##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491016_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28250519
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.173918680927596	31.0	31.0	34.0	28.0	34.0
2	31.46707276422072	34.0	31.0	34.0	28.0	34.0
3	31.529973590927657	34.0	31.0	34.0	28.0	34.0
4	34.946901010915944	37.0	35.0	37.0	32.0	37.0
5	34.660821381724	37.0	35.0	37.0	32.0	37.0
6	34.601203078782376	37.0	35.0	37.0	31.0	37.0
7	34.51994460703536	37.0	35.0	37.0	31.0	37.0
8	34.407923585403864	37.0	35.0	37.0	31.0	37.0
9	35.87068471202246	39.0	37.0	39.0	30.0	39.0
10-11	35.71269364998214	39.0	36.0	39.0	30.0	39.0
12-13	35.48686641827713	39.0	35.0	39.0	30.0	39.0
14-15	36.72886949439761	40.0	37.0	41.0	30.0	41.0
16-17	36.64771266680091	40.0	37.0	41.0	30.0	41.0
18-19	36.52775711837365	40.0	37.0	41.0	29.5	41.0
20-21	36.37932303119812	40.0	37.0	41.0	29.0	41.0
22-23	36.19461828294199	40.0	36.5	41.0	27.5	41.0
24-25	35.96174174711622	40.0	36.0	41.0	27.0	41.0
26-27	35.58968560896173	39.0	35.5	41.0	26.5	41.0
28-29	35.24888811777228	39.0	35.0	41.0	25.0	41.0
30-31	34.956669026151346	39.0	35.0	40.5	24.5	41.0
32-33	34.66983852579841	39.0	35.0	40.0	23.0	41.0
34-35	34.371435901761664	38.5	34.0	40.0	20.0	41.0
36-37	34.059480535561136	38.0	34.0	40.0	17.0	41.0
38-39	33.65215653206229	38.0	33.0	40.0	13.0	41.0
40-41	33.17677772574727	38.0	33.0	40.0	8.5	41.0
42-43	32.674655870924	38.0	32.5	40.0	2.0	41.0
44-45	32.17158812197397	38.0	31.5	40.0	2.0	41.0
46-47	31.63475676322973	37.5	31.0	40.0	2.0	41.0
48-49	31.181803137846778	37.0	30.0	40.0	2.0	41.0
50-51	30.657056034970545	37.0	29.5	40.0	2.0	41.0
52-53	30.123204709973646	36.0	28.0	39.0	2.0	40.0
54-55	29.5985160838992	36.0	27.0	39.0	2.0	40.0
56-57	28.98411862805069	35.0	26.0	39.0	2.0	40.0
58-59	28.374065287083752	34.5	24.5	38.0	2.0	40.0
60-61	27.780511943869065	34.0	22.5	38.0	2.0	40.0
62-63	27.16102613902421	34.0	21.0	38.0	2.0	40.0
64-65	26.502232348368537	33.0	18.0	37.0	2.0	39.5
66-67	25.83747703891741	32.5	11.5	36.5	2.0	39.0
68-69	25.182232138814868	32.0	7.5	36.0	2.0	39.0
70-71	24.39999895223164	31.0	2.0	35.0	2.0	38.0
72-73	23.675315575618274	31.0	2.0	35.0	2.0	37.5
74-75	22.963092005495547	30.0	2.0	34.5	2.0	37.0
76-77	21.985462815745084	29.0	2.0	34.0	2.0	36.0
78-79	22.012581131695313	29.0	2.0	34.0	2.0	36.0
80-81	21.42821406219121	29.0	2.0	34.0	2.0	35.0
82-83	20.546100020321752	28.0	2.0	34.0	2.0	35.0
84-85	19.704648470351998	26.0	2.0	33.0	2.0	35.0
86-87	19.085860688081517	25.5	2.0	33.0	2.0	35.0
88-89	18.413168745678618	25.0	2.0	32.0	2.0	34.0
90-91	17.604073326936046	22.5	2.0	32.0	2.0	34.0
92-93	16.87501165907784	19.0	2.0	32.0	2.0	34.0
94-95	16.00746327881622	10.0	2.0	31.0	2.0	34.0
96-97	14.932349508340007	2.0	2.0	31.0	2.0	34.0
98-99	13.907723624475715	2.0	2.0	30.0	2.0	34.0
100	13.170311986126697	2.0	2.0	29.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.31667264817091834
1101	2	0.3214425628373796
1101	3	0.2995647473506722
1101	4	0.31543295853422393
1101	5	0.4020464100288379
1101	6	0.3290839596022579
1101	7	0.34926306353027314
1101	8	0.35599468950437796
1101	9	0.4308850676634606
1101	10-11	0.39984801714053475
1101	12-13	0.41683552622612297
1101	14-15	0.4537660176083449
1101	16-17	0.46916194537488565
1101	18-19	0.4273398543647673
1101	20-21	0.44252092794873477
1101	22-23	0.4179900263413501
1101	24-25	0.43088395790228873
1101	26-27	0.4214191635178963
1101	28-29	0.4347677889259174
1101	30-31	0.45585052083191613
1101	32-33	0.35326514170679246
1101	34-35	0.3239267355739841
1101	36-37	0.3566941577604865
1101	38-39	0.31111593049362796
1101	40-41	0.20338348305089227
1101	42-43	0.21792156843752508
1101	44-45	0.21823999114209158
1101	46-47	0.16771642945548848
1101	48-49	0.1148575817564641
1101	50-51	0.05384364624892157
1101	52-53	0.008489775201908856
1101	54-55	0.03452784166621825
1101	56-57	0.06296348191332157
1101	58-59	-0.018982453786417608
1101	60-61	-0.054522849844417465
1101	62-63	-0.03671287228772968
1101	64-65	-0.13028145997803264
1101	66-67	-0.2712814334792668
1101	68-69	-0.30188943486730935
1101	70-71	-0.2652217677995381
1101	72-73	-0.2385683922713966
1101	74-75	-0.33062878899844605
1101	76-77	-0.21848136301247578
1101	78-79	-0.14912260801147426
1101	80-81	-0.2575446311458194
1101	82-83	-0.10122372610862129
1101	84-85	0.08885000093530238
1101	86-87	-0.3197730470398419
1101	88-89	-0.5668212716028052
1101	90-91	-0.6993080031844592
1101	92-93	-0.7435249657568868
1101	94-95	-0.5066560843203334
1101	96-97	-0.2030039309621543
1101	98-99	-0.07421367517340194
1101	100	0.07047100742351198
1102	1	0.19748462459961402
1102	2	0.19464054445921875
1102	3	0.2361322957942562
1102	4	0.23124130418957378
1102	5	0.24661619121517475
1102	6	0.25607254990204353
1102	7	0.24638528882830713
1102	8	0.2141269227311966
1102	9	0.2718936076502274
1102	10-11	0.25555183762008227
1102	12-13	0.26345203701564657
1102	14-15	0.31415334403227746
1102	16-17	0.32604240597359535
1102	18-19	0.2679421240395712
1102	20-21	0.2807671650025938
1102	22-23	0.28899844142159736
1102	24-25	0.26051972302480664
1102	26-27	0.26524947550032607
1102	28-29	0.2727745605658001
1102	30-31	0.24263266121987925
1102	32-33	0.25921427713081613
1102	34-35	0.1943679157437046
1102	36-37	0.22138799823958522
1102	38-39	0.17281443585783052
1102	40-41	0.17006990694542878
1102	42-43	0.21713023958747613
1102	44-45	0.10488150759579185
1102	46-47	0.06222775620253529
1102	48-49	0.03543718512983318
1102	50-51	0.005542045355447556
1102	52-53	-0.011384382372106927
1102	54-55	-0.01918553227157105
1102	56-57	-0.03439453254759428
1102	58-59	-0.14674101640384762
1102	60-61	-0.0801872386068112
1102	62-63	-0.25072372787568753
1102	64-65	-0.23701095856054621
1102	66-67	-0.4175084631797432
1102	68-69	-0.37769916512911905
1102	70-71	-0.2420254321792683
1102	72-73	-0.2434007681999013
1102	74-75	-0.35622875053714154
1102	76-77	-0.22431020379193
1102	78-79	-0.10332263839479339
1102	80-81	-0.223341126170709
1102	82-83	-0.057361597571734535
1102	84-85	0.15896196222544745
1102	86-87	-0.21781101884014475
1102	88-89	-0.3369285161575384
1102	90-91	-0.5165506384222027
1102	92-93	-0.6102089135442519
1102	94-95	-0.38684713657186265
1102	96-97	-0.056999419521785555
1102	98-99	0.032394568352058783
1102	100	0.06796696050830242
1103	1	0.04722285079895272
1103	2	0.04701833520224241
1103	3	-0.0029549269982354076
1103	4	0.050228570579974985
1103	5	0.1328713248314557
1103	6	0.02324632973537888
1103	7	0.07331702429755182
1103	8	0.06692251974932617
1103	9	0.056914001820601356
1103	10-11	0.06505416730687585
1103	12-13	0.04950222433059537
1103	14-15	-0.04762401758323165
1103	16-17	0.06250440380036082
1103	18-19	0.07618802501834665
1103	20-21	0.0937695679074011
1103	22-23	0.09556547115423086
1103	24-25	0.07399844723251903
1103	26-27	0.0806128667570789
1103	28-29	0.1387722680391903
1103	30-31	0.033772460586448005
1103	32-33	-0.10594601297562178
1103	34-35	0.0788357046581396
1103	36-37	-0.03762719787693669
1103	38-39	-0.004564446497781205
1103	40-41	0.00802263493540778
1103	42-43	0.032272805855242837
1103	44-45	0.004122336906917212
1103	46-47	-5.789722201612335E-4
1103	48-49	-0.02668820407345507
1103	50-51	-0.02745536191110176
1103	52-53	-0.01201041939617653
1103	54-55	-0.22767839908693333
1103	56-57	-0.11871685033112556
1103	58-59	-0.3297071684585404
1103	60-61	-0.33994535945991444
1103	62-63	-0.26801497494502513
1103	64-65	-0.22813902497913574
1103	66-67	-0.3418191437107403
1103	68-69	-0.24164275896403709
1103	70-71	-0.10617414627031252
1103	72-73	-0.1282494355548387
1103	74-75	-0.22786723809685583
1103	76-77	-0.1277239744366767
1103	78-79	-0.07792266347135524
1103	80-81	-0.15342412906797875
1103	82-83	0.0781487145284494
1103	84-85	0.3776703231025742
1103	86-87	0.053130317043766695
1103	88-89	-0.12221232318670161
1103	90-91	-0.5119795191917262
1103	92-93	-0.7739311142060252
1103	94-95	-0.5109415074040982
1103	96-97	-0.2834662571809492
1103	98-99	-0.4721531050292942
1103	100	-0.44612386690969785
1104	1	0.03718968491437735
1104	2	0.06341985074729095
1104	3	0.10461323416929957
1104	4	0.0726647826736766
1104	5	0.12643183920946655
1104	6	0.10576289621663193
1104	7	0.09855845845628153
1104	8	0.037900006494652416
1104	9	0.047300497795390584
1104	10-11	0.04902918756548047
1104	12-13	0.09247069369068583
1104	14-15	0.16012389272094651
1104	16-17	0.11946902460985598
1104	18-19	0.12311934343713204
1104	20-21	0.13540878912009902
1104	22-23	0.15659595600479292
1104	24-25	0.19578119251863058
1104	26-27	0.18632361736543857
1104	28-29	0.17567736365013076
1104	30-31	0.1902553677185992
1104	32-33	0.2389190164088788
1104	34-35	0.2508210616965627
1104	36-37	0.1893139361358962
1104	38-39	0.2319793060510733
1104	40-41	0.10613546089161474
1104	42-43	0.20512142912951958
1104	44-45	0.19878927488930742
1104	46-47	0.20999076736595157
1104	48-49	0.17043039087907275
1104	50-51	0.17983680197092866
1104	52-53	0.2262142373680014
1104	54-55	0.1172380210482089
1104	56-57	0.2992500413845356
1104	58-59	0.2492733645272125
1104	60-61	0.21568452454376086
1104	62-63	0.24391775718729036
1104	64-65	0.16920062755464826
1104	66-67	0.11425609557164407
1104	68-69	0.12163683843090567
1104	70-71	0.19998814460059577
1104	72-73	0.20872867477851287
1104	74-75	0.1992008567097514
1104	76-77	0.2477786569012821
1104	78-79	0.3234272137795706
1104	80-81	0.21540411265036497
1104	82-83	0.44864965176968497
1104	84-85	0.7200604550901701
1104	86-87	0.39426413757346523
1104	88-89	0.2538746347046619
1104	90-91	-7.210771263146398E-4
1104	92-93	-0.10691454134419232
1104	94-95	0.12205778121158062
1104	96-97	0.3104582976450594
1104	98-99	0.3274482997345398
1104	100	0.2612219807377869
1105	1	-0.04701750289985185
1105	2	-0.02447421575268649
1105	3	-0.014142937822239077
1105	4	-0.05817098390496511
1105	5	0.001695902190618881
1105	6	-0.020177549972778763
1105	7	-0.012626711332558216
1105	8	-0.005968072287977577
1105	9	-0.012251247646517527
1105	10-11	-0.006361322124526225
1105	12-13	-0.008319321226295529
1105	14-15	0.0064378431882659015
1105	16-17	0.0066974325453372785
1105	18-19	0.011496212891394464
1105	20-21	-0.0351161757952525
1105	22-23	-0.03030261167538839
1105	24-25	-0.014217621154692495
1105	26-27	0.0025051615627234014
1105	28-29	0.03923189999780874
1105	30-31	0.05929871387024832
1105	32-33	0.07538220665153261
1105	34-35	-0.0019984640192873826
1105	36-37	0.052433837773961045
1105	38-39	0.04806108052949298
1105	40-41	0.046461506894239335
1105	42-43	0.045773736103171814
1105	44-45	0.017455165058102295
1105	46-47	0.07405624088401552
1105	48-49	0.03958309390255721
1105	50-51	-0.008325868227082367
1105	52-53	0.089800377391974
1105	54-55	-0.013524392469371094
1105	56-57	0.14432440463858143
1105	58-59	0.1827544641059866
1105	60-61	-0.005566007791749428
1105	62-63	0.03934481694028591
1105	64-65	0.19863857643846927
1105	66-67	0.196779438456808
1105	68-69	0.11235983362731972
1105	70-71	0.2485437369579131
1105	72-73	0.3185823059031634
1105	74-75	0.32888575130054676
1105	76-77	0.3913886792505217
1105	78-79	0.48950671327636996
1105	80-81	0.34757444050895714
1105	82-83	0.6723755192171978
1105	84-85	1.0344403198966319
1105	86-87	0.6787201969790786
1105	88-89	0.5830948235406659
1105	90-91	0.4137471160681123
1105	92-93	0.23761821966294505
1105	94-95	0.09268640724186739
1105	96-97	0.3929107919114294
1105	98-99	0.4880710591956099
1105	100	0.37046266471259415
1106	1	-0.3071616550204581
1106	2	-0.3622952041054148
1106	3	-0.309439153065977
1106	4	-0.27364775753483883
1106	5	-0.28060355316036123
1106	6	-0.40383248642886116
1106	7	-0.3621983344365347
1106	8	-0.39919716903617086
1106	9	-0.41985309591235875
1106	10-11	-0.4091469902799858
1106	12-13	-0.4645813424469125
1106	14-15	-0.48893636128865126
1106	16-17	-0.459568002827794
1106	18-19	-0.43487598035138575
1106	20-21	-0.48663864428328907
1106	22-23	-0.5494948328055074
1106	24-25	-0.5545433310693184
1106	26-27	-0.5161986227781767
1106	28-29	-0.5085730002626505
1106	30-31	-0.5514297520068325
1106	32-33	-0.4941696680011418
1106	34-35	-0.6654257484131207
1106	36-37	-0.6262939247856139
1106	38-39	-0.5896034266132233
1106	40-41	-0.6258346791350107
1106	42-43	-0.5810568770412772
1106	44-45	-0.6017238884926428
1106	46-47	-0.6743910282307901
1106	48-49	-0.6476554842851918
1106	50-51	-0.6401468189584207
1106	52-53	-0.5420942225042609
1106	54-55	-0.6990192600182787
1106	56-57	-0.5706623611208599
1106	58-59	-0.5892761885934625
1106	60-61	-0.6762374849176567
1106	62-63	-0.5964161869218501
1106	64-65	-0.526521279721365
1106	66-67	-0.5672662743176815
1106	68-69	-0.5720464189634882
1106	70-71	-0.37368566164950323
1106	72-73	-0.4791740878928472
1106	74-75	-0.5798697954085696
1106	76-77	-0.4110629682123097
1106	78-79	-0.3637877660919848
1106	80-81	-0.40118814289704474
1106	82-83	-0.08895938747951249
1106	84-85	0.4253576877858265
1106	86-87	0.13199190555449647
1106	88-89	0.16042898316143095
1106	90-91	0.03745230979755121
1106	92-93	-0.12934993368952874
1106	94-95	-0.17386860604241505
1106	96-97	0.11138646686444176
1106	98-99	0.14230918397229608
1106	100	0.06215004478873709
1107	1	-0.3260174696793072
1107	2	-0.3192100481799436
1107	3	-0.31472006809479325
1107	4	-0.2923184413245252
1107	5	-0.3681514253703284
1107	6	-0.36186287499952385
1107	7	-0.4491381842248714
1107	8	-0.4161167493496194
1107	9	-0.42937894252180797
1107	10-11	-0.3623461597110307
1107	12-13	-0.4045478235907396
1107	14-15	-0.45541444511187024
1107	16-17	-0.5317770594927183
1107	18-19	-0.5105148378115985
1107	20-21	-0.4696200673744073
1107	22-23	-0.47218159722669384
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2207	94-95	0.6731999320402373
2207	96-97	0.5803904641227593
2207	98-99	0.5175584404200873
2207	100	0.37229856752093937
2208	1	-0.5708760011650611
2208	2	-0.5252047608751411
2208	3	-0.9361461735440955
2208	4	-0.6736149528050532
2208	5	-1.2622258365216013
2208	6	-0.8178139682266092
2208	7	-0.7097975244115347
2208	8	-0.7194333218781495
2208	9	-0.6652382577942504
2208	10-11	-0.6250738994414959
2208	12-13	-0.5779201595019003
2208	14-15	-0.7381458425031724
2208	16-17	-0.6736449300466774
2208	18-19	-0.7138570537796411
2208	20-21	-0.8116649251114083
2208	22-23	-0.7834972693171807
2208	24-25	-0.7736524001427298
2208	26-27	-0.7944713240827781
2208	28-29	-0.8262471977442161
2208	30-31	-0.8308091283145131
2208	32-33	-0.8509887304519381
2208	34-35	-0.891816201164211
2208	36-37	-0.7806833497352414
2208	38-39	-0.7859117786160255
2208	40-41	-0.8322021423273256
2208	42-43	-0.8734075633758245
2208	44-45	-0.8599262001103831
2208	46-47	-0.6644670237083083
2208	48-49	-0.7026978728090505
2208	50-51	-0.7115302755517305
2208	52-53	-0.5533612801555066
2208	54-55	-0.511961282203174
2208	56-57	-0.7069370065019314
2208	58-59	-0.6401611056466905
2208	60-61	-0.5028502849297922
2208	62-63	-0.5426449557889939
2208	64-65	-0.7104334835323662
2208	66-67	-0.25083331973034717
2208	68-69	-0.3191446768518382
2208	70-71	-0.1960887031211911
2208	72-73	-0.17363000262000838
2208	74-75	-0.24070643340269982
2208	76-77	-0.32899153625373856
2208	78-79	-0.44877554185918456
2208	80-81	-0.44616345022773984
2208	82-83	-0.38233332938660425
2208	84-85	-0.2536412504034615
2208	86-87	-0.5311456807394208
2208	88-89	-1.1763448841139201
2208	90-91	-2.377589947240587
2208	92-93	-2.4669060421652915
2208	94-95	-2.581496544733117
2208	96-97	-3.3889524075186372
2208	98-99	-3.332081666695201
2208	100	-3.2007818984653387
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	508103.0
3	213200.0
4	191204.0
5	150386.0
6	142297.0
7	150895.0
8	165121.0
9	184932.0
10	206123.0
11	229755.0
12	256564.0
13	303908.0
14	381252.0
15	467442.0
16	517044.0
17	502167.0
18	477273.0
19	457198.0
20	463582.0
21	481730.0
22	507313.0
23	537678.0
24	570822.0
25	612577.0
26	663829.0
27	729376.0
28	811762.0
29	911764.0
30	1033007.0
31	1177367.0
32	1365097.0
33	1640800.0
34	2055621.0
35	2681494.0
36	3215569.0
37	2553641.0
38	710000.0
39	22615.0
40	11.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.251381732145028	28.106137829464085	27.612524417473406	22.02995602091748
2	23.68612143678215	27.692904604439466	27.68397379474599	20.93700016403239
3	23.45576397532633	26.697047337613444	27.179585531415196	22.66760315564503
4	24.395884301519132	26.11638689429052	26.815433770189845	22.6722950340005
5	24.903486432803827	26.189851358256032	26.17318090542472	22.73348130351542
6	24.81309514496573	25.993896738709125	25.920099736393766	23.27290837993138
7	24.590198926160994	26.40937343131814	25.835586972722453	23.164840669798416
8	24.179179140379876	26.093646998172666	26.290274239923395	23.436899621524063
9	24.229269937219623	26.052416284923435	26.201944281653233	23.516369496203712
10-11	24.17702584634984	26.108069032034205	26.29049407342505	23.424411048190905
12-13	23.81129571521422	26.16024899138352	26.451876147625303	23.57657914577696
14-15	23.324970149332188	26.353783514955314	27.20502688024694	23.116219455465554
16-17	23.125128240036002	26.330760559231248	27.430271358172796	23.11383984255995
18-19	22.68306124530216	26.26047340722778	28.321088171360785	22.735377176109274
20-21	22.293652826484887	26.102908069295495	29.344408678700724	22.2590304255189
22-23	22.276222241109082	26.01706302347394	29.734535741174373	21.972178994242604
24-25	22.118424626130416	25.816152480055145	30.23015808622272	21.835264807591717
26-27	22.136596939960818	25.86218138633275	30.43927368043257	21.561947993273854
28-29	22.206164867782014	25.9403684923025	30.38289312587671	21.470573514038776
30-31	22.14283978539645	25.88797197530388	30.37368190660902	21.595506332690647
32-33	22.153619549431994	25.997918834694683	30.23882676279328	21.609634853080046
34-35	22.24574136082121	26.057813459329854	30.231974532145696	21.464470647703237
36-37	22.269237661573968	26.081406775918758	30.042780791253932	21.60657477125334
38-39	22.31740789510945	26.204770551921392	29.823094540254296	21.654727012714865
40-41	22.402487962790644	26.407822750261523	29.462497419286382	21.72719186766145
42-43	22.55290366749122	26.570946498737797	28.936923877376525	21.939225956394463
44-45	22.6112432251885	26.871606886369147	28.441930609378264	22.075219279064093
46-47	22.851312092157286	27.085479062728236	27.947981970873155	22.11522687424133
48-49	22.92966031712646	27.198103806810902	27.55564087952152	22.316594996541124
50-51	22.954821117947365	27.39778660827797	27.225444187258717	22.421948086515947
52-53	23.09953611075502	27.503330883750245	27.02253567477867	22.37459733071607
54-55	23.1374688360679	27.539711685210616	26.882328224197412	22.440491254524073
56-57	23.111442945169493	27.66930082762918	26.823016603097326	22.396239624104005
58-59	23.18080864402681	27.821324089535054	26.65915359379066	22.33871367264748
60-61	23.164169529806877	27.848015344529415	26.568952928580213	22.418862197083495
62-63	23.156768338187355	28.007315744625295	26.461365637523613	22.374550279663737
64-65	23.225819903852944	28.15835453049408	26.295785694629515	22.32003987102346
66-67	23.235463684417397	28.250075271706038	26.136720463052455	22.377740580824113
68-69	23.207534783512216	28.414630867620023	26.01374014463983	22.364094204227936
70-71	23.261321274389342	28.53461942213408	25.86783284147296	22.336226462003616
72-73	23.25449680347454	28.634820965882174	25.66305049931954	22.447631731323746
74-75	23.292393650261385	28.787656453317446	25.54141340748659	22.378536488934575
76-77	22.79894830155927	29.276292562798677	25.28335991514589	22.641399220496158
78-79	23.049529094483002	29.239777737870988	25.156424040569448	22.55426912707656
80-81	22.969996306386438	29.394736801579885	24.932521678849408	22.70274521318427
82-83	22.953119046366126	29.182494421820916	24.66243075697296	23.201955774840002
84-85	22.84888027580895	29.648592899883024	24.592545708091244	22.909981116216784
86-87	22.83640762041367	29.780069789439928	24.424137468448716	22.959385121697686
88-89	23.0868112216241	29.754103370045787	24.32603146215705	22.833053946173067
90-91	22.860264686751947	29.789693057171807	24.270396410667114	23.079645845409132
92-93	22.821095887840972	29.927965226496266	24.132967696386352	23.117971189276414
94-95	22.890800987827454	29.972150271998043	23.951681143163476	23.18536759701103
96-97	22.78725873933281	30.016078439563014	23.69546755797814	23.501195263126036
98-99	22.748947962116652	30.029673489664827	23.59799080038987	23.623387747828648
100	23.468005135543972	30.16178436117194	22.868778100825033	23.501432402459056
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5017.0
1	3711.0
2	2135.0
3	1785.5
4	1787.5
5	2054.0
6	2444.5
7	2909.0
8	3510.5
9	4288.5
10	5379.0
11	6988.0
12	9041.0
13	12648.0
14	20335.0
15	29294.5
16	47165.0
17	54521.0
18	47226.5
19	48512.0
20	59818.5
21	68339.0
22	73042.5
23	84824.5
24	98522.0
25	114281.0
26	133220.0
27	159056.5
28	189063.0
29	218124.5
30	247889.0
31	284043.5
32	329469.0
33	382164.5
34	443595.0
35	516235.0
36	596880.0
37	691815.0
38	786042.5
39	859604.0
40	922141.0
41	999594.5
42	1114013.0
43	1221032.5
44	1292374.5
45	1342017.0
46	1332384.0
47	1272622.0
48	1215783.5
49	1182312.0
50	1142659.5
51	1059417.5
52	944639.0
53	826790.5
54	728608.0
55	650250.5
56	573859.5
57	501348.5
58	448026.5
59	402437.0
60	354605.5
61	308214.0
62	268744.5
63	236158.5
64	206295.5
65	178350.0
66	153498.0
67	129997.5
68	107877.0
69	89743.0
70	74411.5
71	61658.5
72	51523.5
73	43067.5
74	35894.0
75	29176.5
76	23298.0
77	18697.0
78	15188.5
79	12230.5
80	9542.5
81	7282.0
82	5445.5
83	4008.5
84	2887.5
85	1955.0
86	1322.5
87	929.5
88	665.0
89	461.5
90	312.0
91	202.5
92	120.5
93	67.5
94	50.0
95	32.0
96	13.0
97	6.5
98	2.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07339688166436871
2	3.539757977543705E-6
3	0.0011327225528139854
4	1.8760717280981632E-4
5	0.010392729422068318
6	1.7344814089964152E-4
7	0.001019450297532587
8	3.893733775298075E-4
9	0.001030069571465218
10-11	4.07072167417526E-5
12-13	2.2477463157402522E-4
14-15	0.0011097141259599514
16-17	9.681238068582032E-4
18-19	1.486698350568356E-4
20-21	8.14144334835052E-5
22-23	0.0015079368984336182
24-25	0.005773345261373782
26-27	0.0026548184831577783
28-29	1.8052765685472894E-4
30-31	2.6548184831577785E-5
32-33	0.0
34-35	1.415903191017482E-5
36-37	3.7698422460840454E-4
38-39	4.212311993277009E-4
40-41	3.415866448329675E-4
42-43	6.566251048343572E-4
44-45	3.6105531370945787E-4
46-47	4.300805942715601E-4
48-49	7.787467550596149E-5
50-51	2.63711969327006E-4
52-53	4.725576900020845E-4
54-55	3.982227724736668E-4
56-57	0.002796408802259527
58-59	0.0038671855904664977
60-61	0.004364521586311388
62-63	0.0075396844921680915
64-65	0.008621080554307691
66-67	0.004221161388220868
68-69	0.003920281960129653
70-71	0.008833466032960315
72-73	0.008585682974532256
74-75	0.012159068652862625
76-77	0.012206855385559465
78-79	0.014659907663997253
80-81	0.011021036463082324
82-83	0.008061798793855787
84-85	0.0162474891169256
86-87	0.008008702424192632
88-89	0.001042458724386621
90-91	5.097251487662935E-4
92-93	9.69893685846975E-4
94-95	0.003720285634398434
96-97	0.005341494788113451
98-99	0.0037556832141738706
100	0.003985767482714211
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.8250519E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.58870704860759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.83662901145149	30.00955659659816
2	10.615071437870295	7.980136211562966
3	3.53988643159387	3.9917926218756867
4	1.6832838511787716	2.530898542464433
5	0.8748257379952532	1.6441784192042759
6	0.5532506430365702	1.2477585807333245
7	0.40014831516525134	1.0528740456318373
8	0.28503009390743794	0.8571130159939038
9	0.21008619409681495	0.7107181564375882
>10	1.593809215570809	12.035630540008157
>50	0.14899118908818157	3.8794281194474123
>100	0.20459669947776007	18.522759486023855
>500	0.05036788011908369	12.36706544099388
>1k	0.0038152572836972474	1.7870279950013213
>5k	7.56516962788397E-5	0.23477169852223131
>10k+	1.3239046848787474E-4	1.1482905295008663
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56816	0.20111488925212312	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	38294	0.13555149199205863	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37713	0.13349489260710573	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	36952	0.13080113678619495	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	3.5397579775437044E-6	0.0	0.0	0.0
2	0.0	3.5397579775437044E-6	0.0	0.0	0.0
3	3.5397579775437044E-6	7.079515955087409E-6	0.0	0.0	0.0
4	3.5397579775437044E-6	7.079515955087409E-6	0.0	0.0	0.0
5	3.5397579775437044E-6	7.079515955087409E-6	0.0	0.0	0.0
6	3.5397579775437044E-6	1.0619273932631113E-5	0.0	0.0	0.0
7	7.079515955087409E-6	1.0619273932631113E-5	0.0	0.0	3.5397579775437044E-6
8	7.079515955087409E-6	1.4159031910174818E-5	0.0	0.0	7.079515955087409E-6
9	1.0619273932631113E-5	1.4159031910174818E-5	0.0	0.0	7.079515955087409E-6
10-11	2.300842685403408E-5	1.592891089894667E-5	0.0	0.0	1.0619273932631113E-5
12-13	1.2389152921402966E-4	1.7698789887718522E-5	0.0	0.0	1.0619273932631113E-5
14-15	4.920263588785749E-4	1.9468668876490373E-5	0.0	0.0	1.592891089894667E-5
16-17	0.001173429769555738	2.1238547865262226E-5	0.0	0.0	1.7698789887718522E-5
18-19	0.002361018571021651	2.1238547865262226E-5	0.0	0.0	1.7698789887718522E-5
20-21	0.003991077119680527	2.1238547865262226E-5	0.0	0.0	2.477830584280593E-5
22-23	0.006160948759914818	2.300842685403408E-5	0.0	0.0	2.8318063820349635E-5
24-25	0.008828156395993998	2.477830584280593E-5	0.0	0.0	3.008794280912149E-5
26-27	0.011631644714208612	2.477830584280593E-5	3.5397579775437044E-6	0.0	3.36277007866652E-5
28-29	0.015166093054786	2.477830584280593E-5	3.5397579775437044E-6	0.0	3.5397579775437044E-5
30-31	0.018036836774573947	2.477830584280593E-5	3.5397579775437044E-6	0.0	3.893733775298075E-5
32-33	0.02004564942683	2.477830584280593E-5	3.5397579775437044E-6	0.0	3.893733775298075E-5
34-35	0.022093399416839035	2.477830584280593E-5	3.5397579775437044E-6	0.0	4.247709573052445E-5
36-37	0.024367693917410865	2.8318063820349635E-5	3.5397579775437044E-6	0.0	5.309636966315557E-5
38-39	0.02662959926506129	2.8318063820349635E-5	3.5397579775437044E-6	0.0	5.663612764069927E-5
40-41	0.028857876911925054	2.8318063820349635E-5	3.5397579775437044E-6	0.0	5.663612764069927E-5
42-43	0.03129677015845266	2.8318063820349635E-5	3.5397579775437044E-6	0.0	6.017588561824298E-5
44-45	0.03427901625453324	3.185782179789334E-5	3.5397579775437044E-6	3.5397579775437044E-6	6.017588561824298E-5
46-47	0.03786125132780747	3.185782179789334E-5	3.5397579775437044E-6	3.5397579775437044E-6	6.017588561824298E-5
48-49	0.04207356332108447	3.36277007866652E-5	3.5397579775437044E-6	7.079515955087409E-6	6.548552258455854E-5
50-51	0.046931881145263206	3.71674587642089E-5	3.5397579775437044E-6	8.849394943859261E-6	7.079515955087409E-5
52-53	0.053048582930458735	3.893733775298075E-5	3.5397579775437044E-6	1.0619273932631113E-5	7.43349175284178E-5
54-55	0.06028030847858051	3.893733775298075E-5	3.5397579775437044E-6	1.2389152921402965E-5	7.78746755059615E-5
56-57	0.06919872870300188	3.893733775298075E-5	3.5397579775437044E-6	1.4159031910174818E-5	7.78746755059615E-5
58-59	0.08031002899451156	3.893733775298075E-5	3.5397579775437044E-6	1.7698789887718522E-5	8.141443348350521E-5
60-61	0.09323014561254608	3.893733775298075E-5	3.5397579775437044E-6	1.7698789887718522E-5	9.203370741613632E-5
62-63	0.10865464099969278	4.07072167417526E-5	5.309636966315557E-6	1.7698789887718522E-5	9.203370741613632E-5
64-65	0.1274348269495509	4.247709573052445E-5	7.079515955087409E-6	1.7698789887718522E-5	9.203370741613632E-5
66-67	0.15044679356156254	4.601685370806816E-5	7.079515955087409E-6	1.7698789887718522E-5	9.557346539368003E-5
68-69	0.17862326706281043	4.955661168561186E-5	8.849394943859261E-6	2.1238547865262226E-5	9.734334438245187E-5
70-71	0.21195185830037316	4.955661168561186E-5	1.0619273932631113E-5	2.477830584280593E-5	9.911322337122372E-5
72-73	0.25136352362234476	5.1326490674383716E-5	1.0619273932631113E-5	2.477830584280593E-5	9.911322337122372E-5
74-75	0.29764231942075114	5.486624865192742E-5	1.0619273932631113E-5	2.477830584280593E-5	1.0088310235999558E-4
76-77	0.3511652299202007	6.371564359578669E-5	1.0619273932631113E-5	2.654818483157778E-5	1.0619273932631114E-4
78-79	0.41491273133778533	7.079515955087409E-5	1.0619273932631113E-5	3.36277007866652E-5	1.0619273932631114E-4
80-81	0.487976875752265	7.079515955087409E-5	1.0619273932631113E-5	4.07072167417526E-5	1.0796261831508299E-4
82-83	0.5703948306224038	7.079515955087409E-5	1.4159031910174818E-5	4.601685370806816E-5	1.0973249730385485E-4
84-85	0.665617859976307	7.079515955087409E-5	1.4159031910174818E-5	4.601685370806816E-5	1.115023762926267E-4
86-87	0.7754158428027464	7.43349175284178E-5	1.4159031910174818E-5	5.1326490674383716E-5	1.150421342701704E-4
88	0.8657894037274148	7.43349175284178E-5	1.4159031910174818E-5	5.309636966315557E-5	1.1681201325894225E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	18820	0.0	41.881454	2
ATATCTC	19875	0.0	40.987633	1
ATCTCGA	22255	0.0	34.8893	3
TCTCGAG	25780	0.0	29.881636	4
ATGGATC	25525	0.0	28.246813	3
GATCCGG	38720	0.0	27.59847	1
TATGGAT	27070	0.0	26.790817	2
GGATCCG	26020	0.0	26.119768	5
ATATGGA	29920	0.0	24.853203	1
TGGATCC	28900	0.0	24.752844	4
CTCGAGG	31730	0.0	24.248615	5
ATCCGGC	47765	0.0	22.425537	2
TCGAGGG	38020	0.0	20.222609	6
TCCGGCG	59060	0.0	18.216385	3
GCGCCGT	62185	0.0	17.042582	9
CGGCGCG	67650	0.0	15.604514	5
CGTCGAC	59770	0.0	15.470311	12-13
TCGACTT	61720	0.0	15.042454	14-15
CCGGCGC	71115	0.0	15.035868	4
GCCGTCG	62535	0.0	14.951624	10-11
>>END_MODULE