##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491003_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25135705
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67185436016217	33.0	31.0	34.0	30.0	34.0
2	31.934941271788478	34.0	31.0	34.0	30.0	34.0
3	32.009524817386264	34.0	31.0	34.0	30.0	34.0
4	35.37265069748392	37.0	35.0	37.0	33.0	37.0
5	35.16790294921109	37.0	35.0	37.0	33.0	37.0
6	35.08382633389436	37.0	35.0	37.0	32.0	37.0
7	35.035294932049844	37.0	35.0	37.0	32.0	37.0
8	35.0051959950994	37.0	35.0	37.0	32.0	37.0
9	36.65587672993457	39.0	37.0	39.0	32.0	39.0
10-11	36.607496288645976	39.0	37.0	39.0	32.0	39.0
12-13	36.56593598627928	39.0	37.0	39.0	32.0	39.0
14-15	37.87646614646376	40.5	38.0	41.0	32.5	41.0
16-17	37.821713057183004	40.0	38.0	41.0	32.0	41.0
18-19	37.767646978670385	40.0	38.0	41.0	32.0	41.0
20-21	37.71346759519973	40.0	38.0	41.0	32.0	41.0
22-23	37.594098971960406	40.0	38.0	41.0	32.0	41.0
24-25	37.3536253508704	40.0	37.5	41.0	32.0	41.0
26-27	37.03290454753507	40.0	36.5	41.0	31.5	41.0
28-29	36.75948818622752	40.0	35.5	41.0	31.0	41.0
30-31	36.51811355599534	40.0	35.0	41.0	30.0	41.0
32-33	36.25273892258045	40.0	35.0	41.0	30.0	41.0
34-35	35.88020676165638	39.5	35.0	41.0	29.0	41.0
36-37	35.32580594019544	39.0	35.0	41.0	25.5	41.0
38-39	34.89704332939935	39.0	35.0	41.0	23.5	41.0
40-41	34.5499220531113	39.0	35.0	41.0	19.5	41.0
42-43	34.38402595033639	39.5	35.0	41.0	13.5	41.0
44-45	34.15734283164129	40.0	35.0	41.0	7.5	41.0
46-47	33.88710849765304	39.0	34.5	41.0	2.0	41.0
48-49	33.59215884336644	39.0	34.0	41.0	2.0	41.0
50-51	33.28857817992374	39.0	33.5	41.0	2.0	41.0
52-53	32.983564316178914	39.0	33.0	41.0	2.0	41.0
54-55	32.742674872258405	38.5	33.0	41.0	2.0	41.0
56-57	32.43286886124738	38.0	33.0	41.0	2.0	41.0
58-59	32.103434656000296	37.5	32.0	40.0	2.0	41.0
60-61	31.758142769419038	37.0	32.0	40.0	2.0	41.0
62-63	31.36879470856298	36.5	31.0	40.0	2.0	41.0
64-65	30.98056634974034	36.0	31.0	39.5	2.0	41.0
66-67	30.581157779342178	35.0	30.5	39.0	2.0	41.0
68-69	30.12803460257033	35.0	30.0	39.0	2.0	41.0
70-71	29.67279773931147	35.0	29.0	38.0	2.0	40.0
72-73	29.174761738331988	35.0	29.0	37.0	2.0	40.0
74-75	28.717734493621723	35.0	28.0	37.0	2.0	39.0
76-77	27.9906835515455	34.0	26.5	36.0	2.0	39.0
78-79	27.98397997987325	34.0	27.0	36.0	2.0	38.5
80-81	27.65477799409247	34.0	26.0	35.0	2.0	37.0
82-83	27.26021323054197	34.0	25.5	35.0	2.0	37.0
84-85	26.85926328702537	34.0	25.0	35.0	2.0	36.0
86-87	26.504706631463094	34.0	24.0	35.0	2.0	36.0
88-89	26.121146413040734	34.0	21.5	35.0	2.0	36.0
90-91	25.74689160300059	34.0	19.0	35.0	2.0	35.0
92-93	25.34841356150544	34.0	13.5	35.0	2.0	35.0
94-95	24.91568489923	33.0	3.5	35.0	2.0	35.0
96-97	24.45917261918852	33.0	2.0	35.0	2.0	35.0
98-99	24.016490307313838	33.0	2.0	35.0	2.0	35.0
100	23.10372177744766	32.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17787194968694564
1101	2	0.1910472102350731
1101	3	0.18071408110240839
1101	4	0.1874076782081886
1101	5	0.22135680610863773
1101	6	0.22926185313604464
1101	7	0.23484729950349958
1101	8	0.23712135761633846
1101	9	0.22605542359984554
1101	10-11	0.2736363041386696
1101	12-13	0.23882789149000416
1101	14-15	0.28757833812356637
1101	16-17	0.32444710179271397
1101	18-19	0.3218243294985186
1101	20-21	0.3020432932940764
1101	22-23	0.32582754841876493
1101	24-25	0.3060530742053871
1101	26-27	0.1854427315084095
1101	28-29	0.19596040187878572
1101	30-31	0.22585299815905557
1101	32-33	0.22005367166855194
1101	34-35	0.21016773444791426
1101	36-37	0.2722496495751727
1101	38-39	0.2549922036783201
1101	40-41	0.18159103603174032
1101	42-43	0.19134657504532981
1101	44-45	0.27537259162678396
1101	46-47	0.1404237371031556
1101	48-49	0.12744504960527792
1101	50-51	0.15107970317103536
1101	52-53	0.1330236017347204
1101	54-55	0.10099642945090181
1101	56-57	0.05827401964212697
1101	58-59	0.055993949980788216
1101	60-61	-0.018128265306422975
1101	62-63	-0.03235172538018105
1101	64-65	-0.033158302803556694
1101	66-67	-0.03440375764557402
1101	68-69	-0.01394388628303389
1101	70-71	-0.06863906682321641
1101	72-73	-0.04874592197748484
1101	74-75	-0.07542037209955765
1101	76-77	0.026825497586983715
1101	78-79	-0.025724916835891065
1101	80-81	-0.03814045635567709
1101	82-83	-0.06528320024258605
1101	84-85	-0.10536383486958201
1101	86-87	-0.07152723778622772
1101	88-89	-0.13124387013217742
1101	90-91	-0.1931255130003251
1101	92-93	-0.20802226336017426
1101	94-95	-0.2453212486045544
1101	96-97	-0.27148347089002556
1101	98-99	-0.33523911772309845
1101	100	-0.2980559786901189
1102	1	0.08641619213107177
1102	2	0.07275563263346996
1102	3	0.10329216710658784
1102	4	0.08981289008936955
1102	5	0.10045243054238995
1102	6	0.06279360466438533
1102	7	0.08308436598570523
1102	8	0.09954683713947077
1102	9	0.09903772880428363
1102	10-11	0.07808597852399402
1102	12-13	0.06610446947799886
1102	14-15	0.13069169488238686
1102	16-17	0.125125799775212
1102	18-19	0.14260993537363476
1102	20-21	0.174580846518964
1102	22-23	0.18989211565328645
1102	24-25	0.19929827866298666
1102	26-27	0.13311157908268711
1102	28-29	0.1280419693528856
1102	30-31	0.11707791742011864
1102	32-33	0.17041596073401877
1102	34-35	0.07777260156489518
1102	36-37	0.1616672542553914
1102	38-39	0.15283924057535359
1102	40-41	0.1095508142636632
1102	42-43	0.1232744992170467
1102	44-45	0.21817572908518912
1102	46-47	0.21343197232950928
1102	48-49	0.16162192327567482
1102	50-51	0.11673525786503802
1102	52-53	0.17867918631380775
1102	54-55	0.10913326376120835
1102	56-57	0.09804263296847893
1102	58-59	0.14400211614137248
1102	60-61	0.03830590832281189
1102	62-63	0.06701405923697124
1102	64-65	0.07506214813874479
1102	66-67	0.04376650529449577
1102	68-69	0.07611640570920386
1102	70-71	0.02509000866894695
1102	72-73	0.059698990764626814
1102	74-75	-0.021750495177546014
1102	76-77	0.07627208402650965
1102	78-79	0.04302472354885012
1102	80-81	0.038088142299017136
1102	82-83	0.016398458177075526
1102	84-85	0.008139875092869175
1102	86-87	0.04034977206605461
1102	88-89	-0.01322243594710315
1102	90-91	-0.1233880936287477
1102	92-93	-0.13542245101528394
1102	94-95	-0.1787203695934565
1102	96-97	-0.15473122128428685
1102	98-99	-0.19713136463699854
1102	100	-0.16932923129666833
1103	1	0.05576735778099007
1103	2	0.04732348339272008
1103	3	0.056334247415065875
1103	4	0.04929975238945161
1103	5	0.06913540571932941
1103	6	0.06003143818590928
1103	7	0.0789649761156781
1103	8	-0.018428531812993754
1103	9	0.002772373213140611
1103	10-11	0.055514281889081474
1103	12-13	0.027644571504389148
1103	14-15	0.07532179653453852
1103	16-17	0.1047608024938711
1103	18-19	0.10268975032779792
1103	20-21	0.10753418709962403
1103	22-23	0.08950093398626535
1103	24-25	0.09433497495923149
1103	26-27	0.031478000025202846
1103	28-29	0.0538743698667119
1103	30-31	0.06355922038451922
1103	32-33	0.08037952310363039
1103	34-35	0.10168148122858867
1103	36-37	0.11739128933727017
1103	38-39	0.023917964353202592
1103	40-41	0.04146476942680266
1103	42-43	0.05080771438555587
1103	44-45	0.07332303793857164
1103	46-47	-0.0042164496502152815
1103	48-49	0.009918299475835113
1103	50-51	0.016274298504391993
1103	52-53	0.06845039779441464
1103	54-55	-0.008754450398889446
1103	56-57	-0.008451078092981845
1103	58-59	0.07845626897058366
1103	60-61	0.055881684005512966
1103	62-63	0.0036700167872005807
1103	64-65	-0.01384279180505743
1103	66-67	-0.03045954365867587
1103	68-69	-0.042716972390305585
1103	70-71	-0.03746602126557619
1103	72-73	-0.08233404799862143
1103	74-75	-0.06405693022189496
1103	76-77	-0.028518194488544424
1103	78-79	-0.06713476739183832
1103	80-81	-0.012665507388412323
1103	82-83	0.002777352596581295
1103	84-85	-0.06541312117671438
1103	86-87	-0.010487917727409979
1103	88-89	-0.032642662603613815
1103	90-91	-0.09015668208128602
1103	92-93	-0.10061842927839493
1103	94-95	-0.18632709257484237
1103	96-97	-0.1400897095509137
1103	98-99	-0.12709516672004284
1103	100	-0.13381148363966133
1104	1	-0.04696977309436434
1104	2	-0.02449200858072942
1104	3	-0.024082272300290697
1104	4	-0.03058394695749911
1104	5	-0.0034391113050844524
1104	6	-0.049647343478440575
1104	7	-0.04264934369363971
1104	8	-0.02702853728394672
1104	9	-0.07517240512529355
1104	10-11	-0.07817185952172423
1104	12-13	-0.07311699925756443
1104	14-15	-0.12565454040292678
1104	16-17	-0.13043269723166873
1104	18-19	-0.007126008645556681
1104	20-21	-0.017363711017473804
1104	22-23	0.013243266211297566
1104	24-25	0.04054373630707886
1104	26-27	-0.015110882691260485
1104	28-29	1.903150252502428E-4
1104	30-31	-0.020328059041645474
1104	32-33	-0.03569819882183367
1104	34-35	-0.05924501015844186
1104	36-37	0.050077708392947784
1104	38-39	0.03705828017991308
1104	40-41	0.11115454449042517
1104	42-43	0.09831031306340776
1104	44-45	0.09303994910195712
1104	46-47	-0.03389313951750239
1104	48-49	-0.022394255160392618
1104	50-51	0.06936355893935087
1104	52-53	0.1244800626916529
1104	54-55	0.09554635089511976
1104	56-57	0.1081904846941697
1104	58-59	0.03433220765604972
1104	60-61	0.06750108764146034
1104	62-63	0.07065500929111934
1104	64-65	0.10735342340130316
1104	66-67	0.08180175227158415
1104	68-69	0.0772290165732592
1104	70-71	-0.02483256852319471
1104	72-73	0.10683299864243523
1104	74-75	0.17726120682924318
1104	76-77	0.18436434814617897
1104	78-79	0.11549775747860735
1104	80-81	0.10773679014001658
1104	82-83	0.11865384085638908
1104	84-85	0.1587438548543183
1104	86-87	0.16631253990177441
1104	88-89	0.12315757158905072
1104	90-91	0.07523385769206925
1104	92-93	0.09832888414090135
1104	94-95	0.09417524280702239
1104	96-97	0.14143285198684907
1104	98-99	0.1033808952359827
1104	100	0.18509145249175418
1105	1	-0.2039139079786203
1105	2	-0.20846308006809622
1105	3	-0.1973873452840067
1105	4	-0.1865644458261997
1105	5	-0.19622367931602724
1105	6	-0.2582193928582939
1105	7	-0.21296463383640685
1105	8	-0.2574341701550793
1105	9	-0.303463835778075
1105	10-11	-0.34362572139055914
1105	12-13	-0.3134386215044529
1105	14-15	-0.27487003593470405
1105	16-17	-0.2908199239654934
1105	18-19	-0.2663463576973939
1105	20-21	-0.2224420611095539
1105	22-23	-0.31340453518762956
1105	24-25	-0.3916167002735662
1105	26-27	-0.3130712999997485
1105	28-29	-0.3258232924953788
1105	30-31	-0.34536377312203825
1105	32-33	-0.3767329609378436
1105	34-35	-0.2983450296892656
1105	36-37	-0.2563417897240754
1105	38-39	-0.23202306884758173
1105	40-41	-0.17270003691825053
1105	42-43	-0.14098832631354696
1105	44-45	-0.16457212048255343
1105	46-47	-0.25630320271432794
1105	48-49	-0.2879890344030187
1105	50-51	-0.25896953039298864
1105	52-53	-0.1819625157502216
1105	54-55	-0.2181431322173566
1105	56-57	-0.20531269878834024
1105	58-59	-0.1947737923025059
1105	60-61	-0.12708989316721642
1105	62-63	-0.14902371669151648
1105	64-65	-0.24795221001310708
1105	66-67	-0.1745649727244718
1105	68-69	-0.10525753559141648
1105	70-71	-0.12628948151759545
1105	72-73	-0.04414561990703092
1105	74-75	-0.026216445661589205
1105	76-77	-0.06454435423913552
1105	78-79	-0.09639597191020854
1105	80-81	-0.2643022180929542
1105	82-83	-0.19373660701313256
1105	84-85	-0.07148959698772828
1105	86-87	-0.039571107553115326
1105	88-89	-0.06907980853621254
1105	90-91	-0.15227820086179378
1105	92-93	-0.1163054188294943
1105	94-95	2.809457142802785E-4
1105	96-97	0.030902910601287914
1105	98-99	0.041857003968317485
1105	100	0.1063907996613338
1106	1	-0.24531166500876367
1106	2	-0.21868725705940406
1106	3	-0.19538146093758257
1106	4	-0.20655044227503083
1106	5	-0.20995532027571073
1106	6	-0.22096208396082062
1106	7	-0.2367755642506424
1106	8	-0.2546792071632069
1106	9	-0.32150327892190944
1106	10-11	-0.2956999534385929
1106	12-13	-0.30579315938518903
1106	14-15	-0.3284464078019482
1106	16-17	-0.3226222075462033
1106	18-19	-0.30338365600480444
1106	20-21	-0.35751815156245925
1106	22-23	-0.341901205247936
1106	24-25	-0.33866935111741014
1106	26-27	-0.36876261190396775
1106	28-29	-0.3275177062019452
1106	30-31	-0.3058339611582426
1106	32-33	-0.33838189943543284
1106	34-35	-0.2944428050507497
1106	36-37	-0.23749997580348747
1106	38-39	-0.3417208112078143
1106	40-41	-0.2737794132542106
1106	42-43	-0.22778351417859
1106	44-45	-0.22018703540229723
1106	46-47	-0.22629331778920658
1106	48-49	-0.2726855228311891
1106	50-51	-0.23067205012196013
1106	52-53	-0.16510415958530444
1106	54-55	-0.23356059521344008
1106	56-57	-0.21170559531139332
1106	58-59	-0.22757145134910672
1106	60-61	-0.3145951851167297
1106	62-63	-0.27549122724455444
1106	64-65	-0.246745099055385
1106	66-67	-0.25511261562672516
1106	68-69	-0.23964436747194284
1106	70-71	-0.2809779388884195
1106	72-73	-0.2251773404723103
1106	74-75	-0.24357747909223804
1106	76-77	-0.22721156824130517
1106	78-79	-0.21538748264876162
1106	80-81	-0.24524836479819356
1106	82-83	-0.20974855543513016
1106	84-85	-0.20936115264226984
1106	86-87	-0.17962543148926358
1106	88-89	-0.2909101001394312
1106	90-91	-0.3300687907740816
1106	92-93	-0.30132801048141644
1106	94-95	-0.3378199864882383
1106	96-97	-0.40552978118025607
1106	98-99	-0.3725808200660765
1106	100	-0.3176311283931881
1107	1	-0.3644107105028205
1107	2	-0.33627239329775094
1107	3	-0.29803781165558973
1107	4	-0.32939015535854566
1107	5	-0.3416552066301435
1107	6	-0.38431035307758776
1107	7	-0.38618593345456986
1107	8	-0.38545751725132504
1107	9	-0.4439287655096109
1107	10-11	-0.43366178529869615
1107	12-13	-0.40770714072208847
1107	14-15	-0.433499613148733
1107	16-17	-0.4364770945985015
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2207	80-81	0.14542381909854996
2207	82-83	0.17443601707384815
2207	84-85	0.1663310240244691
2207	86-87	0.1388587302310853
2207	88-89	0.1697685901716035
2207	90-91	0.2771811744349151
2207	92-93	0.2752328169920908
2207	94-95	0.30536587992068576
2207	96-97	0.27908755619844783
2207	98-99	0.32947704291735036
2207	100	0.3349770420541134
2208	1	-0.36323713500905797
2208	2	-0.34349495492641324
2208	3	-0.3218928840761528
2208	4	-0.2906411894918648
2208	5	-0.3342733221515459
2208	6	-0.32730552269016755
2208	7	-0.3585706299657261
2208	8	-0.37817023611393097
2208	9	-0.40187129774645314
2208	10-11	-0.3937230941021923
2208	12-13	-0.4367937075215096
2208	14-15	-0.45276214953822347
2208	16-17	-0.4659423948007486
2208	18-19	-0.48069144824732746
2208	20-21	-0.44063694765982575
2208	22-23	-0.5135166405219636
2208	24-25	-1.4524877667180007
2208	26-27	-0.6537889583609271
2208	28-29	-0.5137078949126987
2208	30-31	-0.5037786180179324
2208	32-33	-0.505362344846688
2208	34-35	-0.5039158640024795
2208	36-37	-0.46030926667394567
2208	38-39	-0.4472619896490855
2208	40-41	-0.41287785785691966
2208	42-43	-0.4904020407638612
2208	44-45	-0.3864953936368565
2208	46-47	-0.34949301489428564
2208	48-49	-0.3848519680185305
2208	50-51	-0.39494175170820967
2208	52-53	-0.2886136475192913
2208	54-55	-0.2977203176366672
2208	56-57	-0.2649220928470868
2208	58-59	-0.28283359222920623
2208	60-61	-0.23936714862040986
2208	62-63	-0.16067030473190513
2208	64-65	-0.20158815143365416
2208	66-67	-0.20953039013884123
2208	68-69	-0.1286512207912196
2208	70-71	-0.08040995046722799
2208	72-73	-0.14362556633662749
2208	74-75	-0.08065334514023803
2208	76-77	0.01217299065680777
2208	78-79	0.04096223915751196
2208	80-81	0.06636211845513174
2208	82-83	0.13004601308516683
2208	84-85	0.12720483691639828
2208	86-87	0.17735964872107246
2208	88-89	0.251152269589614
2208	90-91	0.38754831031980075
2208	92-93	0.47881286877992935
2208	94-95	0.5625342018479458
2208	96-97	0.5552920423970988
2208	98-99	0.6750049215581235
2208	100	0.6128643125465736
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	361535.0
3	128243.0
4	70081.0
5	59896.0
6	63699.0
7	74689.0
8	83917.0
9	88449.0
10	92660.0
11	96553.0
12	127092.0
13	236186.0
14	445240.0
15	427694.0
16	369508.0
17	295388.0
18	259931.0
19	220406.0
20	192280.0
21	188985.0
22	195901.0
23	210799.0
24	233713.0
25	263390.0
26	294863.0
27	333424.0
28	378209.0
29	430315.0
30	497721.0
31	583428.0
32	697407.0
33	871864.0
34	1168127.0
35	1639775.0
36	2550179.0
37	4302524.0
38	5135386.0
39	1462855.0
40	3393.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.52878083162488	27.340924444228403	25.28794577622353	23.84234894792319
2	25.274050566978257	25.778667464408223	26.005053689582557	22.94222827903096
3	25.21213121526346	25.460783334952154	24.809843201938985	24.517242247845402
4	25.530566976339035	24.21339683927704	25.6428136787888	24.61322250559513
5	25.840437092004358	24.494285802302905	24.247869509215576	25.417407596477158
6	25.9267898699172	24.41509966797385	24.25372162604335	25.4043888360656
7	26.13004927771861	25.470865314445014	24.138595644353977	24.2604897634824
8	26.15342724936519	25.165655752489368	24.985910683590813	23.69500631455463
9	24.638905297815413	25.895743911761826	25.217857613658516	24.247493176764237
10-11	24.24386536767269	25.01945487419613	25.730552744140933	25.006127013990252
12-13	23.38689615095166	24.874788095773734	26.54326972194348	25.195046031331124
14-15	22.716704079763876	24.977183095166495	28.950065768684674	23.356047056384956
16-17	22.418890966610906	24.91996476292424	29.729296665802746	22.931847604662106
18-19	21.790116856429684	24.80406034008387	31.12637446121407	22.279448342272374
20-21	21.16361763289628	24.55481193796259	32.59968759584289	21.681882833298243
22-23	21.129803215223202	24.613854447396445	32.9086284760084	21.347713861371954
24-25	20.76932270550055	24.164445569967263	33.76533231319893	21.30089941133326
26-27	20.779696004029375	24.202475990773912	34.3218557164888	20.695972288707914
28-29	20.83480436035598	24.288762208114385	34.24018661841758	20.636246813112056
30-31	20.847526491795122	24.238865135335285	34.206934160753576	20.70667421211602
32-33	20.873986004558905	24.323532220697004	34.07184684112314	20.73063493362095
34-35	21.004582739318387	24.438805379310725	33.80454129840526	20.75207058296563
36-37	21.266094578602278	24.64375767255424	33.03243820117619	21.057709547667294
38-39	21.575472478836897	24.992606317650104	32.053546596654634	21.378374606858365
40-41	21.846004557898873	25.378375188822222	31.175808613375484	21.599811639903425
42-43	22.033474144741913	25.622589157952657	30.36108453981309	21.982852157492342
44-45	22.226972367394044	25.9007094388796	29.685188014416013	22.18713017931034
46-47	22.482715827704595	26.21567839023198	29.028361849511498	22.27324393255193
48-49	22.58560404134507	26.465592982086804	28.44960560850445	22.499197368063676
50-51	22.648684542878883	26.739787569035368	28.022567840081702	22.588960048004047
52-53	22.72779763706593	26.914504375054392	27.743591796515847	22.614106191363827
54-55	22.78062285782986	26.99396240693036	27.5367829501496	22.68863178509017
56-57	22.73777872536661	27.130831861960925	27.471185901390268	22.6602035112822
58-59	22.832141190358442	27.30430433869903	27.306739604741598	22.556814866200934
60-61	22.86715666737608	27.355815260095984	27.154782237667252	22.62224583486068
62-63	22.922665511416252	27.506525787496617	26.99775816776096	22.573050533326175
64-65	22.95891040291573	27.695633536724117	26.868147279021365	22.47730878133878
66-67	22.943153189301082	27.79884015431504	26.683473683155047	22.574532973228834
68-69	22.97213133710325	27.991064347884492	26.48824940498355	22.548554910028706
70-71	23.034558188948107	28.14207252361576	26.34363522611548	22.479734061320652
72-73	23.02706401625292	28.23957662009021	26.127368259088225	22.60599110456865
74-75	23.08499214166483	28.344187480136302	26.02117072938967	22.549649648809197
76-77	22.92395032248899	28.555974614198792	25.818734821237427	22.701340242074785
78-79	23.017114759842304	28.56450530999231	25.68441462640838	22.733965303757
80-81	23.030348322375836	28.658895502013866	25.513270606272453	22.79748556933785
82-83	23.04933398646761	28.73246961351929	25.382288270078956	22.835908129934143
84-85	23.032952302359995	28.748146338493815	25.20617570184489	23.01272565730131
86-87	23.0406269505199	28.878284295815433	25.071108158978557	23.009980594686112
88-89	23.142324596718645	28.93633905265075	24.88567813941867	23.03565821121194
90-91	23.135247539285476	28.948855761554654	24.753363537380118	23.16253316177975
92-93	23.139002733830257	29.04136743950993	24.643580157866136	23.176049668793677
94-95	23.171248960543846	29.106630391940396	24.53631368090709	23.18580696660867
96-97	23.144333657338386	29.201546415061713	24.335112406821857	23.319007520778044
98-99	23.192513489195214	29.24035186592825	24.24751447292224	23.31962017195429
100	23.559069256563475	29.085350453892904	23.687372399065463	23.668207890478154
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10507.0
1	6438.0
2	2177.5
3	2009.0
4	2086.5
5	2309.5
6	2738.5
7	3203.0
8	3979.5
9	5266.5
10	7731.0
11	11914.5
12	15066.5
13	21161.0
14	35485.5
15	46696.0
16	91910.5
17	96812.5
18	58062.0
19	59181.5
20	66365.5
21	73261.0
22	80135.0
23	92257.0
24	106380.0
25	122231.5
26	141453.0
27	166200.5
28	194763.0
29	224509.0
30	254996.0
31	289300.0
32	328991.0
33	369436.5
34	407460.0
35	456897.5
36	519227.0
37	587013.5
38	654138.5
39	707709.0
40	758184.5
41	817967.0
42	901131.0
43	974908.5
44	1034812.0
45	1084236.5
46	1079847.0
47	1024949.0
48	965161.5
49	950157.0
50	942709.0
51	904328.0
52	840784.0
53	766730.5
54	707733.0
55	657596.5
56	590171.5
57	523257.5
58	475744.5
59	426925.5
60	372413.5
61	321487.5
62	278485.5
63	242632.0
64	213571.5
65	184917.5
66	156620.5
67	131411.5
68	107499.0
69	86118.5
70	68360.0
71	54048.5
72	42671.0
73	33201.5
74	25341.5
75	19228.5
76	14389.0
77	10686.5
78	7736.0
79	5336.0
80	3556.5
81	2281.5
82	1477.5
83	952.0
84	613.5
85	391.5
86	253.0
87	169.5
88	98.5
89	68.0
90	54.0
91	37.5
92	17.5
93	8.0
94	7.5
95	6.5
96	4.5
97	4.5
98	4.0
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1491503818969868
2	0.018933226659049347
3	0.01674112582081943
4	0.0
5	0.0427399987388458
6	0.09000344330902993
7	0.09447516988284196
8	0.10444902977656684
9	0.07541463428218942
10-11	0.061241568517771836
12-13	0.057255207283821954
14-15	0.040362902094848746
16-17	0.039374268595211474
18-19	0.04302445465524042
20-21	0.03309435721019164
22-23	0.030812742272396977
24-25	0.03820859609865727
26-27	0.04060956316920492
28-29	0.06279314624356071
30-31	0.04579541333732235
32-33	0.043855941180086254
34-35	0.04287128608487409
36-37	0.03827225056945886
38-39	0.031164831064018295
40-41	0.028805637239934193
42-43	0.02902643868552722
44-45	0.031103165795429252
46-47	0.029889752445773854
48-49	0.029589382911678824
50-51	0.031127036221979848
52-53	0.023257752269132695
54-55	0.015589377739753073
56-57	0.020908504456111338
58-59	0.019814443239208925
60-61	0.00903893485382646
62-63	0.011595059696953
64-65	0.014425694445411417
66-67	0.01488321095429788
68-69	0.017771532566920243
70-71	0.017894863104098335
72-73	0.013836890590496666
74-75	0.02014664000870475
76-77	0.010031546757888827
78-79	0.012100317058940657
80-81	0.023396996424011184
82-83	0.01415516294450464
84-85	0.011994889341675516
86-87	0.002305485364345261
88-89	0.006892585666485186
90-91	0.013612110740478535
92-93	0.005458370871236753
94-95	0.007035808225788773
96-97	0.011404096284548216
98-99	0.006763287522669446
100	0.0027888615019948716
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.5135705E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.39703496764071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.00979138949934	34.28790679716101
2	10.824024369569102	9.39461128113571
3	3.6796278881028557	4.790548203837167
4	1.776313676862855	3.0834698699326304
5	1.0184715266688695	2.2099322228197678
6	0.6565404354133989	1.7095144939983196
7	0.4637978316844157	1.4089215503667172
8	0.3403130052859624	1.1814860312270246
9	0.2620056709925291	1.0233242339204658
>10	1.6168305747064187	13.391394437772854
>50	0.16859695196704233	5.116664430209843
>100	0.1693691212501409	14.464733384676967
>500	0.011945744405471406	3.630444279985419
>1k	0.002096075242648433	1.2445363541732712
>5k	5.514766978936029E-5	0.16322731064293908
>10k+	2.2059067915601116E-4	2.8992851181397254
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	111563	0.4438427328773949	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	78109	0.3107491912401104	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76222	0.30324194208994737	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48193	0.19173124445882858	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47742	0.18993698406310863	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39678	0.15785513077910485	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35449	0.14103045846535833	No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34363	0.1367099112597001	No Hit
CCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26652	0.10603243473775652	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.956808850199348E-6	0.0	0.0	1.1935213275299023E-5	0.0
2	7.956808850199348E-6	0.0	0.0	1.1935213275299023E-5	0.0
3	7.956808850199348E-6	7.956808850199348E-6	0.0	1.1935213275299023E-5	0.0
4	7.956808850199348E-6	7.956808850199348E-6	0.0	1.5913617700398696E-5	0.0
5	7.956808850199348E-6	7.956808850199348E-6	0.0	1.989202212549837E-5	0.0
6	7.956808850199348E-6	7.956808850199348E-6	0.0	1.989202212549837E-5	0.0
7	7.956808850199348E-6	7.956808850199348E-6	0.0	3.182723540079739E-5	0.0
8	1.1935213275299023E-5	7.956808850199348E-6	0.0	3.182723540079739E-5	3.978404425099674E-6
9	1.989202212549837E-5	7.956808850199348E-6	0.0	3.182723540079739E-5	7.956808850199348E-6
10-11	3.77948420384469E-5	7.956808850199348E-6	0.0	3.978404425099674E-5	7.956808850199348E-6
12-13	6.763287522669446E-5	7.956808850199348E-6	0.0	4.774085310119609E-5	7.956808850199348E-6
14-15	1.4322255930358826E-4	9.946011062749186E-6	0.0	5.37084597388456E-5	9.946011062749186E-6
16-17	3.2821836507072313E-4	1.1935213275299023E-5	0.0	5.5697661951395437E-5	1.1935213275299023E-5
18-19	6.663827412041953E-4	1.1935213275299023E-5	0.0	5.5697661951395437E-5	1.1935213275299023E-5
20-21	9.707306797243204E-4	1.1935213275299023E-5	0.0	5.768686416394527E-5	1.1935213275299023E-5
22-23	0.0013546467067464389	1.1935213275299023E-5	0.0	6.763287522669446E-5	1.1935213275299023E-5
24-25	0.0018002280023576025	1.1935213275299023E-5	0.0	7.161127965179413E-5	1.3924415487848859E-5
26-27	0.0023611830262966565	1.1935213275299023E-5	0.0	8.354649292709315E-5	1.5913617700398696E-5
28-29	0.0031548747091040413	1.1935213275299023E-5	0.0	9.548170620239218E-5	1.5913617700398696E-5
30-31	0.0037973870237576384	1.1935213275299023E-5	0.0	9.747090841494201E-5	1.989202212549837E-5
32-33	0.004207162679542905	1.1935213275299023E-5	0.0	9.946011062749185E-5	1.989202212549837E-5
34-35	0.00463086275081602	1.1935213275299023E-5	0.0	1.014493128400417E-4	1.989202212549837E-5
36-37	0.005193806976967624	1.1935213275299023E-5	0.0	1.0542771726514136E-4	1.989202212549837E-5
38-39	0.005796535247370225	1.1935213275299023E-5	0.0	1.1139532390279087E-4	1.989202212549837E-5
40-41	0.006630010974428607	1.3924415487848859E-5	0.0	1.1537372832789055E-4	1.989202212549837E-5
42-43	0.007704180169205519	1.989202212549837E-5	0.0	1.233305371780899E-4	1.989202212549837E-5
44-45	0.009239844277293992	1.989202212549837E-5	0.0	1.273089416031896E-4	1.989202212549837E-5
46-47	0.011292700960645424	1.989202212549837E-5	0.0	1.4123335709103843E-4	1.989202212549837E-5
48-49	0.01391248027457356	1.989202212549837E-5	0.0	1.4322255930358826E-4	1.989202212549837E-5
50-51	0.017294124035908284	1.989202212549837E-5	0.0	1.4322255930358826E-4	1.989202212549837E-5
52-53	0.021578865601740632	1.989202212549837E-5	0.0	1.4720096372868794E-4	2.188122433804821E-5
54-55	0.027160567010155474	2.7848830975697718E-5	0.0	1.511793681537876E-4	2.3870426550598045E-5
56-57	0.03416056959611835	3.182723540079739E-5	0.0	1.5913617700398695E-4	2.3870426550598045E-5
58-59	0.043603312499092425	3.5805639825897064E-5	0.0	1.6311458142908664E-4	2.3870426550598045E-5
60-61	0.05543110885491376	3.5805639825897064E-5	0.0	1.6908218806673615E-4	2.5859628763147882E-5
62-63	0.07066441939862042	3.5805639825897064E-5	0.0	1.7504979470438564E-4	2.7848830975697718E-5
64-65	0.08987613436742672	3.5805639825897064E-5	0.0	1.8101740134203516E-4	2.7848830975697718E-5
66-67	0.11365505761624749	3.978404425099674E-5	0.0	1.929526146173342E-4	2.7848830975697718E-5
68-69	0.14231150469024043	4.1773246463546574E-5	0.0	1.9494181682988402E-4	2.7848830975697718E-5
70-71	0.1778127965776174	4.376244867609641E-5	0.0	1.989202212549837E-4	2.7848830975697718E-5
72-73	0.22014898726731555	4.376244867609641E-5	0.0	2.0090942346753354E-4	3.182723540079739E-5
74-75	0.27033854829216053	4.376244867609641E-5	3.978404425099674E-6	2.0886623231773288E-4	3.182723540079739E-5
76-77	0.3297540291788116	4.376244867609641E-5	3.978404425099674E-6	2.148338389553824E-4	3.182723540079739E-5
78-79	0.40054575751903515	4.376244867609641E-5	3.978404425099674E-6	2.148338389553824E-4	3.5805639825897064E-5
80-81	0.48184047354152193	4.774085310119609E-5	3.978404425099674E-6	2.148338389553824E-4	3.5805639825897064E-5
82-83	0.5747541196875122	5.37084597388456E-5	3.978404425099674E-6	2.1881224338048206E-4	3.5805639825897064E-5
84-85	0.681041172308475	5.5697661951395437E-5	3.978404425099674E-6	2.1881224338048206E-4	3.5805639825897064E-5
86-87	0.8003336289950889	5.5697661951395437E-5	3.978404425099674E-6	2.2279064780558175E-4	3.5805639825897064E-5
88	0.8990318751751741	5.5697661951395437E-5	3.978404425099674E-6	2.307474566557811E-4	3.5805639825897064E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	21290	0.0	52.834023	2
ATATCTC	21715	0.0	52.7895	1
TATGGAT	32580	0.0	50.00474	2
ATATGGA	34470	0.0	47.601826	1
ATGGATC	34990	0.0	46.43855	3
GGATCCG	36020	0.0	44.497498	5
TGGATCC	38590	0.0	41.996986	4
ATCTCGA	31530	0.0	35.525127	3
TCTCGAG	34695	0.0	31.999914	4
GATCCGG	50850	0.0	31.389692	6
CTCGAGG	39625	0.0	27.864483	5
TCGAGGG	43220	0.0	25.375229	6
ATCCGGC	63675	0.0	24.844526	7
TCCGGCG	75610	0.0	20.629314	8
CGGATCC	153900	0.0	20.51371	6
CCGGATC	155750	0.0	20.27663	5
GCCGGAT	155970	0.0	20.247866	4
CGCCGGA	157830	0.0	20.026976	3
GCGCCGG	160415	0.0	19.830788	2
CGAGGGC	55655	0.0	19.729969	7
>>END_MODULE