FastQCFastQC Report
Wed 25 May 2016
SRR491017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53804158
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT556920.10350872882352326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA306350.043.956141
TCTCGAG366950.036.377562
GATCCGG492200.028.8685651
CTCGAGG466600.028.5481013
TCGAGGG549050.024.1668424
ATCCGGC602300.023.6066672
TCCGGCG758900.018.8964313
TATCTCG205150.018.4894562
GCGCCGT786250.018.0519969
CGGCGCG831400.017.0507165
GGATCCG327350.016.052832
CGAGGGC841350.016.0334325
AGGGCGC873800.015.1566597
CGTCGAC752750.015.09986412-13
CCGGCGC951750.015.0970974
TCGACTT790400.014.44601614-15
ATGGATC343850.014.4174241
GCCGTCG803100.014.314116510-11
ATATCTC288900.013.6268031
GGGCGCG979000.013.5567548
Produced by FastQC (version 0.11.5)