FastQCFastQC Report
Wed 25 May 2016
SRR491000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR491000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29435822
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT532580.18092920931509915No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT475120.1614087760144765No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA323520.1099069018694297No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319900.10867710777704798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG205600.040.9218142
ATCTCGA244500.037.1409261
GGATCCG257500.035.2232785
ATGGATC263700.035.1819573
ATATCTC265500.032.7677571
GATCCGG403500.032.1256831
TCTCGAG290750.031.0562632
TGGATCC297300.030.999864
TATGGAT331700.028.3281142
CTCGAGG338150.026.4904063
ATATGGA359800.026.3243061
ATCCGGC502250.025.7595542
TCGAGGG405100.021.9960924
TCCGGCG709650.018.4139673
GCGCCGT806050.016.0202399
CCGGCGC816600.015.9906124
CGGCGCG816100.015.7978825
CGTCGAC790750.014.62711412-13
TCGACTT809550.014.31971114-15
GCCGTCG824700.014.20043110-11
Produced by FastQC (version 0.11.5)