FastQCFastQC Report
Wed 25 May 2016
SRR491009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44798733
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA281050.044.5983431
TCTCGAG338700.036.844092
CTCGAGG437500.028.4909343
GATCCGG443750.026.4764181
TCGAGGG515300.024.052694
ATCCGGC550700.021.3631022
TATCTCG190400.018.7613962
TCCGGCG686750.017.2127633
GCGCCGT694050.016.8417269
GGATCCG298300.016.6233182
CGAGGGC755200.016.4804735
ATATCTC226250.016.4434531
ATGGATC311450.016.1886861
CGGCGCG757650.015.4035845
AGGGCGC800500.015.3590857
CGTCGAC661300.014.388912-13
TGGATCC362700.014.1346181
CCGGCGC846150.013.9646994
TCGACTT691250.013.80286414-15
GCCGTCG705900.013.6162810-11
Produced by FastQC (version 0.11.5)