##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491017_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53804158
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.222747245668263	33.0	31.0	34.0	28.0	34.0
2	31.507972636612955	34.0	31.0	34.0	28.0	34.0
3	31.56796716343001	34.0	31.0	34.0	28.0	34.0
4	34.96625288699806	37.0	35.0	37.0	32.0	37.0
5	34.677525220262716	37.0	35.0	37.0	32.0	37.0
6	34.62290674635221	37.0	35.0	37.0	31.0	37.0
7	34.542705528446334	37.0	35.0	37.0	31.0	37.0
8	34.44309395195814	37.0	35.0	37.0	31.0	37.0
9	35.920506627015705	39.0	37.0	39.0	30.0	39.0
10-11	35.77836171137554	39.0	37.0	39.0	30.0	39.0
12-13	35.56048865219673	39.0	36.0	39.0	30.0	39.0
14-15	36.82217509100319	40.0	37.0	41.0	30.0	41.0
16-17	36.73921242295066	40.0	37.0	41.0	30.0	41.0
18-19	36.62241394057314	40.0	37.0	41.0	30.0	41.0
20-21	36.481196666993654	40.0	37.0	41.0	29.0	41.0
22-23	36.29846824291907	40.0	37.0	41.0	28.5	41.0
24-25	36.07852973928149	40.0	36.0	41.0	27.0	41.0
26-27	35.74406704775494	40.0	36.0	41.0	27.0	41.0
28-29	35.42939003896316	39.0	35.0	41.0	25.5	41.0
30-31	35.16464210814339	39.0	35.0	41.0	24.5	41.0
32-33	34.92278733364808	39.0	35.0	41.0	24.0	41.0
34-35	34.65522376541976	39.0	35.0	40.0	22.5	41.0
36-37	34.387723101251765	39.0	34.5	40.0	20.0	41.0
38-39	34.039168794352285	38.0	34.0	40.0	17.0	41.0
40-41	33.62533066682319	38.0	33.0	40.0	12.0	41.0
42-43	33.17996166764658	38.0	33.0	40.0	7.5	41.0
44-45	32.71606826372044	38.0	32.5	40.0	2.0	41.0
46-47	32.211372502102904	38.0	31.5	40.0	2.0	41.0
48-49	31.795391910045318	37.5	31.0	40.0	2.0	41.0
50-51	31.297926518987623	37.0	30.5	40.0	2.0	41.0
52-53	30.774600347802114	37.0	29.5	39.5	2.0	41.0
54-55	30.264986425398572	36.0	28.5	39.0	2.0	40.0
56-57	29.66738984187802	35.5	27.5	39.0	2.0	40.0
58-59	29.070398453219916	35.0	26.0	38.5	2.0	40.0
60-61	28.487402562827953	34.5	25.5	38.0	2.0	40.0
62-63	27.88117126561111	34.0	23.5	38.0	2.0	40.0
64-65	27.234358262794487	34.0	22.0	37.5	2.0	39.5
66-67	26.5801493074197	33.0	19.5	37.0	2.0	39.0
68-69	25.935401479565947	32.5	16.0	36.0	2.0	39.0
70-71	25.16746720578733	32.0	8.5	35.5	2.0	38.5
72-73	24.45078590394445	31.0	4.5	35.0	2.0	38.0
74-75	23.74820617023688	30.5	2.0	35.0	2.0	37.0
76-77	22.76619779088449	29.5	2.0	34.0	2.0	36.0
78-79	22.806384396908506	30.0	2.0	34.0	2.0	36.0
80-81	22.2316862667008	29.5	2.0	34.0	2.0	35.5
82-83	21.35237206945976	29.0	2.0	34.0	2.0	35.0
84-85	20.51616600895418	27.5	2.0	33.5	2.0	35.0
86-87	19.9117131895271	26.5	2.0	33.0	2.0	35.0
88-89	19.254622337924143	26.0	2.0	32.5	2.0	34.5
90-91	18.484044383707296	25.0	2.0	32.0	2.0	34.0
92-93	17.763507515534393	23.5	2.0	32.0	2.0	34.0
94-95	16.891967801075893	19.0	2.0	32.0	2.0	34.0
96-97	15.801825167489843	6.0	2.0	31.0	2.0	34.0
98-99	14.746300880686581	2.0	2.0	31.0	2.0	34.0
100	13.948288308870106	2.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.2841386330004134
1101	2	0.29245239130689527
1101	3	0.2686716220652805
1101	4	0.2812648106406499
1101	5	0.34521035251276544
1101	6	0.3084603111802551
1101	7	0.3257289188740202
1101	8	0.3265873459360904
1101	9	0.3999944755125
1101	10-11	0.37665350751795756
1101	12-13	0.3826887582347922
1101	14-15	0.4353882697387874
1101	16-17	0.4327386152742321
1101	18-19	0.3878623674543533
1101	20-21	0.4070288032232483
1101	22-23	0.40091945065255175
1101	24-25	0.42050841299245434
1101	26-27	0.3923166217187344
1101	28-29	0.3949784523104043
1101	30-31	0.4133986236102061
1101	32-33	0.3146527850341272
1101	34-35	0.29537002209070096
1101	36-37	0.30156191314556935
1101	38-39	0.27881234671074395
1101	40-41	0.1865555138792132
1101	42-43	0.20792898585781217
1101	44-45	0.17449754678008844
1101	46-47	0.14367962538376133
1101	48-49	0.09912704824850138
1101	50-51	0.055004099414901475
1101	52-53	0.01106931635591124
1101	54-55	0.031285555175585955
1101	56-57	0.04927102931225846
1101	58-59	-0.03521134458911135
1101	60-61	-0.08408176633353293
1101	62-63	-0.08464283517887594
1101	64-65	-0.14955832036250527
1101	66-67	-0.32900977760494143
1101	68-69	-0.3697877114683017
1101	70-71	-0.2925328129611877
1101	72-73	-0.2928460649131601
1101	74-75	-0.35377611557040467
1101	76-77	-0.23825004398559813
1101	78-79	-0.19227117011661576
1101	80-81	-0.26210000226618035
1101	82-83	-0.09181966680814924
1101	84-85	0.11865729266911629
1101	86-87	-0.2432110946898014
1101	88-89	-0.5211240241051911
1101	90-91	-0.643343163044495
1101	92-93	-0.668119165973792
1101	94-95	-0.4303859356378368
1101	96-97	-0.15581677125738302
1101	98-99	-0.05801829866638997
1101	100	0.06103803502505656
1102	1	0.20398034952805588
1102	2	0.19822481821729454
1102	3	0.2150900954479802
1102	4	0.23179965839985073
1102	5	0.23891198787014645
1102	6	0.2486245822794828
1102	7	0.2459972570567288
1102	8	0.2330093576880401
1102	9	0.2559714903973571
1102	10-11	0.2611772357818296
1102	12-13	0.2638586916823016
1102	14-15	0.31704406661039997
1102	16-17	0.3172260416658901
1102	18-19	0.2711125189732684
1102	20-21	0.2823989788955217
1102	22-23	0.27772902727461
1102	24-25	0.27343090274839454
1102	26-27	0.28717871372633397
1102	28-29	0.28378141800128276
1102	30-31	0.25172215471118164
1102	32-33	0.25763187532269427
1102	34-35	0.20561772161736513
1102	36-37	0.25336569420711186
1102	38-39	0.20399526653177702
1102	40-41	0.19957163979560022
1102	42-43	0.25860300220761445
1102	44-45	0.1631057414353947
1102	46-47	0.11333948351123269
1102	48-49	0.06845407601502629
1102	50-51	0.04863447137053356
1102	52-53	0.03362527516985736
1102	54-55	0.042743992185080515
1102	56-57	0.03372900080050556
1102	58-59	-0.05950623471762029
1102	60-61	-0.011562601321745802
1102	62-63	-0.19046188602473535
1102	64-65	-0.17376237261557392
1102	66-67	-0.3054881254698891
1102	68-69	-0.34858275917727966
1102	70-71	-0.17494024605359826
1102	72-73	-0.16047633253480598
1102	74-75	-0.28224301334629587
1102	76-77	-0.14429963409510904
1102	78-79	-0.0055162178128078665
1102	80-81	-0.10675645392771571
1102	82-83	0.08482763563728923
1102	84-85	0.29732003224262726
1102	86-87	-0.08860800147055414
1102	88-89	-0.2231719102186709
1102	90-91	-0.3947169559185788
1102	92-93	-0.5210644271933802
1102	94-95	-0.30174276840705616
1102	96-97	0.012282548815459648
1102	98-99	0.08543596879376203
1102	100	0.11311455611979859
1103	1	0.029221559852448564
1103	2	0.03473342748260322
1103	3	-0.007153364239016469
1103	4	0.03066113847575025
1103	5	0.11247773188376442
1103	6	-0.0026662797728960186
1103	7	0.05421874382520997
1103	8	0.05139132557149395
1103	9	0.0411754380795486
1103	10-11	0.03990367200805878
1103	12-13	0.03521449362268214
1103	14-15	-0.017818461917102013
1103	16-17	0.08278706491086751
1103	18-19	0.06336235459075823
1103	20-21	0.07434395043251385
1103	22-23	0.07155785758260436
1103	24-25	0.07070895117418985
1103	26-27	0.07900805066865502
1103	28-29	0.11448944729537658
1103	30-31	0.012316117006015759
1103	32-33	-0.15572034343074392
1103	34-35	0.04121505365596789
1103	36-37	-0.061453289568866865
1103	38-39	-0.02662915246106934
1103	40-41	-0.05118553615584176
1103	42-43	-0.005360520120653689
1103	44-45	-0.026571767831406135
1103	46-47	-0.040661692380794534
1103	48-49	-0.09626295542053853
1103	50-51	-0.11124543361989225
1103	52-53	-0.11786186468389204
1103	54-55	-0.3125542887764894
1103	56-57	-0.16839652958231
1103	58-59	-0.4012353315559274
1103	60-61	-0.41705796929229066
1103	62-63	-0.35226790058734636
1103	64-65	-0.3159737415524795
1103	66-67	-0.4244694347287101
1103	68-69	-0.29996636736755633
1103	70-71	-0.17464096824206976
1103	72-73	-0.19362333311102375
1103	74-75	-0.3151510044864487
1103	76-77	-0.16956187599892658
1103	78-79	-0.13449454043098186
1103	80-81	-0.19334905659739832
1103	82-83	0.0514007977782569
1103	84-85	0.32198466846276474
1103	86-87	-0.0035834555063871676
1103	88-89	-0.17004808738118982
1103	90-91	-0.5263080535402871
1103	92-93	-0.8055850361866135
1103	94-95	-0.5555309311461372
1103	96-97	-0.32047148178118867
1103	98-99	-0.5141780196302683
1103	100	-0.42712091156701604
1104	1	-0.002325941216675176
1104	2	0.02915066858565396
1104	3	0.07483821932139989
1104	4	0.036038271371488406
1104	5	0.09202033336996607
1104	6	0.050849230192348216
1104	7	0.05437757763429829
1104	8	-0.0071327401211291885
1104	9	-0.018257544464390207
1104	10-11	-0.009535221807269068
1104	12-13	0.039616955175411306
1104	14-15	0.10931182640039339
1104	16-17	0.07148909201922038
1104	18-19	0.09531493962764159
1104	20-21	0.08021946450781314
1104	22-23	0.07943736079436547
1104	24-25	0.12922810818400166
1104	26-27	0.1224900654871135
1104	28-29	0.11998489341688412
1104	30-31	0.1352405743689573
1104	32-33	0.1784835180145805
1104	34-35	0.18096668994454745
1104	36-37	0.12839061413470887
1104	38-39	0.19035354727286347
1104	40-41	0.05594222385504111
1104	42-43	0.12041254769339105
1104	44-45	0.12946854848448908
1104	46-47	0.14136068996904072
1104	48-49	0.08867443755284299
1104	50-51	0.12688637803777425
1104	52-53	0.1759187198693759
1104	54-55	0.06340676222350794
1104	56-57	0.25948941969095785
1104	58-59	0.23297133645148804
1104	60-61	0.2103207986573601
1104	62-63	0.23466775316351018
1104	64-65	0.1469749320399849
1104	66-67	0.07020510672819924
1104	68-69	0.107143397493072
1104	70-71	0.1639791024517443
1104	72-73	0.22032305695734777
1104	74-75	0.197778481824205
1104	76-77	0.23756945171826516
1104	78-79	0.310368642467882
1104	80-81	0.18794319730952225
1104	82-83	0.44010451271075013
1104	84-85	0.7070861574062555
1104	86-87	0.4027990345733592
1104	88-89	0.2785720464173984
1104	90-91	-0.01116345168753341
1104	92-93	-0.12330327971361399
1104	94-95	0.10522279093823173
1104	96-97	0.3304956887816939
1104	98-99	0.32220784221087584
1104	100	0.23140656910005752
1105	1	-0.035752701595107794
1105	2	-0.015171883717204793
1105	3	-0.0012900827816721971
1105	4	-0.027346652483146272
1105	5	-0.016803763498764113
1105	6	-0.04910948216479483
1105	7	-0.03551491994094391
1105	8	-0.036020301142038136
1105	9	-0.03669864715023863
1105	10-11	-0.042593051493113876
1105	12-13	-0.040100789868446896
1105	14-15	-0.03284957764766716
1105	16-17	-0.032505120668474774
1105	18-19	-0.01779435633653037
1105	20-21	-0.053070979567579
1105	22-23	-0.0440208184779749
1105	24-25	-0.03250846541686769
1105	26-27	-0.039295687151764014
1105	28-29	-0.0031722340261666204
1105	30-31	-9.079606441630972E-4
1105	32-33	0.030126331633617554
1105	34-35	-0.07115266976155965
1105	36-37	-0.0054077152207625545
1105	38-39	9.323379212844429E-4
1105	40-41	-0.0034296454817663857
1105	42-43	-0.0065918600042991216
1105	44-45	-0.027419141343635545
1105	46-47	-0.010160297717668243
1105	48-49	-0.0052377172069135725
1105	50-51	-0.05480387261873432
1105	52-53	-0.0013975219791326765
1105	54-55	-0.09955492224179352
1105	56-57	0.0761521669628884
1105	58-59	0.10945313506841003
1105	60-61	-0.12059736310335367
1105	62-63	-0.06040561410408074
1105	64-65	0.08201280776999553
1105	66-67	0.07188107710788927
1105	68-69	0.013532864434484537
1105	70-71	0.13020555999417738
1105	72-73	0.17232015738287743
1105	74-75	0.1970470644976814
1105	76-77	0.2543189003883981
1105	78-79	0.33518556370691854
1105	80-81	0.21648193460644194
1105	82-83	0.5973944539981026
1105	84-85	0.9851563729755988
1105	86-87	0.5936345653329482
1105	88-89	0.4864454901581041
1105	90-91	0.34746377583289956
1105	92-93	0.18950892255217866
1105	94-95	0.056112320781398495
1105	96-97	0.3404650972583685
1105	98-99	0.46224127498975776
1105	100	0.36095600609996836
1106	1	-0.3138476207630845
1106	2	-0.3783288736434116
1106	3	-0.3224130907192233
1106	4	-0.2943455107204471
1106	5	-0.28963286995809057
1106	6	-0.40589612891905347
1106	7	-0.381848239457085
1106	8	-0.4289291652664744
1106	9	-0.434569989153502
1106	10-11	-0.4436635076516282
1106	12-13	-0.4928706804451508
1106	14-15	-0.5108707547048255
1106	16-17	-0.4708971969190685
1106	18-19	-0.48137204979911985
1106	20-21	-0.5318999573670808
1106	22-23	-0.564093275632203
1106	24-25	-0.582471967131724
1106	26-27	-0.5274139534225242
1106	28-29	-0.5564724541921748
1106	30-31	-0.591219487974115
1106	32-33	-0.523934671225895
1106	34-35	-0.6715043930572122
1106	36-37	-0.6631188665294161
1106	38-39	-0.6464023470389151
1106	40-41	-0.6549534844843876
1106	42-43	-0.6072867834658311
1106	44-45	-0.6554984232195622
1106	46-47	-0.7239286193794001
1106	48-49	-0.6967371988713964
1106	50-51	-0.7084294732795868
1106	52-53	-0.6101692772898808
1106	54-55	-0.7486121346479564
1106	56-57	-0.6277011151493852
1106	58-59	-0.684205213879391
1106	60-61	-0.7639880184502879
1106	62-63	-0.7009882238387739
1106	64-65	-0.6237675956183644
1106	66-67	-0.6255967894046215
1106	68-69	-0.632395766972774
1106	70-71	-0.4462253431502141
1106	72-73	-0.5262793518101709
1106	74-75	-0.6109019817884871
1106	76-77	-0.4753909061969104
1106	78-79	-0.4416053466058223
1106	80-81	-0.4737431301286321
1106	82-83	-0.133421589964847
1106	84-85	0.38457851550260713
1106	86-87	0.09138031893579424
1106	88-89	0.08571266063830763
1106	90-91	-0.027788584375599612
1106	92-93	-0.1726799724301138
1106	94-95	-0.21147669933053592
1106	96-97	0.04934995924701546
1106	98-99	0.08797819661867479
1106	100	0.0017339021453679493
1107	1	-0.36961211115643877
1107	2	-0.3534652192544563
1107	3	-0.35844057255558326
1107	4	-0.3167634169888842
1107	5	-0.4090716069971947
1107	6	-0.41059615550668127
1107	7	-0.49557984018994006
1107	8	-0.47823264588634373
1107	9	-0.5114005934493449
1107	10-11	-0.46003888144210237
1107	12-13	-0.46909320834913615
1107	14-15	-0.5232360075768696
1107	16-17	-0.5837382647836904
1107	18-19	-0.5672638306541558
1107	20-21	-0.5184687818053746
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2207	90-91	1.1499248798793502
2207	92-93	0.9341768001288742
2207	94-95	0.782220307888462
2207	96-97	0.6827087450730982
2207	98-99	0.6272535879378136
2207	100	0.4551416189557944
2208	1	-0.58261292313707
2208	2	-0.5422671892146411
2208	3	-0.9189228873217097
2208	4	-0.6746661126351086
2208	5	-1.2123422459095181
2208	6	-0.8276091873230271
2208	7	-0.7048486798886273
2208	8	-0.7356198204742483
2208	9	-0.6756978746791518
2208	10-11	-0.6497733004518977
2208	12-13	-0.6173827851961704
2208	14-15	-0.7712802706695925
2208	16-17	-0.7070548492047237
2208	18-19	-0.7421785901050413
2208	20-21	-0.8372853029697538
2208	22-23	-0.795212536257516
2208	24-25	-0.7918373336814355
2208	26-27	-0.832404751203299
2208	28-29	-0.8421380233847344
2208	30-31	-0.8596459228756501
2208	32-33	-0.8590581775110522
2208	34-35	-0.9124710708026669
2208	36-37	-0.822796058870928
2208	38-39	-0.8368836978034295
2208	40-41	-0.8467278623897414
2208	42-43	-0.8938262193114426
2208	44-45	-0.8940265119357598
2208	46-47	-0.7038863680283143
2208	48-49	-0.7740562999804652
2208	50-51	-0.7600200508892883
2208	52-53	-0.5948816320130952
2208	54-55	-0.5569379317713867
2208	56-57	-0.7368295969864107
2208	58-59	-0.678507869000498
2208	60-61	-0.5729234817513138
2208	62-63	-0.5840321469649332
2208	64-65	-0.7524894559093873
2208	66-67	-0.3169437773731971
2208	68-69	-0.3798054920130518
2208	70-71	-0.25710311825594445
2208	72-73	-0.24998893205872008
2208	74-75	-0.30419587598815667
2208	76-77	-0.39045526025521227
2208	78-79	-0.5127971292620792
2208	80-81	-0.5004315999915505
2208	82-83	-0.40215850376703344
2208	84-85	-0.29304654855622303
2208	86-87	-0.607699285423223
2208	88-89	-1.2491364701353405
2208	90-91	-2.433001153930743
2208	92-93	-2.601282084457889
2208	94-95	-2.7321732139991397
2208	96-97	-3.5840385295580752
2208	98-99	-3.5436125278356947
2208	100	-3.4148241819017944
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1046894.0
3	404809.0
4	341880.0
5	272651.0
6	252300.0
7	260391.0
8	277597.0
9	312364.0
10	357414.0
11	402308.0
12	447149.0
13	514910.0
14	615820.0
15	751651.0
16	867948.0
17	876780.0
18	834859.0
19	808459.0
20	828625.0
21	862706.0
22	908017.0
23	962123.0
24	1024413.0
25	1094537.0
26	1187415.0
27	1305205.0
28	1460072.0
29	1656095.0
30	1901604.0
31	2188879.0
32	2573902.0
33	3131430.0
34	3992782.0
35	5324424.0
36	6565726.0
37	5473564.0
38	1658633.0
39	57785.0
40	37.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.510263866891975	28.28645828005249	28.039362880448483	22.163914972607056
2	23.44696055536975	28.15981962731317	27.173286152342698	21.219933664974384
3	23.08875572215832	26.757009136309602	27.162692313940106	22.991542827591974
4	24.070674944230525	26.34841505964	26.7954109423868	22.78549905374268
5	24.26951920109645	26.576350858745922	26.5753954388253	22.578734501332328
6	24.21770317507231	26.4489118721994	26.06057505022018	23.27280990250811
7	24.21542151243213	26.722028717582447	26.22802237828607	22.83452739169935
8	23.62511931738579	26.63393959457793	26.56391689105218	23.177024196984096
9	23.572927749767963	26.330419150275713	26.698203868956515	23.398449230999812
10-11	23.50046013189543	26.556134028204298	26.64827842795288	23.295127411947387
12-13	23.261723533707745	26.595637554516816	26.78041363509455	23.36222527668089
14-15	22.97908611457043	26.831028217918774	27.20924203519951	22.980643632311285
16-17	22.847373974531482	26.733521005275506	27.36573661277573	23.05336840741728
18-19	22.526752122926258	26.689477136354373	27.9320229058032	22.851747834916168
20-21	22.275954264176693	26.440950829681725	28.626840811427734	22.65625409471385
22-23	22.321480256044083	26.402163874163627	28.916576857297088	22.359779012495206
24-25	22.240509485379953	26.292886436681286	29.260147891426836	22.20645618651193
26-27	22.275694135464448	26.332502585070056	29.279162297810686	22.112640981654817
28-29	22.33931507063308	26.384251370855775	29.242455963859314	22.033977594651823
30-31	22.3124935530066	26.31288850395369	29.21779825533904	22.156819687700672
32-33	22.330920037815666	26.400479122821697	29.10802730153309	22.16057353782955
34-35	22.426792182902872	26.42100887345261	29.13140989341871	22.020789050225808
36-37	22.43082732968257	26.385786179069942	29.01960437011015	22.163782121137334
38-39	22.446099410112662	26.46914022363726	28.91136092606344	22.173399440186632
40-41	22.494973028639972	26.589190604385454	28.71818872481388	22.197647642160693
42-43	22.62010051689571	26.628209410137444	28.36966806675131	22.382022006215536
44-45	22.64712876420539	26.843068540171682	28.015528733490203	22.494273962132723
46-47	22.89096761685773	26.958290665308205	27.638056729328298	22.51268498850577
48-49	22.925567587036586	26.95763468107196	27.38161609796215	22.735181633929308
50-51	22.951508723813703	27.038039460561787	27.20996332450742	22.80048849111709
52-53	23.096287403376255	27.054864186229654	27.09194418928445	22.756904221109636
54-55	23.14556194400982	27.00156371250261	27.01951683599482	22.83335750749275
56-57	23.11843052819402	27.07600206545251	26.993824830788014	22.811742575565454
58-59	23.197145695841883	27.150010393043466	26.906222965227535	22.746620945887116
60-61	23.168482304483778	27.102664774181612	26.887038683882842	22.84181423745177
62-63	23.194347648494073	27.179504560532834	26.837273153620238	22.788874637352848
64-65	23.25631240702186	27.24159714579321	26.7515224426553	22.75056800452963
66-67	23.28772882535761	27.232529127193843	26.64547199972016	22.83427004772838
68-69	23.265668291799845	27.337486527180666	26.57261605675693	22.82422912426256
70-71	23.344931113812102	27.383377809536647	26.49557210504943	22.776118971601818
72-73	23.343641582440615	27.402557650707283	26.353998331042433	22.89980243580967
74-75	23.38237288831828	27.49123087701546	26.2848532705059	22.84154296416036
76-77	22.96320571976806	27.861729050251995	26.103578361528555	23.071486868451395
78-79	23.19287471266106	27.78495664637989	26.01845186756569	23.003716773393368
80-81	23.13944395042388	27.876903603883424	25.862356986188068	23.12129545950463
82-83	23.138025884089323	27.673988088047313	25.626506387883396	23.56147963997997
84-85	23.030930865801487	28.07781215424592	25.609036482336613	23.28222049761598
86-87	23.015699147723378	28.15772426366464	25.499602589984153	23.326973998627825
88-89	23.258947217228354	28.125175173604944	25.418496711977497	23.197380897189205
90-91	23.013529772611673	28.200189645851705	25.40005991936859	23.386220662168032
92-93	22.982332997957272	28.32189830972937	25.3090533294403	23.386715362873062
94-95	23.049184866362246	28.37500900871514	25.16853371852299	23.407272406399624
96-97	22.941264529029098	28.4247262359671	24.932078148710858	23.701931086292937
98-99	22.88841126189582	28.472329405159236	24.851924913854656	23.78733441909029
100	23.595820088293532	28.480718023686226	24.215584423019095	23.707877465001147
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4975.0
1	3641.0
2	1993.5
3	1687.0
4	1664.5
5	1801.5
6	2147.5
7	2566.0
8	3167.0
9	3887.0
10	4858.0
11	6188.0
12	7842.5
13	10265.5
14	15800.0
15	22597.0
16	32880.5
17	40580.5
18	43283.0
19	50773.5
20	76508.0
21	95474.0
22	103596.5
23	126795.0
24	154909.0
25	188027.5
26	227362.0
27	277661.5
28	337844.0
29	399125.5
30	463408.5
31	541629.0
32	637452.5
33	742274.0
34	861361.5
35	1003232.0
36	1164993.0
37	1353415.5
38	1537274.0
39	1676171.0
40	1782840.0
41	1927748.0
42	2158939.5
43	2363937.0
44	2484677.0
45	2575200.0
46	2564830.0
47	2449352.0
48	2328631.5
49	2249082.5
50	2162968.5
51	2005484.5
52	1796010.5
53	1575025.0
54	1395681.0
55	1259045.5
56	1113759.5
57	976325.5
58	874681.0
59	785126.0
60	691345.0
61	598795.5
62	521918.5
63	459913.0
64	403572.0
65	350057.0
66	300728.5
67	255343.5
68	214221.5
69	178489.5
70	147786.5
71	121913.0
72	100754.0
73	83511.0
74	68877.5
75	55637.0
76	44338.5
77	35208.5
78	28082.0
79	22246.5
80	17181.5
81	13019.5
82	9751.5
83	7138.5
84	5070.5
85	3510.0
86	2424.5
87	1732.5
88	1249.5
89	861.5
90	549.0
91	360.0
92	251.5
93	155.0
94	91.0
95	53.0
96	27.5
97	15.0
98	6.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07491614309808546
2	5.5757772475502735E-6
3	0.0011077210798466541
4	1.5426317051555756E-4
5	0.01082444222991093
6	1.7099050225820837E-4
7	7.34144004260786E-4
8	3.3454663485301637E-4
9	7.434369663400364E-4
10-11	3.717184831700182E-5
12-13	2.1559672023861056E-4
14-15	0.0011105089684704294
16-17	9.655387600341222E-4
18-19	1.1802061840648077E-4
20-21	5.947495730720291E-5
22-23	0.001569581295185402
24-25	0.005953071507967841
26-27	0.0027088984461015077
28-29	1.570510591393327E-4
30-31	1.4868739326800728E-5
32-33	0.0
34-35	3.06667748615265E-5
36-37	2.9644549032808954E-4
38-39	2.8808182445676407E-4
40-41	2.6299082684278787E-4
42-43	4.981027674478244E-4
44-45	2.9086971308053925E-4
46-47	3.847286300809688E-4
48-49	5.761636489135282E-5
50-51	2.025865733276599E-4
52-53	4.181832935662705E-4
54-55	2.787888623775136E-4
56-57	0.0017396425012356851
58-59	0.0024951603182787473
60-61	0.002846434284874414
62-63	0.004739410660417732
64-65	0.005335089529697686
66-67	0.002736777332339259
68-69	0.0025797262731999263
70-71	0.00541872618841094
72-73	0.005408503930123765
74-75	0.007212267869706278
76-77	0.007312631860162183
78-79	0.0086824144706437
80-81	0.006582205040733097
82-83	0.004928057790626516
84-85	0.009445366657350162
86-87	0.004963371046527668
88-89	7.248510421815355E-4
90-91	3.4662748555604195E-4
92-93	7.016186369834094E-4
94-95	0.0038853874453346157
96-97	0.005555332730975922
98-99	0.0038324175614828874
100	0.004192984490157805
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5.3804158E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.77045332649279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.12140173621331	25.424642862367342
2	13.26054030918061	9.221499958088813
3	5.25305077865638	5.479528707629049
4	2.609655183929726	3.6295557508427403
5	1.4750380568417363	2.5643870955108103
6	0.8961311109736421	1.8695330981116292
7	0.6099115783969395	1.4844831448956834
8	0.4358458775985608	1.2123646995668038
9	0.3255918453260262	1.018887845525573
>10	1.6930894555065368	10.928125193460424
>50	0.12402244918905989	2.9501166938801098
>100	0.1266952307279505	10.36839909553106
>500	0.043507013564893005	11.04912092886942
>1k	0.025379539083124243	11.947392583474567
>5k	6.45392351461504E-5	0.15787688525280058
>10k+	7.529557627912256E-5	0.6940854569931215
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55692	0.10350872882352326	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.858592415850091E-6
2	0.0	0.0	0.0	0.0	1.858592415850091E-6
3	0.0	1.858592415850091E-6	0.0	0.0	1.858592415850091E-6
4	0.0	3.717184831700182E-6	0.0	0.0	1.858592415850091E-6
5	1.858592415850091E-6	5.575777247550273E-6	1.858592415850091E-6	0.0	3.717184831700182E-6
6	1.858592415850091E-6	7.434369663400364E-6	1.858592415850091E-6	0.0	3.717184831700182E-6
7	1.858592415850091E-6	7.434369663400364E-6	1.858592415850091E-6	0.0	3.717184831700182E-6
8	1.858592415850091E-6	9.292962079250456E-6	1.858592415850091E-6	0.0	3.717184831700182E-6
9	1.3010146910950636E-5	9.292962079250456E-6	1.858592415850091E-6	0.0	7.434369663400364E-6
10-11	4.925269902002741E-5	9.292962079250456E-6	1.858592415850091E-6	0.0	1.208085070302559E-5
12-13	2.3325334818918644E-4	9.292962079250456E-6	1.858592415850091E-6	0.0	1.672733174265082E-5
14-15	6.635174924584824E-4	1.1151554495100545E-5	1.858592415850091E-6	0.0	1.858592415850091E-5
16-17	0.0015482074824031257	1.3010146910950636E-5	1.858592415850091E-6	9.292962079250455E-7	2.1373812782276047E-5
18-19	0.0029235658701321932	1.3010146910950636E-5	1.858592415850091E-6	1.858592415850091E-6	2.230310899020109E-5
20-21	0.004628824411674651	1.5798035534725773E-5	1.858592415850091E-6	1.858592415850091E-6	2.6949590029826317E-5
22-23	0.006787579502684532	1.672733174265082E-5	1.858592415850091E-6	1.858592415850091E-6	2.9737478653601455E-5
24-25	0.009528074019855491	2.0444516574351E-5	1.858592415850091E-6	2.7878886237751363E-6	3.1596071069451546E-5
26-27	0.012863318110098481	2.3232405198126134E-5	1.858592415850091E-6	3.717184831700182E-6	3.438395969322669E-5
28-29	0.01590583389484508	2.6020293821901273E-5	1.858592415850091E-6	3.717184831700182E-6	3.6242552109076776E-5
30-31	0.018466974243886505	2.6020293821901273E-5	1.858592415850091E-6	3.717184831700182E-6	3.995973694077696E-5
32-33	0.020950053711462226	3.1596071069451546E-5	1.858592415850091E-6	3.717184831700182E-6	4.274762556455209E-5
34-35	0.02353814365053348	3.345466348530164E-5	1.858592415850091E-6	3.717184831700182E-6	4.553551418832723E-5
36-37	0.026195930805199108	3.438395969322669E-5	2.7878886237751363E-6	3.717184831700182E-6	4.925269902002741E-5
38-39	0.028551696692289096	4.1818329356627046E-5	3.717184831700182E-6	3.717184831700182E-6	5.018199522795246E-5
40-41	0.03086285636139869	4.460621798040218E-5	3.717184831700182E-6	3.717184831700182E-6	5.018199522795246E-5
42-43	0.03350577477673752	4.460621798040218E-5	3.717184831700182E-6	4.646481039625227E-6	5.11112914358775E-5
44-45	0.03647487616105804	4.553551418832723E-5	5.575777247550273E-6	5.575777247550273E-6	5.389918005965264E-5
46-47	0.03993092875833128	4.832340281210236E-5	6.505073455475318E-6	6.505073455475318E-6	5.6687068683427775E-5
48-49	0.043899952862379146	4.832340281210236E-5	7.434369663400364E-6	7.434369663400364E-6	5.854566109927786E-5
50-51	0.0484693023167466	5.018199522795246E-5	8.36366587132541E-6	7.434369663400364E-6	6.1333549723053E-5
52-53	0.05380160395782051	5.018199522795246E-5	9.292962079250456E-6	7.434369663400364E-6	6.226284593097804E-5
54-55	0.0601979497569686	5.11112914358775E-5	9.292962079250456E-6	9.292962079250456E-6	6.505073455475319E-5
56-57	0.06795199731589519	5.482847626757769E-5	9.292962079250456E-6	1.02222582871755E-5	7.248510421815355E-5
58-59	0.07720964613924448	5.854566109927786E-5	9.292962079250456E-6	1.7656627950575864E-5	7.248510421815355E-5
60-61	0.08803222977673955	5.947495730720291E-5	9.292962079250456E-6	2.0444516574351E-5	7.434369663400365E-5
62-63	0.10100427554316527	6.226284593097804E-5	9.292962079250456E-6	2.3232405198126134E-5	7.620228904985373E-5
64-65	0.11683669503758427	6.319214213890309E-5	9.292962079250456E-6	2.416170140605118E-5	8.084877008947896E-5
66-67	0.13617720771692032	6.505073455475319E-5	1.1151554495100545E-5	2.416170140605118E-5	8.456595492117915E-5
68-69	0.159278582149729	6.969721559437842E-5	1.3010146910950636E-5	2.6949590029826317E-5	9.107102837665446E-5
70-71	0.1867718104611915	7.062651180230346E-5	1.486873932680073E-5	2.7878886237751364E-5	9.20003245845795E-5
72-73	0.21861600361815903	7.062651180230346E-5	1.672733174265082E-5	2.7878886237751364E-5	9.850539804005482E-5
74-75	0.2558045792669035	7.062651180230346E-5	1.7656627950575864E-5	2.7878886237751364E-5	9.943469424797988E-5
76-77	0.2988746706156056	7.34144004260786E-5	1.858592415850091E-5	2.9737478653601455E-5	1.0036399045590492E-4
78-79	0.35010398266988957	7.806088146570382E-5	1.858592415850091E-5	3.2525367277376593E-5	1.0036399045590492E-4
80-81	0.4081273793003135	8.363665871325409E-5	2.1373812782276047E-5	3.345466348530164E-5	1.0222258287175501E-4
82-83	0.4748935946548964	8.642454733702923E-5	2.230310899020109E-5	3.345466348530164E-5	1.0222258287175501E-4
84-85	0.5511107524440769	9.014173216872942E-5	2.6020293821901273E-5	3.6242552109076776E-5	1.0593976770345519E-4
86-87	0.6389561936830235	9.664680562420473E-5	2.9737478653601455E-5	4.0889033148702E-5	1.0686906391138023E-4
88	0.7109989529062047	9.850539804005482E-5	2.9737478653601455E-5	4.460621798040218E-5	1.0779836011930528E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	30635	0.0	43.95614	1
TCTCGAG	36695	0.0	36.37756	2
GATCCGG	49220	0.0	28.868565	1
CTCGAGG	46660	0.0	28.548101	3
TCGAGGG	54905	0.0	24.166842	4
ATCCGGC	60230	0.0	23.606667	2
TCCGGCG	75890	0.0	18.896431	3
TATCTCG	20515	0.0	18.489456	2
GCGCCGT	78625	0.0	18.051996	9
CGGCGCG	83140	0.0	17.050716	5
GGATCCG	32735	0.0	16.05283	2
CGAGGGC	84135	0.0	16.033432	5
AGGGCGC	87380	0.0	15.156659	7
CGTCGAC	75275	0.0	15.099864	12-13
CCGGCGC	95175	0.0	15.097097	4
TCGACTT	79040	0.0	14.446016	14-15
ATGGATC	34385	0.0	14.417424	1
GCCGTCG	80310	0.0	14.3141165	10-11
ATATCTC	28890	0.0	13.626803	1
GGGCGCG	97900	0.0	13.556754	8
>>END_MODULE