FastQCFastQC Report
Wed 25 May 2016
SRR491004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41041777
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1514890.369109261521498No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT831890.2026934652464No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT652950.15909398854732826No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT517470.12608372195969975No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472820.11520456338915346No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440080.10722732595131053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGATC502800.044.5884363
TATGGAT518500.043.528832
ATATGGA530150.042.7032241
ATATCTC316800.042.5562631
GGATCCG529300.041.4906045
TATCTCG327600.040.4406362
TGGATCC553350.040.215934
ATCTCGA425000.038.3099171
TCTCGAG475800.034.0418932
GATCCGG730600.029.9149196
CTCGAGG543500.029.7234733
TCGAGGG592900.027.0077844
ATCCGGC937250.023.3064377
CGGATCC2271900.021.8314366
CCGGATC2298300.021.6334725
GCGCCGG2321550.021.560612
GCCGGAT2324850.021.4075584
CGCCGGA2327800.021.3955123
GATCCTT2399550.020.7711328
CGAGGGC779600.020.6898355
Produced by FastQC (version 0.11.5)