##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491015_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32500760
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.672680423473174	33.0	31.0	34.0	30.0	34.0
2	31.962618720300693	34.0	31.0	34.0	30.0	34.0
3	32.05139513660604	34.0	31.0	34.0	30.0	34.0
4	35.410037611428166	37.0	35.0	37.0	33.0	37.0
5	35.206671813212985	37.0	35.0	37.0	32.0	37.0
6	35.15431894515697	37.0	35.0	37.0	32.0	37.0
7	35.08134659004897	37.0	35.0	37.0	32.0	37.0
8	34.99572917679463	37.0	35.0	37.0	32.0	37.0
9	36.55959808324482	39.0	37.0	39.0	32.0	39.0
10-11	36.41470996370546	39.0	37.0	39.0	32.0	39.0
12-13	36.19933093872267	39.0	37.0	39.0	32.0	39.0
14-15	37.53404003475611	40.0	38.0	41.0	32.0	41.0
16-17	37.457006589999736	40.0	38.0	41.0	32.0	41.0
18-19	37.341744239211636	40.0	38.0	41.0	32.0	41.0
20-21	37.195381661844216	40.0	38.0	41.0	31.0	41.0
22-23	37.00120226419321	40.0	38.0	41.0	31.0	41.0
24-25	36.803971768660176	40.0	37.0	41.0	30.0	41.0
26-27	36.483862761978486	40.0	37.0	41.0	30.0	41.0
28-29	36.16734442517652	40.0	36.0	41.0	29.0	41.0
30-31	35.873274240356224	40.0	36.0	41.0	27.5	41.0
32-33	35.653728835879534	39.5	35.0	41.0	26.5	41.0
34-35	35.36615725293808	39.0	35.0	41.0	25.5	41.0
36-37	35.07420637240483	39.0	35.0	40.5	24.5	41.0
38-39	34.713287996957604	39.0	35.0	40.0	23.5	41.0
40-41	34.251980538301254	38.5	34.0	40.0	19.5	41.0
42-43	33.8040993195236	38.0	33.5	40.0	14.5	41.0
44-45	33.33163032187555	38.0	33.0	40.0	8.5	41.0
46-47	32.81728913416178	38.0	33.0	40.0	2.0	41.0
48-49	32.2824096728815	38.0	32.0	40.0	2.0	41.0
50-51	31.775275670476628	37.0	31.0	40.0	2.0	41.0
52-53	31.217914011856955	37.0	30.5	40.0	2.0	41.0
54-55	30.611862476446703	36.0	29.0	39.0	2.0	40.0
56-57	30.081600030276213	36.0	28.0	39.0	2.0	40.0
58-59	29.477238747647746	35.0	27.5	39.0	2.0	40.0
60-61	28.87579450449774	35.0	26.0	38.0	2.0	40.0
62-63	28.229922084898938	34.0	25.5	38.0	2.0	40.0
64-65	27.53159867646172	34.0	22.5	37.5	2.0	39.5
66-67	26.841793145760285	33.5	21.0	37.0	2.0	39.0
68-69	26.130240692833027	32.5	18.5	36.0	2.0	39.0
70-71	25.419631587076736	32.0	12.0	36.0	2.0	38.5
72-73	24.618214281758334	31.0	7.5	35.0	2.0	38.0
74-75	23.83690681387143	30.5	2.0	34.5	2.0	37.0
76-77	23.027198933194178	29.5	2.0	34.0	2.0	36.0
78-79	23.167982194877904	30.0	2.0	34.0	2.0	36.0
80-81	22.509481209054805	30.0	2.0	34.0	2.0	35.5
82-83	21.726401951831278	29.0	2.0	34.0	2.0	35.0
84-85	21.06788093570735	29.0	2.0	34.0	2.0	35.0
86-87	20.183014166437953	27.5	2.0	33.0	2.0	35.0
88-89	19.23422153820403	26.0	2.0	32.5	2.0	34.0
90-91	18.483276114158564	25.0	2.0	32.0	2.0	34.0
92-93	17.614091362786596	22.5	2.0	32.0	2.0	34.0
94-95	16.61894991070978	18.5	2.0	31.0	2.0	34.0
96-97	15.736425148211918	5.0	2.0	31.0	2.0	34.0
98-99	14.903170679701029	2.0	2.0	31.0	2.0	34.0
100	14.152025398790675	2.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09268337298998475
1101	2	0.0697377364462568
1101	3	0.08298805613458171
1101	4	0.12256533373008693
1101	5	0.12625701698116387
1101	6	0.09641668933266345
1101	7	0.10449863657833447
1101	8	0.13438744923549706
1101	9	0.09866245556089126
1101	10-11	0.12051050312015832
1101	12-13	0.07221658367937067
1101	14-15	0.09820500129998067
1101	16-17	0.11578122274662661
1101	18-19	0.11072188769286839
1101	20-21	0.09157512823541936
1101	22-23	0.09630793605558807
1101	24-25	0.05384480921460977
1101	26-27	0.06231292341290384
1101	28-29	0.08630140854427992
1101	30-31	0.035835609224086795
1101	32-33	0.020480361935696578
1101	34-35	-8.432692875928183E-4
1101	36-37	-0.02861744613441175
1101	38-39	-0.043590930286242724
1101	40-41	-0.034786540396609666
1101	42-43	-0.16385249255406364
1101	44-45	-0.2161951442819543
1101	46-47	-0.10177002752633513
1101	48-49	-0.22446164088476905
1101	50-51	-0.28939023096178573
1101	52-53	-0.3249417838558628
1101	54-55	-0.34537555180677515
1101	56-57	-0.35844182855900186
1101	58-59	-0.3545395426834581
1101	60-61	-0.43250557554321745
1101	62-63	-0.3459765882118333
1101	64-65	-0.34186813563454166
1101	66-67	-0.4559990778027725
1101	68-69	-0.5979110602522582
1101	70-71	-0.7232170144472647
1101	72-73	-0.6220197603192332
1101	74-75	-0.5685256919939796
1101	76-77	-0.6975953495017286
1101	78-79	-0.6619708682553131
1101	80-81	-0.7678180752985533
1101	82-83	-0.7542103434837841
1101	84-85	-0.7324703926542497
1101	86-87	-0.4207759688530963
1101	88-89	-0.5644359466535178
1101	90-91	-0.5366393237528477
1101	92-93	-0.4518258564933362
1101	94-95	-0.34415152985938846
1101	96-97	-0.4771717536206328
1101	98-99	-0.2862569083503246
1101	100	-0.28983816714736044
1102	1	0.09390136955816075
1102	2	0.11320441899094646
1102	3	0.07217443058097928
1102	4	0.08037514726972006
1102	5	0.0859349198405468
1102	6	0.09147343420459464
1102	7	0.10219441020496589
1102	8	0.13593884681113622
1102	9	0.11865263828997286
1102	10-11	0.13300125899257864
1102	12-13	0.09592859671231935
1102	14-15	0.09756543086135139
1102	16-17	0.1079591702417062
1102	18-19	0.08049709728810939
1102	20-21	0.03588411272816927
1102	22-23	0.060508877091542956
1102	24-25	-0.023965618650265696
1102	26-27	0.007430109168211629
1102	28-29	0.048446811127604406
1102	30-31	-0.021072614792984723
1102	32-33	-0.026525289514793826
1102	34-35	0.01945547400663372
1102	36-37	0.013331594409159209
1102	38-39	-0.021089689749018703
1102	40-41	-0.025130754901567798
1102	42-43	-0.08867506965623306
1102	44-45	-0.07421885804541262
1102	46-47	0.011487879525354572
1102	48-49	-0.10482016610907863
1102	50-51	-0.17837656545317415
1102	52-53	-0.2598409656914171
1102	54-55	-0.22218495043178166
1102	56-57	-0.2599170054519462
1102	58-59	-0.41291441871620194
1102	60-61	-0.4792077718359735
1102	62-63	-0.30896788712747636
1102	64-65	-0.2771444712787101
1102	66-67	-0.3766811255595144
1102	68-69	-0.4565495719607746
1102	70-71	-0.5010946516315222
1102	72-73	-0.48982989320814596
1102	74-75	-0.5542975225248945
1102	76-77	-0.6548262862076264
1102	78-79	-0.6872086805082169
1102	80-81	-0.6667469602344163
1102	82-83	-0.6083198888097101
1102	84-85	-0.5760094872474539
1102	86-87	-0.3130212862447088
1102	88-89	-0.4301070723412117
1102	90-91	-0.385246123095083
1102	92-93	-0.37987973764638383
1102	94-95	-0.23132840584938563
1102	96-97	-0.4018493827004992
1102	98-99	-0.3210724592180938
1102	100	-0.2922411630514592
1103	1	0.009828950570643258
1103	2	0.006851239793331132
1103	3	0.0026330134482108747
1103	4	-0.09139710950388746
1103	5	-0.013194321187228297
1103	6	0.010167264891350669
1103	7	0.01572646380274989
1103	8	0.004653669147252515
1103	9	0.03933127121210589
1103	10-11	0.009247922810814657
1103	12-13	-0.08123634896168852
1103	14-15	-0.06718151547167395
1103	16-17	-0.008792187195119539
1103	18-19	-0.0013372201843537823
1103	20-21	-0.0800886825920486
1103	22-23	-0.09303295646481757
1103	24-25	-0.08133539563893066
1103	26-27	-0.06662710199322674
1103	28-29	-0.014448972254946568
1103	30-31	-0.07028734084812527
1103	32-33	-0.057317429810026965
1103	34-35	-0.00813918356305976
1103	36-37	-0.012605905847372867
1103	38-39	-0.12709482750861412
1103	40-41	-0.07120414398700348
1103	42-43	-0.12177133151962494
1103	44-45	-0.12233574319592577
1103	46-47	-0.16511602363471667
1103	48-49	-0.22942088994258825
1103	50-51	-0.3260977553350166
1103	52-53	-0.5016729473038204
1103	54-55	-0.365051094997618
1103	56-57	-0.32433665948865453
1103	58-59	-0.40471458773549784
1103	60-61	-0.3740112160146687
1103	62-63	-0.2093359641988144
1103	64-65	-0.18569595302201947
1103	66-67	-0.26196534126349746
1103	68-69	-0.2974105432947738
1103	70-71	-0.3109970721300144
1103	72-73	-0.3114298585083688
1103	74-75	-0.31894906408225765
1103	76-77	-0.45823436881629576
1103	78-79	-0.3658171885579087
1103	80-81	-0.40353057585020125
1103	82-83	-0.35811023426697375
1103	84-85	-0.2407414238399923
1103	86-87	0.03439369647028556
1103	88-89	-0.09524787603844231
1103	90-91	-0.14356334417694327
1103	92-93	-0.15123275394262947
1103	94-95	-0.32379782950026126
1103	96-97	-0.5330289731625744
1103	98-99	-0.5143690041683584
1103	100	-0.4776430944980774
1104	1	-0.0025106212863796884
1104	2	-0.0056578090975385464
1104	3	-0.005044785715000444
1104	4	0.028948105837201865
1104	5	-0.025216777732786966
1104	6	0.008585362929579787
1104	7	-0.016799293892447054
1104	8	-0.02627491052172104
1104	9	-0.046793027198198445
1104	10-11	-0.046185794900338806
1104	12-13	-0.05721667134130115
1104	14-15	-0.003918500662351221
1104	16-17	0.012275752545043872
1104	18-19	0.018089911528043956
1104	20-21	0.006125990512003909
1104	22-23	0.011207912460641012
1104	24-25	-0.0934525273486031
1104	26-27	-0.018882497960937883
1104	28-29	0.02241711429668669
1104	30-31	-0.021869128731296428
1104	32-33	0.05335033220440266
1104	34-35	0.07628901970990398
1104	36-37	0.07206177767611166
1104	38-39	0.11417956962709752
1104	40-41	0.1705131245085738
1104	42-43	0.16506336679520928
1104	44-45	0.11999806078783593
1104	46-47	0.14987871843602107
1104	48-49	0.11340192738658317
1104	50-51	0.12744720133166965
1104	52-53	0.04157259302057881
1104	54-55	0.14268533329445887
1104	56-57	0.09618650867302492
1104	58-59	0.028094497272590502
1104	60-61	0.10165287325637351
1104	62-63	0.14914649503111832
1104	64-65	0.23208293196639218
1104	66-67	0.17336912669941995
1104	68-69	0.04690868018910166
1104	70-71	0.011275485400950913
1104	72-73	0.16739114539635125
1104	74-75	0.19464738464728626
1104	76-77	0.006000090305988692
1104	78-79	0.07494101309441348
1104	80-81	-0.03970551554898094
1104	82-83	0.04123002491520111
1104	84-85	0.15697969154937397
1104	86-87	0.42882391219578153
1104	88-89	0.292059163911631
1104	90-91	0.24157814068854933
1104	92-93	0.238769889179828
1104	94-95	0.40934164625545577
1104	96-97	-0.050361637734336995
1104	98-99	0.062185381411554275
1104	100	0.09971411139657249
1105	1	0.02409938251529198
1105	2	0.034093456403290645
1105	3	0.02343367488096959
1105	4	0.04289960985055785
1105	5	0.029323021393707904
1105	6	0.025271633411250605
1105	7	-0.02941474045899639
1105	8	0.01521085099257391
1105	9	-0.02927587506604823
1105	10-11	0.030732941902712696
1105	12-13	0.032740221713673634
1105	14-15	0.03232995226879609
1105	16-17	0.024081022089610826
1105	18-19	0.0192080838902271
1105	20-21	0.04926957977086488
1105	22-23	0.05115794136520435
1105	24-25	0.04007173429647537
1105	26-27	0.0500955581736946
1105	28-29	0.07555879514078612
1105	30-31	0.07077440775584876
1105	32-33	0.026195646442644716
1105	34-35	0.051572504640759576
1105	36-37	0.09387137105495214
1105	38-39	0.09186902579115497
1105	40-41	0.2043182256833589
1105	42-43	0.19838260885676107
1105	44-45	0.14536062821105844
1105	46-47	0.2000699396802048
1105	48-49	0.03618597987561856
1105	50-51	0.12345535656358209
1105	52-53	0.06225601059106367
1105	54-55	0.07528518616153335
1105	56-57	0.12038982647631968
1105	58-59	0.14411784506685876
1105	60-61	0.1513695132090369
1105	62-63	0.23865991178346846
1105	64-65	0.3210190039382681
1105	66-67	0.31774461670457654
1105	68-69	0.16997475731988843
1105	70-71	0.18794737640657644
1105	72-73	0.25823105166231386
1105	74-75	0.3138933389909262
1105	76-77	0.16861487748429482
1105	78-79	0.16292556030409955
1105	80-81	-0.015550644959635207
1105	82-83	0.10550444744144727
1105	84-85	0.30147381207249424
1105	86-87	0.5199520438140439
1105	88-89	0.3279176513015827
1105	90-91	0.3215125061087001
1105	92-93	0.4233065233331281
1105	94-95	0.5061928704160401
1105	96-97	0.33934456914575684
1105	98-99	0.41206125430421636
1105	100	0.4045516867810903
1106	1	-0.11553678788842348
1106	2	-0.13019723428673657
1106	3	-0.11111290821531128
1106	4	-0.09683138238434452
1106	5	-0.1577256067782642
1106	6	-0.14428073898621818
1106	7	-0.19339319197969473
1106	8	-0.15240960846501395
1106	9	-0.25444733820013
1106	10-11	-0.24400187043552535
1106	12-13	-0.2726622306817106
1106	14-15	-0.26307113772365653
1106	16-17	-0.26357274093451366
1106	18-19	-0.26230522406182644
1106	20-21	-0.2293543372445228
1106	22-23	-0.2740494645971623
1106	24-25	-0.2688834160348108
1106	26-27	-0.24671826357624127
1106	28-29	-0.27313495280185407
1106	30-31	-0.28624023099058604
1106	32-33	-0.3063020488181394
1106	34-35	-0.33750156096553496
1106	36-37	-0.4252248437302697
1106	38-39	-0.39769292171217785
1106	40-41	-0.23379847779480656
1106	42-43	-0.29845816795773317
1106	44-45	-0.34048316899652775
1106	46-47	-0.29477919867309765
1106	48-49	-0.4215675324926096
1106	50-51	-0.27715582174777253
1106	52-53	-0.32395496835479776
1106	54-55	-0.32420231412068645
1106	56-57	-0.2367970401943822
1106	58-59	-0.2132626143565055
1106	60-61	-0.16747348999504297
1106	62-63	-0.10905293446604958
1106	64-65	-0.018591426914486675
1106	66-67	-0.08636150999034697
1106	68-69	-0.10836213667473871
1106	70-71	-0.1882605976615821
1106	72-73	-0.08573767256019238
1106	74-75	-0.13501986819829526
1106	76-77	-0.3583640470957157
1106	78-79	-0.2285496594584231
1106	80-81	-0.2848085905118829
1106	82-83	-0.1352975935137195
1106	84-85	-0.0031443537132034294
1106	86-87	0.07599673896799786
1106	88-89	-0.07698679375584305
1106	90-91	0.1449745578139776
1106	92-93	0.19634038044056012
1106	94-95	0.41788205888287777
1106	96-97	0.20955203642169629
1106	98-99	0.2763049643304676
1106	100	0.13951086814731184
1107	1	-0.21105473907963557
1107	2	-0.19949031717014165
1107	3	-0.20632642092354558
1107	4	-0.188557922332798
1107	5	-0.2242453403518354
1107	6	-0.18872009517334476
1107	7	-0.28690103456862914
1107	8	-0.21505489901073815
1107	9	-0.2796410874774864
1107	10-11	-0.2841698803119499
1107	12-13	-0.2703487338992332
1107	14-15	-0.30355826195950897
1107	16-17	-0.278571069189276
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2207	70-71	0.8390872904794371
2207	72-73	0.9279095758210616
2207	74-75	0.8756848453237858
2207	76-77	0.8167217995578824
2207	78-79	0.8800538493188377
2207	80-81	0.8779072357800963
2207	82-83	1.017425894899496
2207	84-85	0.9680999182069705
2207	86-87	1.0005970317689226
2207	88-89	1.083775480221405
2207	90-91	1.1215220073510714
2207	92-93	0.7515479042531581
2207	94-95	0.581789565212425
2207	96-97	0.7401992525688339
2207	98-99	0.7316652985352761
2207	100	0.6296096893381709
2208	1	-0.7696052585169966
2208	2	-0.7119658575994237
2208	3	-0.47357244848547
2208	4	-0.3349699476455328
2208	5	-0.37811782419596796
2208	6	-0.3731780787029848
2208	7	-0.3629980155792083
2208	8	-0.37840898497064046
2208	9	-0.4315990689780804
2208	10-11	-0.4142414390663731
2208	12-13	-0.3692621868411976
2208	14-15	-0.5394710823878484
2208	16-17	-0.5279027862100278
2208	18-19	-0.4876625180875678
2208	20-21	-0.48880986379387537
2208	22-23	-0.48367841037751447
2208	24-25	-0.4546962168166786
2208	26-27	-0.46785216998011947
2208	28-29	-0.47303023190282545
2208	30-31	-0.5110069485409312
2208	32-33	-0.33048780049672644
2208	34-35	-0.29748627862279164
2208	36-37	-0.3199099695502099
2208	38-39	-0.3087636804224161
2208	40-41	-0.24286343184211034
2208	42-43	-0.14921611496440335
2208	44-45	-0.27526445122083487
2208	46-47	-0.4228360757804239
2208	48-49	-0.3001514382142112
2208	50-51	-0.10880325674191127
2208	52-53	-0.02271087085520307
2208	54-55	0.09389375678215117
2208	56-57	0.04602525215983988
2208	58-59	0.09171911067415905
2208	60-61	0.012582425166339561
2208	62-63	-0.03284559934638054
2208	64-65	-0.26657631768717494
2208	66-67	-0.0314574125826681
2208	68-69	0.026832262425333653
2208	70-71	-0.18130735386182195
2208	72-73	-0.07226346453171928
2208	74-75	-0.10335714380132188
2208	76-77	-0.16579340435166756
2208	78-79	-0.24116709942050463
2208	80-81	-0.3173186058204607
2208	82-83	-0.31942416121491135
2208	84-85	-0.44271128733824483
2208	86-87	-0.5524106863523528
2208	88-89	-0.5299290498756761
2208	90-91	-0.8027963310413462
2208	92-93	-1.6014119302269023
2208	94-95	-3.763596053145182
2208	96-97	-2.9189243788716386
2208	98-99	-2.4440942295192816
2208	100	-2.5494494776136065
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	420176.0
3	181610.0
4	177980.0
5	145273.0
6	135346.0
7	138866.0
8	146529.0
9	165492.0
10	193532.0
11	220941.0
12	248522.0
13	289935.0
14	354937.0
15	450208.0
16	537654.0
17	548498.0
18	514406.0
19	488951.0
20	500949.0
21	523370.0
22	549862.0
23	584003.0
24	619384.0
25	660395.0
26	715932.0
27	787536.0
28	886084.0
29	1012084.0
30	1163430.0
31	1344440.0
32	1580600.0
33	1923763.0
34	2461842.0
35	3316281.0
36	4126492.0
37	3386893.0
38	971409.0
39	27155.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.1829772574354	28.346114670134064	28.152238782535534	22.318669289895006
2	23.416084140729833	28.061578224553998	27.25951219704323	21.262825437672944
3	23.06347201314137	26.648835881102688	27.111376431745292	23.17631567401065
4	23.937633040003917	26.252675646189687	26.800443309500682	23.00924800430571
5	24.2468528192921	26.493496190184075	26.598558905146803	22.661092085377028
6	24.196878484795523	26.39448131472642	26.04265697426821	23.365983226209845
7	24.207812723803826	26.703683002317135	26.218575154161655	22.869929119717384
8	23.610695816233765	26.58401910577288	26.55706861351025	23.248216464483104
9	23.573334595254174	26.252066185122438	26.68641966617476	23.488179553448635
10-11	23.527324918373154	26.464692894404163	26.581418825048193	23.426563362174488
12-13	23.258798949900072	26.51112631223261	26.739508769796526	23.490565968070793
14-15	22.97619567205478	26.763190433746658	27.149434832342124	23.11117906185644
16-17	22.81958030717774	26.69778913157483	27.3328664281557	23.14976413309173
18-19	22.455492870202395	26.646969223524568	27.941188529290624	22.956349376982413
20-21	22.18538149833585	26.42516709862523	28.669975219087192	22.71947618395173
22-23	22.219046982705443	26.378646989296268	29.005966833176227	22.396339194822062
24-25	22.13614359722268	26.26072107323911	29.374168727250506	22.2289666022877
26-27	22.15802016619334	26.3119721149752	29.41592175296921	22.114085965862248
28-29	22.21083332176922	26.372888317086602	29.37683752109656	22.03944084004762
30-31	22.194465198665263	26.299950161664974	29.338287064348222	22.16729757532154
32-33	22.21391532487941	26.39502373873613	29.207126444049713	22.183934492334746
34-35	22.316191227554917	26.395893119316373	29.25422170515611	22.033693947972598
36-37	22.305481759233214	26.38627206046108	29.148576217301887	22.15966996300382
38-39	22.33721463097657	26.473185941469563	29.027399577040818	22.162199850513048
40-41	22.421499337786816	26.602996211581818	28.78843817995928	22.187066270672084
42-43	22.52775296512599	26.665894056535798	28.426137471495966	22.380215506842248
44-45	22.548486996836516	26.907262081029852	28.034828662082916	22.509422260050716
46-47	22.816048289990032	27.0532439613974	27.58868880743518	22.542018941177393
48-49	22.857311258633533	27.077018753640125	27.304423625391955	22.76124636233439
50-51	22.900293929633815	27.14336704434176	27.125902039353182	22.83043698667124
52-53	23.043080285415122	27.180518648726647	26.98189446867543	22.794506597182792
54-55	23.10047743512614	27.128978713870506	26.90009871098193	22.870445140021427
56-57	23.078048618464504	27.208580753807443	26.872811511186356	22.840559116541698
58-59	23.204889884175497	27.287791294139957	26.761087886121615	22.746230935562934
60-61	23.128965633420588	27.24659061101697	26.75422938078312	22.87021437477933
62-63	23.16445431048305	27.31661712194549	26.704254460432587	22.814674107138874
64-65	23.213752823972502	27.390774409049452	26.635650950421702	22.759821816556347
66-67	23.243413694614333	27.406868473090125	26.500499856109254	22.84921797618629
68-69	23.21317926516983	27.53258144983615	26.39748701881205	22.856752266181964
70-71	23.313810899734488	27.58517836771672	26.339467085746794	22.761543646802
72-73	23.268260866588612	27.654605794790843	26.154430062899632	22.922703275720917
74-75	23.369505333090167	27.701348181341835	26.091605523485256	22.837540962082738
76-77	22.94985964242048	28.09894064735318	25.905947313968973	23.04525239625736
78-79	23.15735600175623	28.058941210393666	25.82947376835853	22.954229019491567
80-81	23.127756025053515	28.10453137291484	25.650844178332477	23.11686842369917
82-83	23.140018018260296	28.19043563862837	25.571627754028686	23.097918589082646
84-85	23.114242187298682	28.232012796780342	25.478523508776245	23.175221507144727
86-87	23.00946462352996	28.40224436158091	25.282690563151604	23.305600451737522
88-89	23.23011646666332	28.34677928157932	25.14532833207791	23.277775919679446
90-91	22.993317173781712	28.439236015733876	25.14302347775911	23.424423332725294
92-93	22.985339133658027	28.561845821759942	25.023103971649302	23.429711072932736
94-95	23.033446768464945	28.531359260906257	24.870923895484434	23.564270075144364
96-97	22.955482527048368	28.65442251556737	24.620041000573252	23.77005395681101
98-99	22.92471226610275	28.696573199252384	24.564708403968865	23.814006130676002
100	23.68243619646118	28.745199351914625	23.905399005828883	23.66696544579531
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	898.0
1	709.0
2	507.5
3	477.5
4	508.5
5	670.0
6	842.0
7	980.0
8	1174.0
9	1526.5
10	2116.5
11	2909.0
12	4028.0
13	5817.5
14	9074.0
15	12767.0
16	17683.5
17	22002.0
18	25104.0
19	30124.0
20	36900.0
21	45995.0
22	58519.5
23	74311.5
24	92868.5
25	113566.5
26	138666.5
27	170890.0
28	207295.0
29	244156.5
30	281337.5
31	326518.5
32	385254.0
33	450411.0
34	520324.5
35	605509.5
36	707197.5
37	824953.0
38	935291.0
39	1016443.5
40	1081994.0
41	1173428.0
42	1321605.0
43	1451676.5
44	1528843.5
45	1584786.0
46	1570815.5
47	1488891.0
48	1405808.0
49	1356900.5
50	1308401.5
51	1212586.5
52	1082452.0
53	944726.0
54	831693.0
55	746454.0
56	657533.0
57	573622.0
58	513000.0
59	460294.5
60	404465.0
61	350583.0
62	306530.5
63	270588.0
64	237955.5
65	207040.0
66	178972.5
67	152968.5
68	128749.5
69	108022.5
70	90219.5
71	74870.5
72	62254.5
73	51871.0
74	42791.5
75	34605.5
76	28008.5
77	22653.0
78	18219.0
79	14626.5
80	11473.5
81	8836.5
82	6778.0
83	5003.5
84	3494.5
85	2431.0
86	1714.0
87	1196.5
88	821.0
89	551.0
90	368.5
91	260.0
92	178.5
93	116.0
94	71.5
95	38.5
96	20.0
97	14.5
98	7.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05893708331743627
2	0.006350620723946148
3	1.2922774728960185E-4
4	0.002052259701003915
5	0.018101115173922086
6	0.0075075167473006785
7	0.004907577545878927
8	0.0013107385796516757
9	1.2922774728960185E-4
10-11	8.292113784416119E-4
12-13	7.199831634706388E-4
14-15	2.9076243140160413E-4
16-17	7.015220567149814E-4
18-19	0.0013338149630962475
20-21	0.001055359936198415
22-23	0.003152233978528502
24-25	0.003918369908888285
26-27	0.0031445418507136443
28-29	0.0024399429428727205
30-31	0.0020845666378263156
32-33	0.005115264996880073
34-35	0.00355376305046405
36-37	0.001987645827359114
38-39	0.002872240526067698
40-41	0.0020061069341147713
42-43	0.0016584227568832237
44-45	0.0026491688194368378
46-47	0.0023814827714798053
48-49	0.0058337097347877405
50-51	0.004569123922025208
52-53	0.0028937784839492982
54-55	9.953613392425285E-4
56-57	5.092188613435502E-4
58-59	0.0021091814468338584
60-61	0.001338430239785162
62-63	0.0015168876050898502
64-65	0.003407612621981763
66-67	0.0012522784082587607
68-69	0.0017122676515872247
70-71	0.0011445886188507593
72-73	0.002976853464349757
74-75	0.004606046135536523
76-77	0.002806088226859926
78-79	0.005018344186412872
80-81	0.0015030417750231072
82-83	0.004636814646795952
84-85	0.0012230483225623033
86-87	0.0014491968803191065
88-89	0.0011907413857399026
90-91	1.9845689762331713E-4
92-93	0.0012168946203104172
94-95	0.005279876532118018
96-97	0.0029860840177275854
98-99	0.0018214958665581976
100	0.0022184096618048316
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.250076E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.58324404685241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.99322083088825	28.56071764393621
2	12.626135772736932	9.49062284230931
3	4.52999539303305	5.107557671624345
4	2.121921357788247	3.1899475295193644
5	1.1788335582428553	2.215219465503031
6	0.7107504137960025	1.6027383754857893
7	0.46608045501945766	1.2261770840525006
8	0.35073266632268113	1.0545337114886861
9	0.2524304481951114	0.8538439625435885
>10	1.4335972022836234	9.756530067739554
>50	0.11795627914903727	3.1211952749861487
>100	0.15345007237888317	14.34417853121214
>500	0.054519163697401576	13.950564465023058
>1k	0.010245349572539482	4.8849839717591905
>5k	4.094902996930864E-5	0.10747126549480623
>10k+	9.008786585877175E-5	0.533718137322401
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.153702251885802E-6
2	0.0	0.0	0.0	0.0	9.230553377828703E-6
3	0.0	0.0	0.0	0.0	9.230553377828703E-6
4	0.0	0.0	0.0	0.0	1.2307404503771605E-5
5	0.0	0.0	0.0	0.0	1.2307404503771605E-5
6	0.0	0.0	0.0	0.0	1.2307404503771605E-5
7	0.0	0.0	0.0	0.0	1.2307404503771605E-5
8	0.0	0.0	0.0	0.0	1.5384255629714506E-5
9	3.076851125942901E-6	0.0	0.0	0.0	1.8461106755657406E-5
10-11	3.538378794834336E-5	3.076851125942901E-6	0.0	0.0	2.1537957881600306E-5
12-13	1.7999579086765973E-4	6.153702251885802E-6	0.0	3.076851125942901E-6	2.769166013348611E-5
14-15	5.999859695588656E-4	9.230553377828703E-6	0.0	3.076851125942901E-6	2.9230085696457563E-5
16-17	0.0013415070909111048	9.230553377828703E-6	0.0	3.076851125942901E-6	3.692221351131481E-5
18-19	0.002626092435992266	9.230553377828703E-6	0.0	3.076851125942901E-6	4.615276688914351E-5
20-21	0.004290668895127376	9.230553377828703E-6	0.0	3.076851125942901E-6	4.922961801508642E-5
22-23	0.006222931402219517	1.0768978940800153E-5	0.0	6.153702251885802E-6	5.538332026697222E-5
24-25	0.008467494298594863	1.2307404503771605E-5	0.0	6.153702251885802E-6	6.307544808182948E-5
26-27	0.011025896009816386	1.2307404503771605E-5	0.0	6.153702251885802E-6	6.769072477074382E-5
28-29	0.01358583614660088	1.5384255629714506E-5	0.0	6.153702251885802E-6	7.076757589668672E-5
30-31	0.0159934721526512	1.5384255629714506E-5	0.0	6.153702251885802E-6	8.615183152640122E-5
32-33	0.018079577216040487	1.5384255629714506E-5	0.0	7.692127814857253E-6	8.922868265234412E-5
34-35	0.02009645312909606	1.5384255629714506E-5	0.0	9.230553377828703E-6	9.538238490422994E-5
36-37	0.022065637849699515	1.5384255629714506E-5	0.0	9.230553377828703E-6	1.0768978940800154E-4
38-39	0.0240009772079176	1.5384255629714506E-5	0.0	1.0768978940800153E-5	1.1230506609691588E-4
40-41	0.025965546651832143	1.5384255629714506E-5	0.0	1.2307404503771605E-5	1.1999719391177314E-4
42-43	0.02806549754528817	1.5384255629714506E-5	0.0	1.2307404503771605E-5	1.3076617285257328E-4
44-45	0.03061620712869484	1.9999532318628856E-5	0.0	1.2307404503771605E-5	1.39996726230402E-4
46-47	0.03362382910430402	2.1537957881600306E-5	0.0	1.2307404503771605E-5	1.4307357735634488E-4
48-49	0.03731912730656145	2.461480900754321E-5	0.0	1.2307404503771605E-5	1.461504284822878E-4
50-51	0.04170210173546711	2.461480900754321E-5	0.0	1.2307404503771605E-5	1.5076570517120216E-4
52-53	0.046831212562413925	3.076851125942901E-5	3.076851125942901E-6	1.2307404503771605E-5	1.5230413073417362E-4
54-55	0.053315676310338594	3.692221351131481E-5	4.6152766889143515E-6	1.8461106755657406E-5	1.5384255629714505E-4
56-57	0.06160625166919174	3.846063907428626E-5	7.692127814857253E-6	1.8461106755657406E-5	1.5384255629714505E-4
58-59	0.07177216778930709	4.922961801508642E-5	9.230553377828703E-6	1.9999532318628856E-5	1.553809818601165E-4
60-61	0.08404880378181925	4.922961801508642E-5	9.230553377828703E-6	2.3076383444571756E-5	1.5999625854903085E-4
62-63	0.09900230025390175	4.922961801508642E-5	1.0768978940800153E-5	2.461480900754321E-5	1.6307310967497376E-4
64-65	0.11698187980834909	4.922961801508642E-5	1.2307404503771605E-5	2.769166013348611E-5	1.6307310967497376E-4
66-67	0.13907213246705613	5.538332026697222E-5	1.5384255629714506E-5	2.769166013348611E-5	1.6307310967497376E-4
68-69	0.16633764871959916	5.999859695588657E-5	1.8461106755657406E-5	2.769166013348611E-5	1.6922681192685954E-4
70-71	0.19986609543899897	6.153702251885803E-5	1.8461106755657406E-5	2.769166013348611E-5	1.7538051417874536E-4
72-73	0.23946055415319517	6.153702251885803E-5	1.9999532318628856E-5	3.076851125942901E-5	1.7538051417874536E-4
74-75	0.28731943499167406	6.307544808182948E-5	2.1537957881600306E-5	3.384536238537191E-5	1.7691893974171682E-4
76-77	0.34372580825802224	6.461387364480093E-5	2.1537957881600306E-5	3.384536238537191E-5	1.799957908676597E-4
78-79	0.4100042583619583	6.769072477074382E-5	2.1537957881600306E-5	3.384536238537191E-5	1.8153421643063116E-4
80-81	0.4870563026833834	6.922915033371528E-5	2.1537957881600306E-5	3.846063907428626E-5	1.8153421643063116E-4
82-83	0.5757326905586208	7.076757589668672E-5	2.1537957881600306E-5	4.307591576320061E-5	1.830726419936026E-4
84-85	0.6765672556580216	7.076757589668672E-5	2.1537957881600306E-5	4.307591576320061E-5	1.8922634424548842E-4
86-87	0.7912122670362169	7.076757589668672E-5	2.1537957881600306E-5	5.076804357805787E-5	1.9538004649737422E-4
88	0.8860838946535404	7.076757589668672E-5	2.461480900754321E-5	5.230646914102932E-5	1.9691847206034568E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	19160	0.0	44.258102	1
TCTCGAG	23190	0.0	36.71169	2
CTCGAGG	29735	0.0	28.5981	3
GATCCGG	32205	0.0	27.484564	1
TCGAGGG	35940	0.0	23.595312	4
ATCCGGC	40340	0.0	21.95491	2
TATCTCG	13205	0.0	20.576715	2
GCGCCGT	50685	0.0	17.431929	9
TCCGGCG	51130	0.0	17.403694	3
GGATCCG	21655	0.0	17.149662	2
ATATCTC	16675	0.0	16.725588	1
ATGGATC	21235	0.0	16.2347	1
CGAGGGC	52855	0.0	16.213142	5
CGGCGCG	55435	0.0	15.975831	5
AGGGCGC	55030	0.0	15.287386	7
TGGATCC	25575	0.0	15.208361	1
CGTCGAC	48930	0.0	14.719542	12-13
CCGGCGC	61315	0.0	14.512797	4
TCGACTT	51085	0.0	14.121648	14-15
GCCGTCG	51670	0.0	14.070804	10-11
>>END_MODULE