FastQCFastQC Report
Wed 25 May 2016
SRR491008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36989021
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT5733621.5500869839188227No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4334361.171796355464504No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT3927821.0618880667320176No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT2601380.7032843610540543No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1729910.4676820184021632No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1117830.3022058896881861No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080830.29220292151014216No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1057460.28588483052849656No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT945990.25574886126345436No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT892480.2412824064740724No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT846880.22895442407086147No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT762300.20608817951683553No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT754010.2038469739439711No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT726570.19642855646274066No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT654560.1769606175843367No Hit
TCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT649000.17545746885271712No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT624060.16871492759973292No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT623530.16857164183934473No Hit
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT586610.15859030170060462No Hit
GGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT559350.1512205473078079No Hit
GCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488760.1321365061270478No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438670.11859465001790666No Hit
GGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388960.10515552709545894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC882850.081.93761
TATCTCG937550.076.583042
ATATGGA776950.066.314691
TATGGAT826050.062.018342
ATGGATC925650.055.1778373
ATCTCGA1357550.052.7618643
TGGATCC1024650.049.5530934
TCTCGAG1485400.047.873134
GGATCCG1083900.046.4928785
CTCGAGG1706350.041.4454155
TCGAGGG1824450.038.5917176
GATCCGG1443350.034.786166
CGAGGGC2109300.033.3190167
ATCCGGC1651600.030.026217
GAGGGCG2382000.029.4547868
AGGGCGC2422800.028.8604379
TCCGGCG1858250.026.651318
CCGGCGC2124450.023.0635649
GGGCGCG2599200.015.3183428
CGGCGCG2210650.014.3731885
Produced by FastQC (version 0.11.5)