FastQCFastQC Report
Wed 25 May 2016
SRR491006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491006_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36456083
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT4689291.2862846510416381No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT4305801.1810923296394733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1695440.4650636767532047No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1675490.45959133898175514No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT845820.23201066335075002No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT674240.18494581548983197No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT607740.16670468958500012No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT588050.1613036705012988No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550850.15109961210040035No Hit
TATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT462520.12687045945117034No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT454110.12456357420516076No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT452580.12414389115802703No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT446990.12261053937144042No Hit
ATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT432920.11875110115368126No Hit
GCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427280.11720403423483537No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT414110.11359146839774312No Hit
TGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382300.10486590125439423No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380700.10442701702209753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC867500.079.004291
TATCTCG882400.076.851332
ATATGGA949100.069.7824251
TATGGAT966050.068.176432
ATGGATC1032400.063.6805573
TGGATCC1114150.058.7064824
GGATCCG1103250.058.5933575
ATCTCGA1197750.056.322623
TCTCGAG1305350.051.206354
GATCCGG1337100.048.1025776
CTCGAGG1433900.046.167595
TCGAGGG1519100.043.2371866
ATCCGGC1529100.041.527987
CGAGGGC1742000.037.5942767
TCCGGCG1726950.036.442738
GAGGGCG1909500.034.199718
AGGGCGC1907700.034.0664379
CCGGCGC1982050.031.0526039
GGCGCGC4131800.017.0880410-11
CCGTCGA2114350.016.22442616-17
Produced by FastQC (version 0.11.5)