FastQCFastQC Report
Wed 25 May 2016
SRR491013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33877559
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA364710.1076553360884118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA209400.042.6735081
TCTCGAG255100.034.8832552
CTCGAGG321850.027.5306643
GATCCGG354650.027.03951
TCGAGGG389350.022.8181514
TATCTCG134650.021.680142
ATCCGGC446600.021.5675132
TCCGGCG532400.018.1174953
GGATCCG236000.017.329422
ATATCTC177150.017.3096311
GCGCCGT565600.017.0005939
CGAGGGC554800.016.0473145
CGGCGCG620950.015.4278865
CGTCGAC544800.015.04027812-13
TGGATCC289050.014.6762451
GCCGTCG568000.014.53343510-11
TCGACTT569750.014.42706914-15
CCGGCGC670050.014.4236364
AGGGCGC613700.014.3743917
ATGGATC258650.014.1574113
Produced by FastQC (version 0.11.5)