##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491001_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39037531
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.577149922724367	33.0	31.0	34.0	30.0	34.0
2	31.84915767341946	34.0	31.0	34.0	30.0	34.0
3	31.925718176182812	34.0	31.0	34.0	30.0	34.0
4	35.28473573290278	37.0	35.0	37.0	33.0	37.0
5	35.067865844282004	37.0	35.0	37.0	32.0	37.0
6	34.981093937523866	37.0	35.0	37.0	32.0	37.0
7	34.92801409494878	37.0	35.0	37.0	32.0	37.0
8	34.89303508974479	37.0	35.0	37.0	32.0	37.0
9	36.53207378817067	39.0	37.0	39.0	32.0	39.0
10-11	36.486193350701406	39.0	37.0	39.0	32.0	39.0
12-13	36.44621907568899	39.0	37.0	39.0	32.0	39.0
14-15	37.749925001660586	40.0	38.0	41.0	32.0	41.0
16-17	37.687059153408036	40.0	38.0	41.0	32.0	41.0
18-19	37.63214772727302	40.0	38.0	41.0	32.0	41.0
20-21	37.57412822803778	40.0	38.0	41.0	32.0	41.0
22-23	37.44920597053128	40.0	38.0	41.0	32.0	41.0
24-25	37.19399716903203	40.0	37.5	41.0	31.0	41.0
26-27	36.89257846506737	40.0	36.5	41.0	31.0	41.0
28-29	36.62020831952718	40.0	35.5	41.0	30.5	41.0
30-31	36.37300904096624	40.0	35.0	41.0	30.0	41.0
32-33	36.09710009580267	40.0	35.0	41.0	30.0	41.0
34-35	35.69637197342219	39.5	35.0	41.0	28.0	41.0
36-37	35.11471025152692	39.0	35.0	41.0	24.5	41.0
38-39	34.69467132795873	39.0	35.0	41.0	21.5	41.0
40-41	34.366638479262434	39.0	35.0	41.0	16.5	41.0
42-43	34.21737993624647	39.0	35.0	41.0	10.5	41.0
44-45	33.99876722480221	39.0	35.0	41.0	6.0	41.0
46-47	33.72781340858877	39.0	34.0	41.0	2.0	41.0
48-49	33.427074153332086	39.0	34.0	41.0	2.0	41.0
50-51	33.11914749424086	39.0	33.0	41.0	2.0	41.0
52-53	32.81274265270516	39.0	33.0	41.0	2.0	41.0
54-55	32.57756789229319	38.0	33.0	41.0	2.0	41.0
56-57	32.26891111530593	38.0	32.5	41.0	2.0	41.0
58-59	31.941665060733477	37.5	32.0	40.0	2.0	41.0
60-61	31.597986921867573	37.0	31.5	40.0	2.0	41.0
62-63	31.21243550213255	36.0	31.0	40.0	2.0	41.0
64-65	30.828038125669373	36.0	30.5	39.5	2.0	41.0
66-67	30.428553114693653	35.0	30.0	39.0	2.0	41.0
68-69	29.977263124043372	35.0	29.5	39.0	2.0	41.0
70-71	29.526017744308675	35.0	29.0	37.5	2.0	40.0
72-73	29.03144083318179	35.0	28.5	37.0	2.0	39.5
74-75	28.577396249778197	35.0	27.5	36.5	2.0	39.0
76-77	27.850849647740276	34.0	26.5	36.0	2.0	39.0
78-79	27.85182340297085	34.0	26.5	36.0	2.0	37.5
80-81	27.53134466931323	34.0	26.0	35.0	2.0	37.0
82-83	27.135759802534643	34.0	25.5	35.0	2.0	37.0
84-85	26.73648369309012	34.0	24.5	35.0	2.0	36.0
86-87	26.378471463781867	34.0	23.5	35.0	2.0	36.0
88-89	25.992242017047644	34.0	20.0	35.0	2.0	36.0
90-91	25.61177916195571	34.0	18.5	35.0	2.0	35.0
92-93	25.210574165154043	33.5	9.5	35.0	2.0	35.0
94-95	24.770379817309653	33.0	2.0	35.0	2.0	35.0
96-97	24.30300814874793	33.0	2.0	35.0	2.0	35.0
98-99	23.85513723959643	33.0	2.0	35.0	2.0	35.0
100	22.94498107475086	31.0	2.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1610288958090038
1101	2	0.17264165465550363
1101	3	0.16908446656317722
1101	4	0.1701909484457076
1101	5	0.18711770191956845
1101	6	0.19669559236702128
1101	7	0.21538592985377392
1101	8	0.22580023628979262
1101	9	0.21383920987768334
1101	10-11	0.2540004739718995
1101	12-13	0.2354270853742122
1101	14-15	0.2801941709865545
1101	16-17	0.30635628288001726
1101	18-19	0.3049188148554549
1101	20-21	0.28622226925369887
1101	22-23	0.31893559847147657
1101	24-25	0.2814570905622702
1101	26-27	0.17806638469745195
1101	28-29	0.16286488568410107
1101	30-31	0.20441704605678268
1101	32-33	0.16577155002527633
1101	34-35	0.1591685569242003
1101	36-37	0.2049284588804099
1101	38-39	0.16197739064663352
1101	40-41	0.08163193913393485
1101	42-43	0.07358039207556288
1101	44-45	0.18175752374251175
1101	46-47	0.05227885900168161
1101	48-49	0.03251061176567305
1101	50-51	0.0749284454758552
1101	52-53	0.043408311947636946
1101	54-55	-0.007276038596963019
1101	56-57	-0.030197843106932964
1101	58-59	-0.025854648638091504
1101	60-61	-0.09733363412863127
1101	62-63	-0.11492761863817691
1101	64-65	-0.11450291522706735
1101	66-67	-0.10804047798740513
1101	68-69	-0.09923855490754718
1101	70-71	-0.1576146046898863
1101	72-73	-0.1566075331954977
1101	74-75	-0.18689310134014647
1101	76-77	-0.10512065512801527
1101	78-79	-0.1420608979589062
1101	80-81	-0.1569755710548435
1101	82-83	-0.18163053747532842
1101	84-85	-0.2099410209442958
1101	86-87	-0.19717357112980594
1101	88-89	-0.2461604876177006
1101	90-91	-0.33473014686203584
1101	92-93	-0.3468369012037229
1101	94-95	-0.38022620565368825
1101	96-97	-0.40081997369324895
1101	98-99	-0.4375965294005333
1101	100	-0.3979637845947437
1102	1	0.11669409799542763
1102	2	0.12388104061278327
1102	3	0.14261867744290413
1102	4	0.1295216967281121
1102	5	0.16979164593318075
1102	6	0.11514339969991028
1102	7	0.14227232450331684
1102	8	0.14031135947830364
1102	9	0.13558506325666286
1102	10-11	0.1312236410426948
1102	12-13	0.12458020884500343
1102	14-15	0.18598810672632027
1102	16-17	0.15855852616031996
1102	18-19	0.19384482687112836
1102	20-21	0.24419684317101087
1102	22-23	0.23543850769297592
1102	24-25	0.2610661509965482
1102	26-27	0.1875124946419291
1102	28-29	0.17687448467808764
1102	30-31	0.1991876278505984
1102	32-33	0.22118326648557485
1102	34-35	0.13485608244862135
1102	36-37	0.18121304144523975
1102	38-39	0.1993500188372863
1102	40-41	0.17067195187778594
1102	42-43	0.1922344165360883
1102	44-45	0.23891855685720742
1102	46-47	0.24520409623567474
1102	48-49	0.18894634860650683
1102	50-51	0.18149706147209343
1102	52-53	0.24112771201586014
1102	54-55	0.16168524408127638
1102	56-57	0.17314879367474134
1102	58-59	0.23259678722721233
1102	60-61	0.13609167049587967
1102	62-63	0.14626593451485803
1102	64-65	0.15548337703505055
1102	66-67	0.10399936946492261
1102	68-69	0.1519846461015355
1102	70-71	0.09629218462692535
1102	72-73	0.13803166654416188
1102	74-75	0.0694206703207314
1102	76-77	0.16076068046617564
1102	78-79	0.12362644239059506
1102	80-81	0.10941289882180882
1102	82-83	0.07397971459730002
1102	84-85	0.0684634374057893
1102	86-87	0.08470824121002707
1102	88-89	0.0663992942005791
1102	90-91	-0.03840191155483552
1102	92-93	-0.058023226867632616
1102	94-95	-0.09108769583519916
1102	96-97	-0.04685011332054856
1102	98-99	-0.1184057094207489
1102	100	-0.07545849439442165
1103	1	0.06974537073048026
1103	2	0.06767002661606725
1103	3	0.0680778925902743
1103	4	0.05175062626327076
1103	5	0.08719365438182791
1103	6	0.068086146043278
1103	7	0.08969134042845894
1103	8	0.005064010069091296
1103	9	0.049418627817416905
1103	10-11	0.0799301710984821
1103	12-13	0.0572230850271751
1103	14-15	0.11620927778403711
1103	16-17	0.1446629886654165
1103	18-19	0.1357042755457769
1103	20-21	0.1319091872259861
1103	22-23	0.12605840648717503
1103	24-25	0.12861860133676828
1103	26-27	0.0805125717160422
1103	28-29	0.07930376582218912
1103	30-31	0.10114557214760822
1103	32-33	0.10669168794582617
1103	34-35	0.1430218128387537
1103	36-37	0.16775911974556834
1103	38-39	0.061871689552241094
1103	40-41	0.049938512739977625
1103	42-43	0.0848963320783227
1103	44-45	0.1320797701630525
1103	46-47	0.056123064554839175
1103	48-49	0.08982025435063434
1103	50-51	0.08361541320750376
1103	52-53	0.143988649150387
1103	54-55	0.08350664996520152
1103	56-57	0.0932792318688982
1103	58-59	0.1618725238884231
1103	60-61	0.10980289360263384
1103	62-63	0.0888058619199299
1103	64-65	0.06152982241499316
1103	66-67	0.06817637153994482
1103	68-69	0.0667605316940616
1103	70-71	0.07586747601719779
1103	72-73	0.003716238020320617
1103	74-75	0.03474042040719283
1103	76-77	0.07233737136613172
1103	78-79	0.023679937829893305
1103	80-81	0.06345656390251264
1103	82-83	0.05041277892441798
1103	84-85	-0.007832205170878126
1103	86-87	0.044012782801342354
1103	88-89	0.037647932329555545
1103	90-91	-0.029645412750728184
1103	92-93	-0.02941728243539643
1103	94-95	-0.08229103096080337
1103	96-97	-0.05858172306250964
1103	98-99	-0.04813172444337965
1103	100	-0.051386772472707065
1104	1	-0.06486879881379082
1104	2	-0.056090655541545686
1104	3	-0.04046378038800924
1104	4	-0.051952881570265674
1104	5	-0.04018465895866541
1104	6	-0.06624762019463049
1104	7	-0.07256179864685208
1104	8	-0.06464247971872084
1104	9	-0.12119146691712501
1104	10-11	-0.12020496191367869
1104	12-13	-0.11083125014398831
1104	14-15	-0.17825405693690044
1104	16-17	-0.17031357129692992
1104	18-19	-0.04989561940360687
1104	20-21	-0.06395700941188665
1104	22-23	-0.026585203237424082
1104	24-25	-0.009625397256975532
1104	26-27	-0.0650428698203882
1104	28-29	-0.05817427097027661
1104	30-31	-0.09861897674957021
1104	32-33	-0.11002346426057841
1104	34-35	-0.12356937501406406
1104	36-37	0.0026239162902115254
1104	38-39	-0.00555029119987438
1104	40-41	0.04070615059902849
1104	42-43	0.018983754122352536
1104	44-45	0.03433225831849285
1104	46-47	-0.12839900104638247
1104	48-49	-0.1421372301900874
1104	50-51	-0.044526766820375485
1104	52-53	0.016398032045962907
1104	54-55	-0.0318436194349232
1104	56-57	-0.03684053218164962
1104	58-59	-0.10296280004165581
1104	60-61	-0.05911844257334309
1104	62-63	-0.06597450460350274
1104	64-65	-0.018993898112707797
1104	66-67	-0.04826362231763781
1104	68-69	-0.05507910949512862
1104	70-71	-0.14890199965041973
1104	72-73	-0.026255966123482466
1104	74-75	0.0534645376393712
1104	76-77	0.06650275103068282
1104	78-79	-0.015138091615462201
1104	80-81	-0.010649596700201158
1104	82-83	0.02009296602503241
1104	84-85	0.0336992486657941
1104	86-87	0.04615985169745329
1104	88-89	0.015571858036295794
1104	90-91	-0.024101283461568812
1104	92-93	0.011652246325013493
1104	94-95	0.020487132116652873
1104	96-97	0.04646069850697643
1104	98-99	0.001279188409128551
1104	100	0.07079851747299415
1105	1	-0.1948305799578769
1105	2	-0.19585413991252665
1105	3	-0.17374388969463084
1105	4	-0.1569806778950209
1105	5	-0.15864102897130294
1105	6	-0.2401522577734596
1105	7	-0.19063736317360025
1105	8	-0.22854283750188387
1105	9	-0.2973175102415482
1105	10-11	-0.31414685368767437
1105	12-13	-0.29476555129189563
1105	14-15	-0.2445654319805186
1105	16-17	-0.2774599685662622
1105	18-19	-0.25643232391973214
1105	20-21	-0.22165198068554304
1105	22-23	-0.33033091611188325
1105	24-25	-0.4001380440920954
1105	26-27	-0.3118863054094163
1105	28-29	-0.3367516894505016
1105	30-31	-0.33602237880773345
1105	32-33	-0.3594597237443651
1105	34-35	-0.30671060885440227
1105	36-37	-0.22556796582050964
1105	38-39	-0.2231765524542766
1105	40-41	-0.17748373762672287
1105	42-43	-0.15556715912600794
1105	44-45	-0.1538390711864821
1105	46-47	-0.23389709811841186
1105	48-49	-0.2561536524217587
1105	50-51	-0.23537857278022756
1105	52-53	-0.15490638428619263
1105	54-55	-0.21254985551806982
1105	56-57	-0.1858632391640782
1105	58-59	-0.19186861337724181
1105	60-61	-0.10357566381347638
1105	62-63	-0.13008983745824665
1105	64-65	-0.2361863539481952
1105	66-67	-0.19555843650474358
1105	68-69	-0.1388837254438613
1105	70-71	-0.14237145511230764
1105	72-73	-0.030803904650131386
1105	74-75	-0.009571238567662732
1105	76-77	-0.05777704589790744
1105	78-79	-0.07881795203634923
1105	80-81	-0.27801842530909227
1105	82-83	-0.2210287176014134
1105	84-85	-0.087669825389316
1105	86-87	-0.032244204035386304
1105	88-89	-0.09466048520003056
1105	90-91	-0.20399842762965648
1105	92-93	-0.17434961678675975
1105	94-95	-0.0598361989677656
1105	96-97	-0.022111004772956022
1105	98-99	-0.01564274202376481
1105	100	0.046917697278708204
1106	1	-0.2429094074049445
1106	2	-0.22629064589522585
1106	3	-0.19410069729719126
1106	4	-0.21465377030494892
1106	5	-0.2104773696045541
1106	6	-0.22066605235460202
1106	7	-0.23649855195180436
1106	8	-0.26190387639400825
1106	9	-0.31626364437878607
1106	10-11	-0.27931281488478277
1106	12-13	-0.2812690312968087
1106	14-15	-0.30752068832337187
1106	16-17	-0.29303920631415536
1106	18-19	-0.2867185846865894
1106	20-21	-0.3440424375669977
1106	22-23	-0.31288900994765356
1106	24-25	-0.30583017480277874
1106	26-27	-0.3315823915813283
1106	28-29	-0.2841472265517524
1106	30-31	-0.29485464608981005
1106	32-33	-0.33192838599140373
1106	34-35	-0.29206139136655906
1106	36-37	-0.21831369577213167
1106	38-39	-0.3310446351456946
1106	40-41	-0.2896333662092303
1106	42-43	-0.2621675001287329
1106	44-45	-0.2717196870592389
1106	46-47	-0.27905929495233295
1106	48-49	-0.3157202183203509
1106	50-51	-0.2665587723108871
1106	52-53	-0.19560755815643915
1106	54-55	-0.24931563294639858
1106	56-57	-0.2481826543596526
1106	58-59	-0.2620823568900654
1106	60-61	-0.3267906774701501
1106	62-63	-0.3011691640831984
1106	64-65	-0.2567409894321564
1106	66-67	-0.2546457083360032
1106	68-69	-0.26164204596184604
1106	70-71	-0.31010805416362786
1106	72-73	-0.2563183526777486
1106	74-75	-0.28322900250003613
1106	76-77	-0.29285428723614615
1106	78-79	-0.25586610726562853
1106	80-81	-0.2604838143022761
1106	82-83	-0.24001008278441205
1106	84-85	-0.2281532682751788
1106	86-87	-0.2273230264056103
1106	88-89	-0.3175443741435906
1106	90-91	-0.36899956195854955
1106	92-93	-0.34075726146965835
1106	94-95	-0.38961451402234815
1106	96-97	-0.45951651956208295
1106	98-99	-0.43039357458262373
1106	100	-0.39730350942028636
1107	1	-0.3536485004139607
1107	2	-0.3093370164375919
1107	3	-0.277845627704739
1107	4	-0.3179880601913396
1107	5	-0.3118656047837405
1107	6	-0.36332560967151295
1107	7	-0.3761357803319072
1107	8	-0.371437669220434
1107	9	-0.40894616294784214
1107	10-11	-0.41550833286127187
1107	12-13	-0.4023262033457229
1107	14-15	-0.4252567552621045
1107	16-17	-0.4131849331072601
1107	18-19	-0.41372067768780596
1107	20-21	-0.40525024744036386
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2207	78-79	0.08742696260885552
2207	80-81	0.11891981162194654
2207	82-83	0.14705109177965525
2207	84-85	0.16659890636323382
2207	86-87	0.12691367112926244
2207	88-89	0.17304348002332048
2207	90-91	0.2902578354327652
2207	92-93	0.28845999409546863
2207	94-95	0.33644850593898923
2207	96-97	0.2926901239198614
2207	98-99	0.34204891592941067
2207	100	0.32762595633735003
2208	1	-0.35040396878264346
2208	2	-0.3415545688166972
2208	3	-0.3218732872497938
2208	4	-0.2798960941633979
2208	5	-0.3055131686178143
2208	6	-0.29932197138958827
2208	7	-0.3309880322801817
2208	8	-0.34988227003249506
2208	9	-0.38771849143102344
2208	10-11	-0.37796338580869815
2208	12-13	-0.4082307892173276
2208	14-15	-0.4258636379408287
2208	16-17	-0.4406148034014308
2208	18-19	-0.4512142850787839
2208	20-21	-0.4153213228024413
2208	22-23	-0.47042070202965647
2208	24-25	-1.4021789980753354
2208	26-27	-0.6402480948391869
2208	28-29	-0.48339548976824886
2208	30-31	-0.4639080245009026
2208	32-33	-0.4807051693229738
2208	34-35	-0.47394306157445953
2208	36-37	-0.4085032960585764
2208	38-39	-0.39219341116967854
2208	40-41	-0.34419405852185747
2208	42-43	-0.4548762168282181
2208	44-45	-0.3362037230419972
2208	46-47	-0.2832332827108459
2208	48-49	-0.3402921159452603
2208	50-51	-0.3667858466659766
2208	52-53	-0.27860467028234837
2208	54-55	-0.29319947433796756
2208	56-57	-0.25010134931044803
2208	58-59	-0.2633818029945445
2208	60-61	-0.21604394557019901
2208	62-63	-0.1154409140441004
2208	64-65	-0.17349667448369743
2208	66-67	-0.1608133568958614
2208	68-69	-0.0929252498962434
2208	70-71	-0.05706336620880492
2208	72-73	-0.11055182835487543
2208	74-75	-0.0537884439335059
2208	76-77	0.024262559853841736
2208	78-79	0.04957502206577047
2208	80-81	0.06663716590625413
2208	82-83	0.14630946641718268
2208	84-85	0.14019190027317663
2208	86-87	0.1942598900357524
2208	88-89	0.27443182493557217
2208	90-91	0.41169266166485485
2208	92-93	0.4738488710763775
2208	94-95	0.5522578003201524
2208	96-97	0.5506249285890945
2208	98-99	0.6369334950308669
2208	100	0.5527487008186256
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	630808.0
3	211949.0
4	114303.0
5	97116.0
6	105295.0
7	121413.0
8	134991.0
9	143242.0
10	148928.0
11	159101.0
12	209420.0
13	390407.0
14	710163.0
15	632232.0
16	542120.0
17	442156.0
18	396295.0
19	335644.0
20	295637.0
21	292415.0
22	305189.0
23	332025.0
24	368844.0
25	415567.0
26	472052.0
27	533667.0
28	606417.0
29	695224.0
30	807610.0
31	942553.0
32	1114542.0
33	1379738.0
34	1823297.0
35	2554876.0
36	3958141.0
37	6695637.0
38	7801674.0
39	2112021.0
40	4822.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.980991602479822	27.33399128632992	26.28422173918977	24.400795372000488
2	24.678119433838553	25.896438392006218	26.139270001709203	23.286172172446033
3	24.919616265120773	25.49594416281293	25.01132564025032	24.573113931815982
4	24.95519504038306	24.082762816121747	26.05595369235826	24.90608845113693
5	25.543376545269712	24.33251905773173	24.600691575157512	25.523412821841042
6	25.69565106471245	24.468106572907	24.278400162864088	25.557842199516458
7	26.2675665272954	25.486518872762744	24.38469157263873	23.86122302730313
8	25.989846721217603	25.397160112035543	25.20017062489444	23.412822541852414
9	24.248971142512808	25.837897851107893	25.640856706114292	24.272274300265007
10-11	24.113612566116036	24.89099617177417	25.787385438387606	25.208005823722186
12-13	23.246757928677642	24.79918585898532	26.87032648981671	25.083729722520324
14-15	22.714966975981525	24.89911662595727	29.114240593136632	23.27167580492457
16-17	22.413931995427028	24.871488479011543	29.74838059165791	22.966198933903524
18-19	21.862225709480615	24.84234522287096	30.97326531052363	22.322163757124798
20-21	21.304684391040464	24.533686059331835	32.33288254731373	21.82874700231397
22-23	21.201088307523083	24.668733495675426	32.64876060599002	21.481417590811468
24-25	20.836759148733776	24.180611670135622	33.498103906634	21.484525274496608
26-27	20.834020827917783	24.181277717798764	34.10952128606883	20.87518016821462
28-29	20.897539992753106	24.34183389311875	33.965840187705666	20.79478592642248
30-31	20.923940844593375	24.27236564834224	33.920529822709696	20.88316368435469
32-33	20.97049129357736	24.36463636935954	33.79422225679712	20.870650080265975
34-35	21.10137132844221	24.45828201171739	33.45296598864407	20.987380671196334
36-37	21.36885202473621	24.76830033094324	32.64511848629071	21.217729158029837
38-39	21.718898375952815	25.068118609782413	31.647870166526165	21.565112847738604
40-41	21.97477899847164	25.48038180278272	30.79727769989833	21.747561498847315
42-43	22.06560884982918	25.661816688186352	30.078212661259883	22.19436180072459
44-45	22.32225784664114	25.883851707321675	29.428966405724815	22.364924040312374
46-47	22.553480546120316	26.2037571869973	28.834174753520024	22.408587513362352
48-49	22.64091077562746	26.527599440223682	28.223039464860626	22.608450319288227
50-51	22.719889417564975	26.7523448723069	27.81081028170242	22.716955428425706
52-53	22.73348558407936	26.92761161388635	27.59497627369079	22.7439265283435
54-55	22.837924891608015	27.055548285071612	27.376703125097677	22.729823698222695
56-57	22.74230219194487	27.221859936895783	27.313896053352916	22.721941817806435
58-59	22.81863808742044	27.49058772549857	27.094561515828257	22.59621267125273
60-61	22.876421409604657	27.536682011303416	26.92315486291699	22.663741716174936
62-63	22.92852960395931	27.7586414259224	26.694993713699773	22.617835256418527
64-65	22.924450862365596	27.97569302461297	26.621022799264082	22.478833313757356
66-67	22.916244520511956	28.112379570012614	26.424969210285294	22.546406699190143
68-69	22.97806606999187	28.304466790498644	26.177142961677006	22.540324177832478
70-71	22.97874420632404	28.451371143501973	26.084877544959213	22.485007105214773
72-73	22.9520078256441	28.576496733023145	25.865341927488483	22.606153513844273
74-75	23.063954960575796	28.698970169987785	25.750983532922355	22.48609133651406
76-77	22.823128303625786	29.00187243676145	25.53077438766389	22.64422487194887
78-79	22.926480705309565	29.001378150999575	25.443676977474265	22.628464166216595
80-81	22.91913079247106	29.167144737606055	25.22151189578004	22.692212574142843
82-83	22.973788263545558	29.17056257713653	25.13392172891637	22.721727430401543
84-85	22.920019457491385	29.168975943579323	24.94130894747161	22.96969565145768
86-87	22.954561415694833	29.326009700883265	24.831466593415655	22.88796229000625
88-89	23.022842658644972	29.366533215718675	24.65293099747336	22.957693128162994
90-91	23.089837456471702	29.35913601535593	24.48678202955986	23.064244498612513
92-93	23.066301450997695	29.45864947013136	24.352758679897654	23.12229039897329
94-95	23.146314705865738	29.463085651914994	24.287213296063513	23.103386346155748
96-97	23.08660548970447	29.576987512368667	24.07643968249206	23.2599673154348
98-99	23.167903666742312	29.53931151711408	24.00423247686268	23.288552339280923
100	23.524052817952793	29.43128448445995	23.38980384063868	23.654858856948575
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11199.0
1	6961.5
2	2563.5
3	2507.5
4	2745.0
5	3190.5
6	3893.0
7	4704.0
8	5888.5
9	8260.0
10	13370.5
11	20536.0
12	24070.5
13	31243.0
14	49817.5
15	63771.0
16	119654.0
17	138014.5
18	99738.0
19	98505.0
20	105992.0
21	117064.0
22	132612.5
23	152628.0
24	174265.0
25	197973.5
26	226704.0
27	262398.5
28	303715.0
29	349975.5
30	396904.5
31	448965.0
32	512619.0
33	575980.5
34	629589.0
35	697136.0
36	785718.0
37	886842.5
38	994500.0
39	1098103.0
40	1189608.0
41	1283844.5
42	1402361.0
43	1506640.5
44	1590114.0
45	1665349.0
46	1673136.0
47	1590958.0
48	1520491.5
49	1538737.5
50	1520022.0
51	1430359.0
52	1316654.0
53	1197020.0
54	1091625.5
55	989770.5
56	891661.0
57	799575.5
58	729014.0
59	657749.5
60	576369.0
61	497292.5
62	431719.0
63	376803.0
64	330471.5
65	285875.5
66	243765.5
67	205008.5
68	167230.0
69	133914.5
70	106107.0
71	83293.5
72	65640.5
73	51648.0
74	39587.5
75	29644.5
76	22022.0
77	16336.5
78	12003.0
79	8366.0
80	5476.0
81	3383.0
82	2150.0
83	1399.0
84	901.5
85	590.5
86	381.0
87	242.0
88	158.5
89	107.5
90	72.0
91	52.0
92	35.0
93	23.0
94	16.0
95	14.5
96	9.5
97	5.5
98	4.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14351061290223505
2	0.019478690903889387
3	0.016970847874574854
4	0.0
5	0.04291767325141541
6	0.09141459279276652
7	0.09698871580787218
8	0.10771429166460347
9	0.07670054748083324
10-11	0.06322377304035955
12-13	0.059326241713391153
14-15	0.04158818343301476
16-17	0.04006528998977932
18-19	0.04453278564159193
20-21	0.03451166007399392
22-23	0.03210244008515805
24-25	0.03891383397172326
26-27	0.041015657470755515
28-29	0.06394615479139805
30-31	0.0464655410712322
32-33	0.045014373475617604
34-35	0.04525516739263044
36-37	0.03836948602102935
38-39	0.031770708039911645
40-41	0.02829968934254577
42-43	0.028795366182354103
44-45	0.03199228967631175
46-47	0.03034259518103232
48-49	0.029677850271831997
50-51	0.03116231915384198
52-53	0.021758548203266236
54-55	0.015490221448687417
56-57	0.02122957007706251
58-59	0.020604530547795145
60-61	0.008914498204304979
62-63	0.011874470237372339
64-65	0.014997106246294111
66-67	0.014929222854795813
68-69	0.018507830323592954
70-71	0.018041612314057466
72-73	0.013690671164628725
74-75	0.020698030313443746
76-77	0.009998070830862741
78-79	0.01229073631731474
80-81	0.023446667259771115
82-83	0.014559066248323954
84-85	0.012167777721393292
86-87	0.002516808760267139
88-89	0.006952283944391873
90-91	0.013601013855102671
92-93	0.005503677986192313
94-95	0.00699198932432484
96-97	0.011342930473753579
98-99	0.006927948388949085
100	0.0028357326184383948
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.9037531E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.55073647791631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5080607056967	31.43008944591047
2	11.742223682766777	9.523116364492472
3	4.100829248467857	4.988749385866551
4	1.963475827045052	3.184815633730508
5	1.0925821726350797	2.215250588149719
6	0.6578777643894662	1.6006456715062776
7	0.462394164260898	1.3125296732709018
8	0.33647593517889557	1.0915477582879876
9	0.24662426068963494	0.900071586385769
>10	1.5113128148658335	12.163784983019285
>50	0.2035288222423676	5.6894096799566345
>100	0.1435284485530846	11.571483660971156
>500	0.021847128433975262	6.528749604605916
>1k	0.008985619167795545	4.798476934849918
>5k	5.7016263403079903E-5	0.1625680440447125
>10k+	1.9638934320039634E-4	2.838710984951726
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	173845	0.4453278564159193	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	107220	0.2746587636395345	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76534	0.19605235792191877	No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73853	0.18918460801222292	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56922	0.14581352493834715	No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51229	0.1312301231345804	No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48147	0.12333515662145743	No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47582	0.12188783148196539	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.5616374150301667E-6	0.0
2	0.0	0.0	2.5616374150301667E-6	5.123274830060333E-6	0.0
3	0.0	2.5616374150301667E-6	2.5616374150301667E-6	5.123274830060333E-6	0.0
4	0.0	2.5616374150301667E-6	2.5616374150301667E-6	5.123274830060333E-6	0.0
5	2.5616374150301667E-6	2.5616374150301667E-6	2.5616374150301667E-6	5.123274830060333E-6	0.0
6	2.5616374150301667E-6	5.123274830060333E-6	2.5616374150301667E-6	5.123274830060333E-6	0.0
7	2.5616374150301667E-6	5.123274830060333E-6	2.5616374150301667E-6	5.123274830060333E-6	0.0
8	5.123274830060333E-6	1.2808187075150833E-5	2.5616374150301667E-6	5.123274830060333E-6	0.0
9	7.6849122450905E-6	1.5369824490181E-5	2.5616374150301667E-6	5.123274830060333E-6	0.0
10-11	1.4089005782665918E-5	2.0493099320241334E-5	2.5616374150301667E-6	5.123274830060333E-6	0.0
12-13	4.610947347054301E-5	2.30547367352715E-5	2.5616374150301667E-6	5.123274830060333E-6	0.0
14-15	1.0374631530872175E-4	2.9458830272846916E-5	2.5616374150301667E-6	5.123274830060333E-6	0.0
16-17	2.4975964796544124E-4	3.4582105102907255E-5	2.5616374150301667E-6	5.123274830060333E-6	0.0
18-19	4.290742670175529E-4	3.5862923810422335E-5	2.5616374150301667E-6	5.123274830060333E-6	2.5616374150301667E-6
20-21	6.480942660026321E-4	3.7143742517937415E-5	5.123274830060333E-6	5.123274830060333E-6	2.5616374150301667E-6
22-23	9.631756680513427E-4	4.8671110885573166E-5	5.123274830060333E-6	5.123274830060333E-6	2.5616374150301667E-6
24-25	0.0012846611636376285	5.37943857156335E-5	5.123274830060333E-6	5.123274830060333E-6	2.5616374150301667E-6
26-27	0.0016996464248725156	6.1479297960724E-5	5.123274830060333E-6	5.123274830060333E-6	2.5616374150301667E-6
28-29	0.002260645018764122	6.276011666823909E-5	5.123274830060333E-6	1.2808187075150833E-5	2.5616374150301667E-6
30-31	0.00268587682965913	6.660257279078434E-5	5.123274830060333E-6	1.2808187075150833E-5	2.5616374150301667E-6
32-33	0.0029420405711621466	6.660257279078434E-5	5.123274830060333E-6	1.2808187075150833E-5	2.5616374150301667E-6
34-35	0.003271210978993523	6.660257279078434E-5	5.123274830060333E-6	1.5369824490181E-5	3.84245612254525E-6
36-37	0.0037207783453313175	7.428748503587483E-5	5.123274830060333E-6	1.5369824490181E-5	1.0246549660120667E-5
38-39	0.004315078225618316	7.6849122450905E-5	5.123274830060333E-6	1.5369824490181E-5	1.0246549660120667E-5
40-41	0.005177069215775968	7.6849122450905E-5	5.123274830060333E-6	1.5369824490181E-5	1.2808187075150833E-5
42-43	0.006361826520227419	8.069157857345025E-5	5.123274830060333E-6	1.6650643197696084E-5	1.2808187075150833E-5
44-45	0.008033294933534604	8.453403469599551E-5	5.123274830060333E-6	1.921228061272625E-5	1.2808187075150833E-5
46-47	0.010288816677468664	8.709567211102567E-5	5.123274830060333E-6	2.0493099320241334E-5	1.921228061272625E-5
48-49	0.0133743089438725	9.221894694108601E-5	5.123274830060333E-6	2.5616374150301666E-5	2.30547367352715E-5
50-51	0.01779569512221457	1.0118467789369158E-4	5.123274830060333E-6	2.8178011565331833E-5	2.30547367352715E-5
52-53	0.023856529246175942	1.0630795272375192E-4	5.123274830060333E-6	2.8178011565331833E-5	2.5616374150301666E-5
54-55	0.03195642675250133	1.1271204626132733E-4	5.123274830060333E-6	2.8178011565331833E-5	2.5616374150301666E-5
56-57	0.04230800354663823	1.152736836763575E-4	5.123274830060333E-6	3.0739648980362E-5	2.5616374150301666E-5
58-59	0.05605631155310514	1.1783532109138768E-4	6.404093537575417E-6	3.0739648980362E-5	2.689719285781675E-5
60-61	0.07340372012768942	1.1783532109138768E-4	7.6849122450905E-6	3.330128639539217E-5	3.0739648980362E-5
62-63	0.09505724119693942	1.1783532109138768E-4	8.965730952605584E-6	3.970537993296759E-5	3.202046768787708E-5
64-65	0.12264095288198426	1.1783532109138768E-4	1.2808187075150833E-5	4.739029217805808E-5	3.84245612254525E-5
66-67	0.1563892450062992	1.242394146289631E-4	1.2808187075150833E-5	5.251356700811841E-5	4.098619864048267E-5
68-69	0.19716282774133437	1.306435081665385E-4	1.2808187075150833E-5	5.5075204423148585E-5	4.226701734799775E-5
70-71	0.24642567686977948	1.3320514558156867E-4	1.2808187075150833E-5	5.6356023130663665E-5	4.3547836055512834E-5
72-73	0.3053932893450664	1.3576678299659885E-4	1.4089005782665918E-5	5.6356023130663665E-5	4.6109473470543E-5
74-75	0.3751082515951124	1.3576678299659885E-4	1.6650643197696084E-5	5.891766054569384E-5	4.6109473470543E-5
76-77	0.45635826712503924	1.3576678299659885E-4	1.7931461905211167E-5	7.6849122450905E-5	4.739029217805808E-5
78-79	0.5509710642304709	1.3704760170411392E-4	1.7931461905211167E-5	8.325321598848042E-5	4.8671110885573166E-5
80-81	0.6589761017416804	1.38328420411629E-4	1.7931461905211167E-5	8.965730952605584E-5	4.8671110885573166E-5
82-83	0.7821485943872833	1.396092391191441E-4	1.7931461905211167E-5	9.2218946941086E-5	4.8671110885573166E-5
84-85	0.9205564255587783	1.4345169524168934E-4	1.7931461905211167E-5	9.478058435611617E-5	4.8671110885573166E-5
86-87	1.076173336884446	1.460133326567195E-4	1.921228061272625E-5	9.478058435611617E-5	4.8671110885573166E-5
88	1.203949091964858	1.460133326567195E-4	2.0493099320241334E-5	9.734222177114633E-5	5.123274830060333E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCTC	32775	0.0	51.95267	1
TATGGAT	48860	0.0	51.940727	2
TATCTCG	35280	0.0	47.769154	2
ATATGGA	54630	0.0	46.843655	1
ATGGATC	55015	0.0	46.08579	3
GGATCCG	57665	0.0	43.119946	5
TGGATCC	60605	0.0	41.462578	4
ATCTCGA	48395	0.0	36.420467	1
TCTCGAG	54485	0.0	32.20075	2
GATCCGG	77280	0.0	32.053932	6
CTCGAGG	63235	0.0	27.454199	3
ATCCGGC	94585	0.0	26.063654	7
ACGGTAC	4820	0.0	25.688232	66-67
TCGAGGG	67885	0.0	25.442085	4
CGTTAAT	5120	0.0	24.55075	76-77
TAACGGT	5160	0.0	24.040886	64-65
ATTCGTG	5015	0.0	23.078878	2
TTACGTC	5240	0.0	23.001781	70-71
AATTCGT	5025	0.0	22.590363	1
GAGTACG	4885	0.0	22.121223	94
>>END_MODULE