##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR491010_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21392946 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72716829182853 33.0 31.0 34.0 30.0 34.0 2 32.00031636596474 34.0 31.0 34.0 30.0 34.0 3 32.07170321469516 34.0 31.0 34.0 30.0 34.0 4 35.44256513338556 37.0 35.0 37.0 33.0 37.0 5 35.21763346665765 37.0 35.0 37.0 32.0 37.0 6 35.17128753562039 37.0 35.0 37.0 32.0 37.0 7 35.106184253445036 37.0 35.0 37.0 32.0 37.0 8 35.02208957102028 37.0 35.0 37.0 32.0 37.0 9 36.57294497915341 39.0 37.0 39.0 32.0 39.0 10-11 36.42776455379264 39.0 37.0 39.0 32.0 39.0 12-13 36.223697544975806 39.0 37.0 39.0 32.0 39.0 14-15 37.51360607370299 40.0 38.0 41.0 32.0 41.0 16-17 37.459102780888614 40.0 38.0 41.0 32.0 41.0 18-19 37.35112756326314 40.0 38.0 41.0 32.0 41.0 20-21 37.17931300812894 40.0 38.0 41.0 31.0 41.0 22-23 37.024977392080544 40.0 38.0 41.0 31.0 41.0 24-25 36.835073696722276 40.0 37.5 41.0 30.0 41.0 26-27 36.50682362775095 40.0 37.0 41.0 30.0 41.0 28-29 36.18351254661233 40.0 36.0 41.0 29.0 41.0 30-31 35.91110642264978 40.0 36.0 41.0 27.5 41.0 32-33 35.67252857554074 40.0 35.0 41.0 27.0 41.0 34-35 35.38499178654497 39.0 35.0 41.0 25.5 41.0 36-37 35.0793440276996 39.0 35.0 41.0 24.5 41.0 38-39 34.68153163664322 39.0 35.0 40.0 22.5 41.0 40-41 34.216564329195236 38.5 34.5 40.0 18.0 41.0 42-43 33.74157741061002 38.0 33.5 40.0 12.0 41.0 44-45 33.2947893899232 38.0 33.0 40.0 7.0 41.0 46-47 32.75426579864222 38.0 33.0 40.0 2.0 41.0 48-49 32.2566227905217 38.0 32.0 40.0 2.0 41.0 50-51 31.71986272951841 37.5 31.0 40.0 2.0 41.0 52-53 31.194335833877204 37.0 30.5 40.0 2.0 41.0 54-55 30.60753998070205 36.5 29.5 39.0 2.0 40.5 56-57 30.060282020063998 36.0 28.0 39.0 2.0 40.0 58-59 29.49352221054548 35.0 27.5 39.0 2.0 40.0 60-61 28.875423515770105 35.0 26.0 38.0 2.0 40.0 62-63 28.206864332757164 34.0 25.5 38.0 2.0 40.0 64-65 27.590027946595107 34.0 23.0 38.0 2.0 40.0 66-67 26.870459239227735 33.5 21.0 37.0 2.0 39.0 68-69 26.182168388589396 33.0 18.5 36.0 2.0 39.0 70-71 25.465449756195337 32.0 11.0 36.0 2.0 38.5 72-73 24.69465196612005 31.0 7.0 35.0 2.0 38.0 74-75 23.99380370520264 31.0 2.0 35.0 2.0 37.0 76-77 22.989997567422456 29.5 2.0 34.0 2.0 36.0 78-79 23.09872163001767 30.0 2.0 34.0 2.0 36.0 80-81 22.564206421125917 30.0 2.0 34.0 2.0 36.0 82-83 21.896479311451543 29.5 2.0 34.0 2.0 35.0 84-85 21.040523521164406 29.0 2.0 34.0 2.0 35.0 86-87 20.198905120407446 27.0 2.0 33.0 2.0 35.0 88-89 19.44680833111999 26.0 2.0 33.0 2.0 34.5 90-91 18.655039189085972 25.0 2.0 32.0 2.0 34.0 92-93 17.880456436434702 23.5 2.0 32.0 2.0 34.0 94-95 16.858333139344154 19.0 2.0 31.5 2.0 34.0 96-97 15.8312637726473 6.5 2.0 31.0 2.0 34.0 98-99 14.973588840919806 2.0 2.0 31.0 2.0 34.0 100 14.267916302878529 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21165418931295932 1101 2 0.2535437516300689 1101 3 0.27104487548866985 1101 4 0.24247962635452325 1101 5 0.2653355539811386 1101 6 0.24442375839206676 1101 7 0.22436854243530746 1101 8 0.23284843441327752 1101 9 0.2887613362906265 1101 10-11 0.30771111305608656 1101 12-13 0.2762666838318353 1101 14-15 0.36502600095067805 1101 16-17 0.34483592326438384 1101 18-19 0.3632615478802137 1101 20-21 0.3852551605503223 1101 22-23 0.31554952871403685 1101 24-25 0.31113720969295144 1101 26-27 0.2626776023798527 1101 28-29 0.2389976691107094 1101 30-31 0.25232106767247586 1101 32-33 0.2464399224423417 1101 34-35 0.18903303681624095 1101 36-37 0.1429319315222699 1101 38-39 0.19670925744670598 1101 40-41 0.11237266452271655 1101 42-43 -0.0011049009534573884 1101 44-45 -0.020177883842855238 1101 46-47 -0.12276964001785018 1101 48-49 -0.16910223066172136 1101 50-51 -0.18723120659209513 1101 52-53 -0.19490157251589935 1101 54-55 -0.24750785435453793 1101 56-57 -0.31626610295032265 1101 58-59 -0.29003035104506125 1101 60-61 -0.41373574104989785 1101 62-63 -0.3534135333478474 1101 64-65 -0.4418675803852814 1101 66-67 -0.4355523972593893 1101 68-69 -0.6488262303330359 1101 70-71 -0.7141688042781951 1101 72-73 -0.6458333219402732 1101 74-75 -0.7031662059407466 1101 76-77 -0.7056556994858454 1101 78-79 -0.8493012415380186 1101 80-81 -0.7008941945001936 1101 82-83 -0.9231636573172715 1101 84-85 -1.208589352292634 1101 86-87 -1.1445954885268748 1101 88-89 -1.050546321812316 1101 90-91 -0.7479382084010346 1101 92-93 -0.9091996981260166 1101 94-95 -0.8843095198448125 1101 96-97 -0.8567909034185437 1101 98-99 -0.7217946597841554 1101 100 -0.39944785384226833 1102 1 0.1228353888398459 1102 2 0.062380003705087006 1102 3 0.10878906240333919 1102 4 0.10290127968261231 1102 5 0.14755807801305565 1102 6 0.1620576277129615 1102 7 0.1491535903305845 1102 8 0.15485572234359068 1102 9 0.16269433403412137 1102 10-11 0.1633813123488821 1102 12-13 0.13756201890901565 1102 14-15 0.21132287992007548 1102 16-17 0.18511444454809833 1102 18-19 0.22296114541863687 1102 20-21 0.23694972271309922 1102 22-23 0.19169598741911642 1102 24-25 0.20031976843751664 1102 26-27 0.16318649661110385 1102 28-29 0.09456082270602195 1102 30-31 0.13983789755932463 1102 32-33 0.10310276644597849 1102 34-35 0.10316878502619176 1102 36-37 0.11004386318043657 1102 38-39 0.07808803446247481 1102 40-41 0.10598046774359204 1102 42-43 0.11987771811614323 1102 44-45 0.07641398795779963 1102 46-47 -0.040010111999443154 1102 48-49 -0.08813751254461977 1102 50-51 -0.052195180230526717 1102 52-53 -0.21158473993457605 1102 54-55 -0.23287604619193303 1102 56-57 -0.33513166794140403 1102 58-59 -0.3054087518813091 1102 60-61 -0.2887609506850808 1102 62-63 -0.25910222702013996 1102 64-65 -0.3164146315332026 1102 66-67 -0.23924697138151174 1102 68-69 -0.3444095723632259 1102 70-71 -0.41213215451130836 1102 72-73 -0.28662591415879035 1102 74-75 -0.37048886957471083 1102 76-77 -0.40681062797757406 1102 78-79 -0.5197234314228254 1102 80-81 -0.404872654930152 1102 82-83 -0.6573229967052718 1102 84-85 -0.9123282618478861 1102 86-87 -0.7913374490547405 1102 88-89 -0.6068749236699738 1102 90-91 -0.5054936138783965 1102 92-93 -0.6555029672326427 1102 94-95 -0.8062861459474213 1102 96-97 -0.8274590806260989 1102 98-99 -0.6567285426056326 1102 100 -0.3381107115701969 1103 1 0.024182787565550257 1103 2 0.024304218122068733 1103 3 0.012550527324108884 1103 4 -0.05204000303238843 1103 5 0.004501853212339313 1103 6 0.04641140312647707 1103 7 0.020059278056088203 1103 8 0.02360878606219785 1103 9 0.04029957913792259 1103 10-11 0.022932295936485048 1103 12-13 -0.040729781953295685 1103 14-15 -0.015864806710460755 1103 16-17 0.047642376710754775 1103 18-19 0.0658182857885663 1103 20-21 -0.021133179875654662 1103 22-23 -0.004736086484413704 1103 24-25 0.10261932459558665 1103 26-27 0.05824874217364595 1103 28-29 0.027579958310496977 1103 30-31 0.06527781492555818 1103 32-33 0.02973415629042364 1103 34-35 -0.08347084093248469 1103 36-37 -0.11122544764501185 1103 38-39 -0.2746441980548511 1103 40-41 -0.11268782028664504 1103 42-43 -0.26266452181187105 1103 44-45 -0.23023180834702828 1103 46-47 -0.29056041264399113 1103 48-49 -0.2564065575764829 1103 50-51 -0.5524617331058224 1103 52-53 -0.4087007547571737 1103 54-55 -0.3621943736834403 1103 56-57 -0.3399716373612591 1103 58-59 -0.6879464805983808 1103 60-61 -0.4551915625528693 1103 62-63 -0.3367022217869433 1103 64-65 -0.47363843326855815 1103 66-67 -0.4080446621099547 1103 68-69 -0.43209651562778006 1103 70-71 -0.49074507870071216 1103 72-73 -0.26248464772619684 1103 74-75 -0.2952023386426248 1103 76-77 -0.284119803756667 1103 78-79 -0.34390497432859846 1103 80-81 -0.17378595306667677 1103 82-83 -0.35552101318302576 1103 84-85 -0.5430769141313405 1103 86-87 -0.555212140176252 1103 88-89 -0.24179775271067072 1103 90-91 -0.3082920483094327 1103 92-93 -0.46271028897685795 1103 94-95 -0.511022549348052 1103 96-97 -0.5584506362172075 1103 98-99 -0.5041691641270916 1103 100 -0.34596410213598894 1104 1 -0.024567593182844405 1104 2 -0.030752754158200446 1104 3 -0.01026736558706176 1104 4 -0.043730963936880585 1104 5 -0.007191690468943079 1104 6 -0.021547028400519253 1104 7 -0.023150084286278627 1104 8 -0.02187838399483155 1104 9 -0.06044609842840032 1104 10-11 -0.04903674767405164 1104 12-13 -0.038582935033637966 1104 14-15 -0.019604219769533415 1104 16-17 -0.026674368811050897 1104 18-19 0.006484433980240567 1104 20-21 0.040640025123956036 1104 22-23 0.007667731215072138 1104 24-25 -8.264791377712299E-4 1104 26-27 0.03848181434882747 1104 28-29 -0.001973672735040566 1104 30-31 8.123933701114083E-4 1104 32-33 0.0352342424246288 1104 34-35 0.028578474152489264 1104 36-37 0.03842929557671937 1104 38-39 0.023277597956059992 1104 40-41 0.07115888230024581 1104 42-43 0.03289855666981367 1104 44-45 0.020518012653134576 1104 46-47 -0.013050183196540388 1104 48-49 0.021206360248925193 1104 50-51 0.04204889633638231 1104 52-53 0.07743036934682479 1104 54-55 0.03299813175849664 1104 56-57 0.06874842826811545 1104 58-59 0.023128827662873164 1104 60-61 0.11333606602573809 1104 62-63 0.22145156009946376 1104 64-65 0.12464142673946199 1104 66-67 0.08125148504397472 1104 68-69 0.09571336688016174 1104 70-71 0.014664378714130066 1104 72-73 0.11283799302337272 1104 74-75 0.14544548824681414 1104 76-77 0.08739328124763546 1104 78-79 -0.016443605700480646 1104 80-81 0.1288153306583375 1104 82-83 0.013631561617170718 1104 84-85 -0.1456108855547278 1104 86-87 -0.014114751309005413 1104 88-89 0.19102317632946253 1104 90-91 0.21525866119271697 1104 92-93 0.047138226424088714 1104 94-95 0.06681278926741285 1104 96-97 0.18511831938460155 1104 98-99 0.34662132143638047 1104 100 0.5321938478636348 1105 1 -0.08301726351480099 1105 2 -0.09270858049065112 1105 3 -0.07877520731444321 1105 4 -0.05970303312678027 1105 5 -0.0816427818941321 1105 6 -0.11084099678735271 1105 7 -0.07850767042675244 1105 8 -0.0650739237024851 1105 9 -0.15103595707181938 1105 10-11 -0.09649060626453831 1105 12-13 -0.08854905998648377 1105 14-15 -0.19248394555404502 1105 16-17 -0.15136754320598556 1105 18-19 -0.1527173790322962 1105 20-21 -0.1801373948900178 1105 22-23 -0.19690451995422364 1105 24-25 -0.16341170253648585 1105 26-27 -0.1093082742272955 1105 28-29 -0.11189375054012629 1105 30-31 -0.07522664492646669 1105 32-33 -0.1298432226515942 1105 34-35 -0.148056908911677 1105 36-37 -0.16445443927980818 1105 38-39 -0.10674760718757881 1105 40-41 -0.06881398900077329 1105 42-43 -0.130943922055053 1105 44-45 -0.07552548979179363 1105 46-47 -0.02616806071871025 1105 48-49 -0.15897211774478137 1105 50-51 -0.16396435974047563 1105 52-53 -0.15647450256377127 1105 54-55 -0.24560837548851921 1105 56-57 -0.1935913768177322 1105 58-59 -0.12078789899120324 1105 60-61 -0.020915114026490045 1105 62-63 0.009628706367511342 1105 64-65 -0.09600155967597956 1105 66-67 -0.08697855209883087 1105 68-69 -0.12225467624298503 1105 70-71 -0.07579360027589388 1105 72-73 0.036586625643959536 1105 74-75 0.07027440272635488 1105 76-77 0.03089373978195198 1105 78-79 -0.09962503011860235 1105 80-81 0.03103331789597874 1105 82-83 -0.06293511271574914 1105 84-85 -0.08425467478203785 1105 86-87 -0.07599454892657675 1105 88-89 0.16333933818382818 1105 90-91 0.2135652645752053 1105 92-93 -6.483252647342397E-4 1105 94-95 -0.09009745757238008 1105 96-97 0.11975580991592949 1105 98-99 0.16500715696565216 1105 100 0.31530822072662623 1106 1 -0.2093648254370457 1106 2 -0.20424665821810706 1106 3 -0.17637343156286178 1106 4 -0.15609717516399968 1106 5 -0.2637952138785735 1106 6 -0.2865388574698926 1106 7 -0.2654240719192771 1106 8 -0.23118409490415104 1106 9 -0.27609014543507726 1106 10-11 -0.2575324015279392 1106 12-13 -0.3003475866627028 1106 14-15 -0.2726634629508382 1106 16-17 -0.3253523041083426 1106 18-19 -0.31973187677829173 1106 20-21 -0.23848514880909732 1106 22-23 -0.35766208493647156 1106 24-25 -0.339275960772909 1106 26-27 -0.33022155802483155 1106 28-29 -0.3460699923416328 1106 30-31 -0.30624804588676824 1106 32-33 -0.32919494653148007 1106 34-35 -0.40052308205159903 1106 36-37 -0.44984150373896625 1106 38-39 -0.4101567537521973 1106 40-41 -0.288177987635045 1106 42-43 -0.3104679952017264 1106 44-45 -0.3559173990373026 1106 46-47 -0.3085221632221362 1106 48-49 -0.26428293325527363 1106 50-51 -0.29853714660551844 1106 52-53 -0.2690133391400842 1106 54-55 -0.32401792418790976 1106 56-57 -0.39462215739829176 1106 58-59 -0.2535170752486309 1106 60-61 -0.1271330723539137 1106 62-63 -0.09087917583158855 1106 64-65 -0.16892904606470083 1106 66-67 -0.20277845866887745 1106 68-69 -0.18265195327156647 1106 70-71 -0.1405587312047416 1106 72-73 -0.056469785007962514 1106 74-75 -0.0023997600161855814 1106 76-77 -0.0582381603022597 1106 78-79 -0.12734832422077247 1106 80-81 0.10910429185236126 1106 82-83 0.04880685103784188 1106 84-85 -0.03702750914240127 1106 86-87 -0.13126049960489183 1106 88-89 -0.0593867201777627 1106 90-91 0.09190153997563044 1106 92-93 -0.19160287739471826 1106 94-95 -0.03388144685916572 1106 96-97 0.09196409117037341 1106 98-99 0.3382118803595695 1106 100 0.5278449348346825 1107 1 -0.28092399841414206 1107 2 -0.29500458916014694 1107 3 -0.2868068104473238 1107 4 -0.251171197414493 1107 5 -0.3064248000750567 1107 6 -0.30052641032941096 1107 7 -0.3275450841646119 1107 8 -0.41018978769695735 1107 9 -0.49198697423314286 1107 10-11 -0.4110381196544566 1107 12-13 -0.31960532017357224 1107 14-15 -0.4395749119508281 1107 16-17 -0.4474266116500445 1107 18-19 -0.4350019653220798 1107 20-21 -0.34596890282049486 1107 22-23 -0.41466245589379724 1107 24-25 -0.37225378118773733 1107 26-27 -0.4546968048809603 1107 28-29 -0.3831062759464672 1107 30-31 -0.39816083479588116 1107 32-33 -0.29580517928157946 1107 34-35 -0.3032927990407188 1107 36-37 -0.310281939615777 1107 38-39 -0.35696670500107786 1107 40-41 -0.296484130929656 1107 42-43 -0.34767271756393825 1107 44-45 -0.24614838659676508 1107 46-47 -0.25853956263368616 1107 48-49 -0.16415795710317838 1107 50-51 -0.008968257760891163 1107 52-53 0.011023835650892266 1107 54-55 0.027422772799805983 1107 56-57 0.12831540479668035 1107 58-59 0.22616019265787202 1107 60-61 0.21959355485360632 1107 62-63 0.11057780652306093 1107 64-65 0.16614850980210605 1107 66-67 0.19684582503304782 1107 68-69 0.2051505205109052 1107 70-71 0.15621271544669924 1107 72-73 0.11498614133038387 1107 74-75 0.22508672138125263 1107 76-77 0.3156273501385911 1107 78-79 0.24158858921319037 1107 80-81 0.42297682036142703 1107 82-83 0.30959276352156806 1107 84-85 0.1877225430954752 1107 86-87 0.23111073278882088 1107 88-89 0.2533262156057603 1107 90-91 0.3586495074002478 1107 92-93 0.21267996076904083 1107 94-95 0.15681209054181622 1107 96-97 0.30615009482462874 1107 98-99 0.571997480850241 1107 100 0.5137757112453869 1108 1 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0.8598763583039748 2204 92-93 0.8921664589093723 2204 94-95 0.9448915191144494 2204 96-97 0.9322945845697763 2204 98-99 1.0446428062275945 2204 100 1.2411760099348186 2205 1 0.09299166531693004 2205 2 0.07627496815297974 2205 3 0.07637083603579242 2205 4 0.07580706702820805 2205 5 0.10429963468425996 2205 6 0.09997352181952124 2205 7 0.11989710172443324 2205 8 0.10180784618161454 2205 9 0.13953322312796956 2205 10-11 0.09287524564550154 2205 12-13 0.10693231129219072 2205 14-15 0.18332718311609142 2205 16-17 0.18394493522438893 2205 18-19 0.14792806943916048 2205 20-21 0.17291541502357433 2205 22-23 0.17534706527330712 2205 24-25 0.16962460179972538 2205 26-27 0.17219365261506425 2205 28-29 0.19192446328020907 2205 30-31 0.14358776584468558 2205 32-33 0.11946580016427077 2205 34-35 0.18890114513303047 2205 36-37 0.16277583548167485 2205 38-39 0.1374009911146814 2205 40-41 0.18830025363924818 2205 42-43 0.2694732788641545 2205 44-45 0.1771406356892058 2205 46-47 0.2755651378011521 2205 48-49 0.2719517079896292 2205 50-51 0.2879240488459658 2205 52-53 0.303282367278225 2205 54-55 0.31825232337166653 2205 56-57 0.49854082455823345 2205 58-59 0.4157421390971976 2205 60-61 0.4468990862893705 2205 62-63 0.3458349409221171 2205 64-65 0.3447011577624153 2205 66-67 0.3451190897869729 2205 68-69 0.48145390159056234 2205 70-71 0.5622138328630051 2205 72-73 0.4728599431993672 2205 74-75 0.42957565428248756 2205 76-77 0.3154852663335177 2205 78-79 0.4005080572121251 2205 80-81 0.3308474555550589 2205 82-83 0.4626712781797515 2205 84-85 0.6207080455307903 2205 86-87 0.5159118635015787 2205 88-89 0.6165433512241769 2205 90-91 0.7021922796181777 2205 92-93 0.6630262056015042 2205 94-95 0.6134874159767953 2205 96-97 0.6838993474862232 2205 98-99 0.8258727892476809 2205 100 0.9792497152656328 2206 1 -0.07065480855491657 2206 2 -0.08604607544838316 2206 3 -0.06624445468752072 2206 4 -0.06445024073772032 2206 5 -0.058254546547949815 2206 6 -0.0591228546389857 2206 7 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-0.07711805316881382 2206 68-69 0.008437484126368133 2206 70-71 0.17376186710129815 2206 72-73 0.13706711699398966 2206 74-75 0.11547552739902756 2206 76-77 0.13587091577200994 2206 78-79 0.17903227207479233 2206 80-81 0.16058641481182434 2206 82-83 0.3306883504442091 2206 84-85 0.45338319177191266 2206 86-87 0.3795392974649694 2206 88-89 0.4612319620882026 2206 90-91 0.4901451323981796 2206 92-93 0.7930834749661742 2206 94-95 0.7978475164227454 2206 96-97 0.5725288603891325 2206 98-99 0.6129722814880516 2206 100 0.6592261986992067 2207 1 -0.13790353260944244 2207 2 -0.13937351489246197 2207 3 -0.13210186706743698 2207 4 -0.1074946124851337 2207 5 -0.14165129481562388 2207 6 -0.1574970041063679 2207 7 -0.13449149615029654 2207 8 -0.16094537361284011 2207 9 -0.13969808060323885 2207 10-11 -0.15024527259752318 2207 12-13 -0.13016302124404433 2207 14-15 -0.12077125158775459 2207 16-17 -0.15355494938922476 2207 18-19 -0.1414059535944716 2207 20-21 -0.08595799921983627 2207 22-23 -0.07416149457623789 2207 24-25 -0.07092000432061951 2207 26-27 -0.08060981728146999 2207 28-29 -0.09727770709562122 2207 30-31 -0.10371070706475649 2207 32-33 -0.12888557482498442 2207 34-35 -0.026958036316266032 2207 36-37 -0.03191600473162026 2207 38-39 0.06568648062764026 2207 40-41 0.10578350045637563 2207 42-43 0.18314937467721393 2207 44-45 0.04082931351437935 2207 46-47 0.18724455341843083 2207 48-49 0.23659694436988588 2207 50-51 0.20650917003845848 2207 52-53 0.30520685447255147 2207 54-55 0.4488161199340297 2207 56-57 0.5722302592943436 2207 58-59 0.6189363457650217 2207 60-61 0.6346461606832108 2207 62-63 0.5909169269780605 2207 64-65 0.6387409061506624 2207 66-67 0.5004739228462718 2207 68-69 0.6108098813718996 2207 70-71 0.785105901300966 2207 72-73 0.7647058647798737 2207 74-75 0.6888216208384605 2207 76-77 0.5929566045637173 2207 78-79 0.6653955339743547 2207 80-81 0.6120366289036667 2207 82-83 0.8334952038217409 2207 84-85 0.9531519708645533 2207 86-87 1.1005686054819357 2207 88-89 1.1600763538761925 2207 90-91 1.0384185982817549 2207 92-93 1.231500005097935 2207 94-95 1.2887971123362192 2207 96-97 1.0610011899312735 2207 98-99 1.083219291776123 2207 100 1.0348506885984907 2208 1 -0.26021929139005096 2208 2 -0.2511832187387171 2208 3 -0.251220514836227 2208 4 -0.2508338269621575 2208 5 -0.29890513981231237 2208 6 -0.27805476565369247 2208 7 -0.2563243452340842 2208 8 -0.24348678412091118 2208 9 -0.31136030014247496 2208 10-11 -0.29722420422812945 2208 12-13 -0.24846157430044258 2208 14-15 -0.29595773914157775 2208 16-17 -0.29857924966783145 2208 18-19 -0.35559909411097834 2208 20-21 -0.3097908584594151 2208 22-23 -0.36388816025498016 2208 24-25 -0.34974757085256414 2208 26-27 -0.28818059366658133 2208 28-29 -0.3374136467361737 2208 30-31 -0.31941800372432994 2208 32-33 -0.15765299767095087 2208 34-35 -0.17785015086739264 2208 36-37 -0.14166843993061207 2208 38-39 -0.16358999364388183 2208 40-41 -0.1477308432950437 2208 42-43 -0.10341712944428139 2208 44-45 -0.152212225384325 2208 46-47 -0.0717906536907904 2208 48-49 0.03210593001956852 2208 50-51 -0.08064681065913604 2208 52-53 0.07901954076933038 2208 54-55 0.11489074132246202 2208 56-57 0.18199918777034085 2208 58-59 0.23653760374235233 2208 60-61 0.2503102651466804 2208 62-63 0.2516157068612195 2208 64-65 0.1983566790318534 2208 66-67 0.15839762865302376 2208 68-69 0.32731822514003994 2208 70-71 0.5132236898335947 2208 72-73 0.44242774688246556 2208 74-75 0.46290936546803607 2208 76-77 0.3024665342503212 2208 78-79 0.07365925095818326 2208 80-81 0.1586696130924672 2208 82-83 0.145936369268032 2208 84-85 0.17584848535745934 2208 86-87 0.03412618621678121 2208 88-89 -0.12117771514903453 2208 90-91 -0.7562292052339252 2208 92-93 -2.1491826267983463 2208 94-95 -2.3304164488585037 2208 96-97 -2.2996591717417143 2208 98-99 -2.815113931406433 2208 100 -3.2377223603721887 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 271094.0 3 121017.0 4 121545.0 5 99740.0 6 91636.0 7 93701.0 8 99203.0 9 111539.0 10 128617.0 11 145718.0 12 163835.0 13 192850.0 14 243601.0 15 312126.0 16 369295.0 17 361804.0 18 340172.0 19 320211.0 20 323842.0 21 339520.0 22 354984.0 23 375137.0 24 398656.0 25 425257.0 26 459648.0 27 503908.0 28 563156.0 29 641185.0 30 738271.0 31 855606.0 32 1011817.0 33 1230846.0 34 1587022.0 35 2160870.0 36 2762428.0 37 2350292.0 38 700029.0 39 22756.0 40 12.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.162593986500976 28.02312808684164 27.815523479465565 21.99875444719181 2 23.669311889196567 27.76470164755501 27.106941833386745 21.459044629861683 3 23.60308109981208 26.454698520148963 26.895642961822446 23.04657741821651 4 24.437146145697838 25.96162570329123 26.6468559598695 22.954372191141424 5 24.79914067155744 26.186686093617734 26.26847057824786 22.74570265657697 6 24.598600392311234 26.03905844743646 25.823064297279974 23.539276862972333 7 24.504965039485977 26.307984886321357 25.978393978350223 23.20865609584244 8 23.987147354282683 26.197771752460593 26.308769494781934 23.50631139847479 9 24.014914339310334 26.02185264365382 26.325959135383414 23.637273881652433 10-11 23.940046604261976 26.134081878579433 26.3716726003967 23.554198916761887 12-13 23.653599615376613 26.16800955294543 26.55041719343195 23.627973638246 14-15 23.27502958559966 26.36582722533354 27.136265877542826 23.222877311523977 16-17 23.084109261739243 26.316594496411934 27.315691916345582 23.283604325503244 18-19 22.709775853500823 26.215056912987716 28.0573379518598 23.01782928165166 20-21 22.414710816049332 26.06436021755681 28.897553363537014 22.623375602856843 22-23 22.41151947373141 26.004569950194632 29.242445784166836 22.341464791907118 24-25 22.301621776387098 25.83238118610841 29.647671051978065 22.218325985526427 26-27 22.333133951498045 25.877805174341933 29.756752602793053 22.03230827136697 28-29 22.406875403229616 25.960620202087625 29.710371638288557 21.922132756394202 30-31 22.374855327607666 25.8875345529174 29.687762391995733 22.049847727479204 32-33 22.39002985876127 25.978300277533716 29.560881844081884 22.070788019623127 34-35 22.47942015681356 26.009748241595343 29.597189871475127 21.91364173011597 36-37 22.50410966613763 25.990810858035463 29.457874654142735 22.047204821684172 38-39 22.528605698361037 26.10448218383962 29.2786918135697 22.08822030422964 40-41 22.630479445835483 26.27216651879307 28.9855302800952 22.111823755276244 42-43 22.790380343462868 26.36280056927506 28.516817463352893 22.33000162390918 44-45 22.84082434487052 26.6019342774955 28.07921209356758 22.4780292840664 46-47 23.08423125242722 26.77479605751394 27.623962758304245 22.517009931754597 48-49 23.146347791672415 26.827424712315 27.281172196465555 22.745055299547033 50-51 23.192162142699928 26.93585648301854 27.045650506802865 22.826330867478664 52-53 23.347218588112078 27.00228431820867 26.85662645688538 22.793870636793873 54-55 23.38391967949957 26.96223892202959 26.797945289523877 22.85589610894696 56-57 23.385782395318557 27.04089420681316 26.745878020352198 22.82744537751608 58-59 23.447506663512932 27.132111447779657 26.662498562588404 22.757883326119007 60-61 23.434738848836567 27.10339934188794 26.609087217600248 22.852774591675246 62-63 23.43244825314301 27.22421660628353 26.520274139534454 22.823061001039 64-65 23.491685910615377 27.340660303337412 26.41317828973588 22.75447549631133 66-67 23.494350529031212 27.380044427617523 26.265142065485463 22.860462977865804 68-69 23.49991035282422 27.48875273280142 26.155172985625857 22.856163928748504 70-71 23.59563196812703 27.58204352876263 26.07520800747253 22.747116495637805 72-73 23.565559554487372 27.658077585676548 25.868599748271116 22.907763111564964 74-75 23.637322305113802 27.750544191246394 25.79556735063831 22.816566153001492 76-77 23.144403621078148 28.17802379740087 25.550780007632547 23.12679257388844 78-79 23.40613163075239 28.101047258196388 25.486908232360207 23.005912878691014 80-81 23.35687166683902 28.23344314606078 25.340186016539416 23.06949917056079 82-83 23.34204546511709 28.385339421353823 25.18975977638301 23.082855337146082 84-85 23.30073029993845 28.356497781275408 25.084012530406763 23.25875938837938 86-87 23.20082093356276 28.549286680501602 24.917592496672473 23.332299889263165 88-89 23.379211147172843 28.58574778904402 24.7375250849253 23.297515978857845 90-91 23.159070365338827 28.675390391048396 24.711208495844105 23.454330747768672 92-93 23.12339503158926 28.84902013808075 24.583014978809217 23.444569851520775 94-95 23.17647474580231 28.843515622830733 24.415706887829728 23.564302743537226 96-97 23.082834873415653 28.932307761782088 24.16571686859605 23.819140496206206 98-99 23.05534475221693 28.97514658437939 24.073796586787957 23.895712076615723 100 23.87113934334654 28.935552375819274 23.454552338890288 23.738755941943896 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1414.0 1 1061.0 2 655.5 3 594.5 4 677.5 5 842.0 6 1015.0 7 1222.0 8 1495.0 9 1871.0 10 2331.5 11 2964.5 12 3790.5 13 5066.5 14 7531.5 15 10496.0 16 15054.0 17 18232.0 18 19298.0 19 22956.0 20 34339.0 21 42299.0 22 45791.5 23 55688.5 24 67771.0 25 82177.5 26 99379.5 27 120937.5 28 145734.0 29 171379.5 30 197996.0 31 228198.0 32 264256.0 33 303685.0 34 346936.0 35 398034.5 36 456212.5 37 523780.0 38 590122.5 39 644685.5 40 692587.0 41 747956.5 42 825488.5 43 898381.5 44 942464.5 45 969303.0 46 964710.5 47 925675.0 48 885786.0 49 863510.5 50 834238.0 51 776883.0 52 701941.0 53 624291.5 54 558679.5 55 505728.0 56 452978.5 57 403511.0 58 365326.5 59 331694.5 60 296778.5 61 261922.0 62 232123.5 63 206775.5 64 183582.5 65 160141.5 66 138611.0 67 119307.5 68 101182.5 69 85493.5 70 71859.0 71 60142.0 72 50491.0 73 42393.0 74 35324.5 75 28950.0 76 23525.0 77 19180.5 78 15640.0 79 12545.5 80 9842.0 81 7606.5 82 5821.5 83 4332.0 84 3149.5 85 2252.5 86 1629.5 87 1209.5 88 847.0 89 606.5 90 448.5 91 303.5 92 192.5 93 138.0 94 99.0 95 52.0 96 26.5 97 13.5 98 5.5 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06021143604999517 2 0.007250053358709923 3 0.0021268692960754445 4 6.403980078293097E-4 5 0.006347886822132866 6 0.0011078418091645722 7 0.0031271990309329066 8 0.0028934771302652753 9 5.188626194821414E-4 10-11 0.0032557460763001037 12-13 0.0027625928658914015 14-15 0.0036554105264417535 16-17 0.004384622856524763 18-19 0.0025849642213840018 20-21 0.0034614213488876194 22-23 0.0017365537219605003 24-25 0.00237695172978981 26-27 7.385612061097148E-4 28-29 2.2437302464092606E-4 30-31 0.0010073413918774909 32-33 0.001075120743071104 34-35 0.002208671961309116 36-37 4.160249831883837E-4 38-39 4.3472273524179417E-4 40-41 0.0011101790281712485 42-43 5.796303136557256E-4 44-45 7.105145780295991E-4 46-47 7.011657020028939E-4 48-49 0.0014981573832795166 50-51 7.502473011430964E-4 52-53 0.0016290416476533902 54-55 7.058401400162464E-4 56-57 0.0010353880199576067 58-59 0.001523866792352956 60-61 0.0046580774803058915 62-63 0.005665418872183382 64-65 0.005405987562442312 66-67 0.0060627461033183555 68-69 0.0035034912910077926 70-71 0.00713786684638946 72-73 0.007843706986405706 74-75 0.011630001777221333 76-77 0.01385035983356383 78-79 0.011774909355635264 80-81 0.0121067944545833 82-83 0.009746203257840223 84-85 0.00907074696491077 86-87 0.007899800242565936 88-89 0.0028794538162252173 90-91 6.240374747825755E-4 92-93 0.0018277052632208767 94-95 0.0016080066765933032 96-97 0.007278099986790038 98-99 0.0026854646386710833 100 0.0025054987751570077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.1392946E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.5282266505045 #Duplication Level Percentage of deduplicated Percentage of total 1 79.75210461260082 34.714676854320345 2 10.931994455829804 9.517006648308366 3 3.5762399427135154 4.670021483490634 4 1.588383639085516 2.765580922002699 5 0.8995469343158415 1.957784141983322 6 0.5496456967660135 1.4355061479783302 7 0.373310984667224 1.1374695606202556 8 0.2958351604604329 1.0301743932568073 9 0.23034257994467258 0.9023763624384294 >10 1.4623213093396994 12.009582539056893 >50 0.1154660645674092 3.553201813788167 >100 0.2104177548895027 21.429619674307872 >500 0.013571324470648048 3.6721915817317 >1k 6.795074664678194E-4 0.49923915712586425 >5k 6.463056108505444E-5 0.21455279430446073 >10k+ 7.540232126589683E-5 0.491015925285774 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 4.674438013352626E-6 3 0.0 4.674438013352626E-6 0.0 0.0 9.348876026705252E-6 4 0.0 4.674438013352626E-6 0.0 0.0 2.8046628080115754E-5 5 4.674438013352626E-6 4.674438013352626E-6 0.0 0.0 2.8046628080115754E-5 6 4.674438013352626E-6 9.348876026705252E-6 0.0 0.0 4.206994212017363E-5 7 4.674438013352626E-6 9.348876026705252E-6 0.0 0.0 4.674438013352626E-5 8 1.4023314040057877E-5 9.348876026705252E-6 0.0 0.0 5.141881814687888E-5 9 1.8697752053410504E-5 9.348876026705252E-6 0.0 0.0 5.609325616023151E-5 10-11 9.115154126037621E-5 1.8697752053410504E-5 0.0 4.674438013352626E-6 5.609325616023151E-5 12-13 3.692806030548574E-4 1.8697752053410504E-5 0.0 4.674438013352626E-6 5.609325616023151E-5 14-15 8.928176605503516E-4 2.337219006676313E-5 0.0 4.674438013352626E-6 5.843047516690782E-5 16-17 0.002098822667995329 2.5709409073439443E-5 0.0 4.674438013352626E-6 6.310491318026045E-5 18-19 0.00385407414200924 3.0383847086792065E-5 0.0 4.674438013352626E-6 6.777935119361308E-5 20-21 0.006030025037224888 3.739550410682101E-5 0.0 4.674438013352626E-6 7.011657020028939E-5 22-23 0.008664070857749092 3.739550410682101E-5 0.0 4.674438013352626E-6 7.24537892069657E-5 24-25 0.012090433921536567 4.206994212017363E-5 0.0 4.674438013352626E-6 7.479100821364202E-5 26-27 0.01597956634864595 4.206994212017363E-5 0.0 7.011657020028939E-6 7.479100821364202E-5 28-29 0.020146827837549817 4.674438013352626E-5 0.0 9.348876026705252E-6 7.946544622699463E-5 30-31 0.023608249186437436 4.674438013352626E-5 0.0 1.8697752053410504E-5 7.946544622699463E-5 32-33 0.02631474879616861 5.141881814687888E-5 0.0 1.8697752053410504E-5 7.946544622699463E-5 34-35 0.029086690538086712 5.141881814687888E-5 2.337219006676313E-6 1.8697752053410504E-5 9.115154126037621E-5 36-37 0.03189836500311832 5.3756037153555194E-5 4.674438013352626E-6 1.8697752053410504E-5 1.098492933137867E-4 38-39 0.03455344579470261 6.076769417358414E-5 4.674438013352626E-6 1.8697752053410504E-5 1.1686095033381565E-4 40-41 0.03692104864846571 6.076769417358414E-5 4.674438013352626E-6 2.337219006676313E-5 1.285470453671972E-4 42-43 0.03945225683269616 6.310491318026045E-5 4.674438013352626E-6 2.337219006676313E-5 1.3789592139390244E-4 44-45 0.04242519940918843 7.011657020028939E-5 7.011657020028939E-6 2.337219006676313E-5 1.449075784139314E-4 46-47 0.04591233016714949 7.479100821364202E-5 9.348876026705252E-6 2.337219006676313E-5 1.5425645444063665E-4 48-49 0.04966356667286497 7.479100821364202E-5 9.348876026705252E-6 2.337219006676313E-5 1.636053304673419E-4 50-51 0.05400144514925621 7.712822722031832E-5 9.348876026705252E-6 2.337219006676313E-5 1.6827976848069452E-4 52-53 0.05903114045162364 7.946544622699463E-5 9.348876026705252E-6 2.5709409073439443E-5 1.7295420649404716E-4 54-55 0.06485315299725433 7.946544622699463E-5 9.348876026705252E-6 3.272106609346838E-5 1.7762864450739978E-4 56-57 0.07190921717841012 8.413988424034726E-5 9.348876026705252E-6 3.739550410682101E-5 1.7762864450739978E-4 58-59 0.08058029969317923 8.881432225369989E-5 9.348876026705252E-6 3.739550410682101E-5 1.7762864450739978E-4 60-61 0.09061398088884065 9.115154126037621E-5 9.348876026705252E-6 3.739550410682101E-5 1.7762864450739978E-4 62-63 0.10287970623587794 1.0050041728708145E-4 9.348876026705252E-6 3.739550410682101E-5 1.7762864450739978E-4 64-65 0.11756912769283856 1.0283763629375776E-4 9.348876026705252E-6 3.739550410682101E-5 1.7762864450739978E-4 66-67 0.13539509705675878 1.0751207430711039E-4 9.348876026705252E-6 4.4407161126849944E-5 1.7996586351407608E-4 68-69 0.1565889990092996 1.0751207430711039E-4 1.4023314040057877E-5 4.9081599140202566E-5 1.8931473954078134E-4 70-71 0.18176318493021018 1.0751207430711039E-4 1.636053304673419E-5 5.141881814687888E-5 1.963263965608103E-4 72-73 0.21200913609560834 1.0751207430711039E-4 1.8697752053410504E-5 5.3756037153555194E-5 2.010008345741629E-4 74-75 0.24852350863691236 1.098492933137867E-4 2.337219006676313E-5 6.076769417358414E-5 2.010008345741629E-4 76-77 0.29185321180168455 1.1452373132713934E-4 2.337219006676313E-5 6.544213218693676E-5 2.033380535808392E-4 78-79 0.3420309666560183 1.1686095033381565E-4 2.8046628080115754E-5 6.777935119361308E-5 2.1034971060086816E-4 80-81 0.4002347315792785 1.285470453671972E-4 2.8046628080115754E-5 7.011657020028939E-5 2.1034971060086816E-4 82-83 0.4671913816825415 1.3322148338054982E-4 2.8046628080115754E-5 7.011657020028939E-5 2.2437302464092603E-4 84-85 0.5425503341148059 1.4023314040057877E-4 2.8046628080115754E-5 7.011657020028939E-5 2.2437302464092603E-4 86-87 0.6297613241299258 1.4023314040057877E-4 3.0383847086792065E-5 7.479100821364202E-5 2.2671024364760234E-4 88 0.7011843997549473 1.4023314040057877E-4 3.272106609346838E-5 7.479100821364202E-5 2.2904746265427865E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCTCG 10370 0.0 38.572803 2 ATCTCGA 13780 0.0 32.287872 1 ATATCTC 13455 0.0 30.131521 1 GATCCGG 23990 0.0 27.094133 1 TCTCGAG 17225 0.0 25.841703 2 ATCCGGC 30100 0.0 21.690329 2 CTCGAGG 21320 0.0 20.832848 3 GGATCCG 16160 0.0 19.80673 5 ATGGATC 16985 0.0 19.757713 3 TATGGAT 17820 0.0 18.991322 2 TCCGGCG 37585 0.0 17.482222 3 TGGATCC 19140 0.0 17.16498 4 ATATGGA 20010 0.0 17.087748 1 TCGAGGG 25910 0.0 17.069872 4 GCGCCGT 38815 0.0 16.722147 9 CGTCGAC 37255 0.0 15.618199 12-13 CGGCGCG 42575 0.0 15.190456 5 AGGGCGC 42370 0.0 14.97634 2 GCCGTCG 39260 0.0 14.91047 10-11 TCGACTT 39185 0.0 14.903028 14-15 >>END_MODULE