##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491002_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37090751
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6012557146659	33.0	31.0	34.0	30.0	34.0
2	31.86552054446134	34.0	31.0	34.0	30.0	34.0
3	31.937701504075772	34.0	31.0	34.0	30.0	34.0
4	35.29819935433499	37.0	35.0	37.0	33.0	37.0
5	35.07719967708392	37.0	35.0	37.0	32.0	37.0
6	34.998414456477306	37.0	35.0	37.0	32.0	37.0
7	34.949163633812645	37.0	35.0	37.0	32.0	37.0
8	34.9308735754636	37.0	35.0	37.0	32.0	37.0
9	36.58990067362076	39.0	37.0	39.0	32.0	39.0
10-11	36.53480007724837	39.0	37.0	39.0	32.0	39.0
12-13	36.47981543970355	39.0	37.0	39.0	32.0	39.0
14-15	37.76526788309032	40.0	38.0	41.0	32.0	41.0
16-17	37.68802849260183	40.0	38.0	41.0	32.0	41.0
18-19	37.62892991840472	40.0	38.0	41.0	32.0	41.0
20-21	37.582844440113924	40.0	38.0	41.0	32.0	41.0
22-23	37.46394278994243	40.0	38.0	41.0	32.0	41.0
24-25	37.32726638508883	40.0	38.0	41.0	31.5	41.0
26-27	37.12747177861133	40.0	37.5	41.0	31.0	41.0
28-29	36.86228444120746	40.0	37.0	41.0	30.5	41.0
30-31	36.653554278261986	40.0	36.0	41.0	30.0	41.0
32-33	36.45091950281622	40.0	36.0	41.0	30.0	41.0
34-35	36.223544880501336	40.0	35.0	41.0	30.0	41.0
36-37	35.94830300146794	40.0	35.0	41.0	28.5	41.0
38-39	35.756933177222535	40.0	35.0	41.0	27.5	41.0
40-41	35.53443961002569	40.0	35.0	41.0	26.0	41.0
42-43	35.4346694813486	40.0	35.0	41.0	25.0	41.0
44-45	35.226040866630065	40.0	35.0	41.0	23.5	41.0
46-47	34.97238648524534	40.0	35.0	41.0	22.5	41.0
48-49	34.69239556513698	40.0	35.0	41.0	19.0	41.0
50-51	34.407077953207256	39.0	35.0	41.0	16.0	41.0
52-53	34.11313723467072	39.0	34.0	41.0	11.5	41.0
54-55	33.8795741019102	39.0	34.0	41.0	8.5	41.0
56-57	33.571270975343694	38.5	34.0	41.0	6.0	41.0
58-59	33.238564190841004	38.0	33.0	41.0	2.0	41.0
60-61	32.888651567071264	37.5	33.0	40.0	2.0	41.0
62-63	32.49275006591266	37.0	32.5	40.0	2.0	41.0
64-65	32.098559045083775	36.0	32.0	40.0	2.0	41.0
66-67	31.687729657994794	36.0	31.5	39.0	2.0	41.0
68-69	31.2238255704232	35.0	31.0	39.0	2.0	41.0
70-71	30.762395334082072	35.0	31.0	38.5	2.0	40.5
72-73	30.25638343100683	35.0	30.0	37.5	2.0	40.0
74-75	29.79247418042304	35.0	30.0	37.0	2.0	39.0
76-77	29.04720755047532	34.0	29.0	36.0	2.0	39.0
78-79	29.060856748357562	35.0	29.0	36.0	2.0	39.0
80-81	28.73525811057317	35.0	29.0	35.5	2.0	37.0
82-83	28.33860925328797	34.0	29.0	35.0	2.0	37.0
84-85	27.933852188649404	34.0	27.5	35.0	2.0	36.5
86-87	27.57430210836119	34.0	27.0	35.0	2.0	36.0
88-89	27.184208362348876	34.0	26.0	35.0	2.0	36.0
90-91	26.798803507645342	34.0	25.0	35.0	2.0	35.5
92-93	26.391436398793864	34.0	24.0	35.0	2.0	35.0
94-95	25.94379323837363	34.0	21.5	35.0	2.0	35.0
96-97	25.469112811978384	33.5	18.0	35.0	2.0	35.0
98-99	25.008234128233205	33.0	2.0	35.0	2.0	35.0
100	24.073168267744162	32.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17176544990484643
1101	2	0.18514819136518668
1101	3	0.180840392225587
1101	4	0.18515439420140467
1101	5	0.2049402152706321
1101	6	0.2412462753978133
1101	7	0.24586459112517645
1101	8	0.2610380319854002
1101	9	0.25394833877667367
1101	10-11	0.2818108105837922
1101	12-13	0.2523136266592232
1101	14-15	0.2988243510637929
1101	16-17	0.3458636050358166
1101	18-19	0.3335529185710726
1101	20-21	0.31855471546581526
1101	22-23	0.33884995704384835
1101	24-25	0.3074765303722913
1101	26-27	0.19449221579356646
1101	28-29	0.17768558757108366
1101	30-31	0.22409170580296944
1101	32-33	0.21268296611332005
1101	34-35	0.21447560906345586
1101	36-37	0.24739732831304195
1101	38-39	0.24062084221522184
1101	40-41	0.1491560971786754
1101	42-43	0.15791387018737169
1101	44-45	0.22755489626621284
1101	46-47	0.07516399931510875
1101	48-49	0.05235980939919216
1101	50-51	0.07654133742862967
1101	52-53	0.07003885744265403
1101	54-55	0.009048742663786413
1101	56-57	-0.02225029503960485
1101	58-59	-0.035558292962043936
1101	60-61	-0.11751995016049221
1101	62-63	-0.1345034190519172
1101	64-65	-0.1199537236321504
1101	66-67	-0.1328519285293872
1101	68-69	-0.12495274209539886
1101	70-71	-0.17201668581354923
1101	72-73	-0.13378321017009043
1101	74-75	-0.18215175579343423
1101	76-77	-0.07575677031717021
1101	78-79	-0.1100659869619598
1101	80-81	-0.14158599949022133
1101	82-83	-0.16090387496635827
1101	84-85	-0.20966670830145873
1101	86-87	-0.19304625998447023
1101	88-89	-0.24626031308108765
1101	90-91	-0.3439179213033654
1101	92-93	-0.3745193510313243
1101	94-95	-0.4026297585665617
1101	96-97	-0.42467976647536787
1101	98-99	-0.46225587856013206
1101	100	-0.40246845552303157
1102	1	0.11672922202632208
1102	2	0.11320906455515001
1102	3	0.1341744630554622
1102	4	0.12460079184869954
1102	5	0.14942943678881448
1102	6	0.10739762348624993
1102	7	0.12742110241917715
1102	8	0.12250097901749513
1102	9	0.13152475128153895
1102	10-11	0.12527752565895867
1102	12-13	0.08792104487913832
1102	14-15	0.17199321236218168
1102	16-17	0.15258767324299782
1102	18-19	0.17490974531996528
1102	20-21	0.22124852069094914
1102	22-23	0.2264621367271289
1102	24-25	0.2438644811018449
1102	26-27	0.16896537911535603
1102	28-29	0.13734666213115077
1102	30-31	0.14294528996350664
1102	32-33	0.18370458287962066
1102	34-35	0.09565034932261796
1102	36-37	0.1791827884624908
1102	38-39	0.1884327691438017
1102	40-41	0.14199447865553338
1102	42-43	0.14834806953757607
1102	44-45	0.21998371863065103
1102	46-47	0.20049369673581907
1102	48-49	0.16995317285391565
1102	50-51	0.15249421606838354
1102	52-53	0.20248614195425318
1102	54-55	0.11834244460744259
1102	56-57	0.12047752520849997
1102	58-59	0.16671997004603156
1102	60-61	0.06946213089443631
1102	62-63	0.10239797686707419
1102	64-65	0.10674137803836459
1102	66-67	0.05632434469141856
1102	68-69	0.08157935954611517
1102	70-71	0.040271456568465425
1102	72-73	0.08848637888211641
1102	74-75	0.0015410369071062746
1102	76-77	0.10051019346062517
1102	78-79	0.06902410949345494
1102	80-81	0.061854965995721045
1102	82-83	0.054359975609081346
1102	84-85	0.030551461003511804
1102	86-87	0.04332602674744379
1102	88-89	0.01306356434213285
1102	90-91	-0.08186590648672265
1102	92-93	-0.10093742804214045
1102	94-95	-0.14161071610798004
1102	96-97	-0.0801435441521896
1102	98-99	-0.11361224721520813
1102	100	-0.08870923216935367
1103	1	0.04427448068135931
1103	2	0.03999294944657095
1103	3	0.043394621340485884
1103	4	0.03408846071203442
1103	5	0.06690790525382084
1103	6	0.04845463121615268
1103	7	0.08484869488104607
1103	8	-0.017096456775256286
1103	9	0.005017977898020831
1103	10-11	0.063767506627741
1103	12-13	0.03389737680012672
1103	14-15	0.09235973935327735
1103	16-17	0.10655562449552747
1103	18-19	0.11808748139782921
1103	20-21	0.11522493371153075
1103	22-23	0.10772460956867036
1103	24-25	0.1283852422047218
1103	26-27	0.04760427121433253
1103	28-29	0.07208504848638597
1103	30-31	0.0889448085038822
1103	32-33	0.08777724457341662
1103	34-35	0.1050492092295201
1103	36-37	0.13435510345345847
1103	38-39	0.0498772440540165
1103	40-41	0.04927547951949407
1103	42-43	0.05031752067357331
1103	44-45	0.09791982819621836
1103	46-47	0.04407356244780658
1103	48-49	0.057733216824907174
1103	50-51	0.06145811846764104
1103	52-53	0.11769071929279562
1103	54-55	0.06584515720151529
1103	56-57	0.06660521670801955
1103	58-59	0.1565494151946183
1103	60-61	0.11300969531362881
1103	62-63	0.0778184165172533
1103	64-65	0.06830038355959545
1103	66-67	0.035756403594611186
1103	68-69	0.025450229514593303
1103	70-71	0.046689218832487
1103	72-73	-0.01764936045406884
1103	74-75	0.02780797997920814
1103	76-77	0.0542954867317782
1103	78-79	0.017541997646002727
1103	80-81	0.059039545116473136
1103	82-83	0.07800974639426528
1103	84-85	-0.011819725929520075
1103	86-87	0.0554425920027839
1103	88-89	0.04062420829102109
1103	90-91	0.0011668331594805181
1103	92-93	-0.009023045880351077
1103	94-95	-0.0893741251639959
1103	96-97	-0.05723877118374432
1103	98-99	-0.04874530872566751
1103	100	-0.03785325811232099
1104	1	-0.06473244673312806
1104	2	-0.05045501493305338
1104	3	-0.046308910933756664
1104	4	-0.057106434452485644
1104	5	-0.020374989621615214
1104	6	-0.06491025106160464
1104	7	-0.04979599601230689
1104	8	-0.050138144884670055
1104	9	-0.10705728585947583
1104	10-11	-0.10734101781815752
1104	12-13	-0.09741599241804977
1104	14-15	-0.1686076714323903
1104	16-17	-0.1767263008812634
1104	18-19	-0.02719563192958674
1104	20-21	-0.06255454663870808
1104	22-23	-0.0246542013629778
1104	24-25	-0.02668742885010289
1104	26-27	-0.0775244968142701
1104	28-29	-0.05379740274182865
1104	30-31	-0.10072677545365849
1104	32-33	-0.08944947283552551
1104	34-35	-0.11487701299741104
1104	36-37	-0.007453803004672466
1104	38-39	-0.01872033129279771
1104	40-41	0.022219178183064514
1104	42-43	-0.005745564482467103
1104	44-45	0.017149536784586417
1104	46-47	-0.14058222364526785
1104	48-49	-0.12513443577936556
1104	50-51	-0.0353746007856941
1104	52-53	0.03637783574534126
1104	54-55	-0.01656885133630226
1104	56-57	-0.02774400363877305
1104	58-59	-0.08744407604876869
1104	60-61	-0.037126195949639396
1104	62-63	-0.05263386777863133
1104	64-65	0.008038382673220212
1104	66-67	-0.01885112368999131
1104	68-69	-0.03711101921046733
1104	70-71	-0.12016906545424533
1104	72-73	-0.0071465148189595595
1104	74-75	0.04058948181248567
1104	76-77	0.03680943195286446
1104	78-79	-0.017613696934503764
1104	80-81	0.011534977646082467
1104	82-83	0.0338322694909543
1104	84-85	0.04256443124517517
1104	86-87	0.03414007401072894
1104	88-89	0.012847944210832907
1104	90-91	-0.02366027403476778
1104	92-93	0.023873779304253162
1104	94-95	0.018748470072988965
1104	96-97	0.021983272579145563
1104	98-99	-0.01960844318175603
1104	100	0.05811413739316862
1105	1	-0.23412211259331883
1105	2	-0.22825834487049335
1105	3	-0.2023435123743873
1105	4	-0.19173617114747543
1105	5	-0.18726494577618524
1105	6	-0.27202073472210486
1105	7	-0.21155775153752643
1105	8	-0.2605557486219112
1105	9	-0.3340247276280408
1105	10-11	-0.354504524678255
1105	12-13	-0.3349248144303516
1105	14-15	-0.286165254031296
1105	16-17	-0.29242059938157894
1105	18-19	-0.2856502108589041
1105	20-21	-0.2513380937049732
1105	22-23	-0.3505669874563466
1105	24-25	-0.43412308823825896
1105	26-27	-0.3277116611025477
1105	28-29	-0.33754687180746856
1105	30-31	-0.34649727088282845
1105	32-33	-0.368944762919341
1105	34-35	-0.3041066225691935
1105	36-37	-0.23554545626770107
1105	38-39	-0.24757213235270115
1105	40-41	-0.20681397382853106
1105	42-43	-0.15352367527074762
1105	44-45	-0.17125731289814183
1105	46-47	-0.278765377254345
1105	48-49	-0.30708797593320725
1105	50-51	-0.279696314737059
1105	52-53	-0.18587279353381803
1105	54-55	-0.2532676326120722
1105	56-57	-0.24863104778075495
1105	58-59	-0.2217887992574532
1105	60-61	-0.14620096389383264
1105	62-63	-0.180682453053997
1105	64-65	-0.27549202152390606
1105	66-67	-0.23800695054839593
1105	68-69	-0.19182619564434233
1105	70-71	-0.16730233875664524
1105	72-73	-0.053007964372071115
1105	74-75	-0.045130622764020245
1105	76-77	-0.09702598714708444
1105	78-79	-0.1051865617332588
1105	80-81	-0.3155647262081267
1105	82-83	-0.25740486823184483
1105	84-85	-0.1135086924591846
1105	86-87	-0.07424317363376431
1105	88-89	-0.14650702228335888
1105	90-91	-0.2394305537135324
1105	92-93	-0.20193621427931063
1105	94-95	-0.0865582297271601
1105	96-97	-0.04936392669354106
1105	98-99	-0.03402006019532777
1105	100	0.03426737397876778
1106	1	-0.249434090311766
1106	2	-0.242398430918481
1106	3	-0.21955921255995747
1106	4	-0.2447809817222435
1106	5	-0.2364137701278537
1106	6	-0.24190561000194322
1106	7	-0.25696911624421404
1106	8	-0.2843251205326993
1106	9	-0.3347178365079557
1106	10-11	-0.3145247645713809
1106	12-13	-0.3269952967274037
1106	14-15	-0.34137314277288056
1106	16-17	-0.3362929172668814
1106	18-19	-0.3350852286177002
1106	20-21	-0.3786438125923013
1106	22-23	-0.34944694587586156
1106	24-25	-0.34239404638761073
1106	26-27	-0.3798190154092893
1106	28-29	-0.3362397238149697
1106	30-31	-0.3339850914467988
1106	32-33	-0.35113702544127534
1106	34-35	-0.33429258959859
1106	36-37	-0.24602200527786522
1106	38-39	-0.36278616453579104
1106	40-41	-0.32587620009869056
1106	42-43	-0.29233494903269275
1106	44-45	-0.2756744101699127
1106	46-47	-0.28590215306984845
1106	48-49	-0.3311399049596062
1106	50-51	-0.2834002457510252
1106	52-53	-0.21438952085100027
1106	54-55	-0.2502471069029042
1106	56-57	-0.22545487803979114
1106	58-59	-0.2651396825785639
1106	60-61	-0.32834087055633887
1106	62-63	-0.28882592455246936
1106	64-65	-0.2682726094181831
1106	66-67	-0.25697463676125665
1106	68-69	-0.2720729803329327
1106	70-71	-0.3047425118682554
1106	72-73	-0.24962630856966328
1106	74-75	-0.27206535936653964
1106	76-77	-0.2584679928944347
1106	78-79	-0.22225419976520655
1106	80-81	-0.2632152956742466
1106	82-83	-0.23098986931717036
1106	84-85	-0.20541735458851207
1106	86-87	-0.1970840553668083
1106	88-89	-0.31125799917465
1106	90-91	-0.3425156698731797
1106	92-93	-0.3266505045438315
1106	94-95	-0.3908994084123023
1106	96-97	-0.445452187491572
1106	98-99	-0.4104959473800811
1106	100	-0.3746893075908986
1107	1	-0.3781473868122198
1107	2	-0.32498744772659194
1107	3	-0.29234557782865167
1107	4	-0.33349254845277443
1107	5	-0.34421611913813877
1107	6	-0.39849744352873273
1107	7	-0.4042377068110241
1107	8	-0.39991763251639867
1107	9	-0.4619374509595957
1107	10-11	-0.45055529427305174
1107	12-13	-0.43776538964870326
1107	14-15	-0.4490936202945619
1107	16-17	-0.4399907686117075
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2207	82-83	0.15172486294797238
2207	84-85	0.1519241081215128
2207	86-87	0.09139755210085809
2207	88-89	0.14992425066925819
2207	90-91	0.26487154420989256
2207	92-93	0.24603978419019867
2207	94-95	0.29436825865548144
2207	96-97	0.2606721060092063
2207	98-99	0.30570201282415255
2207	100	0.3099744456024176
2208	1	-0.3666342963706697
2208	2	-0.3501935246775858
2208	3	-0.3281378719018946
2208	4	-0.29758934670621073
2208	5	-0.3305378759661437
2208	6	-0.33273734738396143
2208	7	-0.37952275883814224
2208	8	-0.3633909994710862
2208	9	-0.41276864866139107
2208	10-11	-0.39519476266145404
2208	12-13	-0.42711070424659425
2208	14-15	-0.4502314820316258
2208	16-17	-0.4675489812161615
2208	18-19	-0.48910821143741856
2208	20-21	-0.45500649481374467
2208	22-23	-0.5290131069639656
2208	24-25	-1.4523323131871493
2208	26-27	-0.6902662260106567
2208	28-29	-0.536006231118705
2208	30-31	-0.5080044659545919
2208	32-33	-0.5119356333638336
2208	34-35	-0.5176356693815194
2208	36-37	-0.4886569502697711
2208	38-39	-0.48851004601669246
2208	40-41	-0.4294824794253458
2208	42-43	-0.524594000018773
2208	44-45	-0.40787979689460485
2208	46-47	-0.3588910371960168
2208	48-49	-0.42943918473069687
2208	50-51	-0.45674131084545166
2208	52-53	-0.3364613207117273
2208	54-55	-0.3401297518822233
2208	56-57	-0.2865194890239451
2208	58-59	-0.30668385702661993
2208	60-61	-0.27507397295148905
2208	62-63	-0.1762789126467439
2208	64-65	-0.2230528152817186
2208	66-67	-0.18898666097688732
2208	68-69	-0.11575837846590176
2208	70-71	-0.08244909610009188
2208	72-73	-0.1398310662064226
2208	74-75	-0.10834482622111707
2208	76-77	-0.02326682726483753
2208	78-79	0.004450645758272032
2208	80-81	0.05404608935442212
2208	82-83	0.1159984850956981
2208	84-85	0.09260165922654551
2208	86-87	0.14201381796069512
2208	88-89	0.22390460596140827
2208	90-91	0.3584944957643259
2208	92-93	0.4425139942135985
2208	94-95	0.5390744102975091
2208	96-97	0.5612022499893605
2208	98-99	0.6796013589777772
2208	100	0.6185116273773339
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	599384.0
3	203766.0
4	106728.0
5	88079.0
6	88022.0
7	96787.0
8	108106.0
9	115786.0
10	123567.0
11	125551.0
12	136565.0
13	175091.0
14	268195.0
15	379388.0
16	458176.0
17	399458.0
18	352358.0
19	289106.0
20	255281.0
21	253899.0
22	266748.0
23	289112.0
24	322682.0
25	365408.0
26	416998.0
27	475655.0
28	549717.0
29	636332.0
30	747839.0
31	883964.0
32	1066680.0
33	1340760.0
34	1779084.0
35	2506165.0
36	3892061.0
37	6594353.0
38	7983254.0
39	2344661.0
40	5985.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.245082606805646	27.86484829993871	27.423588367276956	22.466480725978684
2	23.82251045572434	27.405164462030218	27.447951669305127	21.324373412940318
3	23.77283681041806	26.489088894078854	26.759372566950102	22.97870172855298
4	24.583009926113387	25.66794616803526	26.62772182747122	23.121322078380132
5	25.030665828178307	25.83562544277216	25.84906319424955	23.284645534799985
6	25.125749957982602	25.67778358399308	25.51284167271246	23.68362478531186
7	24.68719424165318	26.149423340854263	25.490956063634574	23.672426353857983
8	24.42075680120085	25.761936810303954	25.888030219018574	23.929276169476623
9	24.34549527291911	25.772813623399937	25.83914053575103	24.042550567929922
10-11	24.282209395314606	25.817504933109536	25.966912535063503	23.93337313651236
12-13	23.77783884103567	25.895505700756594	26.212699081634916	24.11395637657282
14-15	23.321274609573813	26.098966676385572	27.029123807062938	23.55063490697768
16-17	23.052352740428123	26.04024298537028	27.350057423975638	23.557346850225958
18-19	22.522956839993135	26.017033625509438	28.39520280105835	23.064806733439077
20-21	22.09850838082988	25.839156726253965	29.54505842532546	22.51727646759069
22-23	21.988931941601525	25.73200665143397	30.002256654653426	22.276804752311083
24-25	21.832062413150656	25.526576637650827	30.561402413830873	22.079958535367645
26-27	21.865285288533258	25.549210550838986	30.819324657081822	21.76617950354594
28-29	21.875287819192327	25.681132296282282	30.76169037590893	21.68188950861646
30-31	21.883953119075986	25.634380106723714	30.70984983629353	21.77181693790677
32-33	21.89038317913477	25.71441040569711	30.59961645214703	21.795589963021097
34-35	21.93776175304138	25.79363153298565	30.575246136513883	21.69336057745909
36-37	21.956970515421155	25.806855092434137	30.414971193475576	21.82120319866914
38-39	22.015485522282777	25.941031021760136	30.166452380690075	21.877031075267013
40-41	22.12138585469304	26.158117362120592	29.804429187301285	21.91606759588508
42-43	22.275590092291424	26.3645636887272	29.215989295004658	22.143856923976717
44-45	22.39699966864444	26.674868322471784	28.63937272948186	22.288759279401912
46-47	22.60369303412361	26.95015861984052	28.0891616277296	22.356986718306274
48-49	22.70352722308085	27.091229569682024	27.6203391240194	22.584904083217726
50-51	22.73517784066896	27.27860737963849	27.326152433225552	22.66006234646699
52-53	22.815759792735076	27.41823842342746	27.123120515546557	22.642881268290907
54-55	22.835842121446447	27.410445889537606	27.04583792110009	22.707874067915856
56-57	22.785902417429604	27.50082473215705	27.01618680875381	22.697086041659535
58-59	22.837641046829514	27.63077056580151	26.938922756436508	22.59266563093247
60-61	22.80180076420136	27.668297949578747	26.861346309177982	22.668554977041914
62-63	22.795751962621647	27.792493228868704	26.78843355657455	22.623321251935106
64-65	22.84973223688917	27.97436841959512	26.633355245714245	22.542544097801468
66-67	22.85648480108241	28.0560806907933	26.480297021305176	22.607137486819113
68-69	22.83669132582115	28.234795476780093	26.33632403461359	22.592189162785164
70-71	22.88466037557164	28.406839041864963	26.21032102576028	22.498179556803112
72-73	22.875112572422204	28.51908534075268	26.03093162608891	22.574870460736204
74-75	22.86611075887174	28.696632948765604	25.94821214728932	22.48904414507334
76-77	22.75504530580622	28.944231666367077	25.74491048061595	22.555812547210753
78-79	22.786236862636102	29.03955743268472	25.60614917767832	22.568056527000863
80-81	22.766241962519384	29.20949972316794	25.446294782685825	22.577963531626853
82-83	22.748421443009384	29.390776022719265	25.329241944395488	22.531560589875866
84-85	22.72603623871995	29.475313495932255	25.172565959046928	22.626084306300868
86-87	22.708924574697836	29.659311783390795	25.044548095294083	22.58721554661728
88-89	22.768575957417927	29.804295202823283	24.86024865507728	22.566880184681505
90-91	22.725252654655055	29.886561146982853	24.724101242066006	22.664084956296087
92-93	22.706666003032943	30.066385122036525	24.58958154730121	22.637367327629317
94-95	22.730506854180376	30.20125912244301	24.449734696132246	22.61849932724437
96-97	22.706101762147007	30.321158851060364	24.23129861178699	22.74144077500564
98-99	22.692977508246546	30.42338734683159	24.14173919186287	22.741895953059
100	23.00506602309099	30.362309517251372	23.63315301969444	22.999471439963195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8736.0
1	5577.0
2	2295.0
3	2214.5
4	2479.0
5	2989.0
6	3666.5
7	4502.5
8	5513.5
9	6793.0
10	8303.0
11	10371.5
12	13375.0
13	19263.5
14	31828.0
15	45270.0
16	73433.0
17	76525.5
18	58688.0
19	65022.0
20	75794.5
21	88145.0
22	103155.0
23	123525.0
24	147000.0
25	173438.5
26	204772.5
27	243452.0
28	289154.0
29	338753.0
30	391102.0
31	449033.0
32	513349.5
33	580591.5
34	646680.5
35	728120.5
36	823285.5
37	917530.5
38	1012911.0
39	1099748.0
40	1179431.5
41	1264324.5
42	1376988.0
43	1484737.0
44	1562697.5
45	1609569.5
46	1590401.0
47	1521082.0
48	1452089.5
49	1423632.5
50	1389026.5
51	1312306.5
52	1212475.0
53	1098703.0
54	1002511.5
55	925559.0
56	829717.5
57	735483.0
58	668728.5
59	606214.0
60	536885.0
61	467654.5
62	406413.5
63	353354.5
64	308533.0
65	264820.5
66	223680.0
67	186853.5
68	152968.5
69	123202.0
70	98218.5
71	77859.5
72	61617.0
73	49008.5
74	38353.5
75	29026.0
76	21641.5
77	16377.0
78	12229.0
79	8646.0
80	5719.5
81	3644.5
82	2282.0
83	1415.0
84	925.5
85	590.0
86	389.0
87	259.0
88	169.0
89	96.5
90	76.0
91	54.5
92	29.5
93	20.0
94	12.5
95	9.5
96	7.0
97	4.5
98	4.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1451871384324356
2	0.019511602771267695
3	0.017201053707432346
4	0.0
5	0.04339626339730894
6	0.09348691807291797
7	0.09804600613236437
8	0.1068918771690549
9	0.07784150825093836
10-11	0.0629806605964921
12-13	0.058569857482799415
14-15	0.04109110651331918
16-17	0.0398320325193739
18-19	0.04288805044686208
20-21	0.03330345077132571
22-23	0.031081872674942602
24-25	0.03864575295334408
26-27	0.04102370426524931
28-29	0.06257355101815006
30-31	0.04604651979141646
32-33	0.044129599856309196
34-35	0.042726285051494374
36-37	0.037963642202876936
38-39	0.030755645794284404
40-41	0.027686147417182252
42-43	0.028287375469965546
44-45	0.0303215753167144
46-47	0.028113477669945264
48-49	0.028895343747555827
50-51	0.030153069696539712
52-53	0.02096479523965422
54-55	0.01540545781885085
56-57	0.020219326376001392
58-59	0.01950351450149931
60-61	0.008828346452192354
62-63	0.011485343071106864
64-65	0.013944177080695942
66-67	0.014371507333458954
68-69	0.017299460989614365
70-71	0.017187573257818372
72-73	0.013967093845039698
74-75	0.01990523189999577
76-77	0.010049675187218506
78-79	0.01263522542317895
80-81	0.023461374508162425
82-83	0.014072241352028704
84-85	0.011928849863406648
86-87	0.002863247498008331
88-89	0.006652601884496757
90-91	0.01348179765893659
92-93	0.005443405554123183
94-95	0.007059711462838808
96-97	0.011483995026145466
98-99	0.006715959997682441
100	0.0035130051694019357
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.7090751E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.858846020107684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.57363754879881	32.47142949374813
2	11.642876943988643	9.747147864589651
3	4.022018579952967	5.0507116928479014
4	1.94992941855813	3.264871811260115
5	1.058707853591309	2.2158144511878657
6	0.7055104324140901	1.771911153360059
7	0.46476238760657385	1.3618092053132824
8	0.34636414951014743	1.1598722880984655
9	0.2581930017096385	0.9726894991829842
>10	1.563469019034817	13.30982259938864
>50	0.22591400851088425	6.458632061716349
>100	0.17116954584819447	14.905153742103494
>500	0.014657804055246096	4.220361773644727
>1k	0.0026150728049634748	1.4387822813436233
>5k	3.226548513753786E-5	0.07902877274866484
>10k+	1.4196813044869446E-4	1.5719613094660523
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	63900	0.17228014606660297	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	61548	0.16593894256818903	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55999	0.15097833958659937	No Hit
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	54105	0.14587194527282554	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38132	0.1028073009360204	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	8.088269768385115E-6	0.0	5.392179845590077E-6	0.0
2	0.0	8.088269768385115E-6	0.0	8.088269768385115E-6	0.0
3	0.0	1.0784359691180155E-5	0.0	1.3480449613975192E-5	0.0
4	0.0	1.3480449613975192E-5	0.0	1.887262945956527E-5	0.0
5	8.088269768385115E-6	1.3480449613975192E-5	0.0	1.887262945956527E-5	0.0
6	1.0784359691180155E-5	1.617653953677023E-5	0.0	1.887262945956527E-5	0.0
7	1.0784359691180155E-5	1.617653953677023E-5	0.0	2.156871938236031E-5	2.6960899227950387E-6
8	1.0784359691180155E-5	1.617653953677023E-5	0.0	2.426480930515535E-5	2.6960899227950387E-6
9	1.0784359691180155E-5	1.887262945956527E-5	0.0	2.426480930515535E-5	2.6960899227950387E-6
10-11	2.0220674420962788E-5	2.4264809305155345E-5	0.0	2.5612854266552867E-5	2.6960899227950387E-6
12-13	4.17893938033231E-5	3.50491689963355E-5	0.0	3.6397213957733025E-5	2.6960899227950387E-6
14-15	9.705923722062138E-5	4.0441348841925576E-5	0.0	4.5833528687515655E-5	2.6960899227950387E-6
16-17	2.1838328374639812E-4	4.448548372611814E-5	0.0	4.7181573648913176E-5	2.6960899227950387E-6
18-19	4.637274667207466E-4	4.5833528687515655E-5	0.0	5.2573753494503255E-5	5.392179845590077E-6
20-21	8.816214047539776E-4	5.2573753494503255E-5	0.0	5.392179845590077E-5	5.392179845590077E-6
22-23	0.0016715757521329239	5.392179845590077E-5	0.0	5.6617888378695806E-5	5.392179845590077E-6
24-25	0.0026408200793777404	5.392179845590077E-5	0.0	6.201006822428588E-5	8.088269768385115E-6
26-27	0.003553446518243861	5.796593334009333E-5	0.0	6.875029303127349E-5	8.088269768385115E-6
28-29	0.005277596023871288	6.335811318568341E-5	0.0	7.144638295406851E-5	8.088269768385115E-6
30-31	0.006629685120153	6.470615814708092E-5	0.0	7.549051783826108E-5	8.088269768385115E-6
32-33	0.0070192701139968824	6.605420310847844E-5	0.0	7.818660776105612E-5	8.088269768385115E-6
34-35	0.007411551197763561	6.875029303127349E-5	0.0	8.35787876066462E-5	8.088269768385115E-6
36-37	0.00792785241797881	7.68385627996586E-5	0.0	8.627487752944124E-5	8.088269768385115E-6
38-39	0.008507511751379744	7.818660776105612E-5	0.0	9.166705737503131E-5	8.088269768385115E-6
40-41	0.009217931446036238	8.223074264524867E-5	0.0	9.301510233642883E-5	8.088269768385115E-6
42-43	0.010135950064747948	8.627487752944124E-5	0.0	9.840728218201892E-5	8.088269768385115E-6
44-45	0.01133031790054615	9.031901241363379E-5	0.0	1.0110337210481395E-4	8.088269768385115E-6
46-47	0.012888657875921682	9.301510233642883E-5	0.0	1.0245141706621147E-4	1.2132404652577673E-5
48-49	0.015048225904080507	9.840728218201892E-5	2.6960899227950387E-6	1.0514750698900651E-4	1.3480449613975192E-5
50-51	0.017664781174153092	9.975532714341642E-5	2.6960899227950387E-6	1.0919164187319906E-4	1.3480449613975192E-5
52-53	0.021013324858264532	9.975532714341642E-5	4.0441348841925576E-6	1.1053968683459658E-4	1.3480449613975192E-5
54-55	0.02519226423859684	1.0514750698900651E-4	5.392179845590077E-6	1.1053968683459658E-4	1.3480449613975192E-5
56-57	0.030515693791155644	1.1053968683459658E-4	5.392179845590077E-6	1.1053968683459658E-4	1.3480449613975192E-5
58-59	0.037156163270999826	1.1727991164158418E-4	5.392179845590077E-6	1.1458382171878915E-4	1.3480449613975192E-5
60-61	0.045263305668844504	1.186279566029817E-4	5.392179845590077E-6	1.1997600156437922E-4	1.3480449613975192E-5
62-63	0.05522266184364938	1.186279566029817E-4	5.392179845590077E-6	1.253681814099693E-4	1.3480449613975192E-5
64-65	0.06773791126526395	1.2671622637136683E-4	5.392179845590077E-6	1.3480449613975193E-4	1.617653953677023E-5
66-67	0.08312180036473243	1.3884863102394448E-4	5.392179845590077E-6	1.40196675985342E-4	1.752458449816775E-5
68-69	0.10208205274678855	1.4424081086953456E-4	5.392179845590077E-6	1.4289276590813706E-4	1.887262945956527E-5
70-71	0.1252118081944472	1.4693690079232958E-4	5.392179845590077E-6	1.4558885583093207E-4	1.887262945956527E-5
72-73	0.1531352115248354	1.5098103567652216E-4	5.392179845590077E-6	1.5098103567652216E-4	1.887262945956527E-5
74-75	0.1870924641024389	1.590693054449073E-4	5.392179845590077E-6	1.5637321552211224E-4	1.887262945956527E-5
76-77	0.22744753806683504	1.6580953025189485E-4	5.392179845590077E-6	1.5772126048350975E-4	1.887262945956527E-5
78-79	0.2756293610771052	1.7389780002028E-4	5.392179845590077E-6	1.604173504063048E-4	1.887262945956527E-5
80-81	0.3329603652403803	1.7659388994307502E-4	5.392179845590077E-6	1.617653953677023E-4	1.887262945956527E-5
82-83	0.3996616299303295	1.7794193490447256E-4	5.392179845590077E-6	1.617653953677023E-4	1.887262945956527E-5
84-85	0.47726453422310056	1.8063802482726758E-4	5.392179845590077E-6	1.6850562017468992E-4	1.887262945956527E-5
86-87	0.5665361157017285	1.8063802482726758E-4	5.392179845590077E-6	1.7389780002028E-4	1.887262945956527E-5
88	0.6422032274299326	1.8063802482726758E-4	8.088269768385115E-6	1.7524584498167752E-4	1.887262945956527E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	26100	0.0	47.010597	1
TATCTCG	26685	0.0	45.453373	2
ATCTCGA	37390	0.0	32.94161	1
TCTCGAG	42305	0.0	29.060171	2
GATCCGG	58560	0.0	28.199371	1
TATGGAT	33650	0.0	27.935781	2
ATATGGA	37655	0.0	25.379787	1
ATGGATC	38510	0.0	24.360798	3
CTCGAGG	50705	0.0	24.152563	3
GGATCCG	39090	0.0	23.361572	5
ATCCGGC	71765	0.0	23.063942	2
TGGATCC	41895	0.0	22.257784	4
TCGAGGG	57395	0.0	21.238987	4
TCCGGCG	81575	0.0	20.278303	3
GCGCCGT	93940	0.0	17.511139	9
CGGCGCG	95650	0.0	17.235397	5
CCGGCGC	98455	0.0	16.811193	4
CGAGGGC	77785	0.0	15.665567	5
CGTCGAC	90610	0.0	15.322523	12-13
GCCGTCG	94785	0.0	14.782184	10-11
>>END_MODULE