FastQCFastQC Report
Wed 25 May 2016
SRR491002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37090751
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT639000.17228014606660297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA615480.16593894256818903No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT559990.15097833958659937No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT541050.14587194527282554No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT381320.1028073009360204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC261000.047.0105971
TATCTCG266850.045.4533732
ATCTCGA373900.032.941611
TCTCGAG423050.029.0601712
GATCCGG585600.028.1993711
TATGGAT336500.027.9357812
ATATGGA376550.025.3797871
ATGGATC385100.024.3607983
CTCGAGG507050.024.1525633
GGATCCG390900.023.3615725
ATCCGGC717650.023.0639422
TGGATCC418950.022.2577844
TCGAGGG573950.021.2389874
TCCGGCG815750.020.2783033
GCGCCGT939400.017.5111399
CGGCGCG956500.017.2353975
CCGGCGC984550.016.8111934
CGAGGGC777850.015.6655675
CGTCGAC906100.015.32252312-13
GCCGTCG947850.014.78218410-11
Produced by FastQC (version 0.11.5)