0.33379963738951446
1101	68-69	-0.3299373001533823
1101	70-71	-0.28243416934300214
1101	72-73	-0.2624309752262519
1101	74-75	-0.3225440199177072
1101	76-77	-0.21781627228614298
1101	78-79	-0.1614283397195635
1101	80-81	-0.24625517837058908
1101	82-83	-0.08311156957835308
1101	84-85	0.10031667048613002
1101	86-87	-0.28408740711742553
1101	88-89	-0.5234613890773332
1101	90-91	-0.6335901755825333
1101	92-93	-0.6480149446721413
1101	94-95	-0.44595861417557714
1101	96-97	-0.144108184110749
1101	98-99	-0.05111074817607886
1101	100	0.09301950209112064
1102	1	0.1687475523330484
1102	2	0.16484161650064522
1102	3	0.1835487352496763
1102	4	0.20027527894951902
1102	5	0.1988872581799086
1102	6	0.2150488202230676
1102	7	0.2057922770814784
1102	8	0.20294767028495642
1102	9	0.24485249797795205
1102	10-11	0.22323000014402083
1102	12-13	0.22522735305061303
1102	14-15	0.2718874546643235
1102	16-17	0.28906650388652366
1102	18-19	0.2221726129681656
1102	20-21	0.2507532441433753
1102	22-23	0.2535676818733137
1102	24-25	0.2426829258198282
1102	26-27	0.2635210898870213
1102	28-29	0.22640226105844619
1102	30-31	0.22249152644180725
1102	32-33	0.24469318350290337
1102	34-35	0.1776856129473927
1102	36-37	0.19918623402787716
1102	38-39	0.1370804448730567
1102	40-41	0.16125589242277272
1102	42-43	0.20172096221669733
1102	44-45	0.10727485261288194
1102	46-47	0.05294998882458657
1102	48-49	0.007273945625609457
1102	50-51	0.007937437697094651
1102	52-53	-0.03594109643440646
1102	54-55	-0.01766596791169306
1102	56-57	-0.0036912151355181777
1102	58-59	-0.11707099281383293
1102	60-61	-0.06000021575048464
1102	62-63	-0.2735311343796418
1102	64-65	-0.23767461268272072
1102	66-67	-0.39922321146316264
1102	68-69	-0.4071002086456126
1102	70-71	-0.23702128544000445
1102	72-73	-0.26049079142385523
1102	74-75	-0.3623920998146595
1102	76-77	-0.22392930132489397
1102	78-79	-0.09129925418280394
1102	80-81	-0.17243675611479148
1102	82-83	0.026085814060689216
1102	84-85	0.23603716102715566
1102	86-87	-0.1514387256564298
1102	88-89	-0.2689256686223658
1102	90-91	-0.44223361403962613
1102	92-93	-0.5882940834277122
1102	94-95	-0.3823273567661971
1102	96-97	-0.08262101677845735
1102	98-99	-8.533921261335564E-4
1102	100	0.026227336023758596
1103	1	0.06062302844832601
1103	2	0.06526423269610149
1103	3	0.010843334277744532
1103	4	0.05501833071547679
1103	5	0.15522389365318645
1103	6	0.049832899791027785
1103	7	0.10768458194909414
1103	8	0.11312716504463793
1103	9	0.08303091074154167
1103	10-11	0.09616010481273207
1103	12-13	0.056491690548561735
1103	14-15	0.012283912131451302
1103	16-17	0.112671968906632
1103	18-19	0.10841910326088566
1103	20-21	0.11884390305834103
1103	22-23	0.11136072381713547
1103	24-25	0.07964370434783774
1103	26-27	0.11047307661571892
1103	28-29	0.1504590395270995
1103	30-31	0.05670693869962662
1103	32-33	-0.11203953256046617
1103	34-35	0.1027121241206359
1103	36-37	0.011341270761178635
1103	38-39	0.007206999571238271
1103	40-41	-0.01555555835209077
1103	42-43	0.05718707247761046
1103	44-45	0.03396095293454948
1103	46-47	0.03469202140168193
1103	48-49	-0.037348488308797556
1103	50-51	-0.06028880314324425
1103	52-53	-0.037155289693338034
1103	54-55	-0.2339117340133754
1103	56-57	-0.10879487514198516
1103	58-59	-0.2726151148334779
1103	60-61	-0.28242048447500423
1103	62-63	-0.21295678015984976
1103	64-65	-0.15318994047008871
1103	66-67	-0.3028422805666082
1103	68-69	-0.21444218131926363
1103	70-71	-0.12581287097074068
1103	72-73	-0.16627846196043805
1103	74-75	-0.27192326194751715
1103	76-77	-0.1349874861828546
1103	78-79	-0.06901427567457574
1103	80-81	-0.15353034414401634
1103	82-83	0.08373392229043475
1103	84-85	0.3588230910763137
1103	86-87	-0.0027090511586003174
1103	88-89	-0.1660240707885805
1103	90-91	-0.5004106326067905
1103	92-93	-0.7797674398841039
1103	94-95	-0.5399728576643561
1103	96-97	-0.3036858441612367
1103	98-99	-0.49832433723689995
1103	100	-0.4513546423880701
1104	1	-0.02522427509970626
1104	2	-0.00182049168253684
1104	3	0.054355470022173336
1104	4	0.015146444174703788
1104	5	0.051920710160999306
1104	6	0.006075878742620944
1104	7	0.014341909167050915
1104	8	-0.033182626848415
1104	9	-0.03529396075586533
1104	10-11	-0.042807877929327276
1104	12-13	0.003321926401071096
1104	14-15	0.06248273806026816
1104	16-17	0.028234323715693677
1104	18-19	0.05077777010808404
1104	20-21	0.06025442927051472
1104	22-23	0.05977878952571558
1104	24-25	0.10466655436461991
1104	26-27	0.09381742107316882
1104	28-29	0.08036170517990371
1104	30-31	0.09321497466405759
1104	32-33	0.14454189869414336
1104	34-35	0.17650823726248177
1104	36-37	0.1023420501067207
1104	38-39	0.14025800251181408
1104	40-41	0.022830881406051162
1104	42-43	0.10314241068465435
1104	44-45	0.09377430557275801
1104	46-47	0.11319900311177378
1104	48-49	0.03176832466557755
1104	50-51	0.06706960879810708
1104	52-53	0.11313678816191342
1104	54-55	0.02042807449145201
1104	56-57	0.20336784128378937
1104	58-59	0.1835795620777283
1104	60-61	0.12081296809140696
1104	62-63	0.14224140717509215
1104	64-65	0.12536745631769008
1104	66-67	0.08984360075724496
1104	68-69	0.10041575152216353
1104	70-71	0.17770745363342755
1104	72-73	0.21978468644983806
1104	74-75	0.2153888775569044
1104	76-77	0.28073085211722315
1104	78-79	0.3347161086038781
1104	80-81	0.2538080087008012
1104	82-83	0.4443351483182987
1104	84-85	0.6719543415072238
1104	86-87	0.379563575460633
1104	88-89	0.23073314092037833
1104	90-91	-0.044522459289840555
1104	92-93	-0.16332719179270327
1104	94-95	0.07703699399393571
1104	96-97	0.33389861761785866
1104	98-99	0.31963947743089527
1104	100	0.23833439081873742
1105	1	-0.044462191390554295
1105	2	-0.010348956621836436
1105	3	0.007102059220013501
1105	4	-0.03809442635700577
1105	5	0.011317403110425062
1105	6	-0.027056573552108887
1105	7	-0.03999533401676558
1105	8	-0.038345313993460195
1105	9	-0.022791366299721005
1105	10-11	-0.03334054638803963
1105	12-13	-0.011366862867831173
1105	14-15	-0.004576935154929629
1105	16-17	0.002484738754425564
1105	18-19	0.014861831382773971
1105	20-21	-0.031165495491990214
1105	22-23	-0.02320020308724935
1105	24-25	-0.02431937006698348
1105	26-27	-0.014045330204389472
1105	28-29	0.039018448481243695
1105	30-31	0.025921655752163986
1105	32-33	0.04901642204872303
1105	34-35	-0.047974707732166166
1105	36-37	0.0058214716748139494
1105	38-39	0.00418648237542385
1105	40-41	0.02184851996038617
1105	42-43	0.015563039157726166
1105	44-45	-0.03021173394410681
1105	46-47	-0.00372179634457126
1105	48-49	0.019371452444605808
1105	50-51	-0.02689839392722959
1105	52-53	0.05316098831983851
1105	54-55	-0.01746003611644298
1105	56-57	0.1509828064485923
1105	58-59	0.1662349611306091
1105	60-61	-0.05449722807745516
1105	62-63	-0.013350267916301561
1105	64-65	0.15167896899330557
1105	66-67	0.15366652709937867
1105	68-69	0.08474420621298862
1105	70-71	0.21039189542971215
1105	72-73	0.26359371852896984
1105	74-75	0.28340369595469994
1105	76-77	0.3153069328857043
1105	78-79	0.41534530808363357
1105	80-81	0.2737744456117639
1105	82-83	0.6567333149310706
1105	84-85	1.0644153900996614
1105	86-87	0.6598036105757821
1105	88-89	0.537006899898337
1105	90-91	0.40145490067803635
1105	92-93	0.24204647329288065
1105	94-95	0.10547919172254083
1105	96-97	0.4470327482936689
1105	98-99	0.5340719860401162
1105	100	0.4523842939187226
1106	1	-0.2863279023068017
1106	2	-0.34147729492110557
1106	3	-0.2816974026709502
1106	4	-0.24803725628471085
1106	5	-0.24774634782485094
1106	6	-0.36306116617871
1106	7	-0.3425693667248595
1106	8	-0.3852997663720217
1106	9	-0.38441837593829575
1106	10-11	-0.40606158496177613
1106	12-13	-0.4559261526621867
1106	14-15	-0.46061708048582517
1106	16-17	-0.4295924762960581
1106	18-19	-0.4335380605346657
1106	20-21	-0.4896038401552616
1106	22-23	-0.5260830655113651
1106	24-25	-0.5157420944864484
1106	26-27	-0.49139210119825094
1106	28-29	-0.5230396075991095
1106	30-31	-0.5628858076772829
1106	32-33	-0.5020531273427764
1106	34-35	-0.6624830795745709
1106	36-37	-0.6461451974521992
1106	38-39	-0.6054537287716357
1106	40-41	-0.6191442184950091
1106	42-43	-0.589846915012636
1106	44-45	-0.599352702834473
1106	46-47	-0.6515869527956113
1106	48-49	-0.6671029558066088
1106	50-51	-0.6582042539830937
1106	52-53	-0.5411305294103244
1106	54-55	-0.701048977692011
1106	56-57	-0.5768537391380413
1106	58-59	-0.6048798392991799
1106	60-61	-0.6595780726388227
1106	62-63	-0.59120471104141
1106	64-65	-0.5282389783297319
1106	66-67	-0.5186052200787685
1106	68-69	-0.5159406337147985
1106	70-71	-0.347770011809704
1106	72-73	-0.4464530551218928
1106	74-75	-0.5056908126364483
1106	76-77	-0.37522230980323457
1106	78-79	-0.3418313971277165
1106	80-81	-0.37095464894002106
1106	82-83	-0.013786265326320546
1106	84-85	0.47131979336846186
1106	86-87	0.17288056337482516
1106	88-89	0.15202996728709195
1106	90-91	0.0439484827309542
1106	92-93	-0.0830374384963335
1106	94-95	-0.14746694914420644
1106	96-97	0.14231649723258855
1106	98-99	0.1788748788861927
1106	100	0.0968750696535654
1107	1	-0.3237473750766888
1107	2	-0.3102111129285383
1107	3	-0.3169731868984549
1107	4	-0.28057760643920204
1107	5	-0.3533768239497874
1107	6	-0.361820708355836
1107	7	-0.45462355763946505
1107	8	-0.4153337193371911
1107	9	-0.4476672424446164
1107	10-11	-0.3884380074471707
1107	12-13	-0.4003829878065517
1107	14-15	-0.4556001739076976
1107	16-17	-0.5250642407310764
1107	18-19	-0.4761046287575539
1107	20-21	-0.45098287348309185
1107	22-23	-0.44798223258714387
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2207	88-89	0.7836353223154084
2207	90-91	1.0804506822928808
2207	92-93	0.888024793460815
2207	94-95	0.7222363482557945
2207	96-97	0.6659757584006591
2207	98-99	0.5998045522139233
2207	100	0.4712576891262845
2208	1	-0.5008299642388856
2208	2	-0.4616591958830121
2208	3	-0.8522088089162878
2208	4	-0.6000917823176906
2208	5	-1.1297428693075133
2208	6	-0.7338832816443386
2208	7	-0.5996293712986116
2208	8	-0.6442426918841377
2208	9	-0.5621036264893107
2208	10-11	-0.5309905517426472
2208	12-13	-0.4968206693782946
2208	14-15	-0.6645626400022522
2208	16-17	-0.5853707449912093
2208	18-19	-0.6258467716989173
2208	20-21	-0.7168103831847006
2208	22-23	-0.6824914310842374
2208	24-25	-0.6546598232873251
2208	26-27	-0.7041043751397495
2208	28-29	-0.7161344770148546
2208	30-31	-0.7183127033841217
2208	32-33	-0.7461421028310582
2208	34-35	-0.792934214547131
2208	36-37	-0.6944437273817243
2208	38-39	-0.6842248387638961
2208	40-41	-0.7439212872317
2208	42-43	-0.7919895336246867
2208	44-45	-0.7764251914627458
2208	46-47	-0.600318359673075
2208	48-49	-0.6695407369777122
2208	50-51	-0.645149629516439
2208	52-53	-0.49167942278315024
2208	54-55	-0.43968420393078844
2208	56-57	-0.6535924800299888
2208	58-59	-0.5921008553186127
2208	60-61	-0.46839563902734227
2208	62-63	-0.49890302046710033
2208	64-65	-0.6958818502219053
2208	66-67	-0.2122102824801182
2208	68-69	-0.30735318758387464
2208	70-71	-0.15803223485352547
2208	72-73	-0.16834928262296245
2208	74-75	-0.2001244244047129
2208	76-77	-0.28897394808344856
2208	78-79	-0.434410485741374
2208	80-81	-0.4150930382721256
2208	82-83	-0.331094480633336
2208	84-85	-0.23630709711196118
2208	86-87	-0.507095778808857
2208	88-89	-1.1927530891060982
2208	90-91	-2.360846347371197
2208	92-93	-2.524211010831676
2208	94-95	-2.6567393310222975
2208	96-97	-3.4645552804706004
2208	98-99	-3.4616999780524473
2208	100	-3.321923539859041
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	438553.0
3	186771.0
4	168568.0
5	136507.0
6	127295.0
7	132520.0
8	141279.0
9	159392.0
10	183174.0
11	205639.0
12	229988.0
13	264770.0
14	318112.0
15	392153.0
16	455869.0
17	459077.0
18	431378.0
19	413250.0
20	422205.0
21	440420.0
22	462229.0
23	489093.0
24	519807.0
25	556762.0
26	601999.0
27	658373.0
28	735524.0
29	832853.0
30	950580.0
31	1087392.0
32	1278017.0
33	1548000.0
34	1968885.0
35	2611954.0
36	3237333.0
37	2728984.0
38	830746.0
39	28721.0
40	15.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.579412318182868	28.073699986000083	28.088732929814462	22.258154766002587
2	23.655966311033247	27.91227950793812	27.323552873934766	21.108201307093868
3	23.28948181415096	26.544194903414287	27.13643860602225	23.029884676412504
4	24.216529822343492	26.174259642808778	26.79420866148804	22.81500187335969
5	24.476211411122385	26.379046578051206	26.541397187985016	22.603344822841386
6	24.46436152388539	26.236040918614734	25.984913101755737	23.314684455744146
7	24.411977342177835	26.565726317358575	26.15058507863159	22.871711261832004
8	23.877212758949053	26.408094091951913	26.495318958260967	23.219374190838067
9	23.822296164909616	26.117258122867725	26.618223390955265	23.442222321267394
10-11	23.766975108481514	26.315111430363974	26.54771961454161	23.370193846612906
12-13	23.50758903625693	26.35703583329124	26.67054505960756	23.464830070844272
14-15	23.226625143589285	26.609670631903914	27.101168079713755	23.062536144793043
16-17	23.07247363448073	26.52147278233288	27.288717018773063	23.117336564413336
18-19	22.716198648856732	26.485293150925067	27.897968052964124	22.900540147254077
20-21	22.418753198348195	26.248661966886687	28.633096227629824	22.699488607135294
22-23	22.465203861382008	26.202056174720646	28.955544639373226	22.37719532452412
24-25	22.387076381360096	26.068331816554984	29.34555506550148	22.199036736583444
26-27	22.423228144166526	26.11611258434309	29.36657401811338	22.094085253377006
28-29	22.48132458281362	26.17895386583945	29.31920548449113	22.020516066855805
30-31	22.454888975006245	26.108838491561304	29.29584254530872	22.14042998812373
32-33	22.48058046252715	26.193264211805634	29.18079463335334	22.145360692313876
34-35	22.567341172142484	26.218848589287884	29.192068040988623	22.02174219758101
36-37	22.569382289128054	26.203946214032637	29.077126288512122	22.149545208327186
38-39	22.59189601467253	26.297417127207968	28.948760650327166	22.161926207792337
40-41	22.65361455217489	26.413689482153497	28.751211680338535	22.18148428533308
42-43	22.77866473200875	26.47553224303365	28.36897321680617	22.376829808151435
44-45	22.826009872586386	26.690048107812657	27.972818767223185	22.511123252377768
46-47	23.084876078879677	26.823289229974144	27.549960151428298	22.54187453971788
48-49	23.12645391793215	26.829452122702712	27.267792418954418	22.776301540410717
50-51	23.155202682434293	26.930826739756665	27.075122436876896	22.838848140932143
52-53	23.30675518100683	26.94016412717117	26.95357059141606	22.79951010040594
54-55	23.356957634340674	26.899395366163315	26.857534437908313	22.886112561587698
56-57	23.326004671479236	26.981067396838476	26.838701558110667	22.85422637357162
58-59	23.41814952202618	27.062585774382285	26.730245330105923	22.789019373485615
60-61	23.38800883951439	27.018534325602616	26.691955944639016	22.901500890243977
62-63	23.406488768139685	27.10389415121062	26.65631579448522	22.833301286164474
64-65	23.467634561545246	27.167047061220085	26.5717092740617	22.793609103172976
66-67	23.50234948606908	27.170718799315253	26.445259692283372	22.8816720223323
68-69	23.48129373084295	27.293399391148306	26.35621886164231	22.86908801636643
70-71	23.55095862304204	27.340732844742842	26.274510388518607	22.833798143696512
72-73	23.56180612513532	27.37170964267112	26.12364236284097	22.94284186935259
74-75	23.615940088441505	27.467139200459805	26.039164046311797	22.87775666478689
76-77	23.173402318031197	27.835897665244392	25.864276986046995	23.12642303067741
78-79	23.3933309833733	27.76766865951129	25.778650983304725	23.060349373810688
80-81	23.335591685126104	27.87256068284687	25.603545106704235	23.18830252532279
82-83	23.32032416587129	27.67791978938271	25.353157594445847	23.648598450300153
84-85	23.227996062225895	28.095645169790235	25.33392403423422	23.342434733749656
86-87	23.22267305184824	28.159201554905678	25.224105778486468	23.394019614759618
88-89	23.471446617048848	28.145046124270557	25.130531818907407	23.252975439773188
90-91	23.23438997250121	28.211033580997785	25.088201707011525	23.466374739489485
92-93	23.19434602296382	28.347538541267976	24.987517233968486	23.47059820179972
94-95	23.271658506611523	28.387166367931727	24.828812507751604	23.512362617705143
96-97	23.15651711937001	28.454241279084773	24.590570734861544	23.798670866683672
98-99	23.11861213036113	28.48970247025856	24.491947153641817	23.899738245738494
100	23.83917942568638	28.501650703241282	23.828174364737237	23.830995506335103
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1810.0
1	1381.5
2	893.5
3	830.5
4	893.0
5	1031.0
6	1165.0
7	1369.5
8	1680.0
9	2082.0
10	2587.0
11	3247.5
12	4218.0
13	5651.0
14	8631.0
15	12147.0
16	17431.5
17	21417.0
18	22792.0
19	26531.0
20	38090.0
21	47351.0
22	52696.0
23	64071.5
24	77777.0
25	94060.0
26	113489.0
27	138627.5
28	168550.0
29	199297.0
30	229838.0
31	266360.5
32	312177.5
33	363343.5
34	420789.5
35	488644.5
36	564968.0
37	653538.5
38	742426.5
39	813016.0
40	870017.0
41	942412.5
42	1054096.0
43	1155064.0
44	1220005.0
45	1265556.5
46	1258647.5
47	1204142.0
48	1147230.5
49	1110500.0
50	1070390.5
51	998453.5
52	901531.0
53	796263.0
54	709723.5
55	642305.0
56	570436.0
57	502720.5
58	452086.5
59	407366.5
60	361127.5
61	316871.5
62	279267.5
63	247075.0
64	218578.5
65	191040.5
66	164386.5
67	140267.0
68	118639.5
69	99502.5
70	82866.5
71	68864.5
72	57263.5
73	47129.5
74	38473.5
75	30930.0
76	24756.5
77	19684.0
78	15383.5
79	12106.5
80	9453.0
81	7157.0
82	5298.5
83	3960.5
84	2861.0
85	1950.0
86	1373.5
87	973.5
88	671.0
89	456.0
90	317.0
91	214.0
92	140.0
93	90.0
94	56.0
95	31.5
96	17.5
97	8.0
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0735815100341963
2	3.7265895180651456E-6
3	0.0011217034449376088
4	1.6769652831293157E-4
5	0.010251847764197216
6	1.6024334927680127E-4
7	6.968722398781823E-4
8	3.57752593734254E-4
9	7.117785979504428E-4
10-11	2.422283186742345E-5
12-13	1.8073959162615957E-4
14-15	0.0010192222331908173
16-17	8.515257048778858E-4
18-19	8.571155891549835E-5
20-21	8.571155891549835E-5
22-23	0.0015893904294547849
24-25	0.005766897279205813
26-27	0.002625382315476895
28-29	1.7701300210809444E-4
30-31	1.4906358072260583E-5
32-33	0.0
34-35	3.353930566258631E-5
36-37	3.260765828307003E-4
38-39	3.4843611993909114E-4
40-41	2.5154479246939737E-4
42-43	5.888011438542931E-4
44-45	3.130335195174722E-4
46-47	3.763855413245797E-4
48-49	4.658236897581432E-5
50-51	1.9937253921648528E-4
52-53	4.3601097361362204E-4
54-55	3.540260042161889E-4
56-57	0.0018726112328277355
58-59	0.0026421519683081885
60-61	0.0030278539834279307
62-63	0.005006673017520524
64-65	0.005668142656977087
66-67	0.002852704276078869
68-69	0.0026943242215611003
70-71	0.0058116163534225945
72-73	0.005735221268302259
74-75	0.007835154461731969
76-77	0.008010304169081032
78-79	0.009409638533114493
80-81	0.007207224127937993
82-83	0.005375605379808973
84-85	0.010378551807811432
86-87	0.005343929368905419
88-89	7.919002725888435E-4
90-91	3.633424780113517E-4
92-93	7.099153031914103E-4
94-95	0.003857020151197426
96-97	0.005360699021736712
98-99	0.003741495876137406
100	0.004017263500474227
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.6834187E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.94281561910059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.1037309166591	31.568438384655195
2	12.224240883242933	10.009896811321735
3	4.27819346473973	5.254838586290397
4	1.9245967942100362	3.1519364674581443
5	1.0221843545623626	2.092555277878808
6	0.6653487943878938	1.6344751806608646
7	0.43833784469025255	1.256274988781887
8	0.31641711023327185	1.036400592240758
9	0.23352626339145877	0.8605100469828616
>10	1.4717900569107651	11.136028396250106
>50	0.10441550068893805	3.0014612187177097
>100	0.17999079261556014	18.075955925633483
>500	0.03501959688996383	9.115946502736763
>1k	0.002088695116575449	1.1278548447613523
>5k	1.8297178649847097E-5	0.0679805288817378
>10k+	1.0063448257415903E-4	0.6094462467481421
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.7265895180651456E-6	0.0	0.0	0.0
3	0.0	3.7265895180651456E-6	0.0	0.0	0.0
4	0.0	7.453179036130291E-6	0.0	0.0	3.7265895180651456E-6
5	0.0	7.453179036130291E-6	0.0	0.0	3.7265895180651456E-6
6	0.0	7.453179036130291E-6	0.0	0.0	3.7265895180651456E-6
7	0.0	7.453179036130291E-6	0.0	3.7265895180651456E-6	3.7265895180651456E-6
8	0.0	1.4906358072260583E-5	0.0	3.7265895180651456E-6	3.7265895180651456E-6
9	0.0	2.2359537108390876E-5	0.0	3.7265895180651456E-6	3.7265895180651456E-6
10-11	4.844566373484689E-5	2.2359537108390876E-5	0.0	9.316473795162865E-6	3.7265895180651456E-6
12-13	2.608612662645602E-4	2.422283186742345E-5	0.0	1.1179768554195438E-5	3.7265895180651456E-6
14-15	7.639508512033549E-4	2.9812716144521165E-5	0.0	1.1179768554195438E-5	3.7265895180651456E-6
16-17	0.0018446618114422472	2.9812716144521165E-5	0.0	1.1179768554195438E-5	3.7265895180651456E-6
18-19	0.0034228724723428363	2.9812716144521165E-5	0.0	1.1179768554195438E-5	3.7265895180651456E-6
20-21	0.005362562316495744	3.540260042161889E-5	0.0	1.1179768554195438E-5	3.7265895180651456E-6
22-23	0.007851924114563263	3.912918993968403E-5	0.0	1.1179768554195438E-5	3.7265895180651456E-6
24-25	0.011079150637207678	4.471907421678175E-5	0.0	1.863294759032573E-5	3.7265895180651456E-6
26-27	0.014550468773285363	4.471907421678175E-5	0.0	2.422283186742345E-5	7.453179036130291E-6
28-29	0.018135447889664032	4.471907421678175E-5	0.0	2.6086126626456022E-5	9.316473795162865E-6
30-31	0.021085043493212594	4.471907421678175E-5	0.0	2.7949421385488595E-5	2.2359537108390876E-5
32-33	0.02385576279989403	4.658236897581432E-5	0.0	2.9812716144521165E-5	2.6086126626456022E-5
34-35	0.02666561129651515	4.84456637348469E-5	0.0	2.9812716144521165E-5	2.6086126626456022E-5
36-37	0.029835075681629557	4.84456637348469E-5	0.0	3.353930566258631E-5	2.6086126626456022E-5
38-39	0.03251635683987743	5.0308958493879474E-5	0.0	3.912918993968403E-5	2.9812716144521165E-5
40-41	0.03523304059854692	5.4035548011944614E-5	0.0	4.471907421678175E-5	3.540260042161889E-5
42-43	0.03826275787673389	5.962543228904233E-5	0.0	4.658236897581432E-5	3.726589518065146E-5
44-45	0.0415812858425709	5.962543228904233E-5	0.0	4.84456637348469E-5	3.726589518065146E-5
46-47	0.045309738655395074	5.962543228904233E-5	0.0	5.2172253252912044E-5	4.0992484698716605E-5
48-49	0.04968289145484452	5.962543228904233E-5	0.0	5.2172253252912044E-5	5.4035548011944614E-5
50-51	0.05474918990465409	6.521531656614006E-5	0.0	5.7762137530009754E-5	5.5898842770977184E-5
52-53	0.06098004757885901	6.707861132517262E-5	0.0	5.962543228904233E-5	6.707861132517262E-5
54-55	0.06871831071312129	7.080520084323778E-5	0.0	5.962543228904233E-5	6.707861132517262E-5
56-57	0.07836645097539194	7.453179036130292E-5	0.0	6.335202180710747E-5	6.707861132517262E-5
58-59	0.09040333511874238	7.453179036130292E-5	0.0	6.707861132517262E-5	6.707861132517262E-5
60-61	0.10489976834401579	7.825837987936806E-5	0.0	6.707861132517262E-5	6.89419060842052E-5
62-63	0.1225134937011507	8.012167463840063E-5	0.0	7.080520084323778E-5	7.080520084323778E-5
64-65	0.14373828430129074	8.757485367453093E-5	0.0	7.453179036130292E-5	7.453179036130292E-5
66-67	0.1697927349168432	8.94381484335635E-5	1.8632947590325728E-6	7.453179036130292E-5	7.453179036130292E-5
68-69	0.2019923316476851	9.130144319259608E-5	3.7265895180651456E-6	7.825837987936806E-5	7.453179036130292E-5
70-71	0.2403892467470693	9.502803271066122E-5	9.316473795162865E-6	7.825837987936806E-5	7.825837987936806E-5
72-73	0.28475615825439393	9.68913274696938E-5	1.1179768554195438E-5	7.825837987936806E-5	7.825837987936806E-5
74-75	0.33590918927411517	9.875462222872636E-5	1.1179768554195438E-5	8.571155891549835E-5	7.825837987936806E-5
76-77	0.39635447125713186	1.0061791698775893E-4	1.304306331322801E-5	9.316473795162864E-5	7.825837987936806E-5
78-79	0.4681155423117533	1.0061791698775893E-4	1.4906358072260583E-5	1.0434450650582409E-4	7.825837987936806E-5
80-81	0.5498564200957532	1.0061791698775893E-4	1.4906358072260583E-5	1.0620780126485666E-4	7.825837987936806E-5
82-83	0.6430696037111168	1.0248121174679151E-4	1.4906358072260583E-5	1.0993439078292179E-4	7.825837987936806E-5
84-85	0.7494562812728405	1.0434450650582409E-4	1.4906358072260583E-5	1.1925086457808466E-4	8.198496939743321E-5
86-87	0.8718766102360396	1.0434450650582409E-4	1.4906358072260583E-5	1.2670404361421497E-4	8.571155891549835E-5
88	0.9717529359097036	1.0807109602388923E-4	1.4906358072260583E-5	1.2670404361421497E-4	8.571155891549835E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	17090	0.0	44.83372	1
TCTCGAG	20590	0.0	37.026768	2
CTCGAGG	25955	0.0	29.282646	3
GATCCGG	25610	0.0	27.677664	1
TCGAGGG	30520	0.0	24.841026	4
TATCTCG	10005	0.0	23.771399	2
ATCCGGC	31135	0.0	22.750225	2
TCCGGCG	40090	0.0	17.727074	3
GGATCCG	16670	0.0	17.650625	2
GCGCCGT	40905	0.0	17.39517	9
CGAGGGC	45035	0.0	17.095596	5
ATATCTC	15080	0.0	16.281517	1
AGGGCGC	47120	0.0	15.988223	7
CGGCGCG	44400	0.0	15.910965	5
ATGGATC	18235	0.0	15.321681	1
CGTCGAC	39040	0.0	14.993821	12-13
CCGGCGC	49090	0.0	14.534444	4
TGGATCC	21075	0.0	14.439841	1
GCCGTCG	41615	0.0	14.212869	10-11
GGGCGCG	53255	0.0	14.199321	8
>>END_MODULE