##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491014_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24177697
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.655133406626778	33.0	31.0	34.0	30.0	34.0
2	31.944045621880363	34.0	31.0	34.0	30.0	34.0
3	32.03553072072994	34.0	31.0	34.0	30.0	34.0
4	35.40139178681907	37.0	35.0	37.0	33.0	37.0
5	35.206186304675754	37.0	35.0	37.0	32.0	37.0
6	35.15470708397082	37.0	35.0	37.0	32.0	37.0
7	35.080176370809845	37.0	35.0	37.0	32.0	37.0
8	34.991360632900644	37.0	35.0	37.0	32.0	37.0
9	36.548635008537	39.0	37.0	39.0	32.0	39.0
10-11	36.39607688854733	39.0	37.0	39.0	32.0	39.0
12-13	36.16916997925816	39.0	37.0	39.0	32.0	39.0
14-15	37.497189972229364	40.0	38.0	41.0	32.0	41.0
16-17	37.421335683874275	40.0	38.0	41.0	32.0	41.0
18-19	37.299435529364104	40.0	38.0	41.0	31.5	41.0
20-21	37.152539693917085	40.0	38.0	41.0	31.0	41.0
22-23	36.95374863453703	40.0	38.0	41.0	31.0	41.0
24-25	36.74470829459067	40.0	37.0	41.0	30.0	41.0
26-27	36.39970608035993	40.0	37.0	41.0	30.0	41.0
28-29	36.06298676834274	40.0	36.0	41.0	28.5	41.0
30-31	35.751124248930736	39.0	35.5	41.0	27.0	41.0
32-33	35.51680211725707	39.0	35.0	41.0	26.5	41.0
34-35	35.21425338815355	39.0	35.0	41.0	25.0	41.0
36-37	34.90424588413032	39.0	35.0	40.0	24.0	41.0
38-39	34.510690865221775	39.0	35.0	40.0	22.0	41.0
40-41	34.012898933260686	38.0	34.0	40.0	16.5	41.0
42-43	33.53022789970443	38.0	33.0	40.0	11.0	41.0
44-45	33.017955514952476	38.0	33.0	40.0	4.5	41.0
46-47	32.46373136779735	38.0	32.0	40.0	2.0	41.0
48-49	31.89880003872991	37.5	31.0	40.0	2.0	41.0
50-51	31.362619297445907	37.0	30.5	40.0	2.0	41.0
52-53	30.77484704188327	37.0	29.5	39.5	2.0	41.0
54-55	30.147004303180736	36.0	28.0	39.0	2.0	40.0
56-57	29.592289373135912	35.5	27.5	39.0	2.0	40.0
58-59	28.964739073369977	35.0	26.0	38.5	2.0	40.0
60-61	28.344562035829963	34.5	25.5	38.0	2.0	40.0
62-63	27.67856859981329	34.0	22.5	38.0	2.0	40.0
64-65	26.966306116748836	33.5	21.0	37.5	2.0	39.5
66-67	26.26098271477221	33.0	17.5	36.5	2.0	39.0
68-69	25.534197818758336	32.0	10.5	36.0	2.0	39.0
70-71	24.81164186150567	31.5	7.0	35.5	2.0	38.5
72-73	24.00280221064893	31.0	2.0	35.0	2.0	37.5
74-75	23.217106637575945	30.0	2.0	34.5	2.0	37.0
76-77	22.432542334367085	29.0	2.0	34.0	2.0	36.0
78-79	22.560378558801528	30.0	2.0	34.0	2.0	36.0
80-81	21.898896780781065	29.5	2.0	34.0	2.0	35.5
82-83	21.119695953671684	29.0	2.0	34.0	2.0	35.0
84-85	20.458361129267193	27.5	2.0	33.5	2.0	35.0
86-87	19.57809649943086	26.5	2.0	33.0	2.0	35.0
88-89	18.632792631159205	25.0	2.0	32.0	2.0	34.0
90-91	17.893273892050182	23.5	2.0	32.0	2.0	34.0
92-93	17.025848677812448	19.5	2.0	31.5	2.0	34.0
94-95	16.037387866180968	12.0	2.0	31.0	2.0	34.0
96-97	15.153370211397718	2.0	2.0	31.0	2.0	34.0
98-99	14.323359044494602	2.0	2.0	31.0	2.0	34.0
100	13.588648207478156	2.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0941808331648133
1101	2	0.09751595027186966
1101	3	0.11247450693099381
1101	4	0.1277977971436428
1101	5	0.15717303893499235
1101	6	0.12976211226350642
1101	7	0.15682023818939683
1101	8	0.17725238645205366
1101	9	0.12700999678326497
1101	10-11	0.15636711987644958
1101	12-13	0.14216040240171424
1101	14-15	0.18063207852841145
1101	16-17	0.2032714085228946
1101	18-19	0.20202504325888526
1101	20-21	0.17008132375151774
1101	22-23	0.17878846698955186
1101	24-25	0.12581105844346752
1101	26-27	0.11852202248998367
1101	28-29	0.15602535296805797
1101	30-31	0.123961240974225
1101	32-33	0.11001119636090095
1101	34-35	0.10852499052666076
1101	36-37	0.05969602520305273
1101	38-39	0.033344231280835857
1101	40-41	0.05651139864868071
1101	42-43	-0.06857934038350066
1101	44-45	-0.10947956613323839
1101	46-47	0.0018158316277308018
1101	48-49	-0.14645204110646404
1101	50-51	-0.20732345979719113
1101	52-53	-0.21180294179899306
1101	54-55	-0.2140764660233927
1101	56-57	-0.2275606234960179
1101	58-59	-0.24264908900269333
1101	60-61	-0.33783864708059497
1101	62-63	-0.27685594783275747
1101	64-65	-0.27945123506270164
1101	66-67	-0.3646628164361907
1101	68-69	-0.5185730116726823
1101	70-71	-0.6228806835620766
1101	72-73	-0.5521109544129956
1101	74-75	-0.5008217381910569
1101	76-77	-0.5890278973378464
1101	78-79	-0.5605813433933058
1101	80-81	-0.6107548659732274
1101	82-83	-0.5964652960201704
1101	84-85	-0.59149890641919
1101	86-87	-0.30069379878452196
1101	88-89	-0.43779581784987087
1101	90-91	-0.43008108784534116
1101	92-93	-0.3758834657623318
1101	94-95	-0.24722967695296205
1101	96-97	-0.34996547231069997
1101	98-99	-0.16662629684539532
1101	100	-0.16390613148264954
1102	1	0.12614063490183725
1102	2	0.1378827411850878
1102	3	0.09490196194980172
1102	4	0.13753736616124002
1102	5	0.12735339246366806
1102	6	0.15629905878599004
1102	7	0.15071424772865782
1102	8	0.1636427751881726
1102	9	0.16859835771580123
1102	10-11	0.1565398307239505
1102	12-13	0.14303850713649524
1102	14-15	0.1336780688631123
1102	16-17	0.15252679240855116
1102	18-19	0.11977335368418096
1102	20-21	0.101068816706686
1102	22-23	0.12367280890084231
1102	24-25	0.04992467139840784
1102	26-27	0.06785230266326892
1102	28-29	0.11137948349310989
1102	30-31	0.020436969046016884
1102	32-33	0.0080154887227053
1102	34-35	0.04616472497330193
1102	36-37	0.032862309865670625
1102	38-39	0.014431796067711389
1102	40-41	0.005951590008251628
1102	42-43	-0.04595605837126726
1102	44-45	-0.05624288329651961
1102	46-47	0.020705130092686375
1102	48-49	-0.0994584485870682
1102	50-51	-0.15292977571981936
1102	52-53	-0.2218413615556365
1102	54-55	-0.1886592719142719
1102	56-57	-0.2295213533227276
1102	58-59	-0.3639663803888702
1102	60-61	-0.44019270139146727
1102	62-63	-0.26566387767956456
1102	64-65	-0.19359894418732893
1102	66-67	-0.3607976899473293
1102	68-69	-0.42892859889751733
1102	70-71	-0.47895226260651924
1102	72-73	-0.4953997509293835
1102	74-75	-0.5647268008452819
1102	76-77	-0.6576916386862308
1102	78-79	-0.7158349832189081
1102	80-81	-0.6848048707984482
1102	82-83	-0.6617889646616177
1102	84-85	-0.6438136753551476
1102	86-87	-0.4004747972949829
1102	88-89	-0.49239271944445306
1102	90-91	-0.486260466845831
1102	92-93	-0.39040136399149006
1102	94-95	-0.24484154104136735
1102	96-97	-0.4096224037393519
1102	98-99	-0.309176156839742
1102	100	-0.2617921184119627
1103	1	0.019684849666003146
1103	2	0.023961854459585652
1103	3	0.02037202420999762
1103	4	-0.05966919030829132
1103	5	0.008879230327814014
1103	6	0.018228121397072528
1103	7	0.028098700022887613
1103	8	0.03886411161361991
1103	9	0.04894479690732112
1103	10-11	0.03648441770104682
1103	12-13	-0.05358603089661784
1103	14-15	-0.06333282978167887
1103	16-17	-0.002646393201921171
1103	18-19	0.03179921663887342
1103	20-21	-0.045538592685971935
1103	22-23	-0.08141840149674096
1103	24-25	-0.048812912632051564
1103	26-27	-0.04257760112446363
1103	28-29	-0.002119776480810742
1103	30-31	-0.06699957004923363
1103	32-33	-0.05828727042806747
1103	34-35	-0.013494493892558523
1103	36-37	-0.009865501179113778
1103	38-39	-0.13444988867261998
1103	40-41	-0.08232941727553111
1103	42-43	-0.16290690031350863
1103	44-45	-0.13610432655256233
1103	46-47	-0.16096170897820627
1103	48-49	-0.23002597572006422
1103	50-51	-0.3383605969976564
1103	52-53	-0.49777057263202096
1103	54-55	-0.35597015600367143
1103	56-57	-0.3178999941105225
1103	58-59	-0.38195902620949695
1103	60-61	-0.338130977962642
1103	62-63	-0.21884157991483733
1103	64-65	-0.21138837320236803
1103	66-67	-0.2953473011976122
1103	68-69	-0.308396868716855
1103	70-71	-0.3427264812829627
1103	72-73	-0.3053666675627582
1103	74-75	-0.31701038990705044
1103	76-77	-0.48270234574073
1103	78-79	-0.41483166605789634
1103	80-81	-0.48289809887459256
1103	82-83	-0.3782409425880857
1103	84-85	-0.23025999894094085
1103	86-87	0.05477071165083913
1103	88-89	-0.07546126178580437
1103	90-91	-0.14922601310349748
1103	92-93	-0.19510717195410265
1103	94-95	-0.38253759566614676
1103	96-97	-0.5702063098655401
1103	98-99	-0.5966718430032003
1103	100	-0.5719730249331736
1104	1	0.015044430758564431
1104	2	0.009977611002295106
1104	3	0.010599031520655444
1104	4	0.02796329128194941
1104	5	-0.014349793757446605
1104	6	0.026909636664100844
1104	7	-0.0012573517129865763
1104	8	-0.02349683452735718
1104	9	-0.040444224881952096
1104	10-11	-0.04558346547648995
1104	12-13	-0.028046667117507695
1104	14-15	0.03686678908024987
1104	16-17	0.046344764551989215
1104	18-19	0.030505379004530653
1104	20-21	0.05236796260982857
1104	22-23	0.07302947770869395
1104	24-25	-0.025644359266600247
1104	26-27	0.04291374976306628
1104	28-29	0.06750885486860625
1104	30-31	0.01762014350168073
1104	32-33	0.05811891063409291
1104	34-35	0.10979046183046393
1104	36-37	0.09930601186393773
1104	38-39	0.12535103632771438
1104	40-41	0.17778763913803175
1104	42-43	0.14981308330514054
1104	44-45	0.11654769353257421
1104	46-47	0.14496269984244492
1104	48-49	0.1179289719385217
1104	50-51	0.12924356282547578
1104	52-53	0.06387802278047872
1104	54-55	0.16545764679955255
1104	56-57	0.09665420863936092
1104	58-59	0.016290539223543732
1104	60-61	0.08235496081616134
1104	62-63	0.12598317636822998
1104	64-65	0.21182763812802818
1104	66-67	0.14992400459892252
1104	68-69	0.01236827173384114
1104	70-71	-0.045466919691094176
1104	72-73	0.1433873321491106
1104	74-75	0.17801975241786394
1104	76-77	0.022923410640824926
1104	78-79	0.06263974751889734
1104	80-81	-0.03896549305284225
1104	82-83	0.04223818082108011
1104	84-85	0.24241911908627145
1104	86-87	0.46478873831028267
1104	88-89	0.278843552677575
1104	90-91	0.23901968533927587
1104	92-93	0.24795509591746523
1104	94-95	0.4170336481839705
1104	96-97	0.010504943693229407
1104	98-99	0.10391024479580757
1104	100	0.1858137845024661
1105	1	-0.004084785301994742
1105	2	5.742078000245954E-4
1105	3	-0.011656350659045245
1105	4	0.007723195122039783
1105	5	-0.006745143557495226
1105	6	-0.005028613083666755
1105	7	-0.04285129315092462
1105	8	-0.023063635664271942
1105	9	-0.07086473553970762
1105	10-11	-0.01129921863947203
1105	12-13	-0.0037963820787254576
1105	14-15	0.011186468834679886
1105	16-17	0.0069610669055322205
1105	18-19	-0.007195239001376308
1105	20-21	0.02979561163245137
1105	22-23	0.009588113361651551
1105	24-25	-0.0031895307949127982
1105	26-27	0.025734509558404284
1105	28-29	0.0614374025189548
1105	30-31	0.04048338681511865
1105	32-33	0.02525176138735219
1105	34-35	-0.010754911314222682
1105	36-37	0.05605002340494991
1105	38-39	0.0365865915554906
1105	40-41	0.12766290396920965
1105	42-43	0.14557726717197994
1105	44-45	0.09542537927929118
1105	46-47	0.15274072445007647
1105	48-49	7.459706592349846E-4
1105	50-51	0.09781249885695331
1105	52-53	0.01586272607052308
1105	54-55	0.061913909505989295
1105	56-57	0.08478991520027179
1105	58-59	0.10774507800119792
1105	60-61	0.10055317527308105
1105	62-63	0.18582906496029494
1105	64-65	0.2891862009711268
1105	66-67	0.27876607612386195
1105	68-69	0.1286881712039758
1105	70-71	0.1457721106076093
1105	72-73	0.23397845596101519
1105	74-75	0.24658856555307906
1105	76-77	0.09590099830051813
1105	78-79	0.06133492615451175
1105	80-81	-0.09879757157722935
1105	82-83	0.0689682144110435
1105	84-85	0.24949941309457557
1105	86-87	0.4764669485903177
1105	88-89	0.23804991254222685
1105	90-91	0.2482201193674065
1105	92-93	0.36682129196077895
1105	94-95	0.430890560321739
1105	96-97	0.32597328228885303
1105	98-99	0.3857241002542633
1105	100	0.3745047538073454
1106	1	-0.1304421061936729
1106	2	-0.15676067326056042
1106	3	-0.1322065783746993
1106	4	-0.12978641694604676
1106	5	-0.17189320651309714
1106	6	-0.1611020912171881
1106	7	-0.20692687417123068
1106	8	-0.17711244664047854
1106	9	-0.2540164576156627
1106	10-11	-0.256702059930042
1106	12-13	-0.311649379343784
1106	14-15	-0.2902663543903543
1106	16-17	-0.2916505790767232
1106	18-19	-0.30680848139377304
1106	20-21	-0.2432355610033312
1106	22-23	-0.2955405596296643
1106	24-25	-0.2853689119152776
1106	26-27	-0.2802131602103799
1106	28-29	-0.2777717935055364
1106	30-31	-0.2794207765801957
1106	32-33	-0.26195985401291466
1106	34-35	-0.32360142254278657
1106	36-37	-0.39839323395436566
1106	38-39	-0.4024751444303263
1106	40-41	-0.24579605138309546
1106	42-43	-0.30067853535634725
1106	44-45	-0.3479470742294737
1106	46-47	-0.29718828414597454
1106	48-49	-0.38011838449407165
1106	50-51	-0.26087967358109765
1106	52-53	-0.33128451743632326
1106	54-55	-0.35007690456168206
1106	56-57	-0.25123916710153793
1106	58-59	-0.22125499299830764
1106	60-61	-0.1735248605581674
1106	62-63	-0.14964693733671552
1106	64-65	-0.08231925027826037
1106	66-67	-0.13188462105404497
1106	68-69	-0.1787678286904466
1106	70-71	-0.2006929680074876
1106	72-73	-0.13077725394837358
1106	74-75	-0.1813056211041122
1106	76-77	-0.4379206554744215
1106	78-79	-0.28188415284835955
1106	80-81	-0.3343676769285935
1106	82-83	-0.17122730353329985
1106	84-85	-0.03475710212811833
1106	86-87	0.06186486578957329
1106	88-89	-0.14153478003945352
1106	90-91	0.07806810285417498
1106	92-93	0.1551703988175568
1106	94-95	0.3438443824122608
1106	96-97	0.14867553678824663
1106	98-99	0.2519511930063487
1106	100	0.15228035738724266
1107	1	-0.19238573877086296
1107	2	-0.18598992261881975
1107	3	-0.18072941657784725
1107	4	-0.16604121892829227
1107	5	-0.20465767945520952
1107	6	-0.17099086558704357
1107	7	-0.30003999474822507
1107	8	-0.2132816965646427
1107	9	-0.2746352980965199
1107	10-11	-0.2866239297295934
1107	12-13	-0.26951423210910974
1107	14-15	-0.28896476061771637
1107	16-17	-0.28269251630625547
1107	18-19	-0.30748473986184166
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2207	76-77	0.8273821870895084
2207	78-79	0.896388795513186
2207	80-81	0.9451857873593852
2207	82-83	1.0284831184356769
2207	84-85	0.9952761520996347
2207	86-87	1.024146524959555
2207	88-89	1.088859325228288
2207	90-91	1.1215411185933881
2207	92-93	0.7961260664112579
2207	94-95	0.6195205590318302
2207	96-97	0.7767661142517
2207	98-99	0.7936826897413578
2207	100	0.6560214706723446
2208	1	-0.7096934147253151
2208	2	-0.6600539543205564
2208	3	-0.425070742402589
2208	4	-0.2693012868029143
2208	5	-0.30568415211087796
2208	6	-0.29802149135787204
2208	7	-0.2879868354213144
2208	8	-0.2861051462744797
2208	9	-0.3461718985626234
2208	10-11	-0.33134282562807726
2208	12-13	-0.2909883033426439
2208	14-15	-0.43611442147266644
2208	16-17	-0.4412311462285601
2208	18-19	-0.3990218795289735
2208	20-21	-0.3975570059752087
2208	22-23	-0.3734417046640033
2208	24-25	-0.3396599210275113
2208	26-27	-0.33285714420744483
2208	28-29	-0.3610187258302062
2208	30-31	-0.4237364974185027
2208	32-33	-0.23347118969311254
2208	34-35	-0.18127022470077492
2208	36-37	-0.18396359017835806
2208	38-39	-0.14932507607565526
2208	40-41	-0.11455918230684858
2208	42-43	-0.006566865238504249
2208	44-45	-0.1626874577657773
2208	46-47	-0.29184231123172566
2208	48-49	-0.20043920828763007
2208	50-51	-0.004481264770880955
2208	52-53	0.07853151633348432
2208	54-55	0.210805419145224
2208	56-57	0.16587445583401106
2208	58-59	0.24135211533506506
2208	60-61	0.15377388665611846
2208	62-63	0.1164854346437103
2208	64-65	-0.08562372165248178
2208	66-67	0.1254169973905377
2208	68-69	0.21995589824930661
2208	70-71	0.01396856367638577
2208	72-73	0.10164826082716871
2208	74-75	0.07659302807136115
2208	76-77	-0.025869289712488808
2208	78-79	-0.05115517111390844
2208	80-81	-0.1682294751015263
2208	82-83	-0.1473695440349445
2208	84-85	-0.2738357596437453
2208	86-87	-0.3432270308338765
2208	88-89	-0.2906486939693025
2208	90-91	-0.5551408282510799
2208	92-93	-1.3407257186270343
2208	94-95	-3.463944016411409
2208	96-97	-2.6469587273348694
2208	98-99	-2.142595840352712
2208	100	-2.2110900596382947
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	297181.0
3	135372.0
4	135898.0
5	112548.0
6	104735.0
7	107574.0
8	114459.0
9	130355.0
10	152531.0
11	174526.0
12	198049.0
13	232422.0
14	289386.0
15	371275.0
16	439790.0
17	451026.0
18	418770.0
19	390741.0
20	397543.0
21	411287.0
22	432427.0
23	456696.0
24	480999.0
25	511151.0
26	550637.0
27	602608.0
28	672965.0
29	768182.0
30	877996.0
31	1010819.0
32	1182145.0
33	1429782.0
34	1819944.0
35	2420125.0
36	2934349.0
37	2314679.0
38	625294.0
39	21421.0
40	10.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.173936010308484	27.994389198337714	26.916651437016792	21.915023354337006
2	23.85529593319177	27.975900910412534	27.22835527841595	20.940447877979746
3	23.56490998599452	26.285228666668708	27.337041656847006	22.812819690489768
4	24.593451866942814	26.17309253369292	26.799745579889	22.433710019475264
5	24.63607033986404	26.560091240445626	26.205926409467562	22.597912010222768
6	24.83341333541249	26.153573797316437	25.632422123094557	23.380590744176512
7	24.527360866996112	26.431393070956734	26.102471689714424	22.93877437233273
8	23.95291351435146	26.294250498025683	26.236303983527144	23.516532004095716
9	24.0580017770941	25.880385593766615	26.443388493177157	23.618224135962134
10-11	23.920238394733794	26.2108367277533	26.48683659912156	23.382088278391347
12-13	23.618297006072527	26.170033691967344	26.731016384481602	23.480652917478526
14-15	23.26671567198587	26.43885018176396	27.221881285182935	23.07255286106723
16-17	23.133649688553255	26.33406870504782	27.42241429281857	23.109867313580352
18-19	22.729028118246294	26.374660076067517	28.13988834562825	22.756423460057935
20-21	22.361218048785805	26.02932407685003	28.977368197780713	22.63208967658345
22-23	22.4289713693325	25.94703048564198	29.378325867331274	22.245672277694243
24-25	22.34514447021226	25.80640891668027	29.86655533679901	21.98189127630846
26-27	22.357379308544882	25.844803425640116	29.887530780583116	21.910286485231886
28-29	22.47014323796592	25.91508066637067	29.82302355846862	21.791752537194785
30-31	22.431949942302094	25.814735403517535	29.78479060485882	21.96852404932155
32-33	22.420412597322805	25.952180757993858	29.67803406795574	21.9493725767276
34-35	22.563128959632696	25.92409620108335	29.71485422674171	21.797920612542242
36-37	22.560937703768328	25.90475567695331	29.597084832520192	21.93722178675817
38-39	22.56795370551442	25.99120248261905	29.46590501867722	21.97493879318931
40-41	22.671811059179632	26.161773850047172	29.154093472985476	22.01232161778772
42-43	22.818926258659424	26.22383756393719	28.737871313844465	22.21936486355892
44-45	22.85113274498069	26.46719976043456	28.278421200879677	22.403246293705077
46-47	23.15896630009015	26.631355533049017	27.754429421158715	22.455248745702118
48-49	23.22948946242232	26.63684146511934	27.420449451954216	22.71321962050413
50-51	23.250244631472754	26.706433650040136	27.190058929738985	22.853262788748122
52-53	23.43657175639124	26.7438886762216	27.02308532742525	22.796454239961914
54-55	23.51476822096786	26.677389864596773	26.9498064914847	22.858035422950664
56-57	23.458235140657617	26.754051297704684	26.929281730594145	22.858431831043557
58-59	23.615978510088265	26.826570942708322	26.807002999259883	22.75044754794353
60-61	23.584157946129864	26.768967466121385	26.75263407028493	22.894240517463817
62-63	23.58151465473356	26.855111317805143	26.72910751028934	22.834266517171955
64-65	23.65965878548107	26.921215421851137	26.64007790997085	22.779047882696943
66-67	23.711921453503283	26.907790683109162	26.495655015760434	22.884632847627124
68-69	23.645303122929562	27.04687294778736	26.412516344544013	22.89530758473907
70-71	23.745801027720265	27.098462460552415	26.34706323052057	22.808673281206758
72-73	23.72990237859449	27.11571330267384	26.145114047020463	23.00927027171121
74-75	23.806202849597515	27.19682946787858	26.09418962440493	22.902778058118976
76-77	23.346584807122913	27.609066150587836	25.925828420471614	23.118520621817634
78-79	23.61509061346242	27.533548786052243	25.8327126119669	23.018647988518435
80-81	23.51371092791914	27.640266149048927	25.653483434757128	23.192539488274807
82-83	23.568340610269995	27.69099270210664	25.584840698784454	23.155825988838906
84-85	23.515001899288727	27.729470641099287	25.48458747051801	23.270939989093982
86-87	23.405112412916523	27.91157842813728	25.28336271712046	23.399946441825737
88-89	23.6094328398261	27.882465140820443	25.16400068146029	23.344101337893168
90-91	23.375029761473307	27.971080151067618	25.13750870871427	23.516381378744803
92-93	23.336786490667798	28.13154918435056	25.03333641403525	23.498327910946387
94-95	23.39686883871748	28.092824873085583	24.890844613759032	23.619461674437904
96-97	23.31507603343277	28.23626020370769	24.582038645330464	23.866625117529075
98-99	23.255257309153844	28.311235820539444	24.555369023593382	23.87813784671333
100	24.105682618194187	28.326281850122903	23.85637317631256	23.71166235537035
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	195.0
1	163.0
2	120.5
3	119.0
4	141.5
5	181.0
6	212.0
7	259.0
8	341.5
9	487.5
10	750.5
11	1205.0
12	1978.0
13	3255.0
14	5253.5
15	8211.5
16	14103.5
17	20968.5
18	23706.0
19	26419.0
20	48383.0
21	58955.0
22	52911.0
23	58044.0
24	65117.0
25	75423.5
26	89750.5
27	109440.5
28	133243.0
29	157318.0
30	180245.5
31	209000.5
32	247689.5
33	292732.5
34	346091.0
35	409632.5
36	481509.5
37	571025.5
38	663186.5
39	732538.5
40	789034.0
41	862569.5
42	975886.0
43	1082022.5
44	1150876.5
45	1201804.5
46	1198469.5
47	1144526.0
48	1085665.0
49	1048604.5
50	1010978.0
51	932651.0
52	828198.0
53	719653.0
54	630847.5
55	564882.5
56	496302.5
57	431567.5
58	386869.5
59	348807.0
60	307434.5
61	266773.0
62	233312.0
63	206537.0
64	182352.0
65	159743.0
66	138696.5
67	118853.5
68	100681.5
69	84763.5
70	71007.5
71	59390.5
72	50130.0
73	42512.5
74	35658.0
75	29345.5
76	23804.0
77	19261.5
78	15678.5
79	12757.5
80	10067.0
81	7802.5
82	5937.0
83	4329.0
84	3161.5
85	2250.0
86	1557.5
87	1123.5
88	790.5
89	518.5
90	361.5
91	262.5
92	171.0
93	108.0
94	58.5
95	34.0
96	25.5
97	13.0
98	6.5
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05916609840879385
2	0.006261969450605655
3	6.20406484538209E-5
4	0.0015013836925824656
5	0.017400333869681633
6	0.00513696569197637
7	0.003288154368052507
8	9.471538997283323E-4
9	9.926503752611342E-5
10-11	6.183384629230816E-4
12-13	5.12869360551586E-4
14-15	2.0473413989760894E-4
16-17	5.314815550877323E-4
18-19	9.471538997283323E-4
20-21	7.961883218240347E-4
22-23	0.0022541435604888256
24-25	0.003707962755923362
26-27	0.0030048354067800584
28-29	0.002285163884715736
30-31	0.001970824599216377
32-33	0.004942571660154398
34-35	0.0033812153407332384
36-37	0.0017536823296280036
38-39	0.0026842920564353174
40-41	0.0017929747403154237
42-43	0.00147449941158581
44-45	0.0024278573761595244
46-47	0.002173490717498858
48-49	0.005734623938748178
50-51	0.004237376289395967
52-53	0.0026967001861260814
54-55	8.60296991892983E-4
56-57	4.3221651756161886E-4
58-59	0.0016502812488716357
60-61	9.864463104157521E-4
62-63	0.0010464189372544456
64-65	0.002318252230557774
66-67	9.161335755014218E-4
68-69	0.001184976385467979
70-71	8.416847973568368E-4
72-73	0.0021507424797324575
74-75	0.0033460589732760733
76-77	0.0021176541338904196
78-79	0.003530112897022409
80-81	0.0011043235424780119
82-83	0.0035445890483283
84-85	9.306097268073133E-4
86-87	0.0010278067427182994
88-89	8.520249054324735E-4
90-91	1.9232601020684477E-4
92-93	0.0012676972500730735
94-95	0.005242434794347865
96-97	0.002899366304408563
98-99	0.00177229452416415
100	0.002125926220350929
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.4177697E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.89160489628036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4097111043844	29.71069793198821
2	12.032308745908942	9.118469779800806
3	3.9405985258454472	4.479468071886016
4	1.6905920934301524	2.562369905801233
5	0.886114711615456	1.6788154272657136
6	0.5652769801170447	1.2851551192534554
7	0.3717704040769896	0.9860884084391051
8	0.2736022642810972	0.8293783117493613
9	0.20627893706082187	0.7034615983380013
>10	1.2476324704192874	8.408800489435588
>50	0.11277242907290641	3.011610720721105
>100	0.19954717211357964	18.935479447830687
>500	0.05686019772849025	14.178596826552095
>1k	0.00679179691624185	3.44378939343514
>5k	2.1871850647613483E-5	0.05469812852392838
>10k+	1.2029517856187411E-4	0.6131204389794669
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25929	0.10724346491727478	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	4.136043230254726E-6
3	0.0	4.136043230254726E-6	0.0	0.0	4.136043230254726E-6
4	0.0	1.2408129690764178E-5	0.0	0.0	4.136043230254726E-6
5	0.0	1.6544172921018904E-5	0.0	0.0	4.136043230254726E-6
6	0.0	2.4816259381528355E-5	0.0	0.0	4.136043230254726E-6
7	0.0	2.895230261178308E-5	0.0	0.0	4.136043230254726E-6
8	2.068021615127363E-5	3.308834584203781E-5	0.0	0.0	4.136043230254726E-6
9	5.790460522356616E-5	3.308834584203781E-5	0.0	0.0	4.136043230254726E-6
10-11	2.812509396573214E-4	3.308834584203781E-5	0.0	0.0	4.136043230254726E-6
12-13	0.0012780373581487104	3.308834584203781E-5	0.0	0.0	4.136043230254726E-6
14-15	0.003970601501044537	3.308834584203781E-5	0.0	0.0	4.136043230254726E-6
16-17	0.009155131690168836	3.308834584203781E-5	0.0	0.0	4.136043230254726E-6
18-19	0.017830482365628127	4.3428453917674625E-5	0.0	0.0	6.204064845382089E-6
20-21	0.029417607475186743	4.5496475532801985E-5	0.0	0.0	8.272086460509452E-6
22-23	0.04241305530464709	4.5496475532801985E-5	0.0	0.0	8.272086460509452E-6
24-25	0.056531438871121595	4.5496475532801985E-5	0.0	0.0	8.272086460509452E-6
26-27	0.07211191371949115	4.7564497147929344E-5	0.0	0.0	1.0340108075636815E-5
28-29	0.08870985520250337	5.170054037818408E-5	0.0	0.0	2.2748237766400992E-5
30-31	0.10404009943544251	5.3768561993311436E-5	0.0	4.136043230254726E-6	2.4816259381528355E-5
32-33	0.11778210306796383	5.3768561993311436E-5	0.0	4.136043230254726E-6	2.6884280996655718E-5
34-35	0.13040323898508616	5.3768561993311436E-5	0.0	4.136043230254726E-6	2.895230261178308E-5
36-37	0.14239569633120971	5.3768561993311436E-5	0.0	4.136043230254726E-6	2.895230261178308E-5
38-39	0.15330244232939144	5.3768561993311436E-5	0.0	4.136043230254726E-6	3.515636745716517E-5
40-41	0.16338818374636757	5.3768561993311436E-5	0.0	4.136043230254726E-6	3.722438907229253E-5
42-43	0.1720966227676689	5.5836583608438796E-5	0.0	4.136043230254726E-6	3.722438907229253E-5
44-45	0.1800998664182118	5.790460522356616E-5	2.068021615127363E-6	4.136043230254726E-6	3.929241068741989E-5
46-47	0.18835127266256996	5.790460522356616E-5	4.136043230254726E-6	4.136043230254726E-6	4.136043230254726E-5
48-49	0.19656545451785584	6.20406484538209E-5	4.136043230254726E-6	4.136043230254726E-6	4.136043230254726E-5
50-51	0.20487062932420735	6.20406484538209E-5	4.136043230254726E-6	4.136043230254726E-6	4.136043230254726E-5
52-53	0.21320889247640087	6.20406484538209E-5	4.136043230254726E-6	4.136043230254726E-6	4.3428453917674625E-5
54-55	0.22216963013474775	6.20406484538209E-5	4.136043230254726E-6	4.136043230254726E-6	4.963251876305671E-5
56-57	0.2320072089579086	6.824471329920298E-5	4.136043230254726E-6	4.136043230254726E-6	4.963251876305671E-5
58-59	0.2435922660458521	7.031273491433035E-5	4.136043230254726E-6	8.272086460509452E-6	5.3768561993311436E-5
60-61	0.2564553604919443	7.238075652945771E-5	4.136043230254726E-6	8.272086460509452E-6	5.3768561993311436E-5
62-63	0.27067507711756006	7.85848213748398E-5	4.136043230254726E-6	8.272086460509452E-6	5.3768561993311436E-5
64-65	0.2872606104708815	7.85848213748398E-5	4.136043230254726E-6	8.272086460509452E-6	6.410867006894825E-5
66-67	0.30661315674524336	7.85848213748398E-5	4.136043230254726E-6	1.2408129690764178E-5	6.824471329920298E-5
68-69	0.32956612865154195	8.272086460509452E-5	4.136043230254726E-6	1.2408129690764178E-5	7.031273491433035E-5
70-71	0.35672752454462475	8.272086460509452E-5	4.136043230254726E-6	1.6544172921018904E-5	7.238075652945771E-5
72-73	0.3882627861537019	8.272086460509452E-5	4.136043230254726E-6	1.6544172921018904E-5	7.444877814458507E-5
74-75	0.4256505489335895	8.272086460509452E-5	4.136043230254726E-6	1.8612194536146266E-5	7.444877814458507E-5
76-77	0.47006958520491016	8.478888622022188E-5	1.2408129690764178E-5	2.068021615127363E-5	7.651679975971243E-5
78-79	0.5206988903864582	8.685690783534925E-5	1.2408129690764178E-5	2.4816259381528355E-5	7.85848213748398E-5
80-81	0.5771910368468924	8.685690783534925E-5	1.2408129690764178E-5	2.895230261178308E-5	7.85848213748398E-5
82-83	0.6419015012058428	8.685690783534925E-5	1.6544172921018904E-5	2.895230261178308E-5	7.85848213748398E-5
84-85	0.7156926484768174	8.685690783534925E-5	2.068021615127363E-5	2.895230261178308E-5	7.85848213748398E-5
86-87	0.7987919610374801	8.892492945047662E-5	2.2748237766400992E-5	2.895230261178308E-5	8.685690783534925E-5
88	0.8680272566903291	9.099295106560397E-5	2.4816259381528355E-5	2.895230261178308E-5	9.099295106560397E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	17000	0.0	54.58218	1
TCTCGAG	21130	0.0	43.73846	2
CTCGAGG	26990	0.0	34.22332	3
TCGAGGG	31545	0.0	29.356266	4
GATCCGG	27405	0.0	29.087915	1
ATCCGGC	31910	0.0	24.984955	2
TATCTCG	7665	0.0	24.163708	2
ATGGATC	17035	0.0	20.512537	1
CGAGGGC	47660	0.0	19.726093	5
TCCGGCG	42215	0.0	19.074493	3
GGATCCG	17810	0.0	18.845026	3
AGGGCGC	49125	0.0	18.770634	7
GCGCCGT	45385	0.0	17.593447	9
TGGATCC	20285	0.0	17.334291	2
CGGCGCG	46650	0.0	17.079817	5
GGGCGCG	54980	0.0	16.711401	8
CCGGCGC	50810	0.0	15.764693	4
GAGGGCG	61775	0.0	15.163032	6
ATATCTC	12880	0.0	14.532485	1
CGTCGAC	44125	0.0	14.085751	12-13
>>END_MODULE