FastQCFastQC Report
Wed 25 May 2016
SRR491012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36693969
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407090.11094193707963289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA238650.048.379431
TCTCGAG289650.039.7738042
CTCGAGG381100.030.116993
GATCCGG405450.029.276741
TCGAGGG450700.025.372314
ATCCGGC511500.023.3224052
TATCTCG153950.020.4866142
TCCGGCG619550.019.4360333
GCGCCGT667400.017.612369
GGATCCG265950.017.4262642
CGAGGGC674450.017.2546355
ATATCTC192000.017.0971951
CGGCGCG704750.016.8195695
AGGGCGC708750.016.147027
TGGATCC299850.015.9823561
ATGGATC253950.015.6672531
CCGGCGC805500.014.9375684
CGTCGAC640850.014.79255412-13
GCCGTCG666250.014.36966210-11
TCGACTT662750.014.3676114-15
Produced by FastQC (version 0.11.5)