FastQCFastQC Report
Wed 25 May 2016
SRR491005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33698770
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1325300.3932784490353803No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT718050.2130789936843392No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA674340.2001081938598946No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT512110.15196697090131184No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438230.13004332205596822No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT392680.116526508237541No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361070.10714634391700349No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359450.10666561420491015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGATC417950.046.3382033
TATGGAT430550.045.1893962
ATATCTC244200.045.183871
TATCTCG246800.044.285912
GGATCCG430800.044.2099885
ATATGGA448400.043.894341
TGGATCC461850.041.677214
ATCTCGA338250.036.7937551
TCTCGAG374150.033.1717382
GATCCGG579550.032.7141156
GAGTACG44450.029.17603594
ATTCGTG46450.028.957092
CTCGAGG431500.028.4470253
AATTCGT49150.026.901041
TCGAGGG465100.026.1784254
ATCCGGC738350.025.5160587
GCGTTAC27150.025.09633674-75
ACGTAAC31450.023.38991514-15
CGGATCC1789550.023.15986
CCGGATC1807850.022.9740585
Produced by FastQC (version 0.11.5)