FastQCFastQC Report
Wed 25 May 2016
SRR491003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25135705
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1115630.4438427328773949No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT781090.3107491912401104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA762220.30324194208994737No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT481930.19173124445882858No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT477420.18993698406310863No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396780.15785513077910485No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354490.14103045846535833No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343630.1367099112597001No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266520.10603243473775652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG212900.052.8340232
ATATCTC217150.052.78951
TATGGAT325800.050.004742
ATATGGA344700.047.6018261
ATGGATC349900.046.438553
GGATCCG360200.044.4974985
TGGATCC385900.041.9969864
ATCTCGA315300.035.5251273
TCTCGAG346950.031.9999144
GATCCGG508500.031.3896926
CTCGAGG396250.027.8644835
TCGAGGG432200.025.3752296
ATCCGGC636750.024.8445267
TCCGGCG756100.020.6293148
CGGATCC1539000.020.513716
CCGGATC1557500.020.276635
GCCGGAT1559700.020.2478664
CGCCGGA1578300.020.0269763
GCGCCGG1604150.019.8307882
CGAGGGC556550.019.7299697