##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491005_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33698770
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.414223011700425	33.0	31.0	34.0	28.0	34.0
2	31.65954712887147	34.0	31.0	34.0	28.0	34.0
3	31.75334699159643	34.0	31.0	34.0	29.0	34.0
4	35.158160075278715	37.0	35.0	37.0	32.0	37.0
5	34.897349517504644	37.0	35.0	37.0	32.0	37.0
6	34.80907650338573	37.0	35.0	37.0	32.0	37.0
7	34.736777751828924	37.0	35.0	37.0	32.0	37.0
8	34.738187447197625	37.0	35.0	37.0	32.0	37.0
9	36.344812970918525	39.0	37.0	39.0	32.0	39.0
10-11	36.3129992133244	39.0	37.0	39.0	32.0	39.0
12-13	36.2399991453694	39.0	37.0	39.0	32.0	39.0
14-15	37.51174771364059	40.0	38.0	41.0	32.0	41.0
16-17	37.46435132498901	40.0	38.0	41.0	32.0	41.0
18-19	37.43548343159112	40.0	38.0	41.0	32.0	41.0
20-21	37.3357339303482	40.0	38.0	41.0	31.0	41.0
22-23	37.206721180031195	40.0	38.0	41.0	31.0	41.0
24-25	36.936351460305524	40.0	37.0	41.0	31.0	41.0
26-27	36.63838140086418	40.0	36.0	41.0	30.0	41.0
28-29	36.376273926318376	40.0	35.0	41.0	30.0	41.0
30-31	36.142521715184266	40.0	35.0	41.0	30.0	41.0
32-33	35.846885628169815	39.5	35.0	41.0	28.5	41.0
34-35	35.47553554625287	39.0	35.0	41.0	26.5	41.0
36-37	34.93100366867989	39.0	35.0	41.0	23.5	41.0
38-39	34.50179012468408	39.0	35.0	41.0	19.5	41.0
40-41	34.17646694523272	39.0	34.5	41.0	15.5	41.0
42-43	34.08642905957696	39.0	35.0	41.0	9.0	41.0
44-45	33.90379607326915	39.0	34.0	41.0	4.5	41.0
46-47	33.66391156116381	39.0	34.0	41.0	2.0	41.0
48-49	33.331413075314025	39.0	33.5	41.0	2.0	41.0
50-51	33.02748174488268	39.0	33.0	41.0	2.0	41.0
52-53	32.77470750416113	38.5	33.0	41.0	2.0	41.0
54-55	32.538197121141216	38.0	33.0	41.0	2.0	41.0
56-57	32.22157555008684	38.0	32.0	40.5	2.0	41.0
58-59	31.893802785680307	37.0	31.5	40.0	2.0	41.0
60-61	31.546961016678058	37.0	31.0	40.0	2.0	41.0
62-63	31.218593527300847	36.0	31.0	40.0	2.0	41.0
64-65	30.812440543082136	36.0	30.5	39.5	2.0	41.0
66-67	30.41246401574894	35.0	30.0	39.0	2.0	41.0
68-69	29.99035770445034	35.0	29.0	39.0	2.0	41.0
70-71	29.543050888800984	35.0	29.0	38.0	2.0	40.0
72-73	29.081052676403324	35.0	28.5	37.0	2.0	39.5
74-75	28.600234222198615	35.0	27.5	37.0	2.0	39.0
76-77	27.87486838540398	34.0	26.5	36.0	2.0	39.0
78-79	27.907895792635756	34.0	27.0	36.0	2.0	38.0
80-81	27.6029685949962	34.0	26.0	35.0	2.0	37.0
82-83	27.21998305872885	34.0	25.5	35.0	2.0	37.0
84-85	26.80799403064266	34.0	24.5	35.0	2.0	36.0
86-87	26.441614100455297	34.0	23.5	35.0	2.0	36.0
88-89	26.062964597817665	34.0	21.0	35.0	2.0	36.0
90-91	25.669063930226535	34.0	19.0	35.0	2.0	35.0
92-93	25.249246292965587	33.0	13.5	35.0	2.0	35.0
94-95	24.82328165093266	33.0	2.0	35.0	2.0	35.0
96-97	24.36403847974273	33.0	2.0	35.0	2.0	35.0
98-99	23.877205028551487	33.0	2.0	35.0	2.0	35.0
100	22.99717330929289	31.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.35389455285578464
1101	2	0.37016396730598444
1101	3	0.32991728260431685
1101	4	0.30968926429945753
1101	5	0.3943784999925768
1101	6	0.4172380583238322
1101	7	0.4472586403081209
1101	8	0.44063174513294
1101	9	0.4824080631114569
1101	10-11	0.4592225040137734
1101	12-13	0.458532409190596
1101	14-15	0.549144987085981
1101	16-17	0.5432619107679599
1101	18-19	0.530795899236999
1101	20-21	0.5677101589619866
1101	22-23	0.5635458743062074
1101	24-25	0.5616972586947213
1101	26-27	0.5489597405031645
1101	28-29	0.5333599015999653
1101	30-31	0.5143411417571926
1101	32-33	0.5407507945027845
1101	34-35	0.516633937347379
1101	36-37	0.489366498771048
1101	38-39	0.4707014623209531
1101	40-41	0.41167185977155185
1101	42-43	0.40444306311972866
1101	44-45	0.4050757459473715
1101	46-47	0.3820936333621745
1101	48-49	0.42388899499226085
1101	50-51	0.3983175423724816
1101	52-53	0.3650793747054166
1101	54-55	0.28046714357903824
1101	56-57	0.2855118527342242
1101	58-59	0.29859662828266664
1101	60-61	0.27046103529572463
1101	62-63	0.22200506658375474
1101	64-65	0.17700520361970362
1101	66-67	0.15370057502103762
1101	68-69	0.15415434663895056
1101	70-71	0.10646935514101585
1101	72-73	0.08676989048051809
1101	74-75	0.10123747432945152
1101	76-77	0.13300426548923028
1101	78-79	0.12745404962038975
1101	80-81	0.1577457429168838
1101	82-83	0.10242638272793414
1101	84-85	0.015476548401981205
1101	86-87	0.0019148156120358806
1101	88-89	0.013786335998144494
1101	90-91	-0.025654191210218613
1101	92-93	-0.053290458728110224
1101	94-95	-0.022916887513257933
1101	96-97	0.009835205544359127
1101	98-99	0.031023149481814727
1101	100	0.09996783738436577
1102	1	0.27867141629406333
1102	2	0.2897324261972045
1102	3	0.2535993672017902
1102	4	0.24509996376936272
1102	5	0.3209629587735634
1102	6	0.3155277216526571
1102	7	0.3415747797210926
1102	8	0.3233328963279263
1102	9	0.3459957905741362
1102	10-11	0.35771392833748905
1102	12-13	0.33329189191179154
1102	14-15	0.4098770170815982
1102	16-17	0.41488275052656576
1102	18-19	0.42774796666679293
1102	20-21	0.4359508076673251
1102	22-23	0.48166137899959693
1102	24-25	0.49346092040062217
1102	26-27	0.4567981813021902
1102	28-29	0.4298686955853199
1102	30-31	0.4429913240909329
1102	32-33	0.46399263604837415
1102	34-35	0.4590176501027514
1102	36-37	0.4073820421312604
1102	38-39	0.3823698106478872
1102	40-41	0.38187536394750055
1102	42-43	0.35242230312940137
1102	44-45	0.39127648719323815
1102	46-47	0.35088570943590724
1102	48-49	0.38193317422797435
1102	50-51	0.3887355120922962
1102	52-53	0.3483592855857438
1102	54-55	0.3409531737640563
1102	56-57	0.3514097434477961
1102	58-59	0.3420224153632745
1102	60-61	0.3417396888796702
1102	62-63	0.27965458415335576
1102	64-65	0.268135466562736
1102	66-67	0.22562591497453255
1102	68-69	0.18825377426567513
1102	70-71	0.20573510317008115
1102	72-73	0.23683598511526327
1102	74-75	0.21556969607727794
1102	76-77	0.19539819052220508
1102	78-79	0.22964851463031266
1102	80-81	0.22289411837204653
1102	82-83	0.16923448525938056
1102	84-85	0.18071467817038922
1102	86-87	0.15882490397862625
1102	88-89	0.14021958516928734
1102	90-91	0.11413707720670274
1102	92-93	0.07208516615847671
1102	94-95	0.1110453134494982
1102	96-97	0.140814528592351
1102	98-99	0.09945568421059292
1102	100	0.16973968063995315
1103	1	0.09202018399737355
1103	2	0.08846436445882233
1103	3	0.08393861642795031
1103	4	0.07400018705451572
1103	5	0.06774223904202614
1103	6	0.07856599796176766
1103	7	0.09747081938645152
1103	8	0.06653444520768659
1103	9	0.08241440393470612
1103	10-11	0.07787317634657853
1103	12-13	0.1020075348502516
1103	14-15	0.15067201112078266
1103	16-17	0.11157684336307483
1103	18-19	0.11420389760988314
1103	20-21	0.01932765668403391
1103	22-23	0.10437054314105865
1103	24-25	0.10741576606126557
1103	26-27	0.1264589437658188
1103	28-29	0.1325805922069776
1103	30-31	0.11882196544053159
1103	32-33	0.11567233506224284
1103	34-35	0.045781048182632844
1103	36-37	-0.019994851724028706
1103	38-39	0.05082670746739382
1103	40-41	0.07110739403589861
1103	42-43	0.03666961944780667
1103	44-45	0.06120477292620308
1103	46-47	0.03928601299399759
1103	48-49	0.020782646545619343
1103	50-51	0.07269409184915077
1103	52-53	0.0496279700220299
1103	54-55	-0.013474727875681936
1103	56-57	-0.03368776654452432
1103	58-59	-0.03521065365668363
1103	60-61	0.008194501988569414
1103	62-63	0.01322699154755469
1103	64-65	-0.008840940693101373
1103	66-67	-0.02975588987837341
1103	68-69	-0.03862871620608033
1103	70-71	-0.023342677944828694
1103	72-73	-0.08137184310941592
1103	74-75	-0.08371022488486446
1103	76-77	-0.05115453291975314
1103	78-79	-0.03398186089636468
1103	80-81	-0.02979472156380325
1103	82-83	-0.010097024932271381
1103	84-85	-0.0970312785866767
1103	86-87	-0.12115586855637162
1103	88-89	-0.05958519333571033
1103	90-91	-0.0905013886933439
1103	92-93	-0.14039587355104644
1103	94-95	-0.19886257991338496
1103	96-97	-0.14930020119082954
1103	98-99	-0.08428049238424151
1103	100	-0.027768677100173278
1104	1	0.005196792842976805
1104	2	0.04038720624313541
1104	3	0.03396486996014758
1104	4	0.015902166998230882
1104	5	-0.03198087089033663
1104	6	-0.005469962365360459
1104	7	0.03326728491398967
1104	8	0.015433560837564642
1104	9	0.03781762724158
1104	10-11	-0.005628257952785987
1104	12-13	0.03638078098436637
1104	14-15	0.03311305151325428
1104	16-17	0.034229216586915356
1104	18-19	0.014175429045039323
1104	20-21	0.0019241930549469544
1104	22-23	-0.022293899389552507
1104	24-25	0.07061157761150127
1104	26-27	0.04759206406127703
1104	28-29	0.04396520991845421
1104	30-31	0.011196304137044422
1104	32-33	0.04420922532442262
1104	34-35	0.036817303217013375
1104	36-37	-0.0016187472482229737
1104	38-39	0.05839209712438276
1104	40-41	0.03326220439796401
1104	42-43	0.031205522472490088
1104	44-45	-0.01931368475641193
1104	46-47	0.009679507402701404
1104	48-49	0.10942054839811277
1104	50-51	0.1339971014012491
1104	52-53	0.06672853343019369
1104	54-55	0.02825413735893534
1104	56-57	0.03421255745334406
1104	58-59	0.04140521040242362
1104	60-61	-0.009237011821205954
1104	62-63	-0.0523193633038872
1104	64-65	0.0014304258404251868
1104	66-67	-0.05217648975732203
1104	68-69	-0.05799812705726737
1104	70-71	-0.03228995397595824
1104	72-73	-0.01094574220375577
1104	74-75	-0.0679139521573795
1104	76-77	-0.01948412190618498
1104	78-79	-0.024514438990280496
1104	80-81	-0.03851799991712568
1104	82-83	-0.04428732437867211
1104	84-85	-0.026359760990668946
1104	86-87	-0.005855522705608962
1104	88-89	-0.040299018369172046
1104	90-91	-0.06631022797397534
1104	92-93	-0.08456775450069642
1104	94-95	-0.04792693746913912
1104	96-97	-0.058474183655153666
1104	98-99	-0.017426542451115523
1104	100	0.02299296825150421
1105	1	-0.11179285030873132
1105	2	-0.15896494153152574
1105	3	-0.1426637810906044
1105	4	-0.1469451868747953
1105	5	-0.15995726018859813
1105	6	-0.15916821842301943
1105	7	-0.10574878874758298
1105	8	-0.150619490015508
1105	9	-0.20522454118379585
1105	10-11	-0.20065099601649905
1105	12-13	-0.19556601383943217
1105	14-15	-0.16704527595756957
1105	16-17	-0.13784690179472392
1105	18-19	-0.22825574676570426
1105	20-21	-0.18353400411966447
1105	22-23	-0.1444079459678207
1105	24-25	-0.12522915682066582
1105	26-27	-0.14551723150505325
1105	28-29	-0.22745350207796378
1105	30-31	-0.21308816633668926
1105	32-33	-0.10945193684027288
1105	34-35	-0.11511696318800091
1105	36-37	-0.10883840843651171
1105	38-39	-0.12409164054458444
1105	40-41	-0.11437577030479673
1105	42-43	-0.16266430719576164
1105	44-45	-0.04406648475337249
1105	46-47	-0.0775694998233547
1105	48-49	-0.07033481211866643
1105	50-51	-0.037976080111079114
1105	52-53	-0.014029422893216292
1105	54-55	-0.05128965245123851
1105	56-57	-0.017061464054755504
1105	58-59	-0.017338228792436183
1105	60-61	-0.07885299444581761
1105	62-63	-0.06377289012241505
1105	64-65	-0.06283230143150931
1105	66-67	-0.06236813422164644
1105	68-69	-0.17534139884428868
1105	70-71	-0.18198325970891105
1105	72-73	-0.048371996677676776
1105	74-75	-0.06004874983229769
1105	76-77	-0.035363158305155906
1105	78-79	-0.03019584436173517
1105	80-81	-0.06323716219412034
1105	82-83	-0.07612492753761302
1105	84-85	-0.10162661260363137
1105	86-87	-0.06114294359162642
1105	88-89	-0.039000314165988925
1105	90-91	-0.0023814599989151475
1105	92-93	-0.016109205828239936
1105	94-95	-0.11182806510182175
1105	96-97	-0.05106555436930549
1105	98-99	-0.0023144451987349157
1105	100	0.054714235599345074
1106	1	-0.3511038013711527
1106	2	-0.29672313277687934
1106	3	-0.30865037315943056
1106	4	-0.2842572639814023
1106	5	-0.3154537510873112
1106	6	-0.4402989073759045
1106	7	-0.4190693798217566
1106	8	-0.3979199352520695
1106	9	-0.49472913252174777
1106	10-11	-0.487901458029377
1106	12-13	-0.5108056590110621
1106	14-15	-0.5305333742789884
1106	16-17	-0.540473093195402
1106	18-19	-0.5633175341343275
1106	20-21	-0.5996402171739135
1106	22-23	-0.5910319018243868
1106	24-25	-0.5745309618294314
1106	26-27	-0.5867332432825947
1106	28-29	-0.49704254893685373
1106	30-31	-0.5049097446515276
1106	32-33	-0.5150782800336771
1106	34-35	-0.5397591470410177
1106	36-37	-0.5670546157236629
1106	38-39	-0.5835659408392146
1106	40-41	-0.4917077767567761
1106	42-43	-0.4678782484114379
1106	44-45	-0.4123800274834366
1106	46-47	-0.43425893215093936
1106	48-49	-0.4306541363928389
1106	50-51	-0.3929774856063233
1106	52-53	-0.40026060552631293
1106	54-55	-0.36620721675563317
1106	56-57	-0.4211109556263679
1106	58-59	-0.4045973902975035
1106	60-61	-0.3613799474722228
1106	62-63	-0.3720006190280074
1106	64-65	-0.42390287612307276
1106	66-67	-0.32712827413557477
1106	68-69	-0.3416371617670819
1106	70-71	-0.37121277131216246
1106	72-73	-0.345051368922185
1106	74-75	-0.3570436622623454
1106	76-77	-0.3886082372794455
1106	78-79	-0.36865346872980354
1106	80-81	-0.40456739659082075
1106	82-83	-0.4514730954969792
1106	84-85	-0.42397584587132897
1106	86-87	-0.42930140620066837
1106	88-89	-0.48727577287760937
1106	90-91	-0.445992341051344
1106	92-93	-0.44909496627168366
1106	94-95	-0.47161604854574435
1106	96-97	-0.36382009256903913
1106	98-99	-0.3064226479823269
1106	100	-0.37412262171142885
1107	1	-0.5169674141550331
1107	2	-0.47536972684458334
1107	3	-0.4720946944467421
1107	4	-0.43910301814894126
1107	5	-0.5085994999152632
1107	6	-0.6501365681468343
1107	7	-0.7038122705758454
1107	8	-0.6835919164856321
1107	9	-0.6840828363079439
1107	10-11	-0.7152888368843051
1107	12-13	-0.6859035999668279
1107	14-15	-0.749619283350853
1107	16-17	-0.7815303656296706
1107	18-19	-0.8383056901548969
1107	20-21	-0.7321604090962666
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2207	84-85	0.22097554106031936
2207	86-87	0.24172453846190578
2207	88-89	0.2623163335636214
2207	90-91	0.31166486503098056
2207	92-93	0.2849825070877081
2207	94-95	0.2686456237583741
2207	96-97	0.3082596104005937
2207	98-99	0.388060334313554
2207	100	0.39714458177576617
2208	1	-0.6627209033601353
2208	2	-0.5334053377001204
2208	3	-0.4862483907802364
2208	4	-0.4488921987528798
2208	5	-0.5284788937302309
2208	6	-0.4515466818868745
2208	7	-0.4362088322740689
2208	8	-0.48388561586924794
2208	9	-0.5983728031788544
2208	10-11	-0.6071094936745354
2208	12-13	-0.6820155039384375
2208	14-15	-0.8281858353316665
2208	16-17	-0.7919950713741386
2208	18-19	-0.7761304824197879
2208	20-21	-0.7098368868971434
2208	22-23	-1.5538895985334378
2208	24-25	-0.8219459530739641
2208	26-27	-0.7181978258238786
2208	28-29	-0.6889239201324528
2208	30-31	-0.7180787294247892
2208	32-33	-0.7674720483304043
2208	34-35	-0.8069679563966332
2208	36-37	-0.7509249296360991
2208	38-39	-0.7086920327737118
2208	40-41	-1.064305187052632
2208	42-43	-0.9037874466943023
2208	44-45	-1.3615188347346958
2208	46-47	-0.9080301144366629
2208	48-49	-0.8713751175416249
2208	50-51	-1.142965838723292
2208	52-53	-0.7301947484351032
2208	54-55	-0.6308821688908921
2208	56-57	-0.6156062165837355
2208	58-59	-0.5225033998369284
2208	60-61	-0.4941494858953597
2208	62-63	-0.49900594104266816
2208	64-65	-0.43240085362174696
2208	66-67	-0.5934193455417933
2208	68-69	-0.5338357619923499
2208	70-71	-0.5329543412404298
2208	72-73	-0.6353383601241518
2208	74-75	-0.4561155513943582
2208	76-77	-0.4126684560662248
2208	78-79	-0.3740142635597081
2208	80-81	-0.2749827992427356
2208	82-83	-0.33929060458780214
2208	84-85	-0.22850809631875535
2208	86-87	-0.21678786010916085
2208	88-89	-0.4552507080786512
2208	90-91	-0.10840055675679494
2208	92-93	-0.04011204276800484
2208	94-95	-0.04685105113908605
2208	96-97	-0.3889571273502135
2208	98-99	-0.6700490147747722
2208	100	-0.5258509340991786
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	569800.0
3	190484.0
4	105197.0
5	87203.0
6	95182.0
7	109294.0
8	119110.0
9	125674.0
10	134059.0
11	147143.0
12	196617.0
13	363039.0
14	621459.0
15	530874.0
16	439727.0
17	361946.0
18	327405.0
19	279938.0
20	249246.0
21	247051.0
22	260101.0
23	281781.0
24	315544.0
25	357462.0
26	407915.0
27	467300.0
28	534646.0
29	613623.0
30	716814.0
31	838576.0
32	996719.0
33	1239490.0
34	1637584.0
35	2257316.0
36	3454936.0
37	5669386.0
38	6545549.0
39	1797812.0
40	5768.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.28995004723961	27.368788549786483	26.299291177202015	24.041970225771887
2	24.520081619534317	26.216264462486162	26.16827269863377	23.095381219345747
3	24.63017124351476	25.712482868481484	25.309048424330616	24.348297463673138
4	24.827453050088295	24.435923834567653	26.140020090018094	24.596603025325958
5	25.064827017576636	24.696051787878012	24.88931206968567	25.34980912485968
6	25.42298910519057	24.796154444704477	24.54934620451735	25.231510245587607
7	25.957192679620455	25.74165089601071	24.82175296093057	23.479403463438263
8	25.420351001160572	25.829660396099563	25.618237136844314	23.13175146589555
9	23.80156672631234	26.141557880414577	26.107613766820332	23.94926162645275
10-11	23.632041417496115	25.272302845089754	26.149225244002	24.946430493412127
12-13	22.835720738232553	25.133419245760063	27.409804160605066	24.621055855402318
14-15	22.366797496290804	25.185395972594982	29.553424715150197	22.89438181596402
16-17	22.148550082137582	25.158420068416028	30.087344388745414	22.60568546070098
18-19	21.65101527213474	25.12841883108014	31.146951095676794	22.073614801108327
20-21	21.148569551903343	24.856128916114198	32.32096064745464	21.674340884527815
22-23	21.109400776817047	24.983223607756084	32.548504242735014	21.35887137269185
24-25	20.765375005773393	24.552247396392676	33.2998283595478	21.382549238286128
26-27	20.764495678020463	24.577183973335796	33.81826461219143	20.840055736452314
28-29	20.82796005322289	24.708060917318946	33.69021570482113	20.773763324637038
30-31	20.882276869205363	24.660066327073	33.60318646709155	20.854470336630087
32-33	20.89920630386023	24.744383445324715	33.47075391892636	20.88565633188869
34-35	21.07725252890959	24.869770494193723	33.111786864757995	20.94119011213869
36-37	21.333671512715473	25.135534332155807	32.277788784752474	21.253005370376254
38-39	21.634619994649825	25.4969091326165	31.30925117413952	21.559219698594156
40-41	21.818485296858157	25.882278074246173	30.509978592449034	21.789258036446636
42-43	21.937805286134246	26.027693384813848	29.862453501585918	22.17204782746599
44-45	22.137561006484834	26.228090420282268	29.304665901360533	22.329682671872362
46-47	22.375247184531652	26.464885195012638	28.795779167485303	22.364088452970407
48-49	22.466183646287988	26.697178528406702	28.306939249113043	22.52969857619227
50-51	22.538841505256702	26.921998781637953	27.94603735127883	22.593122361826516
52-53	22.51554300162188	27.064849969715915	27.774117576807733	22.645489451854477
54-55	22.604734234203356	27.1510421067098	27.594197533601005	22.65002612548584
56-57	22.584335172163133	27.278149281460507	27.544428730441222	22.593086815935134
58-59	22.606714177890517	27.523767423315448	27.392054716592313	22.477463682201723
60-61	22.62582633463411	27.516570469641895	27.275148698460534	22.582454497263466
62-63	22.727830852409433	27.675251303042785	27.100345990776436	22.496571853771343
64-65	22.673424348354484	27.859758072324553	27.048797688380212	22.41801989094075
66-67	22.692359515281236	27.99491253640421	26.876354631411154	22.436373316903396
68-69	22.652610988987245	28.223604580091138	26.679295322376635	22.444489108544982
70-71	22.729719499500813	28.336938009668383	26.565723560182136	22.367618930648668
72-73	22.67539285492631	28.456010752423595	26.357896994486513	22.510699398163585
74-75	22.75462729274862	28.584843498184924	26.25035538797252	22.410173821093938
76-77	22.549846677767036	28.871067743134653	26.04653222123116	22.532553357867148
78-79	22.632963201974658	28.87504753333239	25.991468007952896	22.500521256740058
80-81	22.607654593440554	29.033735749138167	25.800907028051522	22.557702629369754
82-83	22.638950176921295	29.11446821888399	25.678396383676432	22.568185220518284
84-85	22.573800406746905	29.138753637975075	25.491653022869055	22.79579293240897
86-87	22.59372986318043	29.31065062228638	25.418763124103876	22.67685639042931
88-89	22.701426985181016	29.3987531626171	25.205767459771227	22.69405239243066
90-91	22.684679514732515	29.40957270822156	25.065048706754506	22.84069907029142
92-93	22.66091192650594	29.48145626994117	25.022353190997304	22.835278612555584
94-95	22.704235582242994	29.51938307338721	24.941991679444968	22.834389664924828
96-97	22.65971797484752	29.63580536080947	24.75152412820128	22.95295253614173
98-99	22.68051453948895	29.62721993107965	24.692359098144795	22.9999064312866
100	23.009430184334978	29.5278438741192	24.10511620550404	23.357609736041784
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16229.0
1	9727.0
2	3276.5
3	3321.5
4	3389.0
5	3758.0
6	4429.0
7	5244.5
8	6454.5
9	8772.0
10	13442.0
11	19651.0
12	23423.5
13	29309.5
14	48519.5
15	62587.0
16	101648.5
17	120675.5
18	95896.0
19	89127.0
20	90621.5
21	98171.0
22	110178.5
23	126593.5
24	144845.5
25	166193.5
26	192370.0
27	224351.0
28	260875.5
29	299452.5
30	338508.5
31	387261.5
32	449044.0
33	509535.0
34	561026.0
35	629060.5
36	720084.5
37	819673.5
38	916117.5
39	993902.5
40	1065069.0
41	1150858.5
42	1272786.5
43	1373822.5
44	1447914.0
45	1508395.0
46	1487740.5
47	1386992.0
48	1289530.0
49	1270785.0
50	1262653.5
51	1206701.0
52	1109692.0
53	998697.0
54	910968.5
55	838765.5
56	751185.0
57	662989.5
58	599265.5
59	533176.5
60	459157.0
61	390379.0
62	335460.5
63	291645.5
64	254338.5
65	219712.0
66	188309.0
67	158947.0
68	129619.0
69	103805.5
70	82044.5
71	64078.5
72	49707.0
73	38360.5
74	29352.0
75	21994.5
76	16550.0
77	12336.0
78	8885.5
79	6132.5
80	4025.0
81	2589.0
82	1663.0
83	1075.0
84	682.0
85	455.0
86	315.0
87	185.5
88	117.5
89	74.0
90	43.5
91	32.5
92	25.0
93	19.0
94	11.5
95	8.5
96	6.5
97	3.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07643008928812535
2	0.01010422635603614
3	0.008418704896350816
4	0.007222815550834645
5	0.032763807106312784
6	0.08216917115965953
7	0.06461066679881788
8	0.08426717058219038
9	0.058999186023703534
10-11	0.042320535734687054
12-13	0.04063501427500173
14-15	0.025269468292166152
16-17	0.03018507797168858
18-19	0.05676765057003564
20-21	0.03384841642588142
22-23	0.023221915814731514
24-25	0.028909067007490186
26-27	0.034600669401286754
28-29	0.08284723745109986
30-31	0.06893426674029943
32-33	0.0476085625677139
34-35	0.03786339976206846
36-37	0.031260784889181416
38-39	0.04121515414360821
40-41	0.022613585006218326
42-43	0.023253074221996826
44-45	0.04346152693406911
46-47	0.0360413748038875
48-49	0.024529085186195225
50-51	0.02996400165347281
52-53	0.013371407917855757
54-55	0.00530879910453705
56-57	0.0405637950583953
58-59	0.028302219932656296
60-61	0.01557030123057904
62-63	0.006977999493750068
64-65	0.011224445283907987
66-67	0.012404013558951855
68-69	0.01570383726171608
70-71	0.02043694769868455
72-73	0.021926616312702214
74-75	0.014717154364981272
76-77	0.002335396811218926
78-79	0.004047625477131658
80-81	0.02790606304028307
82-83	0.005954223255032751
84-85	0.006350380147405973
86-87	0.005773207746158094
88-89	0.005917129913050239
90-91	0.007933523983219566
92-93	0.004983861428770249
94-95	0.003020881771055739
96-97	0.014107339822788783
98-99	0.004774654979988884
100	1.9288537830905995E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.369877E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.37008471873428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40610282137195	30.845208439271083
2	12.36685584770114	9.985020365521391
3	4.435985621787354	5.37243346087928
4	2.025497646584049	3.270780463607799
5	1.1669708287409817	2.3555355610282493
6	0.7022615111271998	1.701021401934685
7	0.47030551846490265	1.329039153688146
8	0.3339097785306392	1.0783972838156566
9	0.24942113641445277	0.9062237166924996
>10	1.4998738815163237	11.732659199986298
>50	0.16434663001232047	4.6201544667534975
>100	0.14680372422961618	13.48297805288251
>500	0.02342593285161013	6.137787879269103
>1k	0.007973653150547343	4.626768899190994
>5k	6.63668792869954E-5	0.19777231047316393
>10k+	1.9910063760990545E-4	2.358219345005588
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	132530	0.3932784490353803	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	71805	0.2130789936843392	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67434	0.2001081938598946	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51211	0.15196697090131184	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43823	0.13004332205596822	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39268	0.116526508237541	No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36107	0.10714634391700349	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35945	0.10666561420491015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.186986943440369E-5	0.0	0.0	0.0	2.9674673586009224E-6
2	1.186986943440369E-5	0.0	0.0	0.0	2.9674673586009224E-6
3	1.4837336793004611E-5	2.9674673586009224E-6	0.0	0.0	2.9674673586009224E-6
4	1.4837336793004611E-5	2.9674673586009224E-6	0.0	0.0	2.9674673586009224E-6
5	1.4837336793004611E-5	2.9674673586009224E-6	0.0	0.0	2.9674673586009224E-6
6	1.4837336793004611E-5	2.9674673586009224E-6	2.9674673586009224E-6	0.0	2.9674673586009224E-6
7	2.373973886880738E-5	2.9674673586009224E-6	2.9674673586009224E-6	0.0	2.9674673586009224E-6
8	2.67072062274083E-5	2.9674673586009224E-6	5.934934717201845E-6	0.0	2.9674673586009224E-6
9	3.857707566181199E-5	2.9674673586009224E-6	5.934934717201845E-6	0.0	2.9674673586009224E-6
10-11	6.825174924782121E-5	2.9674673586009224E-6	5.934934717201845E-6	1.4837336793004612E-6	2.9674673586009224E-6
12-13	1.2760109641983964E-4	2.9674673586009224E-6	5.934934717201845E-6	5.934934717201845E-6	2.9674673586009224E-6
14-15	2.537184591603789E-4	5.934934717201845E-6	5.934934717201845E-6	5.934934717201845E-6	5.934934717201845E-6
16-17	4.8814838048985175E-4	8.902402075802768E-6	5.934934717201845E-6	8.902402075802768E-6	5.934934717201845E-6
18-19	8.754028707872721E-4	1.186986943440369E-5	5.934934717201845E-6	1.038613575510323E-5	5.934934717201845E-6
20-21	0.0014110307290147385	1.7804804151605535E-5	5.934934717201845E-6	1.186986943440369E-5	5.934934717201845E-6
22-23	0.0020119428691314253	2.0772271510206455E-5	5.934934717201845E-6	1.186986943440369E-5	5.934934717201845E-6
24-25	0.0027493585077437545	2.0772271510206455E-5	8.902402075802768E-6	1.335360311370415E-5	5.934934717201845E-6
26-27	0.0035757981671141114	2.373973886880738E-5	8.902402075802768E-6	1.4837336793004611E-5	7.418668396502306E-6
28-29	0.0044882943798838955	2.67072062274083E-5	8.902402075802768E-6	1.6321070472305073E-5	1.038613575510323E-5
30-31	0.005293961767744045	2.67072062274083E-5	8.902402075802768E-6	2.0772271510206455E-5	1.186986943440369E-5
32-33	0.006098145421924895	2.67072062274083E-5	8.902402075802768E-6	2.0772271510206455E-5	1.186986943440369E-5
34-35	0.006989869363184472	2.67072062274083E-5	8.902402075802768E-6	2.67072062274083E-5	1.186986943440369E-5
36-37	0.00781779275623413	2.67072062274083E-5	8.902402075802768E-6	3.115840726530968E-5	1.186986943440369E-5
38-39	0.008632362546170082	2.67072062274083E-5	8.902402075802768E-6	3.2642140944610146E-5	1.186986943440369E-5
40-41	0.00948996061280575	2.67072062274083E-5	8.902402075802768E-6	3.2642140944610146E-5	1.335360311370415E-5
42-43	0.010466257373785454	3.115840726530968E-5	8.902402075802768E-6	3.2642140944610146E-5	1.4837336793004611E-5
44-45	0.011524159487126682	3.412587462391061E-5	8.902402075802768E-6	3.560960830321107E-5	1.4837336793004611E-5
46-47	0.012984153427558334	4.4512010379013834E-5	8.902402075802768E-6	3.560960830321107E-5	1.4837336793004611E-5
48-49	0.014740894103850082	4.4512010379013834E-5	8.902402075802768E-6	3.560960830321107E-5	1.4837336793004611E-5
50-51	0.016973913291197275	4.599574405831429E-5	8.902402075802768E-6	3.560960830321107E-5	1.4837336793004611E-5
52-53	0.019800425950264655	4.747947773761476E-5	8.902402075802768E-6	3.560960830321107E-5	1.4837336793004611E-5
54-55	0.023532016153705315	4.747947773761476E-5	8.902402075802768E-6	3.709334198251153E-5	1.4837336793004611E-5
56-57	0.02820577724350177	4.747947773761476E-5	8.902402075802768E-6	4.0060809341112445E-5	1.4837336793004611E-5
58-59	0.03452351524996313	5.34144124548166E-5	8.902402075802768E-6	4.154454302041291E-5	2.0772271510206455E-5
60-61	0.04292293160848304	5.34144124548166E-5	8.902402075802768E-6	4.154454302041291E-5	2.0772271510206455E-5
62-63	0.053697805587562986	6.231681453061936E-5	8.902402075802768E-6	4.154454302041291E-5	2.0772271510206455E-5
64-65	0.0672309404764625	6.676801556852075E-5	8.902402075802768E-6	4.154454302041291E-5	2.0772271510206455E-5
66-67	0.08444670235738574	6.825174924782121E-5	8.902402075802768E-6	4.747947773761476E-5	2.373973886880738E-5
68-69	0.10605876713007625	7.121921660642214E-5	8.902402075802768E-6	5.0446945096215675E-5	2.67072062274083E-5
70-71	0.13275855468908804	7.715415132362397E-5	1.186986943440369E-5	5.9349347172018446E-5	2.67072062274083E-5
72-73	0.16556242260474197	8.160535236152536E-5	1.186986943440369E-5	5.9349347172018446E-5	2.67072062274083E-5
74-75	0.20584727573142877	8.308908604082582E-5	1.186986943440369E-5	6.231681453061936E-5	2.9674673586009223E-5
76-77	0.2544781308041807	8.754028707872721E-5	1.335360311370415E-5	6.231681453061936E-5	3.2642140944610146E-5
78-79	0.3113540939328053	8.902402075802767E-5	1.4837336793004611E-5	6.973548292712168E-5	3.560960830321107E-5
80-81	0.37853161999681295	8.902402075802767E-5	1.4837336793004611E-5	7.418668396502306E-5	3.560960830321107E-5
82-83	0.4566071699352825	9.050775443732813E-5	1.4837336793004611E-5	7.418668396502306E-5	3.560960830321107E-5
84-85	0.5479814248413221	9.347522179592904E-5	1.4837336793004611E-5	7.715415132362397E-5	3.560960830321107E-5
86-87	0.6517003439591416	9.495895547522952E-5	1.4837336793004611E-5	7.715415132362397E-5	3.857707566181199E-5
88	0.7380180344861251	9.495895547522952E-5	1.4837336793004611E-5	8.01216186822249E-5	3.857707566181199E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGATC	41795	0.0	46.338203	3
TATGGAT	43055	0.0	45.189396	2
ATATCTC	24420	0.0	45.18387	1
TATCTCG	24680	0.0	44.28591	2
GGATCCG	43080	0.0	44.209988	5
ATATGGA	44840	0.0	43.89434	1
TGGATCC	46185	0.0	41.67721	4
ATCTCGA	33825	0.0	36.793755	1
TCTCGAG	37415	0.0	33.171738	2
GATCCGG	57955	0.0	32.714115	6
GAGTACG	4445	0.0	29.176035	94
ATTCGTG	4645	0.0	28.95709	2
CTCGAGG	43150	0.0	28.447025	3
AATTCGT	4915	0.0	26.90104	1
TCGAGGG	46510	0.0	26.178425	4
ATCCGGC	73835	0.0	25.516058	7
GCGTTAC	2715	0.0	25.096336	74-75
ACGTAAC	3145	0.0	23.389915	14-15
CGGATCC	178955	0.0	23.1598	6
CCGGATC	180785	0.0	22.974058	5
>>END_MODULE