##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491013_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33877559
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.618998257814265	33.0	31.0	34.0	30.0	34.0
2	31.906953154446576	34.0	31.0	34.0	30.0	34.0
3	31.996056475025252	34.0	31.0	34.0	30.0	34.0
4	35.34810887643942	37.0	35.0	37.0	33.0	37.0
5	35.13973368033984	37.0	35.0	37.0	32.0	37.0
6	35.08553845334606	37.0	35.0	37.0	32.0	37.0
7	35.00956031100115	37.0	35.0	37.0	32.0	37.0
8	34.92173931421682	37.0	35.0	37.0	32.0	37.0
9	36.47446600270108	39.0	37.0	39.0	32.0	39.0
10-11	36.32591464160686	39.0	37.0	39.0	32.0	39.0
12-13	36.10657193453637	39.0	37.0	39.0	31.5	39.0
14-15	37.43540886461153	40.0	38.0	41.0	32.0	41.0
16-17	37.35243570825159	40.0	38.0	41.0	32.0	41.0
18-19	37.23023538974576	40.0	38.0	41.0	31.0	41.0
20-21	37.08079794060723	40.0	38.0	41.0	31.0	41.0
22-23	36.88106632771269	40.0	38.0	41.0	30.5	41.0
24-25	36.67883962655043	40.0	37.0	41.0	30.0	41.0
26-27	36.349984055817	40.0	37.0	41.0	29.5	41.0
28-29	36.024198024420826	40.0	36.0	41.0	28.5	41.0
30-31	35.72223122687204	39.5	35.5	41.0	27.0	41.0
32-33	35.49250748851179	39.0	35.0	41.0	25.5	41.0
34-35	35.195632512956436	39.0	35.0	41.0	25.0	41.0
36-37	34.892694984311	39.0	35.0	40.0	24.0	41.0
38-39	34.519365872848155	39.0	35.0	40.0	22.0	41.0
40-41	34.03911648415991	38.0	34.0	40.0	16.5	41.0
42-43	33.575003204923945	38.0	33.0	40.0	11.0	41.0
44-45	33.08778049799869	38.0	33.0	40.0	4.5	41.0
46-47	32.563048742679484	38.0	32.5	40.0	2.0	41.0
48-49	32.01777290093421	38.0	31.5	40.0	2.0	41.0
50-51	31.504244018879874	37.0	31.0	40.0	2.0	41.0
52-53	30.94397332464243	37.0	30.0	40.0	2.0	41.0
54-55	30.334462719111492	36.0	28.5	39.0	2.0	40.0
56-57	29.798796454018426	36.0	28.0	39.0	2.0	40.0
58-59	29.189879220636882	35.0	26.5	39.0	2.0	40.0
60-61	28.586432968798018	34.5	26.0	38.0	2.0	40.0
62-63	27.937662185165113	34.0	23.5	38.0	2.0	40.0
64-65	27.23762799734184	34.0	21.5	37.5	2.0	40.0
66-67	26.547411237627834	33.0	19.0	37.0	2.0	39.0
68-69	25.835451574300265	32.5	15.0	36.0	2.0	39.0
70-71	25.127517953699083	32.0	7.5	36.0	2.0	38.5
72-73	24.328530990086975	31.0	4.5	35.0	2.0	38.0
74-75	23.550286651408385	30.5	2.0	34.5	2.0	37.0
76-77	22.744248943673895	29.5	2.0	34.0	2.0	36.0
78-79	22.879011840847213	30.0	2.0	34.0	2.0	36.0
80-81	22.217417937343125	29.5	2.0	34.0	2.0	36.0
82-83	21.43376724692591	29.0	2.0	34.0	2.0	35.0
84-85	20.774988186132298	29.0	2.0	34.0	2.0	35.0
86-87	19.888228310664296	26.5	2.0	33.0	2.0	35.0
88-89	18.94009187615908	25.5	2.0	32.5	2.0	34.0
90-91	18.1882678442092	24.5	2.0	32.0	2.0	34.0
92-93	17.321620707678495	21.5	2.0	32.0	2.0	34.0
94-95	16.332798387274597	15.0	2.0	31.0	2.0	34.0
96-97	15.462421569393474	2.0	2.0	31.0	2.0	34.0
98-99	14.641513191667677	2.0	2.0	31.0	2.0	34.0
100	13.92004843088016	2.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.08256737779074186
1101	2	0.06498930496577415
1101	3	0.09354723598373482
1101	4	0.13466282350053405
1101	5	0.13226703810276064
1101	6	0.10208635255494158
1101	7	0.11326890505633713
1101	8	0.15964079460415093
1101	9	0.09926667516898391
1101	10-11	0.1281605478606025
1101	12-13	0.09537991485116493
1101	14-15	0.10506785041792455
1101	16-17	0.12862122179388535
1101	18-19	0.11628383992279367
1101	20-21	0.09953980992010258
1101	22-23	0.1071669315863204
1101	24-25	0.04298560699490395
1101	26-27	0.034580838251443424
1101	28-29	0.07933111345430888
1101	30-31	0.031226467571990213
1101	32-33	-0.0027783355176751456
1101	34-35	-0.009453152097187001
1101	36-37	-0.06015687800324798
1101	38-39	-0.07443800672385237
1101	40-41	-0.048970218687060196
1101	42-43	-0.1947658265932546
1101	44-45	-0.22790426198633185
1101	46-47	-0.11064386165755735
1101	48-49	-0.26116252575512533
1101	50-51	-0.2848738350235749
1101	52-53	-0.3150031936410258
1101	54-55	-0.34113347297959606
1101	56-57	-0.3417298859303024
1101	58-59	-0.3567723774159788
1101	60-61	-0.47080437463419145
1101	62-63	-0.370307540473803
1101	64-65	-0.38872927408760205
1101	66-67	-0.482297028686844
1101	68-69	-0.5997831714860311
1101	70-71	-0.7580006188233952
1101	72-73	-0.6229022028764071
1101	74-75	-0.5719867932352933
1101	76-77	-0.686749272287873
1101	78-79	-0.6516242878508614
1101	80-81	-0.728777974543263
1101	82-83	-0.7324844197118203
1101	84-85	-0.7548836459216623
1101	86-87	-0.411684655652266
1101	88-89	-0.5548319233598313
1101	90-91	-0.5547580404397863
1101	92-93	-0.47583350940385927
1101	94-95	-0.35202066006450394
1101	96-97	-0.49175124773706536
1101	98-99	-0.3637039648598783
1101	100	-0.353148581681328
1102	1	0.1146821431009144
1102	2	0.11295484367808584
1102	3	0.08093391568055353
1102	4	0.08261145100829026
1102	5	0.0787395365938579
1102	6	0.10369879319461717
1102	7	0.10934352484746057
1102	8	0.13721134736696428
1102	9	0.12575295420258215
1102	10-11	0.12168101669415421
1102	12-13	0.1065294725510455
1102	14-15	0.0951200138832462
1102	16-17	0.10346045359160883
1102	18-19	0.07415952033333895
1102	20-21	0.03651483837583669
1102	22-23	0.08385102457374671
1102	24-25	-0.009618415021932947
1102	26-27	0.021542542241263618
1102	28-29	0.055406442652504495
1102	30-31	-0.00894448635218481
1102	32-33	0.016017401660676
1102	34-35	0.044252710635326764
1102	36-37	0.059289961569980676
1102	38-39	0.026271004058202152
1102	40-41	0.015821695016271065
1102	42-43	-0.05580105369543986
1102	44-45	-0.042221255845966255
1102	46-47	0.03867814991691887
1102	48-49	-0.09095863103149782
1102	50-51	-0.15199031619286885
1102	52-53	-0.2008308949101547
1102	54-55	-0.1436055187997063
1102	56-57	-0.19556534624590327
1102	58-59	-0.34501567541321165
1102	60-61	-0.4011649242259381
1102	62-63	-0.23869947267262503
1102	64-65	-0.1919491688297228
1102	66-67	-0.2970103646349287
1102	68-69	-0.3544365695141565
1102	70-71	-0.40603883279835884
1102	72-73	-0.400364854852473
1102	74-75	-0.4268717404308795
1102	76-77	-0.5459893578965911
1102	78-79	-0.5645343124035449
1102	80-81	-0.5349303104624177
1102	82-83	-0.5117889394231661
1102	84-85	-0.4575826195256809
1102	86-87	-0.21356996239379455
1102	88-89	-0.3475372290182399
1102	90-91	-0.31714612228819306
1102	92-93	-0.3127416188762524
1102	94-95	-0.1782379679960755
1102	96-97	-0.32705061528344537
1102	98-99	-0.21755745252182557
1102	100	-0.19505838425116906
1103	1	0.027997807917014228
1103	2	0.01749906229914444
1103	3	0.019054502581191457
1103	4	-0.08035936870752636
1103	5	0.004450912459383005
1103	6	0.028410013346402252
1103	7	0.02187936449305994
1103	8	0.02775781721290116
1103	9	0.058998166633365656
1103	10-11	0.029507278008772175
1103	12-13	-0.05065439702755725
1103	14-15	-0.0591007975042146
1103	16-17	0.004367921643456896
1103	18-19	0.030458382515732296
1103	20-21	-0.04160653205597242
1103	22-23	-0.06565239719878946
1103	24-25	-0.0455556627888285
1103	26-27	-0.02972065554651948
1103	28-29	-0.0014042885149834206
1103	30-31	-0.06666815581328223
1103	32-33	-0.044561628401879716
1103	34-35	0.004907591986558657
1103	36-37	0.004171193828703679
1103	38-39	-0.11415163267724182
1103	40-41	-0.058531282640117865
1103	42-43	-0.12659836603769037
1103	44-45	-0.13139603102373343
1103	46-47	-0.16868567499825815
1103	48-49	-0.2473805944738352
1103	50-51	-0.3463431256096712
1103	52-53	-0.4993056875295139
1103	54-55	-0.38754296323951465
1103	56-57	-0.36319272611759246
1103	58-59	-0.42231685596068047
1103	60-61	-0.3776784798044943
1103	62-63	-0.23345722916400646
1103	64-65	-0.21562578279270284
1103	66-67	-0.2645658219750793
1103	68-69	-0.31791584647774584
1103	70-71	-0.3337281247943942
1103	72-73	-0.32401236201712535
1103	74-75	-0.30355755335896006
1103	76-77	-0.4585973780926196
1103	78-79	-0.36903947269419035
1103	80-81	-0.45887705998021033
1103	82-83	-0.38734597087838196
1103	84-85	-0.2473521575843307
1103	86-87	0.037495570079119744
1103	88-89	-0.07452904431545804
1103	90-91	-0.11196903919227807
1103	92-93	-0.20089180342168333
1103	94-95	-0.34585513332121565
1103	96-97	-0.5515135331547629
1103	98-99	-0.5817882940084118
1103	100	-0.534620581435906
1104	1	0.0229833253041285
1104	2	0.022650439033338188
1104	3	0.03292494572813354
1104	4	0.05112046414846816
1104	5	0.013110913076658903
1104	6	0.04082200504355882
1104	7	0.0025445006334692266
1104	8	0.0027432244310503506
1104	9	-0.01767006467707688
1104	10-11	-0.0012935255440993387
1104	12-13	-0.012035526825819431
1104	14-15	0.04615664320755286
1104	16-17	0.055091978241229356
1104	18-19	0.052972291430869234
1104	20-21	0.04663694198997348
1104	22-23	0.06231758731708226
1104	24-25	-0.02465800455563283
1104	26-27	0.04991054421701335
1104	28-29	0.06649657145023014
1104	30-31	0.0028925966233614986
1104	32-33	0.09098151366262641
1104	34-35	0.11944584429257077
1104	36-37	0.12287946778296543
1104	38-39	0.1487633951858669
1104	40-41	0.22005246641977294
1104	42-43	0.20907936853736686
1104	44-45	0.15254222011497376
1104	46-47	0.18632468303387384
1104	48-49	0.1658199035135155
1104	50-51	0.17934818088579618
1104	52-53	0.09866761311548089
1104	54-55	0.2098716263156426
1104	56-57	0.12809276533331726
1104	58-59	0.06637786381274324
1104	60-61	0.14575189850155112
1104	62-63	0.18459094848225632
1104	64-65	0.24932289426458
1104	66-67	0.1629131253811913
1104	68-69	0.012415112058739908
1104	70-71	-0.024587704261971766
1104	72-73	0.12911977764834148
1104	74-75	0.1830876895558191
1104	76-77	-0.0034203254272462402
1104	78-79	0.06039164198691793
1104	80-81	-0.041277846746176294
1104	82-83	0.02958299995743019
1104	84-85	0.1869540298511545
1104	86-87	0.42116376895826946
1104	88-89	0.23593802102260497
1104	90-91	0.17873802346252177
1104	92-93	0.15906924657948807
1104	94-95	0.35961527681995875
1104	96-97	-0.07911474595316825
1104	98-99	0.046947866643419545
1104	100	0.11059197020127165
1105	1	8.991505126232369E-6
1105	2	0.01320066137950704
1105	3	-0.007274608314837394
1105	4	0.013435609457097542
1105	5	-0.0012544921560149191
1105	6	4.046838013209708E-4
1105	7	-0.051957586649400866
1105	8	-0.026826454920524156
1105	9	-0.05375588581900104
1105	10-11	-9.098341489846007E-4
1105	12-13	0.01539960746191582
1105	14-15	0.003089989295972373
1105	16-17	-0.015713915968738945
1105	18-19	-0.003110663620979892
1105	20-21	0.008202608995546257
1105	22-23	0.0052089508604495904
1105	24-25	-0.0022168495248209297
1105	26-27	0.010020156431011173
1105	28-29	0.06619786938708927
1105	30-31	0.0600657490766352
1105	32-33	0.019383192302250052
1105	34-35	0.017560541470963642
1105	36-37	0.06415915587086118
1105	38-39	0.05485531529136267
1105	40-41	0.1788386977281604
1105	42-43	0.18386022577625738
1105	44-45	0.10923951704863555
1105	46-47	0.15589926623634653
1105	48-49	0.031180007076372362
1105	50-51	0.1046449098561979
1105	52-53	0.02207354977336351
1105	54-55	0.03652170082535733
1105	56-57	0.07971814806131405
1105	58-59	0.1247645761320264
1105	60-61	0.12355598894380648
1105	62-63	0.2129992384588526
1105	64-65	0.27240153434051706
1105	66-67	0.25204658630321575
1105	68-69	0.11762268580825008
1105	70-71	0.13548708173532376
1105	72-73	0.21786286382852893
1105	74-75	0.25567365994163893
1105	76-77	0.10605092887459833
1105	78-79	0.06747158558466282
1105	80-81	-0.081137962965947
1105	82-83	0.05595160773911445
1105	84-85	0.24282448805667656
1105	86-87	0.4385587440919245
1105	88-89	0.2347984134594121
1105	90-91	0.19488234620273204
1105	92-93	0.28484832556146955
1105	94-95	0.39658427616260283
1105	96-97	0.28150115786765717
1105	98-99	0.3295277363076252
1105	100	0.2970079524367275
1106	1	-0.13317480095818013
1106	2	-0.15004697506560305
1106	3	-0.12469799791912806
1106	4	-0.11995030625133296
1106	5	-0.1670634536638218
1106	6	-0.16410333373847408
1106	7	-0.21064862278937113
1106	8	-0.17845577628266085
1106	9	-0.288631679743105
1106	10-11	-0.2747360778005401
1106	12-13	-0.30115594621151587
1106	14-15	-0.28111613575039485
1106	16-17	-0.2900204493542802
1106	18-19	-0.29000179178235186
1106	20-21	-0.2510791993656767
1106	22-23	-0.2959490055011287
1106	24-25	-0.28090025198798685
1106	26-27	-0.2591746231054515
1106	28-29	-0.25280172309759763
1106	30-31	-0.2536724972141826
1106	32-33	-0.28397711204651443
1106	34-35	-0.3068289269896738
1106	36-37	-0.38726415467476727
1106	38-39	-0.3666968537106925
1106	40-41	-0.21619486423993806
1106	42-43	-0.25042311848211085
1106	44-45	-0.3048387490653752
1106	46-47	-0.2602774695459118
1106	48-49	-0.378742542345325
1106	50-51	-0.2554047372611983
1106	52-53	-0.3015057639914005
1106	54-55	-0.3075629009728438
1106	56-57	-0.21669560136016486
1106	58-59	-0.18133210787064868
1106	60-61	-0.12419675075991421
1106	62-63	-0.12254051660466203
1106	64-65	-0.03764561199641392
1106	66-67	-0.08854305289332842
1106	68-69	-0.10708201487817703
1106	70-71	-0.15259183373047236
1106	72-73	-0.10077862985691866
1106	74-75	-0.19339623339818957
1106	76-77	-0.42956926287100927
1106	78-79	-0.2533593427460801
1106	80-81	-0.30078740552433914
1106	82-83	-0.13006250122348106
1106	84-85	0.023619705666845903
1106	86-87	0.09496759237575958
1106	88-89	-0.09007895066636706
1106	90-91	0.1329327347323499
1106	92-93	0.22064095828392283
1106	94-95	0.42641729763293057
1106	96-97	0.2208879407892912
1106	98-99	0.2968578648060163
1106	100	0.1588382038034606
1107	1	-0.2142670586993809
1107	2	-0.20021723398694746
1107	3	-0.1958417938765571
1107	4	-0.20004968457263317
1107	5	-0.2211808743659205
1107	6	-0.20115087360963457
1107	7	-0.29689658998748314
1107	8	-0.23045635006789666
1107	9	-0.2733173366160102
1107	10-11	-0.28368957392926575
1107	12-13	-0.27132554097199346
1107	14-15	-0.3109155953343148
1107	16-17	-0.2784602644215468
1107	18-19	-0.2954678648225624
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2207	70-71	0.7625487288310531
2207	72-73	0.812385448928822
2207	74-75	0.7518534777479147
2207	76-77	0.7045148198295159
2207	78-79	0.7815759198904004
2207	80-81	0.8226235653569738
2207	82-83	0.9236193317393848
2207	84-85	0.8705482565891103
2207	86-87	0.8840573286929292
2207	88-89	0.9730753706924098
2207	90-91	0.98686145777517
2207	92-93	0.6741305373128164
2207	94-95	0.5062463178315184
2207	96-97	0.6562469394712966
2207	98-99	0.6659269806365007
2207	100	0.5210145629863039
2208	1	-0.7475529861493548
2208	2	-0.6964580215595504
2208	3	-0.4509702682116341
2208	4	-0.3021242483656934
2208	5	-0.3483664504724544
2208	6	-0.3448533972237655
2208	7	-0.33198708036145064
2208	8	-0.32882485223076685
2208	9	-0.3887837691055296
2208	10-11	-0.37270102625429047
2208	12-13	-0.3401970920795847
2208	14-15	-0.4934383909389197
2208	16-17	-0.4924656804394161
2208	18-19	-0.45204457215655935
2208	20-21	-0.43702988724486147
2208	22-23	-0.43328283601159967
2208	24-25	-0.3929796408018973
2208	26-27	-0.3913673965943758
2208	28-29	-0.41034182749790205
2208	30-31	-0.47111292075437206
2208	32-33	-0.29093330712734655
2208	34-35	-0.2816589104698366
2208	36-37	-0.28259263471466056
2208	38-39	-0.2665982279525423
2208	40-41	-0.1951906087640225
2208	42-43	-0.10391356355295756
2208	44-45	-0.2167486383577426
2208	46-47	-0.3446521829530411
2208	48-49	-0.23659997109443154
2208	50-51	-0.05759282984206848
2208	52-53	0.03823495860319781
2208	54-55	0.1612266427331015
2208	56-57	0.11551889236405799
2208	58-59	0.18479904765566957
2208	60-61	0.08867226694724195
2208	62-63	0.03797167735030982
2208	64-65	-0.17846733870921128
2208	66-67	0.051388654663483635
2208	68-69	0.1019866587905156
2208	70-71	-0.0875318475924125
2208	72-73	-0.013996438792055699
2208	74-75	-0.04802392307308523
2208	76-77	-0.13339322134557818
2208	78-79	-0.17874614767037755
2208	80-81	-0.2870044293046199
2208	82-83	-0.291535286974824
2208	84-85	-0.42170966260303544
2208	86-87	-0.4918441892509975
2208	88-89	-0.44219040508825813
2208	90-91	-0.7028260089517602
2208	92-93	-1.544331958233002
2208	94-95	-3.7017210168244734
2208	96-97	-2.85417686987598
2208	98-99	-2.3731493631496345
2208	100	-2.453782133792709
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	479587.0
3	203407.0
4	194127.0
5	157903.0
6	147979.0
7	152149.0
8	159730.0
9	181075.0
10	210536.0
11	239877.0
12	270272.0
13	315958.0
14	390277.0
15	496097.0
16	587359.0
17	590281.0
18	551301.0
19	523672.0
20	534841.0
21	557296.0
22	586746.0
23	619476.0
24	655757.0
25	699719.0
26	753987.0
27	827581.0
28	925762.0
29	1056604.0
30	1208529.0
31	1392038.0
32	1628787.0
33	1966646.0
34	2505849.0
35	3362769.0
36	4180688.0
37	3489916.0
38	1040606.0
39	32370.0
40	5.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.568214881739006	27.962319659695282	28.171155735057386	22.298309723508325
2	23.44754181273018	27.81134705198515	27.136255173335428	21.604855961949248
3	23.341962653469007	26.43167487682984	26.93830281103188	23.28805965866927
4	24.17948451902557	25.989207101416138	26.705606666252425	23.125701713305865
5	24.450041009317225	26.27607677246503	26.46521178483277	22.808670433384975
6	24.41226196222758	26.203230814573548	25.80950436565927	23.575002857539605
7	24.402257909977674	26.4856643651527	26.036690107278265	23.07538761759136
8	23.872260855352774	26.364646079208313	26.309609107026382	23.453483958412534
9	23.839359008758905	26.072057201336698	26.434226462200755	23.654357327703643
10-11	23.698987210860547	26.300820920463114	26.400006694740892	23.600185173935447
12-13	23.43757301148628	26.337411030222974	26.560424176902725	23.66459178138802
14-15	23.12641532973278	26.575053806753164	27.047881106760418	23.250649756753642
16-17	22.95855838400211	26.50846637420596	27.22973102015502	23.303244221636916
18-19	22.575412123474035	26.44581957795178	27.90354207449079	23.075226224083394
20-21	22.294243666015927	26.224835033864608	28.657241306674898	22.82367999344457
22-23	22.30704311913146	26.181475950515708	29.01059757750259	22.50088335285024
24-25	22.242880100587335	26.031835859201998	29.399040185503505	22.326243854707165
26-27	22.26807161733275	26.08600678015894	29.435562572417474	22.21035903009084
28-29	22.32691729862192	26.147788309848828	29.38924109549033	22.136053296038913
30-31	22.28393840802881	26.09085221779988	29.359368870491664	22.265840503679645
32-33	22.315700156832968	26.166881807642934	29.241953032056067	22.27546500346803
34-35	22.391081533094443	26.21927464728686	29.253979334645468	22.13566448497323
36-37	22.42057287292681	26.19017376500366	29.13701790086945	22.252235461200083
38-39	22.446683050548792	26.268093746694344	28.998969539035464	22.286253663721403
40-41	22.55214914837754	26.414098363883433	28.73032447734179	22.303428010397234
42-43	22.665678150054685	26.508419880932276	28.312473029285957	22.51342893972708
44-45	22.72060402135593	26.736395650069987	27.907603881997538	22.635396446576543
46-47	22.97463557543668	26.89805088872256	27.446212191509183	22.681101344331577
48-49	23.032247166957596	26.918933027776106	27.156821093273166	22.891998711993143
50-51	23.084722326319067	26.985985463885818	26.974416815707595	22.95487539408752
52-53	23.204644942675156	27.028641552014715	26.83123234436906	22.93548116094107
54-55	23.249885435641886	26.98458240990749	26.750398678357755	23.01513347609287
56-57	23.23608030495742	27.04416785645988	26.72571205354675	22.994039785035948
58-59	23.332179893259195	27.136251824527452	26.621244890234642	22.91032339197871
60-61	23.290295761756457	27.098527157622982	26.590667627096792	23.02050945352377
62-63	23.29937160600917	27.169563889575848	26.535520073498496	22.995544430916482
64-65	23.362942167847137	27.25397361587455	26.443992834038905	22.93909138223941
66-67	23.401641781946953	27.271757728609035	26.306734482158582	23.01986600728543
68-69	23.36303222360121	27.402527422707735	26.187580602985566	23.046859750705487
70-71	23.447212937518298	27.464566232780168	26.12563246528729	22.96258836441424
72-73	23.43045342149225	27.512624642259674	25.94485251331649	23.112069422931587
74-75	23.519638455055237	27.574179676805606	25.88358299182858	23.022598876310578
76-77	23.054556255192903	27.98008675978046	25.70278052294085	23.26257646208579
78-79	23.287514577962497	27.929257390491024	25.624009117356444	23.15921891419003
80-81	23.219402706257718	28.010148019975883	25.434496032952946	23.33595324081345
82-83	23.233702201771898	28.091428697939918	25.36435911722489	23.310509983063294
84-85	23.229063212494854	28.146786903417116	25.250852575160504	23.373297308927533
86-87	23.10716695364952	28.300109728767474	25.043017909790517	23.549705407792487
88-89	23.285704987434777	28.275166199366623	24.932806647295745	23.506322165902855
90-91	23.0743026629134	28.377035962260557	24.908899344566464	23.639762030259583
92-93	23.049936188523617	28.512115915351167	24.785115388299513	23.652832507825707
94-95	23.08572854283168	28.478914394730964	24.63863301638349	23.796724046053864
96-97	23.01618431197247	28.617368706819402	24.379396119270208	23.987050861937924
98-99	22.97840461587165	28.67538192685269	24.30824733468758	24.037966122588085
100	23.706539731433935	28.73751018543544	23.63975952395037	23.916190559180258
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3473.0
1	2632.5
2	1608.5
3	1461.5
4	1551.0
5	1779.5
6	2129.5
7	2429.5
8	2813.0
9	3362.0
10	4111.5
11	5131.0
12	6561.0
13	8775.5
14	13480.0
15	18577.5
16	25277.0
17	29199.5
18	30745.0
19	37280.0
20	53578.0
21	66432.5
22	74406.0
23	91376.0
24	112244.5
25	136327.0
26	165062.5
27	201495.0
28	243612.0
29	284506.5
30	325007.5
31	373716.0
32	432017.5
33	494168.0
34	559736.0
35	637689.0
36	724911.5
37	823954.0
38	923734.0
39	1004009.0
40	1072933.5
41	1154652.0
42	1271971.0
43	1379869.5
44	1442387.0
45	1486208.5
46	1484553.5
47	1427864.0
48	1368481.0
49	1335163.5
50	1298116.5
51	1222408.0
52	1117039.0
53	1007448.5
54	915108.5
55	836049.0
56	752512.0
57	671086.5
58	608312.0
59	553336.0
60	494021.0
61	435307.5
62	384484.0
63	341385.0
64	302439.5
65	263940.5
66	227167.0
67	193652.5
68	163044.0
69	136147.5
70	113387.5
71	94272.0
72	78390.5
73	65297.5
74	53845.5
75	43502.0
76	34558.0
77	27466.5
78	22199.5
79	17749.5
80	13896.5
81	10535.0
82	7833.0
83	5762.0
84	4098.5
85	2847.0
86	1952.5
87	1393.0
88	998.5
89	661.5
90	431.0
91	292.0
92	191.5
93	127.5
94	77.5
95	44.5
96	24.5
97	8.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05874980543905185
2	0.006405420178000428
3	1.0331322867742626E-4
4	0.0019304814730010508
5	0.01767541752344081
6	0.006679938185629018
7	0.004286023086846369
8	9.79999769168729E-4
9	8.560238947558176E-5
10-11	6.523492439346058E-4
12-13	5.43132402189898E-4
14-15	2.1695778022259513E-4
16-17	5.54939628324461E-4
18-19	0.0010729816749784126
20-21	8.943973796931473E-4
22-23	0.002801264400425072
24-25	0.0039126195603408145
26-27	0.003149577571394681
28-29	0.002420481357585415
30-31	0.002091354929084472
32-33	0.005046113269258862
34-35	0.0035377401305684395
36-37	0.0019393368926019726
38-39	0.0028706318539656293
40-41	0.0019585236350706378
42-43	0.001582168302031442
44-45	0.002585782523469297
46-47	0.0022448488688337907
48-49	0.005794396225536793
50-51	0.0044808423180666595
52-53	0.00287210775723245
54-55	9.224395417627344E-4
56-57	5.121384335866701E-4
58-59	0.0018788248586623377
60-61	0.0011113551599157424
62-63	0.001195481646124504
64-65	0.002994607728378541
66-67	0.0010154214475724182
68-69	0.0014153912328807397
70-71	8.988250894936084E-4
72-73	0.0025282222960633025
74-75	0.004083824339291978
76-77	0.0023673488399798817
78-79	0.004461655575597994
80-81	0.0012058129689922464
82-83	0.0042328905692408355
84-85	0.0010050901247046753
86-87	0.0011512045481198924
88-89	9.56385316899603E-4
90-91	1.726806822179839E-4
92-93	0.001251565970263678
94-95	0.00515680601427039
96-97	0.003006414954513104
98-99	0.0018315959541240856
100	0.0022286139328987664
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.3877559E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.367209086841854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8926386837015	31.808338616676647
2	12.12351796037673	10.030322046699736
3	4.339735973508133	5.385682961934004
4	1.9925761249248533	3.2970925272486205
5	1.1214255716405708	2.319512304869332
6	0.6818355157934769	1.6923379406798056
7	0.45424933031907033	1.315371891740681
8	0.3252208970751251	1.0762784678973578
9	0.24312976946727055	0.9051840007909415
>10	1.4904452693580883	12.149025786063216
>50	0.13681194467091315	3.904516310374613
>100	0.17132769758084906	17.554575940970512
>500	0.02465233925192162	6.586348484613411
>1k	0.0023041475485504593	1.2122590551287606
>5k	4.29249277335945E-5	0.11331826324394804
>10k+	8.584985506051973E-5	0.6498354010686231
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36471	0.1076553360884118	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.95180653364075E-6	0.0	0.0	5.9036130672815E-6	0.0
3	2.95180653364075E-6	2.95180653364075E-6	0.0	5.9036130672815E-6	0.0
4	2.95180653364075E-6	2.95180653364075E-6	0.0	5.9036130672815E-6	5.9036130672815E-6
5	2.95180653364075E-6	5.9036130672815E-6	0.0	5.9036130672815E-6	5.9036130672815E-6
6	2.95180653364075E-6	8.855419600922251E-6	0.0	5.9036130672815E-6	5.9036130672815E-6
7	2.95180653364075E-6	8.855419600922251E-6	0.0	5.9036130672815E-6	5.9036130672815E-6
8	5.9036130672815E-6	1.1807226134563E-5	0.0	8.855419600922251E-6	5.9036130672815E-6
9	1.7710839201844502E-5	2.066264573548525E-5	0.0	8.855419600922251E-6	8.855419600922251E-6
10-11	4.7228904538252E-5	2.6566258802766753E-5	0.0	1.4759032668203751E-5	1.3283129401383375E-5
12-13	2.199095867562359E-4	3.394577513686863E-5	0.0	1.4759032668203751E-5	1.4759032668203751E-5
14-15	6.464456308673244E-4	3.8373484937329753E-5	0.0	1.7710839201844502E-5	1.4759032668203751E-5
16-17	0.001589547818365544	4.7228904538252E-5	0.0	1.7710839201844502E-5	1.6234935935024128E-5
18-19	0.0028735836604992706	4.7228904538252E-5	0.0	1.7710839201844502E-5	2.066264573548525E-5
20-21	0.004604818192479571	5.4608420872353884E-5	0.0	2.066264573548525E-5	2.509035553594638E-5
22-23	0.006830480318844696	5.6084324139174256E-5	0.0	2.2138549002305626E-5	2.6566258802766753E-5
24-25	0.009622889299668844	6.198793720645575E-5	0.0	2.6566258802766753E-5	3.2469871870048255E-5
26-27	0.012664725932585639	6.198793720645575E-5	0.0	3.099396860322788E-5	3.2469871870048255E-5
28-29	0.01592352034572503	6.198793720645575E-5	1.475903266820375E-6	3.8373484937329753E-5	3.2469871870048255E-5
30-31	0.018577194419468063	6.346384047327614E-5	2.95180653364075E-6	4.13252914709705E-5	3.5421678403689004E-5
32-33	0.020888458935308767	6.936745354055762E-5	2.95180653364075E-6	4.2801194737790874E-5	3.5421678403689004E-5
34-35	0.02328384993735824	7.231926007419838E-5	2.95180653364075E-6	4.427709800461125E-5	3.5421678403689004E-5
36-37	0.025791409587686054	8.117467967512062E-5	4.4277098004611255E-6	4.427709800461125E-5	3.5421678403689004E-5
38-39	0.028160234330932756	8.855419600922252E-5	5.9036130672815E-6	4.575300127143163E-5	3.5421678403689004E-5
40-41	0.03030176997108912	9.593371234332439E-5	5.9036130672815E-6	4.870480780507237E-5	3.5421678403689004E-5
42-43	0.03285951033248884	1.0331322867742626E-4	5.9036130672815E-6	5.018071107189275E-5	3.6897581670509375E-5
44-45	0.03576261205832451	1.0331322867742626E-4	5.9036130672815E-6	5.018071107189275E-5	3.8373484937329753E-5
46-47	0.03923688834841967	1.0331322867742626E-4	5.9036130672815E-6	5.3132517605533506E-5	3.8373484937329753E-5
48-49	0.04293402603180471	1.1069274501152813E-4	5.9036130672815E-6	5.9036130672815005E-5	3.8373484937329753E-5
50-51	0.04725989850685523	1.1216864827834851E-4	5.9036130672815E-6	5.9036130672815005E-5	3.8373484937329753E-5
52-53	0.05271336107775652	1.1512045481198925E-4	8.855419600922251E-6	6.493974374009651E-5	3.8373484937329753E-5
54-55	0.059105498126355564	1.1512045481198925E-4	8.855419600922251E-6	6.493974374009651E-5	3.8373484937329753E-5
56-57	0.06701781553977959	1.1807226134563001E-4	8.855419600922251E-6	6.493974374009651E-5	3.984938820415013E-5
58-59	0.07646212054416318	1.2545177767973187E-4	8.855419600922251E-6	6.789155027373725E-5	4.2801194737790874E-5
60-61	0.08769226850139941	1.2692768094655225E-4	8.855419600922251E-6	7.084335680737801E-5	4.870480780507237E-5
62-63	0.10162331943691692	1.3283129401383376E-4	8.855419600922251E-6	7.084335680737801E-5	5.018071107189275E-5
64-65	0.1188382551411098	1.357831005474745E-4	8.855419600922251E-6	7.084335680737801E-5	5.165661433871313E-5
66-67	0.1393178888715093	1.4168671361475602E-4	8.855419600922251E-6	7.084335680737801E-5	5.3132517605533506E-5
68-69	0.16447318415119577	1.4463852014839676E-4	1.1807226134563E-5	7.379516334101875E-5	5.3132517605533506E-5
70-71	0.1953623636224794	1.4463852014839676E-4	1.1807226134563E-5	7.379516334101875E-5	5.6084324139174256E-5
72-73	0.23242229465233905	1.4611442341521714E-4	1.3283129401383375E-5	8.117467967512062E-5	5.6084324139174256E-5
74-75	0.2753946941690811	1.549698430161394E-4	1.9186742468664877E-5	8.412648620876139E-5	5.6084324139174256E-5
76-77	0.32651555562193846	1.593975528166005E-4	2.3614452269126E-5	8.560238947558176E-5	5.756022740599463E-5
78-79	0.3862896379281636	1.593975528166005E-4	2.3614452269126E-5	9.4457809076504E-5	5.9036130672815005E-5
80-81	0.45542537465583044	1.6087345608342088E-4	2.3614452269126E-5	9.740961561014476E-5	6.346384047327614E-5
82-83	0.5340600248087533	1.667770691507024E-4	2.509035553594638E-5	1.003614221437855E-4	6.493974374009651E-5
84-85	0.6242657565735477	1.77108392018445E-4	2.9518065336407502E-5	1.0478913194424664E-4	6.493974374009651E-5
86-87	0.7263982626375176	1.8153610181890614E-4	2.9518065336407502E-5	1.1364455154516888E-4	6.641564700691688E-5
88	0.8106044476226874	1.8301200508572652E-4	2.9518065336407502E-5	1.1512045481198925E-4	6.789155027373725E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	20940	0.0	42.673508	1
TCTCGAG	25510	0.0	34.883255	2
CTCGAGG	32185	0.0	27.530664	3
GATCCGG	35465	0.0	27.0395	1
TCGAGGG	38935	0.0	22.818151	4
TATCTCG	13465	0.0	21.68014	2
ATCCGGC	44660	0.0	21.567513	2
TCCGGCG	53240	0.0	18.117495	3
GGATCCG	23600	0.0	17.32942	2
ATATCTC	17715	0.0	17.309631	1
GCGCCGT	56560	0.0	17.000593	9
CGAGGGC	55480	0.0	16.047314	5
CGGCGCG	62095	0.0	15.427886	5
CGTCGAC	54480	0.0	15.040278	12-13
TGGATCC	28905	0.0	14.676245	1
GCCGTCG	56800	0.0	14.533435	10-11
TCGACTT	56975	0.0	14.427069	14-15
CCGGCGC	67005	0.0	14.423636	4
AGGGCGC	61370	0.0	14.374391	7
ATGGATC	25865	0.0	14.157411	3
>>END_MODULE