FastQCFastQC Report
Wed 25 May 2016
SRR491016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28250519
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA568160.20111488925212312No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT382940.13555149199205863No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377130.13349489260710573No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT369520.13080113678619495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG188200.041.8814542
ATATCTC198750.040.9876331
ATCTCGA222550.034.88933
TCTCGAG257800.029.8816364
ATGGATC255250.028.2468133
GATCCGG387200.027.598471
TATGGAT270700.026.7908172
GGATCCG260200.026.1197685
ATATGGA299200.024.8532031
TGGATCC289000.024.7528444
CTCGAGG317300.024.2486155
ATCCGGC477650.022.4255372
TCGAGGG380200.020.2226096
TCCGGCG590600.018.2163853
GCGCCGT621850.017.0425829
CGGCGCG676500.015.6045145
CGTCGAC597700.015.47031112-13
TCGACTT617200.015.04245414-15
CCGGCGC711150.015.0358684
GCCGTCG625350.014.95162410-11
Produced by FastQC (version 0.11.5)