FastQCFastQC Report
Wed 25 May 2016
SRR491014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24177697
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259290.10724346491727478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA170000.054.582181
TCTCGAG211300.043.738462
CTCGAGG269900.034.223323
TCGAGGG315450.029.3562664
GATCCGG274050.029.0879151
ATCCGGC319100.024.9849552
TATCTCG76650.024.1637082
ATGGATC170350.020.5125371
CGAGGGC476600.019.7260935
TCCGGCG422150.019.0744933
GGATCCG178100.018.8450263
AGGGCGC491250.018.7706347
GCGCCGT453850.017.5934479
TGGATCC202850.017.3342912
CGGCGCG466500.017.0798175
GGGCGCG549800.016.7114018
CCGGCGC508100.015.7646934
GAGGGCG617750.015.1630326
ATATCTC128800.014.5324851
CGTCGAC441250.014.08575112-13
Produced by FastQC (version 0.11.5)