FastQCFastQC Report
Wed 25 May 2016
SRR491007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26736090
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT3689061.379805349248899No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT2706581.0123320201271016No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1313610.49132464769530626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1151870.4308296388888577No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT787670.2946092715875807No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT537340.20097927557844097No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT497380.18603318585477532No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464140.1736005526612156No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458280.17140875872276015No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432060.16160178994011468No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394300.1474785580090432No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT389190.14556728377260847No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT388320.1452418809182644No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362610.13562566553299304No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338020.126428359569406No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT334360.12505942342354473No Hit
TCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318890.11927323703653002No Hit
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301510.1127726604750358No Hit
GCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294610.1101918792164449No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290760.10875187807940503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC615600.080.774921
TATCTCG657750.075.22192
ATATGGA579000.064.953171
TATGGAT597900.062.588842
ATGGATC636800.058.4777573
ATCTCGA884150.055.6306653
TGGATCC690250.053.7093544
GGATCCG725050.050.787715
TCTCGAG964900.050.616144
CTCGAGG1072500.045.3669935
TCGAGGG1147050.042.2296036
GATCCGG929250.039.5535436
CGAGGGC1336350.036.2186977
ATCCGGC1064450.034.2506987
GAGGGCG1509300.032.046438
AGGGCGC1515950.031.8212179
TCCGGCG1201900.030.243628
CCGGCGC1371600.026.2585039
GGCGCGC3151350.015.2185910-11
GGGCGCG1649450.014.73953410-11
Produced by FastQC (version 0.11.5)