##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491007_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26736090
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.801605919190127	33.0	31.0	34.0	30.0	34.0
2	32.0920344747493	34.0	31.0	34.0	30.0	34.0
3	32.169532568150395	34.0	31.0	34.0	30.0	34.0
4	35.53086453553979	37.0	35.0	37.0	33.0	37.0
5	35.362396969788776	37.0	35.0	37.0	33.0	37.0
6	35.277866471873786	37.0	35.0	37.0	33.0	37.0
7	35.23824209149505	37.0	35.0	37.0	33.0	37.0
8	35.19781804295243	37.0	35.0	37.0	33.0	37.0
9	36.88801361754842	39.0	38.0	39.0	33.0	39.0
10-11	36.81725052167314	39.0	37.0	39.0	33.0	39.0
12-13	36.758678045293834	39.0	37.0	39.0	33.0	39.0
14-15	38.11283510416071	41.0	38.0	41.0	33.0	41.0
16-17	38.06703880410337	41.0	38.0	41.0	33.0	41.0
18-19	37.98551813298055	41.0	38.0	41.0	33.0	41.0
20-21	37.92344146058754	40.5	38.0	41.0	33.0	41.0
22-23	37.83005815360436	40.0	38.0	41.0	33.0	41.0
24-25	37.730376805284536	40.0	38.0	41.0	33.0	41.0
26-27	37.43254473634701	40.0	37.5	41.0	32.5	41.0
28-29	37.151811259612	40.0	36.5	41.0	32.0	41.0
30-31	36.85211420218887	40.0	35.5	41.0	31.5	41.0
32-33	36.4628117649215	40.0	35.0	41.0	30.5	41.0
34-35	36.181971597193154	40.0	35.0	41.0	30.0	41.0
36-37	35.84095466090966	39.0	35.0	41.0	29.5	41.0
38-39	35.50743085469865	39.0	35.0	41.0	27.5	41.0
40-41	35.219391541545534	39.0	35.0	41.0	25.5	41.0
42-43	35.05330065091792	39.5	35.0	41.0	23.5	41.0
44-45	34.79260086272899	40.0	35.0	41.0	20.5	41.0
46-47	34.434119536551535	39.5	35.0	41.0	15.5	41.0
48-49	33.962883091731065	39.0	34.5	41.0	7.5	41.0
50-51	33.60206488682526	39.0	34.0	41.0	3.5	41.0
52-53	33.321442533294885	39.0	34.0	41.0	2.0	41.0
54-55	33.0021003445156	38.0	33.0	41.0	2.0	41.0
56-57	32.70028238983337	38.0	33.0	41.0	2.0	41.0
58-59	32.3596384699483	37.0	33.0	40.0	2.0	41.0
60-61	32.02340388964878	37.0	32.0	40.0	2.0	41.0
62-63	31.653048762926815	36.0	32.0	40.0	2.0	41.0
64-65	31.245345373986996	35.5	31.0	39.5	2.0	41.0
66-67	30.837178660006003	35.0	31.0	39.0	2.0	41.0
68-69	30.413545436150162	35.0	30.5	39.0	2.0	41.0
70-71	29.95342776374556	35.0	30.0	38.0	2.0	40.0
72-73	29.42898166859851	35.0	29.0	37.0	2.0	40.0
74-75	28.99431667083706	35.0	29.0	37.0	2.0	39.0
76-77	28.23491638081709	34.0	27.5	36.0	2.0	39.0
78-79	28.2378208631105	35.0	28.0	36.0	2.0	38.5
80-81	27.867217663465375	34.5	27.0	35.0	2.0	37.0
82-83	27.443074641804394	34.0	26.0	35.0	2.0	37.0
84-85	26.995918008205386	34.0	25.0	35.0	2.0	36.0
86-87	26.58087859892752	34.0	24.0	35.0	2.0	36.0
88-89	26.14790734172424	34.0	21.5	35.0	2.0	36.0
90-91	25.69378162625874	34.0	18.5	35.0	2.0	35.0
92-93	25.231606416645064	34.0	8.0	35.0	2.0	35.0
94-95	24.786084315245798	33.0	2.0	35.0	2.0	35.0
96-97	24.29971689951672	33.0	2.0	35.0	2.0	35.0
98-99	23.811082061737523	33.0	2.0	35.0	2.0	35.0
100	22.910810294250208	32.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18366365719353084
1101	2	0.17307213098299457
1101	3	0.15875198312830463
1101	4	0.1407798598277239
1101	5	0.1706954323136074
1101	6	0.1981737363961642
1101	7	0.1938351079656755
1101	8	0.1798144125097778
1101	9	0.19434522892747452
1101	10-11	0.23095935844171578
1101	12-13	0.21552499016506488
1101	14-15	0.24597149725953926
1101	16-17	0.25108420741126025
1101	18-19	0.2745305899527608
1101	20-21	0.2577785647330728
1101	22-23	0.24315426245012617
1101	24-25	0.23082931791830674
1101	26-27	0.23772269038319394
1101	28-29	0.21496295573572155
1101	30-31	0.2306405518374106
1101	32-33	0.2511085455872575
1101	34-35	0.21104525505872118
1101	36-37	0.18553026056635957
1101	38-39	0.20675845426981
1101	40-41	0.15734975557700892
1101	42-43	0.16958777499657174
1101	44-45	0.13571252675306766
1101	46-47	0.1305344264484205
1101	48-49	0.1738567214128821
1101	50-51	0.149444110993727
1101	52-53	0.09521081311066126
1101	54-55	0.07342268124861562
1101	56-57	0.10225549360330888
1101	58-59	0.10860896699721678
1101	60-61	0.04998487774902571
1101	62-63	0.029518663867833084
1101	64-65	0.01527380332073136
1101	66-67	-0.04474854985891952
1101	68-69	-0.00459941527986274
1101	70-71	-0.01570545998896833
1101	72-73	0.0388105271497281
1101	74-75	-0.05378214933525172
1101	76-77	-0.10247583414104611
1101	78-79	-0.06264597733438038
1101	80-81	0.01995220209009574
1101	82-83	-4.525136910054073E-4
1101	84-85	-0.02528004728933908
1101	86-87	-0.056496265127819356
1101	88-89	-0.13298170514051932
1101	90-91	-0.11557513464061486
1101	92-93	-0.1318450795212769
1101	94-95	-0.17686225889628204
1101	96-97	-0.16430377538851104
1101	98-99	-0.20231145988014987
1101	100	-0.1445720149617813
1102	1	0.14066831396802115
1102	2	0.15433953295770664
1102	3	0.16026076678847545
1102	4	0.13575151986195522
1102	5	0.16050327378172824
1102	6	0.15442834318256615
1102	7	0.15634653175081326
1102	8	0.13463887440785527
1102	9	0.18490192646785886
1102	10-11	0.18610066989655394
1102	12-13	0.1619445634186576
1102	14-15	0.2079459970728763
1102	16-17	0.23997699025601094
1102	18-19	0.26657307285074694
1102	20-21	0.25729473471597686
1102	22-23	0.28945829398010403
1102	24-25	0.24879326227927834
1102	26-27	0.2270870251123398
1102	28-29	0.1921736240681966
1102	30-31	0.23325198356764076
1102	32-33	0.2806703822599701
1102	34-35	0.2832082471025501
1102	36-37	0.27255453271475716
1102	38-39	0.2603376882550492
1102	40-41	0.22242914292319682
1102	42-43	0.23494994224625998
1102	44-45	0.18702104698427036
1102	46-47	0.1983952350225593
1102	48-49	0.23316819088866936
1102	50-51	0.233909635463192
1102	52-53	0.21675755129884777
1102	54-55	0.15855609308466967
1102	56-57	0.19067428434355804
1102	58-59	0.21638655900490278
1102	60-61	0.19284348171915866
1102	62-63	0.18953295394828729
1102	64-65	0.265100801651581
1102	66-67	0.21071521341876576
1102	68-69	0.20881098373977736
1102	70-71	0.21965183491629503
1102	72-73	0.20533380185354133
1102	74-75	0.15549059715169378
1102	76-77	0.16263821846370874
1102	78-79	0.18394144191078965
1102	80-81	0.21699150277189005
1102	82-83	0.17663734956023092
1102	84-85	0.09806425967107302
1102	86-87	0.09641475165467739
1102	88-89	0.07263293818568073
1102	90-91	0.06781839600122197
1102	92-93	0.011004475068354225
1102	94-95	-0.05757623413872537
1102	96-97	-0.07465180980889485
1102	98-99	-0.08102848108789118
1102	100	-0.0367822614418003
1103	1	0.038391438665566824
1103	2	0.01247663905938623
1103	3	0.01319160698923838
1103	4	0.017532782543398184
1103	5	0.016080720267709125
1103	6	0.05968205781316982
1103	7	0.08620028092023091
1103	8	0.057045290005966365
1103	9	0.07073903025220574
1103	10-11	0.09535985516637169
1103	12-13	0.10088190966521893
1103	14-15	0.13562559554992504
1103	16-17	0.08357668597844992
1103	18-19	0.10863737571446563
1103	20-21	0.09023102116489667
1103	22-23	0.08642194682158078
1103	24-25	0.07493382467559684
1103	26-27	0.07512504536681774
1103	28-29	0.10222830905345148
1103	30-31	0.12088325639723507
1103	32-33	0.07245295486112013
1103	34-35	0.06646839107281721
1103	36-37	0.04237773482332585
1103	38-39	0.11945733938496517
1103	40-41	0.10911854237840402
1103	42-43	0.10304341397051786
1103	44-45	0.09883911004342849
1103	46-47	0.1224680781711669
1103	48-49	0.06887968014513746
1103	50-51	0.10041611277498674
1103	52-53	0.13005601696915647
1103	54-55	0.12396767655273067
1103	56-57	0.07041764179697907
1103	58-59	0.12375151649997207
1103	60-61	0.10360129202464918
1103	62-63	0.13557300822670015
1103	64-65	0.16078842093593693
1103	66-67	0.12349418809223422
1103	68-69	0.12384323104105732
1103	70-71	0.11443987296319591
1103	72-73	-0.122490397394305
1103	74-75	0.08528765591886867
1103	76-77	0.09286839906042488
1103	78-79	0.06814947531930571
1103	80-81	0.13849925608450064
1103	82-83	0.15861785752266755
1103	84-85	0.06110052553649936
1103	86-87	0.011983360610511085
1103	88-89	-0.020950256627024544
1103	90-91	0.04784633895427248
1103	92-93	-0.043479980961329545
1103	94-95	-0.0443824121352101
1103	96-97	0.013130961933857321
1103	98-99	-0.05509553518214716
1103	100	0.02798910001245858
1104	1	0.058629118092412114
1104	2	0.04640470954088016
1104	3	0.028851112840769133
1104	4	0.028661116840766
1104	5	0.0510892990872307
1104	6	0.0630961736203659
1104	7	0.0517862777795699
1104	8	0.029387446588991395
1104	9	0.028449551564840192
1104	10-11	0.06440189450452749
1104	12-13	0.0964136403491409
1104	14-15	0.09059943494722944
1104	16-17	0.0761931068304662
1104	18-19	0.08145320206121909
1104	20-21	0.06737277465828839
1104	22-23	0.09340609699864189
1104	24-25	0.09702499366042616
1104	26-27	0.069422522129031
1104	28-29	0.06499161287087674
1104	30-31	0.0583322714923753
1104	32-33	0.09226062683642766
1104	34-35	0.09689748170735868
1104	36-37	0.08692324270183605
1104	38-39	0.040521822308669186
1104	40-41	0.011514991303023692
1104	42-43	0.022631764058999693
1104	44-45	-0.09041026929458695
1104	46-47	-9.375188764124687E-4
1104	48-49	0.036040772855066905
1104	50-51	0.02498663114877786
1104	52-53	0.0017071736446112595
1104	54-55	0.05142865985874323
1104	56-57	0.03465480688417699
1104	58-59	0.04575643864021828
1104	60-61	0.008394918153562969
1104	62-63	-0.017634555419874687
1104	64-65	0.06573316491170189
1104	66-67	0.029505220606424132
1104	68-69	-0.01702534731910532
1104	70-71	-0.012350633838252634
1104	72-73	0.0021756653425839545
1104	74-75	-0.0702819370060368
1104	76-77	-0.06079573367603075
1104	78-79	-0.09593406426697015
1104	80-81	-0.06170512217055801
1104	82-83	-0.04687731763561587
1104	84-85	-0.07956146823329036
1104	86-87	-0.032171418737441115
1104	88-89	-0.020887834719960807
1104	90-91	0.0029801371059612336
1104	92-93	-0.0038001410817543047
1104	94-95	-0.029344144411084017
1104	96-97	4.456012755476024E-4
1104	98-99	0.04328881138729912
1104	100	0.11406159480941724
1105	1	6.17775358051631E-4
1105	2	-0.040057913891665464
1105	3	-0.05370243335118374
1105	4	-0.026471905430476284
1105	5	0.005019592350855362
1105	6	-0.0060246715475358315
1105	7	-0.023131998729930103
1105	8	-0.028658340016384898
1105	9	-0.04110582103190552
1105	10-11	-0.015930772079926214
1105	12-13	-0.03278884067213994
1105	14-15	-0.021771896952110126
1105	16-17	-0.026483825257820115
1105	18-19	-0.08208113272304018
1105	20-21	-0.03863735851209782
1105	22-23	-0.04563753374785762
1105	24-25	-0.013657313161644424
1105	26-27	-0.014664489990124707
1105	28-29	-0.03373699519793405
1105	30-31	-0.022968168189891003
1105	32-33	0.014541289480391129
1105	34-35	-0.019199635154755867
1105	36-37	-0.046737035262403026
1105	38-39	0.019531995551453463
1105	40-41	-0.035016772215364256
1105	42-43	-0.015981925976035427
1105	44-45	-0.011646600685807584
1105	46-47	-0.0030328759213773537
1105	48-49	0.04107644182533221
1105	50-51	-0.032159574671744906
1105	52-53	0.05814121569286357
1105	54-55	0.06316074106546665
1105	56-57	0.06386042091374122
1105	58-59	0.06724344359226819
1105	60-61	0.038218432487738596
1105	62-63	0.08681200824618429
1105	64-65	0.15256739241911887
1105	66-67	0.05668318168667952
1105	68-69	0.055004334979777525
1105	70-71	0.09763259322482298
1105	72-73	0.12850659816217913
1105	74-75	0.08382887488593127
1105	76-77	0.09388463532739166
1105	78-79	0.11632495408357713
1105	80-81	0.12904356832979857
1105	82-83	0.04200512048730687
1105	84-85	-0.04990425937137388
1105	86-87	0.07120818052496958
1105	88-89	0.1023137136421326
1105	90-91	0.14672090554194028
1105	92-93	0.09162512593458416
1105	94-95	0.04782473993089198
1105	96-97	0.04873756886753711
1105	98-99	0.06746636499059377
1105	100	0.10615966476429506
1106	1	-0.1857395172375078
1106	2	-0.20676358591414612
1106	3	-0.16246251591500993
1106	4	-0.17775272367607897
1106	5	-0.164415635846332
1106	6	-0.203593734047665
1106	7	-0.21815456946245604
1106	8	-0.2723179301830285
1106	9	-0.2706307081742523
1106	10-11	-0.24933036838964995
1106	12-13	-0.24464067160764102
1106	14-15	-0.29260683812100297
1106	16-17	-0.29561347294252016
1106	18-19	-0.27291664294301654
1106	20-21	-0.31068909881495443
1106	22-23	-0.260079829753181
1106	24-25	-0.3050429192875157
1106	26-27	-0.32366513829496313
1106	28-29	-0.3130820696061605
1106	30-31	-0.32694098014423645
1106	32-33	-0.2933383399584173
1106	34-35	-0.3202653622789171
1106	36-37	-0.3300365119272115
1106	38-39	-0.36158339569170295
1106	40-41	-0.3391264998000949
1106	42-43	-0.3626183725123724
1106	44-45	-0.3441502802510996
1106	46-47	-0.3226363146323621
1106	48-49	-0.2607380385187028
1106	50-51	-0.24780593220198455
1106	52-53	-0.2819599726885613
1106	54-55	-0.24711750054736825
1106	56-57	-0.2012037176957051
1106	58-59	-0.289152486098196
1106	60-61	-0.2754929751791515
1106	62-63	-0.259152870022465
1106	64-65	-0.29988732094009407
1106	66-67	-0.21685398558925328
1106	68-69	-0.3075141447819618
1106	70-71	-0.29363931527852927
1106	72-73	-0.27183980387141204
1106	74-75	-0.2756030103123699
1106	76-77	-0.2981166083356044
1106	78-79	-0.2411939581261251
1106	80-81	-0.21603698144851435
1106	82-83	-0.21443396754207456
1106	84-85	-0.22837295015061088
1106	86-87	-0.2140853156127136
1106	88-89	-0.26525365591040995
1106	90-91	-0.2002575588925275
1106	92-93	-0.2601086387255158
1106	94-95	-0.2603690640458467
1106	96-97	-0.17253196854085928
1106	98-99	-0.189792088432732
1106	100	-0.24764427108521048
1107	1	-0.3069157031968395
1107	2	-0.29114823041009785
1107	3	-0.29544035368071775
1107	4	-0.241578105886056
1107	5	-0.25269516576558004
1107	6	-0.3364170684069947
1107	7	-0.32628255005797513
1107	8	-0.30875617253631304
1107	9	-0.40170661884207703
1107	10-11	-0.36114459765006757
1107	12-13	-0.3939930132288154
1107	14-15	-0.40818780074524597
1107	16-17	-0.41433406631227143
1107	18-19	-0.4489895124446548
1107	20-21	-0.39210007838787675
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2207	82-83	0.13049957532869527
2207	84-85	0.1749009523331928
2207	86-87	0.17854184820896535
2207	88-89	0.21783885780743262
2207	90-91	0.2909566163528545
2207	92-93	0.33467980989624024
2207	94-95	0.33088294354019254
2207	96-97	0.2925676571871456
2207	98-99	0.26457177629167816
2207	100	0.26170934271227253
2208	1	-0.22845818810255736
2208	2	-0.18839629179608863
2208	3	-0.24664722579836962
2208	4	-0.19741044686826825
2208	5	-0.18516643387290088
2208	6	-0.2200747905485727
2208	7	-0.21948411471247908
2208	8	-0.2648078853671123
2208	9	-0.2654659650584108
2208	10-11	-0.23176915805746034
2208	12-13	-0.25678832514306293
2208	14-15	-0.35107003631770084
2208	16-17	-0.29985536286270786
2208	18-19	-0.32154828751114906
2208	20-21	-0.3307864215316698
2208	22-23	-0.325249383361367
2208	24-25	-0.32446389185180635
2208	26-27	-0.35896336244880445
2208	28-29	-0.3818455457801946
2208	30-31	-0.3893938407831925
2208	32-33	-0.3466605534255365
2208	34-35	-0.3934782137256505
2208	36-37	-0.376477720087145
2208	38-39	-0.36907749350068286
2208	40-41	-0.23207717512342896
2208	42-43	-0.29586564147663097
2208	44-45	-0.34179572138434366
2208	46-47	-0.34968164456243045
2208	48-49	-0.33019005530160683
2208	50-51	-0.5044242165306869
2208	52-53	-0.32834135688010946
2208	54-55	-0.2685847158173189
2208	56-57	-0.18844646963836453
2208	58-59	-0.5161243772068005
2208	60-61	-0.22538895959861094
2208	62-63	-0.5056630102723929
2208	64-65	-0.19041439634569812
2208	66-67	-0.10903408623627797
2208	68-69	-0.1037452962482881
2208	70-71	-0.04990730718570191
2208	72-73	-0.012806779338134788
2208	74-75	0.021420541272600957
2208	76-77	-0.026164882234493092
2208	78-79	0.0644422341157096
2208	80-81	0.13296329473400448
2208	82-83	0.18621649142427543
2208	84-85	0.239838456186245
2208	86-87	0.24355885270323796
2208	88-89	0.2936847202991153
2208	90-91	0.4293014589766422
2208	92-93	0.5581986706486823
2208	94-95	0.5438081010456237
2208	96-97	0.4570755672621587
2208	98-99	0.4597523957104741
2208	100	0.34264313708875704
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	342860.0
3	128348.0
4	70402.0
5	58526.0
6	61623.0
7	74884.0
8	89559.0
9	93075.0
10	92143.0
11	90360.0
12	101047.0
13	153586.0
14	269134.0
15	338412.0
16	377013.0
17	341061.0
18	338449.0
19	276563.0
20	218867.0
21	209914.0
22	221526.0
23	243637.0
24	272626.0
25	312189.0
26	355455.0
27	399817.0
28	456240.0
29	509374.0
30	578341.0
31	676677.0
32	788627.0
33	980560.0
34	1297514.0
35	1723452.0
36	2671088.0
37	4555197.0
38	5403635.0
39	1560250.0
40	4059.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.15061514015937	29.761263883130574	26.116190841424096	21.97193013528596
2	23.43010021144711	26.020851001306504	29.929626355658467	20.61942243158791
3	23.61891602693533	27.825397818905635	25.749855241817528	22.805830912341506
4	24.50876508186239	24.26239021954147	28.543214699173642	22.685629999422506
5	26.083932625576594	24.612100330561738	24.213702377118263	25.090264666743405
6	26.847606953045673	24.31819765887547	25.474632951391012	23.359562436687845
7	25.057418892925348	26.31488114119837	23.869498831864135	24.758201134012143
8	26.63949692247691	24.39543987350136	25.318775417820543	23.646287786201185
9	25.03315761810733	26.0314195802463	24.230158224552813	24.70526457709355
10-11	26.494803989228867	24.388081771508826	24.546068161959806	24.5710460773025
12-13	24.9371593747369	24.39985032600866	25.08266681634066	25.58032348291378
14-15	24.0821970569305	24.717332014830056	26.68911295386999	24.51135797436945
16-17	23.564538919327678	24.705650921180567	27.548982373098124	24.18082778639363
18-19	22.743673941657068	24.90619633581258	29.373638700184117	22.97649102234623
20-21	21.225397239099657	25.20177760836273	31.865713729460367	21.70711142307725
22-23	20.722974861381662	24.769640445332026	32.97796828229927	21.529416410987036
24-25	19.954343382022227	23.891576588578182	34.81093189136055	21.34314813803904
26-27	19.960624146382532	23.923852644577074	36.541201893393705	19.574321315646692
28-29	19.98149494954636	24.018349288572015	36.46892941550411	19.531226346377522
30-31	19.983504710481863	24.012197367658526	36.399334207959924	19.60496371389969
32-33	19.985992702914412	24.09583260273421	36.27309769013931	19.645077004212066
34-35	20.08283650369954	24.202374721022373	36.15553605713516	19.559252718142933
36-37	20.153636267047947	24.298899524237164	35.79780138333219	19.749662825382696
38-39	20.29211008257164	24.5327887309326	35.30476401827423	19.870337168221536
40-41	20.432977081450396	24.855214548091592	34.712885077954574	19.998923292503434
42-43	20.62897082358762	25.198414435544876	33.89216608227937	20.280448658588135
44-45	20.777194848044118	25.649248933734164	33.10306665252013	20.470489565701595
46-47	21.051901004454464	26.12757930604702	32.240964534298655	20.57955515519986
48-49	21.21264204543063	26.678392127781166	31.234054025428108	20.8749118013601
50-51	21.339436296368245	27.31068599835763	30.31434862924097	21.035529076033153
52-53	21.450680666933373	27.812176057663635	29.64580717687632	21.091336098526675
54-55	21.489419078032274	28.17667751481736	29.162932778566073	21.170970628584293
56-57	21.45693144181061	28.573456138266856	28.785516326028578	21.184096093893956
58-59	21.506537423402296	28.978089279664086	28.415182882620787	21.10019041431283
60-61	21.47966779092095	29.327815692873028	28.027383184845306	21.165133331360718
62-63	21.485395631211105	29.697623266958285	27.696351696179317	21.120629405651297
64-65	21.511797743499027	30.101325465290586	27.329383850000333	21.05749294121005
66-67	21.529126920439875	30.395489089781037	26.950856314793803	21.124527674985288
68-69	21.537936572761833	30.742429517689175	26.61463302692487	21.10500088262412
70-71	21.595221504613313	31.03819266921679	26.312138708863202	21.05444711730669
72-73	21.620079396839582	31.274294362327627	25.955493150913473	21.15013308991932
74-75	21.671647454557473	31.519684639087053	25.71622665020714	21.092441256148334
76-77	21.573807498787826	31.838374279183007	25.35269570242748	21.23512251960169
78-79	21.663880223720174	31.92270660570925	25.112202263494794	21.301210907075784
80-81	21.70642850792704	32.04584896657195	24.855591998223378	21.39213052727763
82-83	21.730424633497954	32.185595043351526	24.631130144042235	21.45285017910829
84-85	21.742081315102087	32.21409204776524	24.395766841593236	21.64805979553943
86-87	21.75689068334289	32.334518857205786	24.18427554605326	21.724314913398068
88-89	21.881456610845753	32.39930992588593	23.91685096159331	21.80238250167501
90-91	21.861509829491773	32.428705598785406	23.70767188179754	22.002112689925283
92-93	21.845078814569796	32.539406723796475	23.54782956026063	22.0676849013731
94-95	21.898319716621025	32.608309132269234	23.361561700113025	22.131809450996716
96-97	21.866871944030496	32.68848636371797	23.11891237409555	22.32572931815597
98-99	21.88866771616174	32.76841898829767	22.965823408707486	22.3770898868331
100	22.245929977794862	32.56115015613542	22.43637086561881	22.75654900045091
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18757.0
1	11358.0
2	3726.5
3	3460.5
4	3639.0
5	4040.5
6	4650.0
7	5500.5
8	6668.5
9	8883.5
10	12574.0
11	19618.0
12	27917.5
13	42858.5
14	72209.5
15	101554.0
16	274454.0
17	301205.0
18	150646.0
19	129324.5
20	123467.0
21	124968.5
22	132507.0
23	145807.5
24	162257.5
25	181143.5
26	204921.5
27	235004.0
28	269656.5
29	304776.0
30	338903.5
31	378353.5
32	420848.0
33	456547.0
34	487190.5
35	530223.5
36	583729.0
37	641136.5
38	700103.5
39	748522.5
40	792554.0
41	846108.0
42	925286.0
43	1004041.5
44	1063955.5
45	1096714.0
46	1078513.0
47	1030177.5
48	981955.0
49	960568.0
50	941647.5
51	892363.0
52	816066.5
53	730411.5
54	661655.5
55	606196.5
56	539429.0
57	473023.0
58	425389.5
59	381289.5
60	334076.5
61	288840.5
62	249901.5
63	217182.5
64	189532.5
65	162225.0
66	136468.0
67	113447.5
68	92379.0
69	74762.0
70	59756.5
71	47180.5
72	37676.0
73	30322.0
74	23707.0
75	18077.5
76	13694.5
77	10463.5
78	7975.0
79	5718.0
80	3920.0
81	2627.5
82	1727.5
83	1165.0
84	757.5
85	483.5
86	332.5
87	203.5
88	123.0
89	79.5
90	51.5
91	41.5
92	32.5
93	23.0
94	19.5
95	14.0
96	8.5
97	8.5
98	6.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06987932790471606
2	0.00829590265442703
3	0.026297786998772072
4	7.480525387220046E-6
5	0.02068739295835704
6	0.07942821856150244
7	0.047662167504672524
8	0.0898560709512872
9	0.07389262977495961
10-11	0.054175834985594376
12-13	0.04241457894553766
14-15	0.04431650252523836
16-17	0.04645593278598329
18-19	0.04608190651662229
20-21	0.03302838971592331
22-23	0.03777104281142082
24-25	0.034361793366195284
26-27	0.04334964461894017
28-29	0.05915973502482973
30-31	0.05885116335260691
32-33	0.042818527316447544
34-35	0.03631234036091291
36-37	0.04000023937681239
38-39	0.029340490700023824
40-41	0.024137785293212283
42-43	0.03348283163319692
44-45	0.023780590205972525
46-47	0.03271233751831326
48-49	0.027533943819010188
50-51	0.03311628588922314
52-53	0.02361414851610688
54-55	0.025445007104628988
56-57	0.030165218623964835
58-59	0.016386090860705513
60-61	0.012743074997129348
62-63	0.01715097458154876
64-65	0.014276582701509458
66-67	0.014345777561341243
68-69	0.022959602544725125
70-71	0.012744945128476154
72-73	0.01135543753780003
74-75	0.01135917780049364
76-77	0.007863902313315074
78-79	0.010411021207663499
80-81	0.016649779380605016
82-83	0.009507747767156679
84-85	0.004686549155093359
86-87	0.00559356285829379
88-89	0.0015372479670737193
90-91	0.0072018758165461
92-93	0.011196476373321603
94-95	0.003244677886706695
96-97	0.005722601921223335
98-99	0.004970809119807721
100	0.00480623756128888
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.673609E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.45947064149307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3930398479087	31.722667956589355
2	10.035763545822169	7.9201183400267245
3	3.2878890310983517	3.8921508208533746
4	1.572450589067206	2.4819227141798357
5	0.9379200409753019	1.850491416046645
6	0.6178644451087624	1.4628362359314968
7	0.4317162318092131	1.192470578217216
8	0.3247921617928791	1.0252901418282545
9	0.24913045852920554	0.8847500412811402
>10	1.727506857549895	13.47536333981326
>50	0.1967546574323772	5.418318126516194
>100	0.20980844741784155	17.50931389637513
>500	0.01327790830370009	3.3168236127261816
>1k	0.0016867223130389859	1.0544190298032161
>5k	1.0451437110666334E-4	0.2853339693608804
>10k+	2.945405003913794E-4	6.507729780451192
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	368906	1.379805349248899	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	270658	1.0123320201271016	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	131361	0.49132464769530626	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115187	0.4308296388888577	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78767	0.2946092715875807	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53734	0.20097927557844097	No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49738	0.18603318585477532	No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46414	0.1736005526612156	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45828	0.17140875872276015	No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43206	0.16160178994011468	No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39430	0.1474785580090432	No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38919	0.14556728377260847	No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38832	0.1452418809182644	No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36261	0.13562566553299304	No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33802	0.126428359569406	No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT	33436	0.12505942342354473	No Hit
TCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31889	0.11927323703653002	No Hit
AGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30151	0.1127726604750358	No Hit
GCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29461	0.1101918792164449	No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29076	0.10875187807940503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.480525387220046E-6	0.0	0.0	0.0	0.0
2	1.1220788080830069E-5	3.740262693610023E-6	0.0	0.0	0.0
3	1.1220788080830069E-5	3.740262693610023E-6	0.0	0.0	0.0
4	3.740262693610023E-5	3.740262693610023E-6	0.0	3.740262693610023E-6	0.0
5	8.976630464664055E-5	3.740262693610023E-6	0.0	3.740262693610023E-6	0.0
6	1.0098709272747063E-4	7.480525387220046E-6	0.0	3.740262693610023E-6	0.0
7	1.0472735542108064E-4	1.4961050774440092E-5	0.0	3.740262693610023E-6	0.0
8	1.0472735542108064E-4	1.8701313468050114E-5	0.0	7.480525387220046E-6	0.0
9	1.1968840619552074E-4	1.8701313468050114E-5	0.0	1.4961050774440092E-5	0.0
10-11	1.4400011370398587E-4	1.8701313468050114E-5	0.0	1.8701313468050114E-5	0.0
12-13	2.113248421889663E-4	1.8701313468050114E-5	0.0	1.8701313468050114E-5	0.0
14-15	3.403639051185121E-4	1.8701313468050114E-5	0.0	1.8701313468050114E-5	0.0
16-17	6.825979415838292E-4	1.8701313468050114E-5	0.0	1.8701313468050114E-5	0.0
18-19	0.0014082089041441736	2.0571444814855126E-5	0.0	2.2441576161660137E-5	0.0
20-21	0.002543378631654816	2.2441576161660137E-5	0.0	2.2441576161660137E-5	0.0
22-23	0.004465873656170368	2.2441576161660137E-5	0.0	2.431170750846515E-5	0.0
24-25	0.007056005571495308	2.2441576161660137E-5	0.0	2.618183885527016E-5	3.740262693610023E-6
26-27	0.010235228861063827	2.2441576161660137E-5	0.0	2.9922101548880184E-5	3.740262693610023E-6
28-29	0.018452585998925047	2.2441576161660137E-5	0.0	2.9922101548880184E-5	3.740262693610023E-6
30-31	0.024676383121092126	2.2441576161660137E-5	0.0	3.1792232895685196E-5	3.740262693610023E-6
32-33	0.02532344856708666	2.2441576161660137E-5	0.0	3.740262693610023E-5	3.740262693610023E-6
34-35	0.02602848808483215	2.2441576161660137E-5	0.0	3.740262693610023E-5	3.740262693610023E-6
36-37	0.026894358898402873	2.2441576161660137E-5	0.0	3.740262693610023E-5	3.740262693610023E-6
38-39	0.028033268888607122	2.2441576161660137E-5	0.0	3.740262693610023E-5	3.740262693610023E-6
40-41	0.029254464658070793	2.2441576161660137E-5	0.0	3.740262693610023E-5	5.610394040415034E-6
42-43	0.030677634612989408	2.2441576161660137E-5	0.0	3.740262693610023E-5	7.480525387220046E-6
44-45	0.03243368794763932	2.618183885527016E-5	0.0	4.114288962971025E-5	7.480525387220046E-6
46-47	0.034507663611246076	2.618183885527016E-5	0.0	4.114288962971025E-5	7.480525387220046E-6
48-49	0.03671441860047599	3.3662364242490205E-5	0.0	4.114288962971025E-5	7.480525387220046E-6
50-51	0.03956262864166002	3.3662364242490205E-5	0.0	4.114288962971025E-5	7.480525387220046E-6
52-53	0.04297748848092597	3.553249558929521E-5	0.0	4.114288962971025E-5	7.480525387220046E-6
54-55	0.04739660885342621	3.927275828290524E-5	3.740262693610023E-6	4.114288962971025E-5	7.480525387220046E-6
56-57	0.05261240517966539	4.114288962971025E-5	3.740262693610023E-6	4.114288962971025E-5	1.1220788080830069E-5
58-59	0.058858643877994124	4.114288962971025E-5	3.740262693610023E-6	4.114288962971025E-5	1.1220788080830069E-5
60-61	0.06631859782039931	4.4883152323320275E-5	3.740262693610023E-6	4.4883152323320275E-5	1.1220788080830069E-5
62-63	0.07559070903785857	4.86234150169303E-5	3.740262693610023E-6	4.4883152323320275E-5	1.4961050774440092E-5
64-65	0.0867628737036717	4.86234150169303E-5	3.740262693610023E-6	5.049354636373531E-5	1.4961050774440092E-5
66-67	0.10000153350770438	4.86234150169303E-5	3.740262693610023E-6	5.236367771054032E-5	2.0571444814855126E-5
68-69	0.11551053276675835	5.236367771054032E-5	3.740262693610023E-6	5.236367771054032E-5	2.2441576161660137E-5
70-71	0.1341987553153808	5.236367771054032E-5	3.740262693610023E-6	5.423380905734534E-5	2.618183885527016E-5
72-73	0.1566197600322261	5.236367771054032E-5	3.740262693610023E-6	5.6103940404150345E-5	2.618183885527016E-5
74-75	0.18272866376497088	5.236367771054032E-5	3.740262693610023E-6	5.6103940404150345E-5	3.3662364242490205E-5
76-77	0.21343622047950916	5.236367771054032E-5	3.740262693610023E-6	5.6103940404150345E-5	3.740262693610023E-5
78-79	0.24906409276749142	5.6103940404150345E-5	3.740262693610023E-6	5.6103940404150345E-5	3.740262693610023E-5
80-81	0.2898086444203322	5.6103940404150345E-5	3.740262693610023E-6	5.6103940404150345E-5	4.114288962971025E-5
82-83	0.3370612531600544	5.6103940404150345E-5	3.740262693610023E-6	5.6103940404150345E-5	4.114288962971025E-5
84-85	0.3921758940817449	5.984420309776037E-5	3.740262693610023E-6	5.6103940404150345E-5	4.114288962971025E-5
86-87	0.4542474236135501	6.171433444456538E-5	3.740262693610023E-6	5.7974071750955354E-5	4.114288962971025E-5
88	0.5064427895028779	6.358446579137039E-5	3.740262693610023E-6	5.984420309776037E-5	4.114288962971025E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	61560	0.0	80.77492	1
TATCTCG	65775	0.0	75.2219	2
ATATGGA	57900	0.0	64.95317	1
TATGGAT	59790	0.0	62.58884	2
ATGGATC	63680	0.0	58.477757	3
ATCTCGA	88415	0.0	55.630665	3
TGGATCC	69025	0.0	53.709354	4
GGATCCG	72505	0.0	50.78771	5
TCTCGAG	96490	0.0	50.61614	4
CTCGAGG	107250	0.0	45.366993	5
TCGAGGG	114705	0.0	42.229603	6
GATCCGG	92925	0.0	39.553543	6
CGAGGGC	133635	0.0	36.218697	7
ATCCGGC	106445	0.0	34.250698	7
GAGGGCG	150930	0.0	32.04643	8
AGGGCGC	151595	0.0	31.821217	9
TCCGGCG	120190	0.0	30.24362	8
CCGGCGC	137160	0.0	26.258503	9
GGCGCGC	315135	0.0	15.21859	10-11
GGGCGCG	164945	0.0	14.739534	10-11
>>END_MODULE