FastQCFastQC Report
Wed 25 May 2016
SRR491011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33286654
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1095120.32899672042735206No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT885640.2660645915326906No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT716360.21520937490442865No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA518920.15589431127562414No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT451000.1354897371180654No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437660.13148212493812084No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367860.11051275985865085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG360000.058.5584682
ATATCTC380900.056.61051
ATCTCGA484700.043.122083
ATATGGA383100.042.8923031
TATGGAT393150.041.569632
TCTCGAG539850.038.1508524
ATGGATC452300.036.204053
TGGATCC513500.031.7060684
CTCGAGG651500.031.468565
GGATCCG509050.030.9491845
GATCCGG764450.030.3603441
TCGAGGG783100.026.262996
ATCCGGC926650.024.9714552
TCCGGCG1081950.021.3294623
CGAGGGC996450.020.4701127
CCGGCGC1253700.018.3586794
GCGCCGT1251100.018.2835529
CGGCGCG1300450.017.6445145
AGGGCGC1257050.016.1294259
GAGGGCG1269750.016.0011468
Produced by FastQC (version 0.11.5)