##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR491000_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29435822
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77553716692539	33.0	31.0	34.0	30.0	34.0
2	32.01192706627999	34.0	31.0	34.0	30.0	34.0
3	32.09832163001937	34.0	31.0	34.0	30.0	34.0
4	35.45289722162337	37.0	35.0	37.0	33.0	37.0
5	35.22991136445926	37.0	35.0	37.0	33.0	37.0
6	35.15326947553902	37.0	35.0	37.0	32.0	37.0
7	34.955987911599685	37.0	35.0	37.0	32.0	37.0
8	34.87115854960667	37.0	35.0	37.0	32.0	37.0
9	36.454098513029464	39.0	37.0	39.0	32.0	39.0
10-11	36.331461781498746	39.0	37.0	39.0	32.0	39.0
12-13	36.16451123736242	39.0	37.0	39.0	32.0	39.0
14-15	37.37614344522127	40.0	38.0	41.0	32.0	41.0
16-17	37.39861071316439	40.0	38.0	41.0	32.0	41.0
18-19	37.321031157207024	40.0	38.0	41.0	32.0	41.0
20-21	37.19215787485058	40.0	38.0	41.0	31.0	41.0
22-23	37.01673945099954	40.0	38.0	41.0	31.0	41.0
24-25	36.813614360760845	40.0	37.5	41.0	30.0	41.0
26-27	36.50429867390827	40.0	37.0	41.0	30.0	41.0
28-29	36.20081251680351	40.0	36.0	41.0	29.5	41.0
30-31	35.89590134428724	40.0	35.5	41.0	27.5	41.0
32-33	35.60223254509421	40.0	35.0	41.0	26.5	41.0
34-35	35.296480152652094	39.0	35.0	41.0	25.0	41.0
36-37	34.912859865099065	39.0	35.0	41.0	23.5	41.0
38-39	34.49191758599437	39.0	35.0	40.0	19.5	41.0
40-41	34.00322245120248	39.0	34.0	40.0	12.0	41.0
42-43	33.44755235644515	38.0	33.5	40.0	5.0	41.0
44-45	32.92593690775817	38.0	33.0	40.0	2.0	41.0
46-47	32.40963027973196	38.0	32.5	40.0	2.0	41.0
48-49	31.85956823288305	38.0	31.5	40.0	2.0	41.0
50-51	31.353525952154488	37.5	31.0	40.0	2.0	41.0
52-53	30.861102757721525	37.0	30.0	40.0	2.0	41.0
54-55	30.295262197875772	36.5	28.5	39.5	2.0	40.0
56-57	29.68768944179646	36.0	27.5	39.0	2.0	40.0
58-59	29.083733571292825	35.5	26.0	39.0	2.0	40.0
60-61	28.50143792485224	35.0	25.5	38.5	2.0	40.0
62-63	27.87443153447524	34.0	23.0	38.0	2.0	40.0
64-65	27.198739260619256	34.0	21.0	38.0	2.0	40.0
66-67	26.498682914307608	33.5	18.0	37.0	2.0	39.0
68-69	25.777859524357773	33.0	10.5	36.0	2.0	39.0
70-71	25.04351969515239	32.0	4.5	36.0	2.0	38.5
72-73	24.16257115225116	31.0	2.0	35.0	2.0	38.0
74-75	23.29470484975755	30.5	2.0	34.5	2.0	37.0
76-77	22.547520449743175	29.5	2.0	34.0	2.0	36.0
78-79	22.458865154164883	30.0	2.0	34.0	2.0	36.0
80-81	21.884110370690514	29.0	2.0	34.0	2.0	35.5
82-83	21.22359377292063	29.0	2.0	34.0	2.0	35.0
84-85	20.83626715095641	29.0	2.0	34.0	2.0	35.0
86-87	20.20073140814617	28.0	2.0	33.5	2.0	35.0
88-89	19.41519781577698	26.0	2.0	33.0	2.0	35.0
90-91	18.68600068311325	25.5	2.0	32.5	2.0	34.0
92-93	17.87405179308395	24.5	2.0	32.0	2.0	34.0
94-95	16.937158999670537	19.0	2.0	32.0	2.0	34.0
96-97	16.00068389460977	8.5	2.0	31.0	2.0	34.0
98-99	15.04560010248737	2.0	2.0	31.0	2.0	34.0
100	14.427146896050669	2.0	2.0	31.0	2.0	34.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.05231281252625308
1101	2	-0.04617562050811941
1101	3	-0.07089038719545471
1101	4	-0.040831339232831
1101	5	0.014452685503840712
1101	6	-0.011331810844041001
1101	7	0.04340676672391908
1101	8	0.05157370970858466
1101	9	0.042492159309141186
1101	10-11	0.009429815129905705
1101	12-13	-0.014988913484053512
1101	14-15	-0.04201976908026239
1101	16-17	-0.05152134802806074
1101	18-19	-0.123909999864523
1101	20-21	-0.07729181838998755
1101	22-23	-0.09080286480381972
1101	24-25	-0.1373217244485403
1101	26-27	-0.18135374206934785
1101	28-29	-0.2084244764592711
1101	30-31	-0.2301068591899167
1101	32-33	-0.3050512943657182
1101	34-35	-0.3674658468947314
1101	36-37	-0.4283303321647196
1101	38-39	-0.4979185913101176
1101	40-41	-0.5639706094568098
1101	42-43	-0.5932651667111202
1101	44-45	-0.6751008688648454
1101	46-47	-0.7113060888253067
1101	48-49	-0.7772276425658191
1101	50-51	-0.8389363792655331
1101	52-53	-0.9464085014629688
1101	54-55	-0.9845436901078273
1101	56-57	-1.0622548009930988
1101	58-59	-1.2012337879649415
1101	60-61	-1.2923925793916204
1101	62-63	-1.345382414352997
1101	64-65	-1.4760071684859568
1101	66-67	-1.5925962756242171
1101	68-69	-1.7210907793708188
1101	70-71	-1.8185281411595753
1101	72-73	-1.8188481873047948
1101	74-75	-1.9090013416999163
1101	76-77	-1.70850932512678
1101	78-79	-1.7651753754023076
1101	80-81	-1.8360181257189296
1101	82-83	-1.836550423291019
1101	84-85	-1.865913890895122
1101	86-87	-2.030764351625784
1101	88-89	-1.9667871083196822
1101	90-91	-2.047900435013169
1101	92-93	-1.9789309286558492
1101	94-95	-1.8779135165240461
1101	96-97	-1.9883518316274618
1101	98-99	-2.0338896383447977
1101	100	-1.9083101919299725
1102	1	-0.025230833366606475
1102	2	-0.013417199916972322
1102	3	-0.013748700376631007
1102	4	-0.015502504270543227
1102	5	0.03599371697193021
1102	6	0.04090562896347194
1102	7	0.09286768019322977
1102	8	0.11715855092020888
1102	9	0.09950145156633283
1102	10-11	0.07479946375340774
1102	12-13	0.02996417876799029
1102	14-15	0.024267504247475813
1102	16-17	0.039574367176228975
1102	18-19	0.002623659225605479
1102	20-21	-0.08193724925145318
1102	22-23	-0.06932704543781654
1102	24-25	-0.10640284015037338
1102	26-27	-0.1006426556937896
1102	28-29	-0.10639820453003068
1102	30-31	-0.13468855652686074
1102	32-33	-0.1594526082550516
1102	34-35	-0.18038318408739684
1102	36-37	-0.23771233922064994
1102	38-39	-0.38548398483778357
1102	40-41	-0.40146873129221206
1102	42-43	-0.38137149726112796
1102	44-45	-0.4206161776823478
1102	46-47	-0.48776429668432186
1102	48-49	-0.5416529467936329
1102	50-51	-0.6042965222396219
1102	52-53	-0.7056025288623644
1102	54-55	-0.7107267445157532
1102	56-57	-0.8074031755727269
1102	58-59	-0.7724916904942916
1102	60-61	-0.9078193901578544
1102	62-63	-0.9291949741522139
1102	64-65	-1.0233015725084407
1102	66-67	-1.185806921954864
1102	68-69	-1.207705841181042
1102	70-71	-1.2648338242223858
1102	72-73	-1.3857296533980836
1102	74-75	-1.43971540834033
1102	76-77	-1.3082181740047858
1102	78-79	-1.2717648348315116
1102	80-81	-1.3152044274061012
1102	82-83	-1.305396827580939
1102	84-85	-1.2644156600034364
1102	86-87	-1.4616534773362417
1102	88-89	-1.3756313683197625
1102	90-91	-1.5242895457343693
1102	92-93	-1.4202274005562607
1102	94-95	-1.3203451597019384
1102	96-97	-1.4309670110471036
1102	98-99	-1.4985060738804936
1102	100	-1.3943511096077792
1103	1	0.020854592763701163
1103	2	0.036791811105636896
1103	3	0.03557317483888056
1103	4	0.046205047829651846
1103	5	0.08125316347096856
1103	6	0.08891010297235624
1103	7	0.11835963963548579
1103	8	0.1038378519392893
1103	9	0.12857805827926683
1103	10-11	0.1212256042056552
1103	12-13	0.10209350699621211
1103	14-15	0.1524241478037922
1103	16-17	0.18963085237745503
1103	18-19	0.17476286632766858
1103	20-21	0.14430133251320854
1103	22-23	0.16481679002235694
1103	24-25	0.11431556674382648
1103	26-27	0.09804780654179268
1103	28-29	0.0780402406516032
1103	30-31	0.07987694625574449
1103	32-33	0.09859665932928152
1103	34-35	0.1132964371560945
1103	36-37	0.10278970845226354
1103	38-39	0.055306309830164935
1103	40-41	0.08229130787111671
1103	42-43	0.0111793429846756
1103	44-45	0.02848431329580592
1103	46-47	-0.041273845047832936
1103	48-49	-0.22395712746168073
1103	50-51	-0.16505493851051511
1103	52-53	-0.2035323860916094
1103	54-55	-0.19344712463099967
1103	56-57	-0.1703171803452257
1103	58-59	-0.2456016286448488
1103	60-61	-0.29865874104756784
1103	62-63	-0.24091957800898456
1103	64-65	-0.33357389498998913
1103	66-67	-0.45742859422677995
1103	68-69	-0.4534003976073606
1103	70-71	-0.49363211375512606
1103	72-73	-0.5995904768942637
1103	74-75	-0.721615578698259
1103	76-77	-0.6402254787287731
1103	78-79	-0.6267686559088546
1103	80-81	-0.6415921824242261
1103	82-83	-0.6806355597836458
1103	84-85	-0.532868574282876
1103	86-87	-0.6743157817948031
1103	88-89	-0.6436700691265713
1103	90-91	-0.7088315783149
1103	92-93	-0.6007424777160573
1103	94-95	-0.5044966502883419
1103	96-97	-0.5753711726563733
1103	98-99	-0.6546274184561671
1103	100	-0.4995173562554278
1104	1	0.06347962414787744
1104	2	0.06508245739425433
1104	3	0.05238894995532917
1104	4	0.04294354192440153
1104	5	0.11024966093044952
1104	6	0.1238175086797284
1104	7	0.14428092487122512
1104	8	0.1530535295672948
1104	9	0.18698853132151783
1104	10-11	0.19795898474212237
1104	12-13	0.1881271936107609
1104	14-15	0.22505353127228744
1104	16-17	0.22693064484160175
1104	18-19	0.18303987670866917
1104	20-21	0.1788556277121245
1104	22-23	0.13254753516044815
1104	24-25	0.1392850188081951
1104	26-27	0.11625617838533486
1104	28-29	0.18576311860682893
1104	30-31	0.15160838674528776
1104	32-33	0.10352194878403509
1104	34-35	0.12242243005542264
1104	36-37	0.1377206816229375
1104	38-39	0.12407268159256546
1104	40-41	0.10140182899539951
1104	42-43	0.0949306757569488
1104	44-45	0.12798832568581986
1104	46-47	0.09721685548102244
1104	48-49	0.06491896834730682
1104	50-51	-0.020083261775738492
1104	52-53	0.03078805508334881
1104	54-55	-0.02673390522001995
1104	56-57	-0.03260942994802818
1104	58-59	0.024376029977950964
1104	60-61	-0.04717280372870647
1104	62-63	-0.1059418740475202
1104	64-65	-0.07129955978160751
1104	66-67	-0.16113147672152195
1104	68-69	-0.20426911763806288
1104	70-71	-0.22512856202415676
1104	72-73	-0.25218760261400774
1104	74-75	-0.3398969719964633
1104	76-77	-0.2748045063130924
1104	78-79	-0.305956000337833
1104	80-81	-0.25215707352267813
1104	82-83	-0.2864817641169992
1104	84-85	-0.16228893444909787
1104	86-87	-0.2665914190656906
1104	88-89	-0.28136005483336035
1104	90-91	-0.27375869865624125
1104	92-93	-0.19863911737056483
1104	94-95	-0.11883636664954977
1104	96-97	-0.15514604066143356
1104	98-99	-0.23348927039755374
1104	100	-0.13744992286594915
1105	1	0.05158661326509062
1105	2	0.06019947365020073
1105	3	0.03913993606467869
1105	4	0.031483051095293035
1105	5	0.0924065348786769
1105	6	0.07910674729318146
1105	7	0.1394684006324738
1105	8	0.13561699259491178
1105	9	0.14916569888806208
1105	10-11	0.1482118185607746
1105	12-13	0.15927207118374298
1105	14-15	0.16899552144669627
1105	16-17	0.19834598478504262
1105	18-19	0.17612542074581938
1105	20-21	0.18926279966250092
1105	22-23	0.18968494979114325
1105	24-25	0.2214299990302706
1105	26-27	0.2470793798303177
1105	28-29	0.22190196499131787
1105	30-31	0.2559868422919678
1105	32-33	0.26422549380487936
1105	34-35	0.3177360361136863
1105	36-37	0.280391591455853
1105	38-39	0.30340958529627216
1105	40-41	0.2926229056771774
1105	42-43	0.32878857058648947
1105	44-45	0.4139267743500312
1105	46-47	0.4035219822088507
1105	48-49	0.4298587598869048
1105	50-51	0.3721643162199868
1105	52-53	0.3769399395196018
1105	54-55	0.3813722421000172
1105	56-57	0.3536268624710992
1105	58-59	0.43031251828589134
1105	60-61	0.41216926044377544
1105	62-63	0.42914727893222704
1105	64-65	0.41491166761798226
1105	66-67	0.38839515553757664
1105	68-69	0.37071268808803026
1105	70-71	0.32211610258901047
1105	72-73	0.3020302564370496
1105	74-75	0.0859067330674641
1105	76-77	0.15717740851040674
1105	78-79	0.22269401211660877
1105	80-81	0.23485388030907828
1105	82-83	0.32504796151401294
1105	84-85	0.4106485870526413
1105	86-87	0.3207627341982757
1105	88-89	0.3177510338875358
1105	90-91	0.2289036700667495
1105	92-93	0.36629979051949135
1105	94-95	0.45845510037609927
1105	96-97	0.43224421226797816
1105	98-99	0.3678736755167922
1105	100	0.41358433860562727
1106	1	-0.05403924046460773
1106	2	-0.004277478645065003
1106	3	-0.029966548925855818
1106	4	-0.009740550338300125
1106	5	-0.005584887422642737
1106	6	-0.010533674609817467
1106	7	0.04526981544547937
1106	8	0.05377664434766416
1106	9	0.05431354081004969
1106	10-11	0.04566308922647977
1106	12-13	0.0403685851214064
1106	14-15	0.05986418022101958
1106	16-17	0.07566730269356725
1106	18-19	0.08096398109489655
1106	20-21	0.06130553728256416
1106	22-23	0.09451244594708896
1106	24-25	0.10006205111089628
1106	26-27	0.11722924612013941
1106	28-29	0.10172252732755283
1106	30-31	0.12572090035595096
1106	32-33	0.11667695468231898
1106	34-35	0.09046827760691656
1106	36-37	0.15364667331130732
1106	38-39	0.13432533035057048
1106	40-41	0.10595621237978037
1106	42-43	0.12465123694859415
1106	44-45	0.24058025944044914
1106	46-47	0.2641115091200774
1106	48-49	0.2381781412664168
1106	50-51	0.2477142718577241
1106	52-53	0.21921592874886286
1106	54-55	0.23989475730769882
1106	56-57	0.25619840301628827
1106	58-59	0.2635740208160904
1106	60-61	0.26889344092688106
1106	62-63	0.351375305379392
1106	64-65	0.3741865123335444
1106	66-67	0.3078568051459669
1106	68-69	0.3381386708133327
1106	70-71	0.32422145312876793
1106	72-73	0.31624733781112724
1106	74-75	0.14861166666929293
1106	76-77	0.22700273450383435
1106	78-79	0.271531860241236
1106	80-81	0.34065063973720555
1106	82-83	0.3084633181137164
1106	84-85	0.3504018705419334
1106	86-87	0.33124086590990487
1106	88-89	0.48464055623486146
1106	90-91	0.42984220185511646
1106	92-93	0.447701879470106
1106	94-95	0.4329795748569687
1106	96-97	0.2999414921820822
1106	98-99	0.27212602833463784
1106	100	0.33689175048373343
1107	1	-0.08270476245904135
1107	2	-0.10063197311903593
1107	3	-0.12683717552779683
1107	4	-0.12485631491241378
1107	5	-0.09908864809278839
1107	6	-0.050285571374367066
1107	7	-0.04710459754044649
1107	8	-0.05082021840583906
1107	9	-0.061290659393435476
1107	10-11	-0.012644407048171047
1107	12-13	0.002599678974156916
1107	14-15	-0.0071700420088731676
1107	16-17	-0.04300077677947911
1107	18-19	-0.019102961736678026
1107	20-21	-0.002057446116459971
1107	22-23	0.07544739338207762
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2207	82-83	1.1573559306931536
2207	84-85	1.0925028508440455
2207	86-87	1.173659209758938
2207	88-89	1.1392994344114733
2207	90-91	1.2491620278871949
2207	92-93	1.1298627935053922
2207	94-95	1.1720007011958877
2207	96-97	1.1012506808719813
2207	98-99	1.2434392951688888
2207	100	1.1225246381284872
2208	1	-0.3307265721526207
2208	2	-0.44157044290162517
2208	3	-0.3486851328243574
2208	4	-0.31122873825120934
2208	5	-0.3769990494567921
2208	6	-0.3377024235446626
2208	7	-0.48162501365491295
2208	8	-0.454417928788331
2208	9	-0.5408980951512419
2208	10-11	-0.4946011255104992
2208	12-13	-0.4569693888721176
2208	14-15	-0.46971882150759114
2208	16-17	-0.5624751431635246
2208	18-19	-0.5616757873830664
2208	20-21	-0.5446116533834271
2208	22-23	-0.5437007710062502
2208	24-25	-0.4692954354461776
2208	26-27	-0.45301773834480485
2208	28-29	-0.49581445065153673
2208	30-31	-0.40375699618666516
2208	32-33	-0.36467636738839815
2208	34-35	-0.37598972191389635
2208	36-37	-0.31976635116828334
2208	38-39	-0.24096342739496635
2208	40-41	-0.14666129909954861
2208	42-43	-0.0653508892182515
2208	44-45	0.0014203634173668434
2208	46-47	0.02347339649669067
2208	48-49	0.04724920122800569
2208	50-51	0.18159411297762773
2208	52-53	0.25983178170313437
2208	54-55	0.2575170442023662
2208	56-57	0.3541888846577379
2208	58-59	0.45075778811343525
2208	60-61	0.607313053226882
2208	62-63	0.7266241500313342
2208	64-65	0.8342083262179827
2208	66-67	0.9760872876577871
2208	68-69	0.8945129289284424
2208	70-71	0.9697769879597438
2208	72-73	1.11973732626533
2208	74-75	1.3348149017443554
2208	76-77	1.3557613723777706
2208	78-79	1.3386041997487474
2208	80-81	1.38706191464631
2208	82-83	1.3324843008408322
2208	84-85	1.2918884978124936
2208	86-87	1.3840906351109403
2208	88-89	1.4491148887343819
2208	90-91	1.5011653471645232
2208	92-93	1.4397296580229373
2208	94-95	1.3148129637874781
2208	96-97	1.175668005196112
2208	98-99	1.0438806739547832
2208	100	0.6147780763181938
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	396486.0
3	341822.0
4	141770.0
5	114750.0
6	108947.0
7	119769.0
8	132180.0
9	141707.0
10	164558.0
11	185716.0
12	220122.0
13	274023.0
14	401927.0
15	499606.0
16	568598.0
17	533016.0
18	494485.0
19	455592.0
20	448613.0
21	466220.0
22	490807.0
23	520671.0
24	550954.0
25	586860.0
26	627773.0
27	675868.0
28	740833.0
29	820993.0
30	932066.0
31	1073497.0
32	1270614.0
33	1567001.0
34	2066786.0
35	2985656.0
36	4058530.0
37	3365779.0
38	871142.0
39	20084.0
40	1.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.846770771161804	28.53230398592378	28.692179458790108	21.928745784124317
2	22.787980360835984	28.00989168138639	27.958297474765132	21.243830483012495
3	23.098013082362964	27.075887863421855	27.312363796164913	22.513735258050264
4	23.64099641588118	26.0390441797157	27.464266001724415	22.85569340267871
5	24.51576061165448	26.431827099528277	26.62113920048239	22.431273088334855
6	24.22146453096712	26.465586698303706	26.275052031980024	23.037896738749154
7	24.21935455889741	26.917554789625132	26.297562965792537	22.565527685684923
8	24.028680687093505	26.66539658021696	26.69354582919794	22.612376903491597
9	23.495725979704922	26.788737226640663	26.796710768574528	22.918826025079888
10-11	23.41475917314057	26.63465066627057	26.890437453942535	23.060152706646328
12-13	22.879174683304132	26.667189567053185	27.158389331382082	23.2952464182606
14-15	22.42155311568738	26.918275066764814	28.276786629048665	22.383385188499137
16-17	22.17708653652152	26.845048789087812	28.6327241668195	22.345140507571166
18-19	21.613584523770108	26.736248399624472	29.766004849763767	21.884162226841653
20-21	21.1369900499666	26.536536877011656	30.97154020872291	21.354932864298835
22-23	21.18023438723243	26.442911606372704	31.32571927285862	21.051134733536244
24-25	20.93462801691214	26.20486280163648	31.925013950682864	20.935495230768513
26-27	20.983814729544108	26.277930042193663	32.192759144226734	20.5454960840355
28-29	21.02533092472169	26.27061487922346	32.16237632646707	20.541677869587783
30-31	20.98752027741595	26.23314821328597	32.16236057990059	20.61697092939749
32-33	21.025524871432975	26.325181337381657	32.016892328227506	20.632401462957866
34-35	21.12124239704549	26.34514415829506	31.987259518798982	20.546353925860473
36-37	21.153487969248367	26.41668034083239	31.71651331932155	20.7133183705977
38-39	21.28561391955033	26.609091661190615	31.27413457009629	20.831159849162763
40-41	21.393667339282814	26.835495463707538	30.839332838509026	20.931504358500618
42-43	21.622038487103026	27.034546235257807	30.14491378433199	21.19850149330718
44-45	21.728745941457895	27.364586257630318	29.536014928782773	21.37065287212901
46-47	22.028777840670475	27.533441420244632	29.00041955463415	21.437361184450747
48-49	22.101868891021642	27.63398109078738	28.603205657276792	21.660944360914186
50-51	22.164953224935648	27.83243497161193	28.22711243612823	21.77549936732419
52-53	22.29607704504997	27.91287879390753	28.025238069493973	21.765806091548527
54-55	22.453561482724893	27.879990861331173	27.845884251501325	21.82056340444261
56-57	22.475685966500635	27.965673037513056	27.744833987025842	21.813807008960463
58-59	22.53928988688373	28.040413041737423	27.670470061865913	21.749827009512938
60-61	22.507238655832577	28.075161351447946	27.57784648088022	21.83975351183926
62-63	22.58611251526041	28.152785207246023	27.489998982853166	21.771103294640408
64-65	22.603695224734626	28.222973652970783	27.392080372672723	21.781250749621865
66-67	22.610313737613588	28.31763989769613	27.26225805469873	21.809788309991553
68-69	22.585576079097613	28.448896528607197	27.12727071082441	21.83825668147078
70-71	22.654451292404406	28.583399562798277	26.993478380851517	21.7686707639458
72-73	22.65790999800667	28.59493782908485	26.804658287178967	21.942493885729515
74-75	22.799938649178518	28.65940656808568	26.68514446827891	21.855510314456893
76-77	22.31448475745553	29.143063519834378	26.455146362491643	22.087305360218448
78-79	22.506866739691915	29.091024938640373	26.31640207279254	22.085706248875166
80-81	22.55916948543098	29.127754378915004	26.169816616807967	22.14325951884605
82-83	22.523367022784928	29.218967597336594	26.045787885447464	22.21187749443102
84-85	22.604745130891345	29.1971271716237	25.908298004985202	22.28982969249975
86-87	22.611062893964114	29.27566803765758	25.7589181040464	22.3543509643319
88-89	22.78190554039432	29.288199200408343	25.50465137480765	22.425243884389683
90-91	22.666754678731024	29.24754424173227	25.436720942264046	22.648980137272662
92-93	22.705431550945512	29.336548573559323	25.288043638522055	22.669976236973106
94-95	22.80232747220966	29.308879171846293	25.112048978623687	22.776744377320362
96-97	22.480297463846444	29.428174482738832	25.001017067833608	23.090510985581112
98-99	22.33859109477891	29.447476841001418	24.847321025069412	23.36661103915026
100	23.298325257965757	29.367025057142932	24.45821101320896	22.87643867168235
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3776.0
1	2704.5
2	1595.0
3	1761.5
4	2324.5
5	3317.5
6	4851.5
7	7051.0
8	10078.5
9	13884.0
10	18180.0
11	22343.5
12	25218.5
13	27459.5
14	31265.0
15	35232.0
16	48088.0
17	54496.5
18	48980.5
19	49508.0
20	62506.0
21	70645.5
22	73049.5
23	87073.5
24	106244.5
25	130290.5
26	157634.0
27	189039.5
28	225450.0
29	265163.0
30	307912.5
31	362798.5
32	431344.5
33	503258.0
34	575360.5
35	656201.5
36	753556.5
37	867635.0
38	971719.5
39	1036293.0
40	1072932.0
41	1130230.5
42	1247494.0
43	1356178.5
44	1436625.0
45	1471468.0
46	1414893.5
47	1329686.0
48	1258403.0
49	1201954.0
50	1129033.0
51	1036796.5
52	916681.0
53	775561.5
54	670636.0
55	588290.0
56	497402.5
57	416483.0
58	357873.0
59	308569.5
60	262189.5
61	218172.0
62	179456.0
63	149740.0
64	126518.5
65	106593.5
66	89355.5
67	74315.5
68	60985.0
69	50062.5
70	41229.0
71	34511.0
72	29465.0
73	25880.0
74	22503.0
75	18711.0
76	15719.0
77	13512.5
78	11583.0
79	10014.5
80	8599.5
81	7135.0
82	5651.0
83	4206.5
84	2933.0
85	1975.0
86	1321.0
87	882.5
88	604.5
89	438.5
90	320.5
91	220.0
92	148.5
93	104.5
94	76.0
95	46.5
96	26.5
97	15.5
98	7.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09489797838837319
2	0.014346465337370228
3	0.005221529060747819
4	0.007093397969317792
5	0.12126041528583778
6	0.05250065719245075
7	0.6781872780722753
8	0.711598949062812
9	0.6850055011203696
10-11	0.6699744957011902
12-13	0.6626993463950148
14-15	0.6234342631912912
16-17	0.6318576732798561
18-19	0.6758228120825027
20-21	0.7205540242769507
22-23	0.6816677312425656
24-25	0.6939775624407567
26-27	0.6984788805965738
28-29	0.7317359780202504
30-31	0.76667639857314
32-33	0.6784590557722492
34-35	0.6950273038069057
36-37	0.6857732731227958
38-39	0.7085108749468589
40-41	0.71557030070368
42-43	0.7199680036113821
44-45	0.7211417435531442
46-47	0.736323925317934
48-49	0.6520235786179166
50-51	0.7226552056198736
52-53	0.7464391515888362
54-55	0.7227503278148645
56-57	0.7226891778323703
58-59	0.7289145857723967
60-61	0.7037564638079412
62-63	0.7199374286201351
64-65	0.722775806974237
66-67	0.7085941068674759
68-69	0.7526186970419919
70-71	0.7148127203650029
72-73	0.3839777261868209
74-75	0.8921731487573202
76-77	0.7104948521566681
78-79	0.7485980856930036
80-81	0.4280260969100846
82-83	3.397221249673272E-6
84-85	0.001022563596151655
86-87	0.004322964040209239
88-89	0.003133936602823594
90-91	0.0015746120492235616
92-93	6.047053824418424E-4
94-95	0.0012518760305046009
96-97	0.002430711804141226
98-99	0.0015270509517281358
100	0.0014370245886117941
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.9435822E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.49749879185272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.97025881773402	34.43058215048625
2	11.037669270493659	9.16071334434361
3	2.8734537789554095	3.5772343416194023
4	1.0434285977790578	1.7319870790288416
5	0.4956401158769121	1.0283912554897954
6	0.2718572063437977	0.676883645508492
7	0.1881176787639688	0.5464489203063753
8	0.13403565308162654	0.44497154814559897
9	0.1037472547917067	0.38747264223391964
>10	0.5997106044139636	4.68439823738577
>50	0.06693639144726578	1.9332163238650932
>100	0.1385355057323423	14.724139225351527
>500	0.06256177771975227	18.16949156200671
>1k	0.013868384205041027	7.168443571205387
>5k	4.880799860222364E-5	0.1569017507224953
>10k+	1.3015466293895216E-4	1.17872440230079
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	53258	0.18092920931509915	No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT	47512	0.1614087760144765	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32352	0.1099069018694297	No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31990	0.10867710777704798	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	6.794442499346545E-6	0.0
4	0.0	3.3972212496732724E-6	0.0	1.0191663749019817E-5	0.0
5	0.0	6.794442499346545E-6	0.0	1.358888499869309E-5	0.0
6	0.0	1.0191663749019817E-5	0.0	1.698610624836636E-5	0.0
7	0.0	1.358888499869309E-5	0.0	1.698610624836636E-5	0.0
8	3.3972212496732724E-6	1.698610624836636E-5	0.0	1.698610624836636E-5	0.0
9	3.3972212496732724E-6	1.698610624836636E-5	0.0	2.3780548747712905E-5	0.0
10-11	5.435553999477236E-5	1.698610624836636E-5	0.0	2.3780548747712905E-5	0.0
12-13	2.072304962300696E-4	2.0383327498039634E-5	0.0	2.3780548747712905E-5	0.0
14-15	6.233900993150455E-4	2.0383327498039634E-5	0.0	2.8876380622222816E-5	0.0
16-17	0.00148968151798173	2.5479159372549545E-5	0.0	3.7369433746405994E-5	0.0
18-19	0.0028638575134745685	3.397221249673272E-5	0.0	4.416387624575254E-5	0.0
20-21	0.004728931979545195	3.397221249673272E-5	0.0	4.5862486870589176E-5	0.0
22-23	0.0070645215886955695	3.397221249673272E-5	0.0	4.756109749542581E-5	0.0
24-25	0.009964049925291707	3.397221249673272E-5	0.0	5.435553999477236E-5	0.0
26-27	0.013315408688094391	3.567082312156936E-5	0.0	5.7752761244445625E-5	0.0
28-29	0.016761889645887925	3.7369433746405994E-5	0.0	5.9451371869282265E-5	0.0
30-31	0.019894127638086685	4.076665499607927E-5	0.0	6.11499824941189E-5	0.0
32-33	0.02259152131032726	4.246526562091591E-5	0.0	6.284859311895554E-5	0.0
34-35	0.025127546973208358	4.416387624575254E-5	0.0	6.62458143686288E-5	0.0
36-37	0.027870803132319523	4.756109749542581E-5	0.0	7.134164624313872E-5	0.0
38-39	0.030281131608962713	5.0958318745099084E-5	0.0	7.473886749281199E-5	0.0
40-41	0.032793376723096096	5.0958318745099084E-5	0.0	7.473886749281199E-5	0.0
42-43	0.03576424670593537	5.2656929369935724E-5	3.3972212496732724E-6	7.643747811764862E-5	0.0
44-45	0.03886760831751191	5.7752761244445625E-5	3.3972212496732724E-6	7.813608874248526E-5	0.0
46-47	0.0426504141790231	6.11499824941189E-5	3.3972212496732724E-6	8.323192061699517E-5	0.0
48-49	0.04661836859864148	6.454720374379217E-5	5.0958318745099085E-6	9.172497374117835E-5	0.0
50-51	0.051117988143833726	6.794442499346544E-5	8.493053124183182E-6	9.172497374117835E-5	0.0
52-53	0.0567454851439175	6.794442499346544E-5	1.0191663749019817E-5	9.342358436601499E-5	0.0
54-55	0.06340234018265228	6.794442499346544E-5	1.358888499869309E-5	9.512219499085162E-5	0.0
56-57	0.07177309334184723	6.794442499346544E-5	1.358888499869309E-5	9.512219499085162E-5	0.0
58-59	0.08180508769213239	6.794442499346544E-5	1.358888499869309E-5	9.85194162405249E-5	0.0
60-61	0.09388220923472088	6.794442499346544E-5	1.358888499869309E-5	1.0871107998954472E-4	0.0
62-63	0.10829661899708458	6.794442499346544E-5	1.358888499869309E-5	1.1890274373856453E-4	0.0
64-65	0.12589082784914246	7.473886749281199E-5	1.358888499869309E-5	1.273957968627477E-4	1.6986106248366362E-6
66-67	0.14746318278456771	7.643747811764862E-5	1.698610624836636E-5	1.324916287372576E-4	3.3972212496732724E-6
68-69	0.17315127126397217	7.813608874248526E-5	1.698610624836636E-5	1.3588884998693088E-4	3.3972212496732724E-6
70-71	0.20409486101662117	7.98346993673219E-5	1.698610624836636E-5	1.3588884998693088E-4	3.3972212496732724E-6
72-73	0.24079504217684153	8.832775249150508E-5	1.698610624836636E-5	1.3758746061176753E-4	5.0958318745099085E-6
74-75	0.28373591877271165	9.172497374117835E-5	1.8684716873202997E-5	1.4268329248627744E-4	8.493053124183182E-6
76-77	0.334463226472833	9.172497374117835E-5	2.3780548747712905E-5	1.4777912436078736E-4	1.0191663749019817E-5
78-79	0.39266781814348517	9.172497374117835E-5	2.3780548747712905E-5	1.4947773498562398E-4	1.1890274373856452E-5
80-81	0.4588643728039937	9.512219499085162E-5	2.717776999738618E-5	1.511763456104606E-4	1.358888499869309E-5
82-83	0.5344168747861024	9.512219499085162E-5	2.717776999738618E-5	1.545735668601339E-4	1.358888499869309E-5
84-85	0.6192420921691943	9.512219499085162E-5	2.717776999738618E-5	1.6306661998431707E-4	1.358888499869309E-5
86-87	0.7130937264126682	9.512219499085162E-5	2.717776999738618E-5	1.698610624836636E-4	1.358888499869309E-5
88	0.7890352102278645	9.512219499085162E-5	2.717776999738618E-5	1.7325828373333687E-4	1.358888499869309E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	20560	0.0	40.921814	2
ATCTCGA	24450	0.0	37.140926	1
GGATCCG	25750	0.0	35.223278	5
ATGGATC	26370	0.0	35.181957	3
ATATCTC	26550	0.0	32.767757	1
GATCCGG	40350	0.0	32.125683	1
TCTCGAG	29075	0.0	31.056263	2
TGGATCC	29730	0.0	30.99986	4
TATGGAT	33170	0.0	28.328114	2
CTCGAGG	33815	0.0	26.490406	3
ATATGGA	35980	0.0	26.324306	1
ATCCGGC	50225	0.0	25.759554	2
TCGAGGG	40510	0.0	21.996092	4
TCCGGCG	70965	0.0	18.413967	3
GCGCCGT	80605	0.0	16.020239	9
CCGGCGC	81660	0.0	15.990612	4
CGGCGCG	81610	0.0	15.797882	5
CGTCGAC	79075	0.0	14.627114	12-13
TCGACTT	80955	0.0	14.319711	14-15
GCCGTCG	82470	0.0	14.200431	10-11
>>END_MODULE