FastQCFastQC Report
Wed 25 May 2016
SRR491010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21392946
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG103700.038.5728032
ATCTCGA137800.032.2878721
ATATCTC134550.030.1315211
GATCCGG239900.027.0941331
TCTCGAG172250.025.8417032
ATCCGGC301000.021.6903292
CTCGAGG213200.020.8328483
GGATCCG161600.019.806735
ATGGATC169850.019.7577133
TATGGAT178200.018.9913222
TCCGGCG375850.017.4822223
TGGATCC191400.017.164984
ATATGGA200100.017.0877481
TCGAGGG259100.017.0698724
GCGCCGT388150.016.7221479
CGTCGAC372550.015.61819912-13
CGGCGCG425750.015.1904565
AGGGCGC423700.014.976342
GCCGTCG392600.014.9104710-11
TCGACTT391850.014.90302814-15
Produced by FastQC (version 0.11.5)