##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491004_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41041777
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28568144113253	33.0	31.0	34.0	28.0	34.0
2	31.533460624767784	34.0	31.0	34.0	28.0	34.0
3	31.6291012691775	34.0	31.0	34.0	28.0	34.0
4	35.03259013370693	37.0	35.0	37.0	32.0	37.0
5	34.75616150830896	37.0	35.0	37.0	32.0	37.0
6	34.66395655821628	37.0	35.0	37.0	32.0	37.0
7	34.587488183077454	37.0	35.0	37.0	32.0	37.0
8	34.588200652228096	37.0	35.0	37.0	32.0	37.0
9	36.17961281257388	39.0	37.0	39.0	32.0	39.0
10-11	36.146767877521484	39.0	37.0	39.0	32.0	39.0
12-13	36.07273862678997	39.0	37.0	39.0	31.0	39.0
14-15	37.32694836775708	40.0	38.0	41.0	31.0	41.0
16-17	37.27698561882445	40.0	38.0	41.0	31.0	41.0
18-19	37.254905300518544	40.0	38.0	41.0	31.0	41.0
20-21	37.159754059869286	40.0	38.0	41.0	31.0	41.0
22-23	37.03117330908942	40.0	38.0	41.0	31.0	41.0
24-25	36.759448269016225	40.0	37.0	41.0	30.0	41.0
26-27	36.45499640524824	40.0	36.0	41.0	30.0	41.0
28-29	36.18672714877818	40.0	35.0	41.0	29.5	41.0
30-31	35.94738625717888	39.5	35.0	41.0	29.0	41.0
32-33	35.65112380489763	39.0	35.0	41.0	27.5	41.0
34-35	35.27753799500445	39.0	35.0	41.0	25.5	41.0
36-37	34.73390294479696	39.0	35.0	41.0	23.0	41.0
38-39	34.304488082959956	39.0	35.0	41.0	17.0	41.0
40-41	33.9685873006912	39.0	34.0	41.0	11.5	41.0
42-43	33.85579396330719	39.0	34.0	41.0	6.5	41.0
44-45	33.65807636691754	39.0	34.0	41.0	2.0	41.0
46-47	33.407855853804776	39.0	33.5	41.0	2.0	41.0
48-49	33.0682304886555	39.0	33.0	41.0	2.0	41.0
50-51	32.76161478095844	39.0	33.0	41.0	2.0	41.0
52-53	32.50825193314607	38.5	33.0	41.0	2.0	41.0
54-55	32.272504721225886	38.0	32.0	41.0	2.0	41.0
56-57	31.96019935978893	38.0	32.0	40.5	2.0	41.0
58-59	31.636701731993718	37.0	31.0	40.0	2.0	41.0
60-61	31.295334117721072	37.0	31.0	40.0	2.0	41.0
62-63	30.97285417246919	36.0	30.5	40.0	2.0	41.0
64-65	30.572446960568982	36.0	30.0	39.5	2.0	41.0
66-67	30.17731548270924	35.0	29.0	39.0	2.0	41.0
68-69	29.759778420412935	35.0	29.0	39.0	2.0	41.0
70-71	29.3153781572372	35.0	28.5	38.0	2.0	40.0
72-73	28.857405467604387	35.0	28.0	37.0	2.0	40.0
74-75	28.378899931647695	35.0	26.5	37.0	2.0	39.0
76-77	27.655996254743066	34.0	26.0	36.0	2.0	39.0
78-79	27.694691630920367	34.0	26.0	36.0	2.0	38.5
80-81	27.388516462140515	34.0	25.5	35.0	2.0	37.0
82-83	27.00534948815691	34.0	25.0	35.0	2.0	37.0
84-85	26.5907357398292	34.0	24.0	35.0	2.0	36.0
86-87	26.2250382336028	34.0	21.5	35.0	2.0	36.0
88-89	25.8472067620269	34.0	19.5	35.0	2.0	36.0
90-91	25.45364131041402	33.5	16.5	35.0	2.0	35.0
92-93	25.035767603337447	33.0	4.5	35.0	2.0	35.0
94-95	24.61262922411961	33.0	2.0	35.0	2.0	35.0
96-97	24.156592720144648	33.0	2.0	35.0	2.0	35.0
98-99	23.67049937920573	33.0	2.0	35.0	2.0	35.0
100	22.799617472703485	31.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.41336110873204035
1101	2	0.43131103639654356
1101	3	0.3886710963130504
1101	4	0.34500239002261424
1101	5	0.4485170774372236
1101	6	0.4779335359106369
1101	7	0.5033841410715709
1101	8	0.4898718708506138
1101	9	0.541285262157281
1101	10-11	0.5213995953855246
1101	12-13	0.5135310404454003
1101	14-15	0.5925377413881492
1101	16-17	0.58292350055806
1101	18-19	0.59200649281388
1101	20-21	0.62578571441653
1101	22-23	0.6468493010817014
1101	24-25	0.6258449567671676
1101	26-27	0.596588426529685
1101	28-29	0.5827136957890957
1101	30-31	0.5604985856664655
1101	32-33	0.6071031901694326
1101	34-35	0.5652515213053135
1101	36-37	0.5751492961048044
1101	38-39	0.5399793970908746
1101	40-41	0.4742362876925057
1101	42-43	0.4577888732956694
1101	44-45	0.4649446139013165
1101	46-47	0.4403155308197668
1101	48-49	0.49443403283441256
1101	50-51	0.481891359297137
1101	52-53	0.44630676853006435
1101	54-55	0.38757180968558913
1101	56-57	0.39452589044118014
1101	58-59	0.39687202976097424
1101	60-61	0.36646285426484226
1101	62-63	0.32263996053778854
1101	64-65	0.285167351434783
1101	66-67	0.268921240924918
1101	68-69	0.22990193137149362
1101	70-71	0.2011858025216391
1101	72-73	0.17496164508247247
1101	74-75	0.18691162794862493
1101	76-77	0.2205602103254236
1101	78-79	0.20397972952757826
1101	80-81	0.22990131638250588
1101	82-83	0.2078344247606445
1101	84-85	0.09258402236771701
1101	86-87	0.08488231444281169
1101	88-89	0.06927113274329244
1101	90-91	0.056527890354704624
1101	92-93	0.014314678305527906
1101	94-95	0.06491557967163786
1101	96-97	0.05156062973732034
1101	98-99	0.11589365498853965
1101	100	0.16835566401712754
1102	1	0.3116053812519901
1102	2	0.31554798700727105
1102	3	0.27895226359790826
1102	4	0.2784440990446839
1102	5	0.35773135265672806
1102	6	0.3350881462357478
1102	7	0.3764418151213036
1102	8	0.35133060665918237
1102	9	0.3884530935738866
1102	10-11	0.3825592093222596
1102	12-13	0.36812791957355273
1102	14-15	0.43463324371317924
1102	16-17	0.46005060025562017
1102	18-19	0.442691610425463
1102	20-21	0.45847579396680516
1102	22-23	0.509169971629909
1102	24-25	0.5137372728763125
1102	26-27	0.47905792625919474
1102	28-29	0.4572216533617137
1102	30-31	0.4654853344058836
1102	32-33	0.4721944159683673
1102	34-35	0.47121959161873406
1102	36-37	0.4129769224374229
1102	38-39	0.3765321843273952
1102	40-41	0.3620256530457908
1102	42-43	0.33352746629725516
1102	44-45	0.3303045394183286
1102	46-47	0.328136284306602
1102	48-49	0.3638135733676364
1102	50-51	0.36910542791135725
1102	52-53	0.3340302058844671
1102	54-55	0.31079640811730513
1102	56-57	0.3240078730293945
1102	58-59	0.3408078789309066
1102	60-61	0.32600112020465843
1102	62-63	0.2734637812623255
1102	64-65	0.26305684957934616
1102	66-67	0.22431480134547144
1102	68-69	0.18772836229634038
1102	70-71	0.21375554588743384
1102	72-73	0.2310509842321551
1102	74-75	0.20119637338183338
1102	76-77	0.18703629019593038
1102	78-79	0.1993480918281776
1102	80-81	0.21304372104900438
1102	82-83	0.16896707329196659
1102	84-85	0.16554667710585136
1102	86-87	0.13726166676539364
1102	88-89	0.13694739969265868
1102	90-91	0.11346908911228226
1102	92-93	0.060988076923727874
1102	94-95	0.08106443982475398
1102	96-97	0.0939735060906699
1102	98-99	0.06756642569698812
1102	100	0.11644036914288236
1103	1	0.1258979067815318
1103	2	0.10043796351875045
1103	3	0.11638078044427047
1103	4	0.11672304775008513
1103	5	0.10250740472142184
1103	6	0.1248903530017742
1103	7	0.14162916810403914
1103	8	0.11723412347399886
1103	9	0.15732425766008618
1103	10-11	0.14430744080764413
1103	12-13	0.14699298943624228
1103	14-15	0.19778002801754724
1103	16-17	0.16778784802564672
1103	18-19	0.1653514719209852
1103	20-21	0.04961330407515874
1103	22-23	0.1562540572952429
1103	24-25	0.15012261935848414
1103	26-27	0.17890512259220515
1103	28-29	0.18676887149329247
1103	30-31	0.1944071054395522
1103	32-33	0.171164719710319
1103	34-35	0.1121474197637724
1103	36-37	0.0024050787750411473
1103	38-39	0.07584541882147278
1103	40-41	0.10383760210842752
1103	42-43	0.07055299576098406
1103	44-45	0.10021927685492926
1103	46-47	0.09375685026115121
1103	48-49	0.04928265786595176
1103	50-51	0.1107256473885343
1103	52-53	0.07918465283952258
1103	54-55	0.0030835463010490116
1103	56-57	-0.027128995786533494
1103	58-59	-0.0030110247936931955
1103	60-61	0.04170135516495321
1103	62-63	0.028565237861137405
1103	64-65	0.006685078891372598
1103	66-67	-0.004094041354601785
1103	68-69	-0.013510196197067614
1103	70-71	0.0020156807210653938
1103	72-73	-0.044687682412732244
1103	74-75	-0.04931509115971622
1103	76-77	-0.019790721274070222
1103	78-79	-0.0027193132556675437
1103	80-81	-0.02294611720794748
1103	82-83	-0.01823281412253408
1103	84-85	-0.09974903221777964
1103	86-87	-0.09379598250601973
1103	88-89	-0.053067242844363705
1103	90-91	-0.08418068233460829
1103	92-93	-0.13710037169927247
1103	94-95	-0.20122198942874192
1103	96-97	-0.14796543279851804
1103	98-99	-0.08786882341716051
1103	100	-0.03811442394272646
1104	1	0.04515117247230549
1104	2	0.0702474999365954
1104	3	0.05935493527832847
1104	4	0.05844140659163344
1104	5	0.020061478085452222
1104	6	0.03472810551183869
1104	7	0.05952169210256386
1104	8	0.06133049744991581
1104	9	0.07863360928427454
1104	10-11	0.024911270140485442
1104	12-13	0.06148600411133032
1104	14-15	0.06789756553214232
1104	16-17	0.06524964259794075
1104	18-19	0.0430835297079426
1104	20-21	0.028595632382014458
1104	22-23	0.00584278722885756
1104	24-25	0.10500010231950796
1104	26-27	0.07966603453167664
1104	28-29	0.0914573585996834
1104	30-31	0.05792176602358978
1104	32-33	0.08366734877314741
1104	34-35	0.08573670897644803
1104	36-37	0.05330930703411951
1104	38-39	0.0784955542793
1104	40-41	0.0536214661583756
1104	42-43	0.07245135539680803
1104	44-45	0.004525455335532058
1104	46-47	0.04179431331251493
1104	48-49	0.11469449900074125
1104	50-51	0.13171756385213484
1104	52-53	0.08104994412807542
1104	54-55	0.04771168183612673
1104	56-57	0.06141593344231211
1104	58-59	0.05147712881086264
1104	60-61	0.021285442049926218
1104	62-63	-0.028054968404251923
1104	64-65	0.01963275252040475
1104	66-67	-0.022499640322827474
1104	68-69	-0.031558458831725034
1104	70-71	-0.002075811772346725
1104	72-73	0.012870085241392104
1104	74-75	-0.05364131511246484
1104	76-77	0.023870237556565144
1104	78-79	0.0137849278503559
1104	80-81	-0.006106592392651322
1104	82-83	-0.006689174020046096
1104	84-85	0.011756067672536119
1104	86-87	0.03384106064146053
1104	88-89	0.022902684799376516
1104	90-91	-4.3633376565210824E-4
1104	92-93	-0.023525732358422147
1104	94-95	0.014615607072549608
1104	96-97	0.001643054369822039
1104	98-99	0.07386204026899179
1104	100	0.11928081743223018
1105	1	-0.12285574485182238
1105	2	-0.16555003451709283
1105	3	-0.152677136003188
1105	4	-0.1408229457300081
1105	5	-0.1836799949854111
1105	6	-0.168831639302212
1105	7	-0.11139292589972172
1105	8	-0.17379955962386617
1105	9	-0.21119488575064338
1105	10-11	-0.21681747168344856
1105	12-13	-0.21941335975103016
1105	14-15	-0.19143341892356602
1105	16-17	-0.17157819512711825
1105	18-19	-0.2576356312192303
1105	20-21	-0.21686757810181945
1105	22-23	-0.16730778888437214
1105	24-25	-0.15343475168236864
1105	26-27	-0.16315090924472742
1105	28-29	-0.2672742628425766
1105	30-31	-0.2518870578741428
1105	32-33	-0.17833841547412277
1105	34-35	-0.18429665790193894
1105	36-37	-0.15397435126027403
1105	38-39	-0.15982724051724517
1105	40-41	-0.1631280111180189
1105	42-43	-0.2167898066450462
1105	44-45	-0.09157196887637298
1105	46-47	-0.12707558661564633
1105	48-49	-0.12224872665048991
1105	50-51	-0.09099090570066437
1105	52-53	-0.05040174619790605
1105	54-55	-0.08272362061358507
1105	56-57	-0.053274200492765544
1105	58-59	-0.03724789912188342
1105	60-61	-0.11875052434983857
1105	62-63	-0.08539840079005145
1105	64-65	-0.08648138779846803
1105	66-67	-0.08849918171343063
1105	68-69	-0.1849027483142649
1105	70-71	-0.19667000816188818
1105	72-73	-0.0490434627958507
1105	74-75	-0.06548042129615794
1105	76-77	-0.02896468062358437
1105	78-79	-0.022466811407557685
1105	80-81	-0.05852955540147775
1105	82-83	-0.09629224741100018
1105	84-85	-0.12236322854351656
1105	86-87	-0.0908674972535799
1105	88-89	-0.04810166531410687
1105	90-91	-0.04461366116684573
1105	92-93	-0.047500677248983436
1105	94-95	-0.14084185368842483
1105	96-97	-0.07355411132561684
1105	98-99	-0.018229626088142936
1105	100	0.022708128750203116
1106	1	-0.34754619794984265
1106	2	-0.30894000538997446
1106	3	-0.3154314590382441
1106	4	-0.287135348879076
1106	5	-0.31733081192847123
1106	6	-0.46222457337655243
1106	7	-0.41708851612216336
1106	8	-0.40127709276521273
1106	9	-0.4764692708420739
1106	10-11	-0.4967892622104202
1106	12-13	-0.4790020343641288
1106	14-15	-0.4945640568398417
1106	16-17	-0.5049165912432372
1106	18-19	-0.5443511959457226
1106	20-21	-0.5767166847604486
1106	22-23	-0.5679157341970082
1106	24-25	-0.5432559006790001
1106	26-27	-0.5769001355730623
1106	28-29	-0.47962270768880444
1106	30-31	-0.4968281489509252
1106	32-33	-0.502716949292676
1106	34-35	-0.5284585937814583
1106	36-37	-0.56930605205185
1106	38-39	-0.6179129602646327
1106	40-41	-0.5175309697477388
1106	42-43	-0.49091726071506514
1106	44-45	-0.4447358521252127
1106	46-47	-0.4883550720436389
1106	48-49	-0.49937966991105753
1106	50-51	-0.44458566759135465
1106	52-53	-0.4522200946868864
1106	54-55	-0.43001626603587084
1106	56-57	-0.4818292562154056
1106	58-59	-0.45997147247311077
1106	60-61	-0.4154286800957827
1106	62-63	-0.4405461794684129
1106	64-65	-0.4903844104748494
1106	66-67	-0.3922053493057547
1106	68-69	-0.39735615999865104
1106	70-71	-0.44407496057585405
1106	72-73	-0.42996539283909385
1106	74-75	-0.41151426271927605
1106	76-77	-0.4130848049150657
1106	78-79	-0.4008291382244593
1106	80-81	-0.4227707876078881
1106	82-83	-0.4823955229361587
1106	84-85	-0.42921070405387596
1106	86-87	-0.4333158678293394
1106	88-89	-0.503147529918575
1106	90-91	-0.4515326909534956
1106	92-93	-0.46069040806666095
1106	94-95	-0.47869064652126525
1106	96-97	-0.39695538558628485
1106	98-99	-0.3472204971372008
1106	100	-0.4156352414801532
1107	1	-0.5134665017052242
1107	2	-0.4765340546657413
1107	3	-0.48826031252833957
1107	4	-0.4450253362481362
1107	5	-0.5216800771258079
1107	6	-0.6576823310468356
1107	7	-0.7022018596292696
1107	8	-0.6803382112778777
1107	9	-0.6998625821478441
1107	10-11	-0.7252482937101874
1107	12-13	-0.6990468423672809
1107	14-15	-0.7567632119168479
1107	16-17	-0.7710290513110252
1107	18-19	-0.8280785978338514
1107	20-21	-0.7198947822154906
1107	22-23	-0.7066787256779037
1107	24-25	-0.6869580763281959
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2207	86-87	0.20677240369148464
2207	88-89	0.22769130661723835
2207	90-91	0.26098413370329254
2207	92-93	0.2623113706131086
2207	94-95	0.2488888644350986
2207	96-97	0.29035153745297393
2207	98-99	0.3786798085713876
2207	100	0.36988342970227706
2208	1	-0.7066856382367348
2208	2	-0.5697781287799621
2208	3	-0.5330600527329032
2208	4	-0.4878952831383643
2208	5	-0.56728461536823
2208	6	-0.49704070410746226
2208	7	-0.5016260681333051
2208	8	-0.552959812458667
2208	9	-0.6722977706607196
2208	10-11	-0.6605461007281477
2208	12-13	-0.7304143036664072
2208	14-15	-0.8833009199383071
2208	16-17	-0.8535640757597704
2208	18-19	-0.8265590400551233
2208	20-21	-0.7590943739090079
2208	22-23	-1.5909001134736087
2208	24-25	-0.8531884158474554
2208	26-27	-0.7482906754265244
2208	28-29	-0.7253313589962715
2208	30-31	-0.7558641722686374
2208	32-33	-0.804941621245554
2208	34-35	-0.8618760720794327
2208	36-37	-0.824751569402359
2208	38-39	-0.7802214019620521
2208	40-41	-1.132549544901508
2208	42-43	-0.9739053257206294
2208	44-45	-1.4585053794319478
2208	46-47	-0.9979909476417319
2208	48-49	-0.9317314774850374
2208	50-51	-1.2303026584214578
2208	52-53	-0.8231549806566107
2208	54-55	-0.6983053583732364
2208	56-57	-0.6916085547578348
2208	58-59	-0.6199985236085261
2208	60-61	-0.5723290235746497
2208	62-63	-0.5525098153987216
2208	64-65	-0.5078713815042981
2208	66-67	-0.6616223273834727
2208	68-69	-0.5959208010822046
2208	70-71	-0.585455824016293
2208	72-73	-0.6757656308564322
2208	74-75	-0.47164828761822974
2208	76-77	-0.44490676464435097
2208	78-79	-0.42471172844662775
2208	80-81	-0.33950857349250185
2208	82-83	-0.3984571652286135
2208	84-85	-0.27288397695643596
2208	86-87	-0.2712202473173768
2208	88-89	-0.5378685896232902
2208	90-91	-0.16763078005446985
2208	92-93	-0.11778350247016078
2208	94-95	-0.11543740995248086
2208	96-97	-0.4697080644504297
2208	98-99	-0.7685507181743816
2208	100	-0.6107035197274087
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	785674.0
3	254179.0
4	135204.0
5	111604.0
6	120705.0
7	139619.0
8	152213.0
9	160600.0
10	169789.0
11	184948.0
12	247254.0
13	452337.0
14	765820.0
15	664802.0
16	564513.0
17	462971.0
18	415086.0
19	348701.0
20	307232.0
21	303810.0
22	319717.0
23	346848.0
24	387916.0
25	439252.0
26	502138.0
27	570733.0
28	653660.0
29	752785.0
30	876450.0
31	1026847.0
32	1220998.0
33	1516581.0
34	1966662.0
35	2717591.0
36	4152122.0
37	6811144.0
38	7875704.0
39	2153090.0
40	4478.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.023469980677767	27.27532092343001	26.717127168203593	23.98408192768863
2	24.417752425832045	26.045375641037605	26.450777335275394	23.086094597854956
3	24.685205098338784	25.706760843207604	25.396631695100492	24.21140236335312
4	24.77254304048336	24.45988817965059	26.209561410119964	24.558007369746086
5	25.0927385683325	24.72178916966196	25.094164406226138	25.091307855779398
6	25.416254712759205	24.787691734143223	24.732641263774443	25.06341228932313
7	26.025146266955414	25.732778148833766	24.964492322993248	23.277583261217572
8	25.428060204589436	25.80123636244987	25.775374865507413	22.99532856745328
9	23.819437734883884	26.07380132222613	26.254273771671354	23.85248717121863
10-11	23.626179423502375	25.261669430883373	26.224679628746685	24.88747151686757
12-13	22.83164011644623	25.088306578644648	27.547417326525682	24.532635978383436
14-15	22.31327505451096	25.170469022300797	29.73936186501525	22.776894058172992
16-17	22.051805698214416	25.163589764575367	30.307021500167114	22.477583037043107
18-19	21.52616235608791	25.088248928931105	31.485112206663207	21.90047650831778
20-21	20.96751810956047	24.84018269848492	32.701542000610196	21.490757191344407
22-23	20.965945741807854	24.926976698345598	32.982824084982425	21.124253474864123
24-25	20.610507262470545	24.52801408549447	33.76089231659123	21.10058633544375
26-27	20.631700822755846	24.57450096933164	34.22437430469972	20.569423903212787
28-29	20.668892216270866	24.657894387419915	34.13838430258761	20.53482909372161
30-31	20.68647206376575	24.61519008217955	34.09044505149858	20.607892802556123
32-33	20.736127420066886	24.716569918027133	33.89753964386522	20.64976301804076
34-35	20.887344511060363	24.87479229751308	33.5616774926675	20.67618569875906
36-37	21.16783380746905	25.136260804542342	32.68539429633255	21.010511091656056
38-39	21.477391437279014	25.510147557863235	31.6946887873723	21.317772217485455
40-41	21.696532470663804	25.857375875524234	30.914427969710594	21.531663684101364
42-43	21.85181797612673	26.050990716296603	30.201755288975534	21.895436018601135
44-45	22.022540554330718	26.324351962658437	29.590135818207553	22.062971664803293
46-47	22.267292306839572	26.572922607716198	29.0422489682302	22.11753611721403
48-49	22.34306092934396	26.806000414241705	28.531566785945873	22.31937187046847
50-51	22.420013485123516	27.040551107906435	28.138409749845692	22.401025657124357
52-53	22.452716688542143	27.19786885994337	27.914947396934863	22.434467054579628
54-55	22.516091363719003	27.282177449004575	27.752733494472665	22.44899769280375
56-57	22.483292684147077	27.44247187580351	27.67154074616523	22.402694693884186
58-59	22.552018667648216	27.66246589338122	27.508396986937168	22.277118452033395
60-61	22.544636326972984	27.735252465399192	27.36278467413132	22.357326533496508
62-63	22.608130006733486	27.90407827613302	27.204275512071526	22.283516205061975
64-65	22.58564226537571	28.09806533036828	27.111816631577263	22.20447577267875
66-67	22.57348332861683	28.25207654688698	26.92641380019381	22.24802632430238
68-69	22.570253396167054	28.46273069970424	26.745627117317376	22.22138878681133
70-71	22.60526592425319	28.63832334145846	26.609699042050437	22.146711692237915
72-73	22.586249980396715	28.76208505265982	26.390174568617734	22.261490398325734
74-75	22.630401508701638	28.925521381473413	26.275283900795383	22.168793209029566
76-77	22.45737819342223	29.188199483022352	26.057463581525585	22.29695874202983
78-79	22.51507149849515	29.24685703050718	25.94946124621756	22.288610224780115
80-81	22.52330097355946	29.35962943592912	25.76755232445397	22.349517266057447
82-83	22.537437265553546	29.469377266414433	25.64956216234926	22.343623305682762
84-85	22.48782472877567	29.515044867009504	25.498813275721954	22.49831712849287
86-87	22.499271940830486	29.68393880211736	25.383889703583336	22.432899553468822
88-89	22.58654107103315	29.748800001462023	25.191940980692507	22.472717946812324
90-91	22.560753635848872	29.781216428155222	25.047145188148534	22.610884747847365
92-93	22.55479985641734	29.879558670577005	24.968843335140644	22.59679813786501
94-95	22.611601254349427	29.91420600517393	24.8974662023186	22.576726538158038
96-97	22.544042737777247	30.03098588274955	24.682318183837467	22.742653195635736
98-99	22.570995093482033	30.04053339745369	24.617862585089078	22.770608923975203
100	22.889619443827915	29.937949558928693	24.065261355676128	23.107169641567264
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5655.0
1	3610.5
2	1676.0
3	1845.0
4	2101.0
5	2578.0
6	3260.5
7	4053.0
8	5133.5
9	7728.5
10	12704.5
11	18848.0
12	22689.0
13	29078.0
14	43979.5
15	57180.0
16	99299.0
17	119930.5
18	102941.0
19	106381.5
20	114312.0
21	125501.0
22	140247.5
23	159521.0
24	183484.5
25	211287.0
26	246402.0
27	289483.5
28	338969.0
29	392246.5
30	442526.0
31	504100.5
32	581744.5
33	657530.0
34	722311.5
35	809066.5
36	923440.5
37	1045953.0
38	1161693.5
39	1249164.0
40	1329834.0
41	1429118.5
42	1579404.5
43	1707607.5
44	1806890.0
45	1888378.5
46	1856183.5
47	1720910.0
48	1588628.5
49	1556601.5
50	1548123.0
51	1478230.5
52	1346802.5
53	1197578.0
54	1085429.5
55	990496.5
56	870811.5
57	759372.0
58	683052.0
59	601991.5
60	509991.0
61	426711.5
62	362206.0
63	311467.0
64	270070.5
65	232113.0
66	196711.0
67	163842.5
68	132686.0
69	106439.5
70	84488.0
71	66322.5
72	52312.0
73	40813.0
74	31071.0
75	23461.0
76	17636.0
77	13144.5
78	9554.5
79	6626.5
80	4361.0
81	2729.5
82	1739.0
83	1108.0
84	694.5
85	451.0
86	270.0
87	164.5
88	112.0
89	65.0
90	47.5
91	33.5
92	20.0
93	11.5
94	5.0
95	4.5
96	5.5
97	6.0
98	5.5
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07928506604380214
2	0.01025296736055069
3	0.00825987627192653
4	0.007100082435514428
5	0.032332908002497066
6	0.08272789942794143
7	0.06506297229771509
8	0.08496220814220592
9	0.05953933232471879
10-11	0.042632169654837314
12-13	0.04059887562860643
14-15	0.025214551504434128
16-17	0.03037880158064306
18-19	0.05674827383814302
20-21	0.03428092307016823
22-23	0.023558921437539123
24-25	0.028667131055265953
26-27	0.03542487938570496
28-29	0.08339916665889004
30-31	0.06922458547542909
32-33	0.04791946508553955
34-35	0.037880913392224706
36-37	0.031034231290716287
38-39	0.04131278233883489
40-41	0.022285828413326256
42-43	0.023013136102756952
44-45	0.04306587407265528
46-47	0.03758609184977541
48-49	0.024795466336654966
50-51	0.03133636245818499
52-53	0.0130050411803563
54-55	0.00521054436799849
56-57	0.040350348377946696
58-59	0.02786063576145838
60-61	0.01530757306146856
62-63	0.006959981289309184
64-65	0.010868194133017194
66-67	0.01223143919913604
68-69	0.016577011273171724
70-71	0.020353650866530463
72-73	0.02221029562145908
74-75	0.015174781540282721
76-77	0.002268420297688377
78-79	0.004014202406489368
80-81	0.027931295469979283
82-83	0.0059000856614956024
84-85	0.00620708991231057
86-87	0.007001402497752472
88-89	0.006492165288067327
90-91	0.008258658001089964
92-93	0.0048791747004521754
94-95	0.004913286283876062
96-97	0.013815191286673578
98-99	0.005569934264785855
100	0.0027630382573347154
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4.1041777E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.60511430822024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.46417887624648	29.130493014336682
2	11.18745595601052	8.414111200879098
3	4.046437900767251	4.565002793984017
4	2.0417850411129446	3.0712623945546573
5	1.181132283570577	2.220830726840038
6	0.7488798845655372	1.6897028197328283
7	0.5149739663265543	1.3555958408627347
8	0.3697497036261349	1.1123583896233087
9	0.29498635590628275	0.9983696069898992
>10	1.765739191465976	12.812599690082118
>50	0.16312614639425418	4.259817487687508
>100	0.1781498796423266	13.588197087492246
>500	0.032696811910310326	8.448274534576667
>1k	0.01048008424077477	6.0125905536424415
>5k	5.860754398455438E-5	0.16342484763780782
>10k+	1.693106701998728E-4	2.1573690110780417
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	151489	0.369109261521498	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	83189	0.2026934652464	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65295	0.15909398854732826	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51747	0.12608372195969975	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47282	0.11520456338915346	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44008	0.10722732595131053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4365416731346695E-6	0.0	0.0	0.0	0.0
2	2.4365416731346695E-6	0.0	0.0	2.4365416731346695E-6	0.0
3	2.4365416731346695E-6	0.0	0.0	7.309625019404009E-6	0.0
4	2.4365416731346695E-6	0.0	0.0	7.309625019404009E-6	0.0
5	2.1928875058212025E-5	0.0	0.0	9.746166692538678E-6	0.0
6	2.1928875058212025E-5	2.4365416731346695E-6	0.0	1.2182708365673347E-5	0.0
7	2.1928875058212025E-5	2.4365416731346695E-6	0.0	1.2182708365673347E-5	0.0
8	2.1928875058212025E-5	2.4365416731346695E-6	0.0	1.2182708365673347E-5	0.0
9	2.6801958404481367E-5	2.4365416731346695E-6	0.0	1.2182708365673347E-5	0.0
10-11	3.045677091418337E-5	2.4365416731346695E-6	2.4365416731346695E-6	1.2182708365673347E-5	0.0
12-13	3.2893312587318034E-5	2.4365416731346695E-6	2.4365416731346695E-6	1.5837520875375354E-5	0.0
14-15	4.385775011642405E-5	4.873083346269339E-6	2.4365416731346695E-6	1.7055791711942687E-5	0.0
16-17	1.1086264612762746E-4	4.873083346269339E-6	2.4365416731346695E-6	1.7055791711942687E-5	0.0
18-19	2.0345122970674492E-4	4.873083346269339E-6	2.4365416731346695E-6	1.827406254851002E-5	1.2182708365673348E-6
20-21	2.61928229861977E-4	4.873083346269339E-6	2.4365416731346695E-6	2.3147145894779358E-5	2.4365416731346695E-6
22-23	3.740091468261718E-4	4.873083346269339E-6	2.4365416731346695E-6	2.4365416731346694E-5	2.4365416731346695E-6
24-25	5.043641263388766E-4	4.873083346269339E-6	2.4365416731346695E-6	2.4365416731346694E-5	2.4365416731346695E-6
26-27	6.444652725441201E-4	4.873083346269339E-6	2.4365416731346695E-6	2.6801958404481367E-5	2.4365416731346695E-6
28-29	8.36952064721759E-4	4.873083346269339E-6	2.4365416731346695E-6	2.8020229241048703E-5	2.4365416731346695E-6
30-31	9.575608775419252E-4	4.873083346269339E-6	2.4365416731346695E-6	2.9238500077616036E-5	2.4365416731346695E-6
32-33	9.9654554431208E-4	4.873083346269339E-6	2.4365416731346695E-6	2.9238500077616036E-5	2.4365416731346695E-6
34-35	0.0010574590861404465	4.873083346269339E-6	3.6548125097020045E-6	2.9238500077616036E-5	2.4365416731346695E-6
36-37	0.0012158342948942	4.873083346269339E-6	4.873083346269339E-6	2.9238500077616036E-5	2.4365416731346695E-6
38-39	0.0014533971080248303	6.0913541828366744E-6	4.873083346269339E-6	2.9238500077616036E-5	2.4365416731346695E-6
40-41	0.001822533171504733	7.309625019404009E-6	4.873083346269339E-6	2.9238500077616036E-5	2.4365416731346695E-6
42-43	0.0023744098604697355	7.309625019404009E-6	4.873083346269339E-6	3.16750417507507E-5	2.4365416731346695E-6
44-45	0.0032137984668646293	7.309625019404009E-6	4.873083346269339E-6	3.4111583423885374E-5	3.6548125097020045E-6
46-47	0.004389429824152107	7.309625019404009E-6	4.873083346269339E-6	3.4111583423885374E-5	4.873083346269339E-6
48-49	0.005950034765794863	7.309625019404009E-6	4.873083346269339E-6	3.4111583423885374E-5	6.0913541828366744E-6
50-51	0.00831469845957206	8.527895855971344E-6	4.873083346269339E-6	3.898466677015471E-5	7.309625019404009E-6
52-53	0.011641796114237452	9.746166692538678E-6	4.873083346269339E-6	4.263947927985672E-5	1.0964437529106013E-5
54-55	0.016077520230179117	1.2182708365673347E-5	4.873083346269339E-6	4.385775011642405E-5	1.2182708365673347E-5
56-57	0.022012935745935172	1.3400979202240682E-5	4.873083346269339E-6	4.385775011642405E-5	1.2182708365673347E-5
58-59	0.029646620807866092	1.827406254851002E-5	4.873083346269339E-6	4.629429178955872E-5	1.2182708365673347E-5
60-61	0.03967055325114212	1.9492333385077356E-5	4.873083346269339E-6	4.629429178955872E-5	1.2182708365673347E-5
62-63	0.052478234555974515	2.0710604221644692E-5	4.873083346269339E-6	4.7512562626126056E-5	1.7055791711942687E-5
64-65	0.06879088105761114	2.6801958404481367E-5	4.873083346269339E-6	5.116737513582806E-5	1.9492333385077356E-5
66-67	0.08970859132147227	2.9238500077616036E-5	4.873083346269339E-6	5.6040458482097406E-5	1.9492333385077356E-5
68-69	0.11549573011909305	3.167504175075071E-5	4.873083346269339E-6	5.9695270991799405E-5	1.9492333385077356E-5
70-71	0.14725483255756688	3.4111583423885374E-5	4.873083346269339E-6	6.091354182836674E-5	2.1928875058212025E-5
72-73	0.18657452380777761	3.4111583423885374E-5	4.873083346269339E-6	6.700489601120341E-5	2.1928875058212025E-5
74-75	0.23406515755884547	3.6548125097020047E-5	7.309625019404009E-6	7.065970852090541E-5	2.4365416731346694E-5
76-77	0.29061119843811833	3.6548125097020047E-5	1.2182708365673347E-5	7.065970852090541E-5	2.558368756791403E-5
78-79	0.35724939492751495	3.6548125097020047E-5	1.2182708365673347E-5	7.065970852090541E-5	2.6801958404481367E-5
80-81	0.4352601496762677	3.6548125097020047E-5	1.2182708365673347E-5	7.918760437687676E-5	2.8020229241048703E-5
82-83	0.5258605152501072	3.6548125097020047E-5	1.2182708365673347E-5	8.040587521344409E-5	3.16750417507507E-5
84-85	0.6297863272343203	3.898466677015471E-5	1.2182708365673347E-5	8.527895855971344E-5	3.16750417507507E-5
86-87	0.7489502708423176	4.1421208443289385E-5	1.2182708365673347E-5	9.015204190598278E-5	3.16750417507507E-5
88	0.848459851043974	4.1421208443289385E-5	1.2182708365673347E-5	9.989820859852146E-5	3.4111583423885374E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGATC	50280	0.0	44.588436	3
TATGGAT	51850	0.0	43.52883	2
ATATGGA	53015	0.0	42.703224	1
ATATCTC	31680	0.0	42.556263	1
GGATCCG	52930	0.0	41.490604	5
TATCTCG	32760	0.0	40.440636	2
TGGATCC	55335	0.0	40.21593	4
ATCTCGA	42500	0.0	38.309917	1
TCTCGAG	47580	0.0	34.041893	2
GATCCGG	73060	0.0	29.914919	6
CTCGAGG	54350	0.0	29.723473	3
TCGAGGG	59290	0.0	27.007784	4
ATCCGGC	93725	0.0	23.306437	7
CGGATCC	227190	0.0	21.831436	6
CCGGATC	229830	0.0	21.633472	5
GCGCCGG	232155	0.0	21.56061	2
GCCGGAT	232485	0.0	21.407558	4
CGCCGGA	232780	0.0	21.395512	3
GATCCTT	239955	0.0	20.771132	8
CGAGGGC	77960	0.0	20.689835	5
>>END_MODULE