FastQCFastQC Report
Wed 25 May 2016
SRR491015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32500760
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA191600.044.2581021
TCTCGAG231900.036.711692
CTCGAGG297350.028.59813
GATCCGG322050.027.4845641
TCGAGGG359400.023.5953124
ATCCGGC403400.021.954912
TATCTCG132050.020.5767152
GCGCCGT506850.017.4319299
TCCGGCG511300.017.4036943
GGATCCG216550.017.1496622
ATATCTC166750.016.7255881
ATGGATC212350.016.23471
CGAGGGC528550.016.2131425
CGGCGCG554350.015.9758315
AGGGCGC550300.015.2873867
TGGATCC255750.015.2083611
CGTCGAC489300.014.71954212-13
CCGGCGC613150.014.5127974
TCGACTT510850.014.12164814-15
GCCGTCG516700.014.07080410-11
Produced by FastQC (version 0.11.5)