##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491012_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36693969
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71058246656283	33.0	31.0	34.0	30.0	34.0
2	31.985310937609395	34.0	31.0	34.0	30.0	34.0
3	32.05947059038503	34.0	31.0	34.0	30.0	34.0
4	35.430190585270296	37.0	35.0	37.0	33.0	37.0
5	35.20562272781121	37.0	35.0	37.0	32.0	37.0
6	35.159121462167256	37.0	35.0	37.0	32.0	37.0
7	35.09371662683859	37.0	35.0	37.0	32.0	37.0
8	35.01386072463298	37.0	35.0	37.0	32.0	37.0
9	36.56482570746163	39.0	37.0	39.0	32.0	39.0
10-11	36.423240165161744	39.0	37.0	39.0	32.0	39.0
12-13	36.22191120835143	39.0	37.0	39.0	32.0	39.0
14-15	37.50488704288162	40.0	38.0	41.0	32.0	41.0
16-17	37.45168748303025	40.0	38.0	41.0	32.0	41.0
18-19	37.342542721393805	40.0	38.0	41.0	32.0	41.0
20-21	37.1757930029319	40.0	38.0	41.0	31.0	41.0
22-23	37.02616528073047	40.0	38.0	41.0	31.0	41.0
24-25	36.83580286722322	40.0	37.5	41.0	30.0	41.0
26-27	36.508288119499966	40.0	37.0	41.0	30.0	41.0
28-29	36.181532679116835	40.0	36.0	41.0	29.0	41.0
30-31	35.91027904340356	40.0	36.0	41.0	27.5	41.0
32-33	35.68270569204438	40.0	35.0	41.0	27.0	41.0
34-35	35.40026976912746	39.0	35.0	41.0	25.5	41.0
36-37	35.103988927989775	39.0	35.0	40.5	24.5	41.0
38-39	34.71619525541105	39.0	35.0	40.0	23.5	41.0
40-41	34.26357588899691	38.5	34.5	40.0	19.0	41.0
42-43	33.79632201956676	38.0	34.0	40.0	13.5	41.0
44-45	33.34560787087382	38.0	33.0	40.0	8.0	41.0
46-47	32.799883272371	38.0	33.0	40.0	2.0	41.0
48-49	32.30569094338091	38.0	32.0	40.0	2.0	41.0
50-51	31.770891832388042	37.5	31.0	40.0	2.0	41.0
52-53	31.239545822911662	37.0	30.5	40.0	2.0	41.0
54-55	30.650641921564823	36.5	29.5	39.0	2.0	40.5
56-57	30.100421134601163	36.0	28.5	39.0	2.0	40.0
58-59	29.53312451700169	35.0	27.5	39.0	2.0	40.0
60-61	28.911898423961716	35.0	26.0	38.0	2.0	40.0
62-63	28.237747693633253	34.0	25.5	38.0	2.0	40.0
64-65	27.619266874619097	34.0	23.5	38.0	2.0	40.0
66-67	26.89919487859163	33.5	21.0	37.0	2.0	39.0
68-69	26.210195604623745	33.0	19.0	36.0	2.0	39.0
70-71	25.49257950264252	32.0	13.5	36.0	2.0	38.5
72-73	24.721134336817038	31.0	7.5	35.0	2.0	38.0
74-75	24.01866577856432	31.0	3.5	35.0	2.0	37.0
76-77	23.024666519449013	29.5	2.0	34.0	2.0	36.0
78-79	23.13389085819525	30.0	2.0	34.0	2.0	36.0
80-81	22.6039053038934	30.0	2.0	34.0	2.0	35.5
82-83	21.93669214415045	29.5	2.0	34.0	2.0	35.0
84-85	21.086375352309258	29.0	2.0	34.0	2.0	35.0
86-87	20.24712360769695	27.0	2.0	33.0	2.0	35.0
88-89	19.492539863976013	26.0	2.0	33.0	2.0	34.5
90-91	18.70760919866695	25.0	2.0	32.0	2.0	34.0
92-93	17.9288776855946	24.0	2.0	32.0	2.0	34.0
94-95	16.90456614273588	19.0	2.0	31.5	2.0	34.0
96-97	15.869087383269987	8.0	2.0	31.0	2.0	34.0
98-99	14.996080091526757	2.0	2.0	31.0	2.0	34.0
100	14.257340000478008	2.0	2.0	30.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18541640689489114
1101	2	0.22064871327723523
1101	3	0.23116675299816336
1101	4	0.1952306832159607
1101	5	0.23107858374521584
1101	6	0.18888394769787453
1101	7	0.17867011433796876
1101	8	0.19287174353466696
1101	9	0.24834761152622065
1101	10-11	0.25325143613488876
1101	12-13	0.24402998114962315
1101	14-15	0.3068688392992982
1101	16-17	0.2961313244553665
1101	18-19	0.3276469340165491
1101	20-21	0.33955581462905116
1101	22-23	0.27403175270471536
1101	24-25	0.26163523212944995
1101	26-27	0.23259416981426284
1101	28-29	0.20239240658094104
1101	30-31	0.21568846146055876
1101	32-33	0.17209760249241413
1101	34-35	0.12490845144224494
1101	36-37	0.04766107036019207
1101	38-39	0.07017626416163836
1101	40-41	0.01729515467236098
1101	42-43	-0.06257991158085474
1101	44-45	-0.09932759246854772
1101	46-47	-0.2256791576911823
1101	48-49	-0.23557018237083582
1101	50-51	-0.21447895213644586
1101	52-53	-0.2560694009535922
1101	54-55	-0.34924075961523116
1101	56-57	-0.4477899668415368
1101	58-59	-0.4008676779440776
1101	60-61	-0.500206923121354
1101	62-63	-0.4426252219517437
1101	64-65	-0.5147755060984167
1101	66-67	-0.5203133472211441
1101	68-69	-0.7081620817405252
1101	70-71	-0.7942245712232072
1101	72-73	-0.725705912290973
1101	74-75	-0.807581716035525
1101	76-77	-0.816574667260543
1101	78-79	-0.9466860282553853
1101	80-81	-0.8116412256759418
1101	82-83	-1.0004514459851173
1101	84-85	-1.2526286963166555
1101	86-87	-1.1577900455472587
1101	88-89	-1.0786332060300339
1101	90-91	-0.8483976700593274
1101	92-93	-0.9522060605679279
1101	94-95	-0.9629761524367382
1101	96-97	-0.9140908455657559
1101	98-99	-0.7496163708529231
1101	100	-0.44722606184780744
1102	1	0.0903495685185014
1102	2	0.03967808760992142
1102	3	0.08579180767913641
1102	4	0.09209171754471868
1102	5	0.115296352158758
1102	6	0.1381466324031848
1102	7	0.1355680014162246
1102	8	0.12951339260376926
1102	9	0.11554910243398808
1102	10-11	0.12053754928535199
1102	12-13	0.08648367184357397
1102	14-15	0.16369963934003096
1102	16-17	0.1453845045303126
1102	18-19	0.1424320472081888
1102	20-21	0.16898793911511945
1102	22-23	0.12538655474509852
1102	24-25	0.11051701477883569
1102	26-27	0.06122584382440266
1102	28-29	0.014135717638140477
1102	30-31	0.05342227578575631
1102	32-33	0.003885254037385266
1102	34-35	-0.0028385516819184886
1102	36-37	0.030104604190164252
1102	38-39	0.04780852108898159
1102	40-41	0.032283893131044294
1102	42-43	0.04210976354539753
1102	44-45	-0.029141804823943573
1102	46-47	-0.1258032583469344
1102	48-49	-0.15409147243838106
1102	50-51	-0.182887209511712
1102	52-53	-0.31213779597368685
1102	54-55	-0.30661642945314327
1102	56-57	-0.3891279746971996
1102	58-59	-0.35044195922366583
1102	60-61	-0.3074923157835343
1102	62-63	-0.2873927716214446
1102	64-65	-0.3474178987999359
1102	66-67	-0.2812449056317341
1102	68-69	-0.370389438759986
1102	70-71	-0.4359752611569334
1102	72-73	-0.318292565651074
1102	74-75	-0.4068954866861638
1102	76-77	-0.4515972916433597
1102	78-79	-0.5907689460361709
1102	80-81	-0.45856013539392393
1102	82-83	-0.7144757893127753
1102	84-85	-0.9671558749355782
1102	86-87	-0.8540315240769303
1102	88-89	-0.7191320268201622
1102	90-91	-0.5822710517184717
1102	92-93	-0.7169195995349966
1102	94-95	-0.8722453881072489
1102	96-97	-0.8775472844270045
1102	98-99	-0.6982151742048757
1102	100	-0.3664513650083485
1103	1	-0.009107858606824948
1103	2	-0.005746222079366703
1103	3	-0.01995476987280398
1103	4	-0.08791632135186944
1103	5	-0.03341644019234025
1103	6	0.013764605425599541
1103	7	0.01100952162322244
1103	8	-0.01627655453467014
1103	9	-0.018867937559043924
1103	10-11	-0.022669031703081544
1103	12-13	-0.09865805099593672
1103	14-15	-0.059712286424954186
1103	16-17	-0.022783471785750464
1103	18-19	0.02475344233499044
1103	20-21	-0.07517801600377538
1103	22-23	-0.03396904859551597
1103	24-25	0.03731571195826433
1103	26-27	-0.001664022903085538
1103	28-29	-0.029005686146234666
1103	30-31	0.00386973038683891
1103	32-33	-0.04381116240868721
1103	34-35	-0.11893084531639886
1103	36-37	-0.16845710936794234
1103	38-39	-0.3402168164072279
1103	40-41	-0.1950569362325112
1103	42-43	-0.34855046798329425
1103	44-45	-0.3019296693187101
1103	46-47	-0.3769058231236784
1103	48-49	-0.35541866833933256
1103	50-51	-0.649831364146678
1103	52-53	-0.4742279417202795
1103	54-55	-0.4512127943447126
1103	56-57	-0.4629408966828983
1103	58-59	-0.7382872918013099
1103	60-61	-0.5237564185270571
1103	62-63	-0.4092130675363883
1103	64-65	-0.5390302094295798
1103	66-67	-0.5151612081608903
1103	68-69	-0.5106535637173089
1103	70-71	-0.5548045548432121
1103	72-73	-0.33447256972589656
1103	74-75	-0.33077190911573595
1103	76-77	-0.298489378409446
1103	78-79	-0.38596376054959336
1103	80-81	-0.25106901417011684
1103	82-83	-0.45517491610782557
1103	84-85	-0.6344695818162442
1103	86-87	-0.6512913959785003
1103	88-89	-0.33395207445490627
1103	90-91	-0.36198844837736033
1103	92-93	-0.48049956946151795
1103	94-95	-0.5295783361427233
1103	96-97	-0.5803052491653187
1103	98-99	-0.5252594397020527
1103	100	-0.3841960968758613
1104	1	0.005188453484215927
1104	2	0.003892863204740138
1104	3	0.01755394358247031
1104	4	-0.012041171242685778
1104	5	0.008820812840653502
1104	6	0.016896397965282972
1104	7	0.01796842003520993
1104	8	0.04673041076065232
1104	9	0.01047905360466217
1104	10-11	0.011212067697435657
1104	12-13	0.02400222544511621
1104	14-15	0.03076464683388025
1104	16-17	0.045609564920312096
1104	18-19	0.07967404187968441
1104	20-21	0.09807359292026518
1104	22-23	0.052760539922061866
1104	24-25	0.05594825096348188
1104	26-27	0.0882412094863767
1104	28-29	0.06081127489477467
1104	30-31	0.06347664935796615
1104	32-33	0.10853533125592207
1104	34-35	0.09560859818016354
1104	36-37	0.10118984394876662
1104	38-39	0.10753827422721685
1104	40-41	0.14511516078668052
1104	42-43	0.11317049882340058
1104	44-45	0.06579404677177081
1104	46-47	0.06199867125535263
1104	48-49	0.08712209370796131
1104	50-51	0.1372928973423626
1104	52-53	0.13731723738004575
1104	54-55	0.09463076942405735
1104	56-57	0.1584572216632445
1104	58-59	0.09715937934398866
1104	60-61	0.18656961661829996
1104	62-63	0.3275716491236622
1104	64-65	0.2543495665891413
1104	66-67	0.182801801824791
1104	68-69	0.18612424380189196
1104	70-71	0.11784410246050214
1104	72-73	0.19811745922961777
1104	74-75	0.23930302351659805
1104	76-77	0.18596933732734655
1104	78-79	0.08394005131707871
1104	80-81	0.2269156432996482
1104	82-83	0.13286076036330385
1104	84-85	-0.01289143440017071
1104	86-87	0.0833939322177244
1104	88-89	0.323073735082005
1104	90-91	0.3626578318296012
1104	92-93	0.18272535201665718
1104	94-95	0.2021886925752554
1104	96-97	0.3171971530666795
1104	98-99	0.49743750046395974
1104	100	0.6751295372599984
1105	1	-0.1076224486452304
1105	2	-0.10536375522551111
1105	3	-0.10088770697992899
1105	4	-0.09285805158942395
1105	5	-0.09240585334293883
1105	6	-0.12143507020735456
1105	7	-0.11192939320554274
1105	8	-0.10470158133038154
1105	9	-0.17932518053459034
1105	10-11	-0.13762874145430004
1105	12-13	-0.12240320651923042
1105	14-15	-0.2240250416147802
1105	16-17	-0.18018205991989333
1105	18-19	-0.17040134189073086
1105	20-21	-0.21295606413273305
1105	22-23	-0.23680899308058656
1105	24-25	-0.19177052835020447
1105	26-27	-0.1591849227975075
1105	28-29	-0.16240443391983916
1105	30-31	-0.10548589117757956
1105	32-33	-0.14428639278538924
1105	34-35	-0.17260334264816635
1105	36-37	-0.19821893795548107
1105	38-39	-0.14331060631702286
1105	40-41	-0.09659507092592179
1105	42-43	-0.16471910952014923
1105	44-45	-0.12983840233732025
1105	46-47	-0.04056030474298211
1105	48-49	-0.1505686231576462
1105	50-51	-0.12432471190771821
1105	52-53	-0.13289159209429613
1105	54-55	-0.20355781682513552
1105	56-57	-0.14707084980326357
1105	58-59	-0.11089567609738893
1105	60-61	-0.042275693303786
1105	62-63	0.006440709618281915
1105	64-65	-0.11432521722815636
1105	66-67	-0.10766688794587154
1105	68-69	-0.10282700375718434
1105	70-71	-0.045674514263055244
1105	72-73	0.0795046630452454
1105	74-75	0.10029192049932689
1105	76-77	0.03918940327739051
1105	78-79	-0.09375604762901446
1105	80-81	0.05255014845267425
1105	82-83	-0.05618390624880476
1105	84-85	-0.06451299739403282
1105	86-87	0.015905898908329164
1105	88-89	0.2065716830865867
1105	90-91	0.261449818790922
1105	92-93	0.006730243852405948
1105	94-95	-0.06269489681405105
1105	96-97	0.14145813682021213
1105	98-99	0.12782493316932886
1105	100	0.2872668803374925
1106	1	-0.19726402359820483
1106	2	-0.20226313347751557
1106	3	-0.1756145063699961
1106	4	-0.16890947697611125
1106	5	-0.2526914196936829
1106	6	-0.26434850159229484
1106	7	-0.23982605498126475
1106	8	-0.21839988812650546
1106	9	-0.23519661561502403
1106	10-11	-0.25491689476780977
1106	12-13	-0.29451076293992173
1106	14-15	-0.26272697281290647
1106	16-17	-0.29975385570789825
1106	18-19	-0.29026768585816143
1106	20-21	-0.22920811859520285
1106	22-23	-0.3614739930067472
1106	24-25	-0.3219692248463346
1106	26-27	-0.32779018617025457
1106	28-29	-0.3544122052318386
1106	30-31	-0.32457133870510546
1106	32-33	-0.3420641436798846
1106	34-35	-0.40342679823763206
1106	36-37	-0.46705082299706646
1106	38-39	-0.434548209669849
1106	40-41	-0.3034857571027416
1106	42-43	-0.31279098061679633
1106	44-45	-0.3465718628358445
1106	46-47	-0.3128312952551866
1106	48-49	-0.3101037334290311
1106	50-51	-0.32049235234834583
1106	52-53	-0.2817511817223206
1106	54-55	-0.35082552211845197
1106	56-57	-0.4147932643202843
1106	58-59	-0.28327728084207493
1106	60-61	-0.1394551070295762
1106	62-63	-0.135978376012023
1106	64-65	-0.23373839458449552
1106	66-67	-0.23731655150316655
1106	68-69	-0.24969964721033477
1106	70-71	-0.1813337765196401
1106	72-73	-0.10714124043206041
1106	74-75	-0.05084907633561997
1106	76-77	-0.09343761779080495
1106	78-79	-0.14763397801946
1106	80-81	0.10668698677642041
1106	82-83	0.027580317693708167
1106	84-85	-0.09311600165652578
1106	86-87	-0.17061806391803458
1106	88-89	-0.0791085074242659
1106	90-91	0.07497478819436054
1106	92-93	-0.16572688216151832
1106	94-95	-0.03935404488478511
1106	96-97	0.08751262156587458
1106	98-99	0.30006208722138794
1106	100	0.4623391812181179
1107	1	-0.3099979237210917
1107	2	-0.3231406936181287
1107	3	-0.3229357675644593
1107	4	-0.25938413916319547
1107	5	-0.3294905884214927
1107	6	-0.32869698608975995
1107	7	-0.36991251068211284
1107	8	-0.4690284758319052
1107	9	-0.5320487405464362
1107	10-11	-0.4389433216439258
1107	12-13	-0.3730794755293232
1107	14-15	-0.4742488869541006
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2207	72-73	0.868368122682849
2207	74-75	0.7906187588949045
2207	76-77	0.7007998102423407
2207	78-79	0.7497324197705808
2207	80-81	0.7114997148255995
2207	82-83	0.9182548869390068
2207	84-85	1.020178657171499
2207	86-87	1.152296015673194
2207	88-89	1.2180615519974864
2207	90-91	1.0948948713402231
2207	92-93	1.3053812135081593
2207	94-95	1.3731683125970484
2207	96-97	1.1557108092667292
2207	98-99	1.1456010978467255
2207	100	1.0482105318670119
2208	1	-0.2675557154475925
2208	2	-0.26127960615587753
2208	3	-0.2541783934219026
2208	4	-0.24311484108343961
2208	5	-0.3161703300949412
2208	6	-0.295271608003965
2208	7	-0.2709606331737646
2208	8	-0.24697635311400745
2208	9	-0.325432269187786
2208	10-11	-0.27256818409534134
2208	12-13	-0.22946440210282049
2208	14-15	-0.2956813124755371
2208	16-17	-0.290556094771226
2208	18-19	-0.34895430561977037
2208	20-21	-0.29465626121361055
2208	22-23	-0.347086414555271
2208	24-25	-0.3274710370447593
2208	26-27	-0.29634890695155036
2208	28-29	-0.3613611069164122
2208	30-31	-0.32529421877332965
2208	32-33	-0.19467499927282006
2208	34-35	-0.1992259204941078
2208	36-37	-0.15594769266441944
2208	38-39	-0.17840172515182928
2208	40-41	-0.1781055271334111
2208	42-43	-0.1471742108982994
2208	44-45	-0.1703637030265952
2208	46-47	-0.09009162616897726
2208	48-49	0.01249243907004427
2208	50-51	-0.10370171773702808
2208	52-53	0.06799611997730892
2208	54-55	0.11724638845074153
2208	56-57	0.22942683793718643
2208	58-59	0.29563335042842454
2208	60-61	0.26288580300994724
2208	62-63	0.2973452129547809
2208	64-65	0.21515357439286475
2208	66-67	0.15767457562787612
2208	68-69	0.32145885519237893
2208	70-71	0.5565294983906384
2208	72-73	0.45911246317733045
2208	74-75	0.4559042887457032
2208	76-77	0.2591554633022106
2208	78-79	0.038969367198628646
2208	80-81	0.12018080628311623
2208	82-83	0.14644791775584665
2208	84-85	0.17913717325937029
2208	86-87	0.015696220068829092
2208	88-89	-0.16951576964589776
2208	90-91	-0.7673641460308644
2208	92-93	-2.138597742287457
2208	94-95	-2.3133836886514842
2208	96-97	-2.3016552761448263
2208	98-99	-2.802987969364473
2208	100	-3.200272591185332
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	479822.0
3	204197.0
4	204783.0
5	167682.0
6	153453.0
7	157471.0
8	165436.0
9	186075.0
10	215746.0
11	245846.0
12	277360.0
13	327160.0
14	407925.0
15	519438.0
16	626389.0
17	631593.0
18	584879.0
19	545308.0
20	551085.0
21	574047.0
22	602532.0
23	638970.0
24	678427.0
25	726858.0
26	785732.0
27	863535.0
28	964920.0
29	1102455.0
30	1272683.0
31	1480481.0
32	1755444.0
33	2152338.0
34	2784511.0
35	3797836.0
36	4785752.0
37	3929689.0
38	1109822.0
39	36277.0
40	12.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.87584508467733	27.935964434187404	27.850702318285848	22.337488162849414
2	23.54043017557458	27.841090300677024	26.980957185486677	21.637522338261718
3	23.524285305022715	26.31170854407211	26.809068595281687	23.354937555623483
4	24.462025344053274	25.791464788431462	26.55089958678506	23.195610280730204
5	24.571504259951766	26.202005192995802	26.259372343577148	22.96711820347528
6	24.618144050964634	26.044038101384213	25.67981189131207	23.658005956339075
7	24.544965626020456	26.37411842566198	25.927280401727167	23.153635546590397
8	24.038899840052153	26.228719155396867	26.196031908900856	23.536349095650124
9	23.963720851517035	25.95459642302018	26.318287242219768	23.763395483243016
10-11	23.79094687940618	26.165904005206208	26.316150218360512	23.726998897027094
12-13	23.530876771235835	26.21251010556527	26.496199718795854	23.76041340440304
14-15	23.176247179741903	26.516966843300686	27.017055320650062	23.289730656307345
16-17	22.987225829806793	26.40437403123964	27.232568452365502	23.375831686588064
18-19	22.57565198089105	26.37134640961773	27.978624692033122	23.074376917458096
20-21	22.25178761000736	26.097224961349298	28.82000576866634	22.830981659977002
22-23	22.287615711879294	26.027441910027143	29.2059317846671	22.47901059342647
24-25	22.211255353729385	25.897606179125603	29.629207714412097	22.261930752732916
26-27	22.2294930639345	25.951554773654294	29.64845824763697	22.17049391477423
28-29	22.308572060206433	25.995439676244704	29.606245574535222	22.08974268901364
30-31	22.29704401305807	25.924293187322174	29.570760954632103	22.207901844987656
32-33	22.273875832780366	26.0338407759604	29.46448102145189	22.227802369807346
34-35	22.37592877094439	26.040692483025975	29.49914637939171	22.084232366637927
36-37	22.402211402928568	26.012292999659987	29.36481072196086	22.220684875450587
38-39	22.430361481415027	26.121285200999438	29.19655601848257	22.251797299102964
40-41	22.519130314598357	26.259776569845805	28.95148138604164	22.269611729514196
42-43	22.67392958727322	26.316359052529847	28.52837027944669	22.48134108075024
44-45	22.72381883018758	26.55178763651958	28.074614602402498	22.649778930890342
46-47	23.009926228165337	26.687092308767106	27.61124024225763	22.691741220809927
48-49	23.079214089626873	26.72932036989173	27.2593072765817	22.93215826389969
50-51	23.127475330210157	26.800252757178544	27.045298806003988	23.026973106607315
52-53	23.282493254213147	26.838616207300277	26.885040013330986	22.993850525155594
54-55	23.357472331448406	26.781957464945993	26.794484126513694	23.066086077091907
56-57	23.332139440098366	26.855408537342708	26.746821710633938	23.06563031192499
58-59	23.428910678582422	26.91842220588904	26.668611241178642	22.98405587434989
60-61	23.412997583037193	26.880087603148795	26.61868442930612	23.08823038450789
62-63	23.43874936155193	26.973993125732456	26.549944510444927	23.037313002270682
64-65	23.508719560111395	27.041242037010115	26.47783629826666	22.972202104611828
66-67	23.5132232809871	27.044984467145945	26.349153485855886	23.09263876601107
68-69	23.516282418477534	27.15236787491106	26.265944108585437	23.065405598025972
70-71	23.61106792140625	27.201279326146487	26.21995033321724	22.967702419230022
72-73	23.587459036088905	27.24913772182501	26.021027447188246	23.14237579489784
74-75	23.648027914445915	27.360691084548016	25.959870803621378	23.031410197384687
76-77	23.17340071079611	27.721463938981984	25.752702838499292	23.352432511722615
78-79	23.443560807549268	27.628969058360582	25.6999709982687	23.22749913582145
80-81	23.391691302825528	27.747421333759565	25.55083228791966	23.31005507549525
82-83	23.39915125786443	27.875256524172347	25.418842535462055	23.30674968250117
84-85	23.348934634815706	27.828497080680354	25.336502796539488	23.486065487964453
86-87	23.252563476055148	28.003116707431534	25.193273784629433	23.551046031883878
88-89	23.450116005961057	28.025396833183407	25.005057141784544	23.519430019070995
90-91	23.201646762315832	28.128890641530376	24.99194959805221	23.677512998101587
92-93	23.1713356892108	28.28264076153483	24.887896965662236	23.658126583592136
94-95	23.255397238727067	28.27507527980683	24.706483630493178	23.763043850972924
96-97	23.154761385637787	28.362693176466486	24.45211115059915	24.030434287296575
98-99	23.13143199036158	28.386317152584766	24.370040491479173	24.112210365574484
100	23.994588660086993	28.289730704353094	23.751604345369177	23.964076290190732
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2343.0
1	1757.5
2	1051.5
3	998.0
4	1100.5
5	1309.5
6	1605.5
7	1947.5
8	2432.0
9	2962.0
10	3643.0
11	4569.5
12	5770.0
13	7671.5
14	12258.0
15	17340.5
16	23945.5
17	27856.5
18	28598.5
19	33788.5
20	53619.0
21	65103.0
22	66040.5
23	79333.0
24	95706.0
25	114262.0
26	138097.0
27	171393.5
28	210256.5
29	247855.0
30	286050.5
31	337771.0
32	404155.5
33	475894.5
34	553931.5
35	644139.0
36	748787.0
37	872918.0
38	996524.0
39	1095435.5
40	1175056.0
41	1278324.5
42	1449350.0
43	1613265.5
44	1708755.0
45	1765682.0
46	1755429.0
47	1680976.0
48	1602080.5
49	1557944.0
50	1508041.0
51	1404354.0
52	1262356.0
53	1107264.0
54	981481.5
55	885818.5
56	786500.0
57	690683.0
58	620905.0
59	560974.0
60	494030.5
61	428044.5
62	372971.5
63	328494.5
64	289578.0
65	253226.5
66	219491.0
67	187464.5
68	157547.0
69	131761.0
70	110274.0
71	91909.5
72	76527.0
73	63756.0
74	53012.5
75	43452.5
76	35295.0
77	28449.5
78	22902.0
79	18124.0
80	14004.5
81	10798.5
82	8207.0
83	5979.5
84	4308.0
85	3059.5
86	2169.5
87	1544.0
88	1076.5
89	742.0
90	536.5
91	368.0
92	227.0
93	146.5
94	93.0
95	55.5
96	30.0
97	14.5
98	6.5
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060903741429552086
2	0.0066468688628368335
3	0.0022701278240029035
4	6.349817322841255E-4
5	0.00610182016559724
6	7.875953675112116E-4
7	0.0021992714933617564
8	0.002068459806024254
9	4.46939931736466E-4
10-11	0.0023559729938181393
12-13	0.0019008573316230794
14-15	0.002621684233722441
16-17	0.003094513978577787
18-19	0.0018981320881368815
20-21	0.0024867846811556416
22-23	0.0012508867601648652
24-25	0.0016951014484151332
26-27	5.98190945220453E-4
28-29	2.2619520935443095E-4
30-31	9.156818113625158E-4
32-33	0.0010778337987912944
34-35	0.002324632693726863
36-37	4.319510925623772E-4
38-39	4.360389577916742E-4
40-41	8.434628589782697E-4
42-43	5.191588841207121E-4
44-45	6.540584366875112E-4
46-47	6.063666756790469E-4
48-49	0.0011473275076893427
50-51	6.908492237511837E-4
52-53	0.0013912367997040605
54-55	6.377069757703234E-4
56-57	7.944084762267064E-4
58-59	0.0011541406164048374
60-61	0.003335698027106307
62-63	0.0040279098726005895
64-65	0.0038344175850805345
66-67	0.004309972573422079
68-69	0.0024786089506970478
70-71	0.005014448014604253
72-73	0.005637166151200488
74-75	0.008182543567309386
76-77	0.00970186681086475
78-79	0.008328344093820977
80-81	0.008585879603266684
82-83	0.00679948249806392
84-85	0.0064642775492615686
86-87	0.00556630982055934
88-89	0.002211535089049647
90-91	4.060612794434966E-4
92-93	0.001343545038695596
94-95	0.0014443790476849207
96-97	0.005802043382115464
98-99	0.0022265239282237364
100	0.0017823092399734683
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.6693969E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.0787061744181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.53970487863259	27.614535229928617
2	12.365979187395165	8.922970593219995
3	4.484447102460773	4.8537914809320855
4	2.0353776850035694	2.9373517380484446
5	1.1068532017578734	1.996691572221812
6	0.6328273206294992	1.3698954576081586
7	0.3985668349195467	1.006584300955109
8	0.291583972637296	0.8415977979160457
9	0.23391512955730434	0.7595419706144034
>10	1.5331832519809299	11.206474877956927
>50	0.17038169462670325	4.250971864094615
>100	0.13367886968648537	11.503486486730761
>500	0.05799773849339472	14.756757587179273
>1k	0.015344408598050861	7.119977661087764
>5k	6.0466141362549745E-5	0.14072297409553142
>10k+	9.8257479454098E-5	0.7186484074104241
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40709	0.11094193707963289	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7252434861979636E-6	0.0	0.0	0.0	0.0
2	2.7252434861979636E-6	0.0	0.0	0.0	0.0
3	2.7252434861979636E-6	0.0	0.0	0.0	0.0
4	2.7252434861979636E-6	0.0	0.0	0.0	0.0
5	8.175730458593891E-6	0.0	0.0	0.0	0.0
6	1.0900973944791855E-5	0.0	0.0	0.0	0.0
7	1.0900973944791855E-5	0.0	0.0	2.7252434861979636E-6	0.0
8	1.0900973944791855E-5	2.7252434861979636E-6	0.0	2.7252434861979636E-6	2.7252434861979636E-6
9	2.180194788958371E-5	2.7252434861979636E-6	0.0	2.7252434861979636E-6	2.7252434861979636E-6
10-11	1.0219663073242363E-4	2.7252434861979636E-6	0.0	2.7252434861979636E-6	2.7252434861979636E-6
12-13	3.515564097195373E-4	2.7252434861979636E-6	0.0	2.7252434861979636E-6	2.7252434861979636E-6
14-15	9.879007637467617E-4	2.7252434861979636E-6	0.0	4.0878652292969454E-6	2.7252434861979636E-6
16-17	0.002178832167215272	2.7252434861979636E-6	0.0	8.175730458593891E-6	2.7252434861979636E-6
18-19	0.003995206950766214	2.7252434861979636E-6	2.7252434861979636E-6	1.0900973944791855E-5	5.450486972395927E-6
20-21	0.006278960992200108	2.7252434861979636E-6	2.7252434861979636E-6	1.0900973944791855E-5	5.450486972395927E-6
22-23	0.00920723511811982	2.7252434861979636E-6	2.7252434861979636E-6	1.0900973944791855E-5	5.450486972395927E-6
24-25	0.012874050228799179	5.450486972395927E-6	2.7252434861979636E-6	1.3626217430989818E-5	5.450486972395927E-6
26-27	0.017096815010662923	6.813108715494909E-6	2.7252434861979636E-6	1.7714082660286764E-5	5.450486972395927E-6
28-29	0.021352282714361043	8.175730458593891E-6	2.7252434861979636E-6	1.9076704403385744E-5	8.175730458593891E-6
30-31	0.024784726885227377	8.175730458593891E-6	2.7252434861979636E-6	2.0439326146484726E-5	8.175730458593891E-6
32-33	0.027785219963531338	8.175730458593891E-6	2.7252434861979636E-6	2.9977678348177598E-5	1.0900973944791855E-5
34-35	0.030934238811833083	8.175730458593891E-6	2.7252434861979636E-6	3.2702921834375564E-5	1.0900973944791855E-5
36-37	0.034280837812884185	8.175730458593891E-6	4.0878652292969454E-6	3.2702921834375564E-5	1.0900973944791855E-5
38-39	0.037155969690823035	8.175730458593891E-6	5.450486972395927E-6	3.2702921834375564E-5	1.0900973944791855E-5
40-41	0.03963866650674938	8.175730458593891E-6	6.813108715494909E-6	3.542816532057352E-5	1.2263595687890837E-5
42-43	0.04249472168028484	8.175730458593891E-6	1.0900973944791855E-5	3.67907870636725E-5	1.3626217430989818E-5
44-45	0.04565055363730208	9.538352201692872E-6	1.3626217430989818E-5	3.9516030549870473E-5	1.3626217430989818E-5
46-47	0.04931600612623835	1.0900973944791855E-5	1.4988839174088799E-5	4.087865229296945E-5	1.3626217430989818E-5
48-49	0.05337798154241641	1.0900973944791855E-5	1.6351460917187782E-5	4.087865229296945E-5	1.6351460917187782E-5
50-51	0.05790597359473433	1.0900973944791855E-5	1.7714082660286764E-5	4.087865229296945E-5	1.6351460917187782E-5
52-53	0.06313844108823441	1.0900973944791855E-5	1.9076704403385744E-5	4.087865229296945E-5	1.6351460917187782E-5
54-55	0.06952232395465315	1.0900973944791855E-5	2.180194788958371E-5	4.087865229296945E-5	1.6351460917187782E-5
56-57	0.07738601403407737	1.0900973944791855E-5	2.452719137578167E-5	4.360389577916742E-5	1.6351460917187782E-5
58-59	0.08693662983145814	1.0900973944791855E-5	2.452719137578167E-5	4.6329139265365377E-5	1.9076704403385744E-5
60-61	0.09799839314193567	1.2263595687890837E-5	2.452719137578167E-5	4.905438275156334E-5	2.180194788958371E-5
62-63	0.11086699288376245	1.4988839174088799E-5	2.7252434861979636E-5	5.177962623776131E-5	2.452719137578167E-5
64-65	0.12691186390875295	1.6351460917187782E-5	2.7252434861979636E-5	5.450486972395927E-5	2.452719137578167E-5
66-67	0.14631150966525316	1.6351460917187782E-5	2.7252434861979636E-5	5.450486972395927E-5	2.7252434861979633E-5
68-69	0.16959735263307166	1.6351460917187782E-5	2.7252434861979636E-5	5.450486972395927E-5	2.9977678348177598E-5
70-71	0.19741663814018046	2.180194788958371E-5	2.7252434861979636E-5	5.9955356696355196E-5	2.9977678348177598E-5
72-73	0.23088399077243454	2.180194788958371E-5	2.9977678348177598E-5	6.67684654118501E-5	2.9977678348177598E-5
74-75	0.27061122769248536	2.452719137578167E-5	2.9977678348177598E-5	6.949370889804807E-5	2.9977678348177598E-5
76-77	0.31779064292554454	2.452719137578167E-5	3.2702921834375564E-5	7.630681761354297E-5	3.1340300091276584E-5
78-79	0.37245902725867563	3.1340300091276584E-5	3.67907870636725E-5	8.720779155833484E-5	3.2702921834375564E-5
80-81	0.434659984587658	3.815340880677149E-5	4.087865229296945E-5	9.265827853073075E-5	3.2702921834375564E-5
82-83	0.5061949008568684	4.087865229296945E-5	4.49665175222664E-5	9.402090027382974E-5	3.406554357747454E-5
84-85	0.5874739252109795	4.769176100846436E-5	5.177962623776131E-5	1.0083400898932464E-4	3.815340880677149E-5
86-87	0.6793173559393371	5.041700449466232E-5	5.450486972395927E-5	1.049218742186216E-4	3.9516030549870473E-5
88	0.7553938904782963	5.450486972395927E-5	5.723011321015723E-5	1.0900973944791855E-4	4.087865229296945E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	23865	0.0	48.37943	1
TCTCGAG	28965	0.0	39.773804	2
CTCGAGG	38110	0.0	30.11699	3
GATCCGG	40545	0.0	29.27674	1
TCGAGGG	45070	0.0	25.37231	4
ATCCGGC	51150	0.0	23.322405	2
TATCTCG	15395	0.0	20.486614	2
TCCGGCG	61955	0.0	19.436033	3
GCGCCGT	66740	0.0	17.61236	9
GGATCCG	26595	0.0	17.426264	2
CGAGGGC	67445	0.0	17.254635	5
ATATCTC	19200	0.0	17.097195	1
CGGCGCG	70475	0.0	16.819569	5
AGGGCGC	70875	0.0	16.14702	7
TGGATCC	29985	0.0	15.982356	1
ATGGATC	25395	0.0	15.667253	1
CCGGCGC	80550	0.0	14.937568	4
CGTCGAC	64085	0.0	14.792554	12-13
GCCGTCG	66625	0.0	14.369662	10-11
TCGACTT	66275	0.0	14.36761	14-15
>>END_MODULE