FastQCFastQC Report
Wed 25 May 2016
SRR491001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39037531
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1738450.4453278564159193No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1072200.2746587636395345No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA765340.19605235792191877No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT738530.18918460801222292No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT569220.14581352493834715No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT512290.1312301231345804No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT481470.12333515662145743No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT475820.12188783148196539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC327750.051.952671
TATGGAT488600.051.9407272
TATCTCG352800.047.7691542
ATATGGA546300.046.8436551
ATGGATC550150.046.085793
GGATCCG576650.043.1199465
TGGATCC606050.041.4625784
ATCTCGA483950.036.4204671
TCTCGAG544850.032.200752
GATCCGG772800.032.0539326
CTCGAGG632350.027.4541993
ATCCGGC945850.026.0636547
ACGGTAC48200.025.68823266-67
TCGAGGG678850.025.4420854
CGTTAAT51200.024.5507576-77
TAACGGT51600.024.04088664-65
ATTCGTG50150.023.0788782
TTACGTC52400.023.00178170-71
AATTCGT50250.022.5903631
GAGTACG48850.022.12122394
Produced by FastQC (version 0.11.5)