FastQCFastQC Report
Wed 25 May 2016
SRR490999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR490999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24327382
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT1142070.4694586536274228No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT957330.39351953284574553No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT483340.19868146930072458No Hit
GATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362080.14883640171392054No Hit
CGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306360.1259321697665618No Hit
TCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289420.11896882286799296No Hit
ATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274380.11278648890373819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC262850.057.2090871
ATGGATC350550.052.4491583
TATCTCG298150.049.5139542
GGATCCG374500.047.898825
TATGGAT395150.046.80942
ATATGGA400050.046.668481
TGGATCC396250.046.2099724
ATCTCGA343550.042.321523
TCTCGAG390350.036.620854
GATCCGG497850.035.9272586
CTCGAGG449550.031.7336245
ATCCGGC586150.030.0497677
TCGAGGG509000.027.8978826
TCCGGCG774100.022.7291138
CGAGGGC659900.021.3474377
CCGGCGC873700.019.9287959
AGGGCGC738450.018.868169
GAGGGCG788800.017.7275968
GCCGGAT1189000.013.7271964
CGGATCC1191550.013.704866
Produced by FastQC (version 0.11.5)